Download or read book Cardiac Cell Culture Technologies written by Zbigniew Brzozka and published by Springer. This book was released on 2017-11-21 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.
Download or read book Cell Culture Techniques in Heart and Vessel Research written by H.M. Piper and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.
Download or read book Advanced Cell Culture Technologies to Boost Cell Based Therapies written by Dominik Egger and published by Frontiers Media SA. This book was released on 2021-09-15 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Correa is the founder of Lumos Biomed Consulting and holds shares in Cryovida Stem Cell Bank (Mexico). Dr O’Brien holds patents related to regeneration technology and was a co-founder of SurgaColl Technologies. All other Topic Editors declare no competing interests with regard to the Research Topic subject.
Download or read book Technologies in Cell Culture written by and published by BoD – Books on Demand. This book was released on 2024-06-26 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technologies in Cell Culture - A Journey From Basics to Advanced Applications is a comprehensive book that offers a broad overview of the subject, encompassing fundamental concepts, modern techniques, and their diverse applications across various fields. Comprising eleven chapters authored by leading international experts in their respective fields, this book adeptly navigates the complexities of cell culture. It provides valuable insights into bioprocessing, cancer biology, regenerative medicine, and more. The book explores innovative strategies for restoring eyesight in individuals with age-related macular degeneration through retinal pigment epithelium monolayers derived from autologous adipose tissue stem cells. We discover novel approaches for utilizing in vitro techniques to evaluate new medications targeting crucial molecular pathways. A deeper comprehension of the tumor microenvironment can be achieved by conducting coculture studies on cancer cells and adipocytes. The readers will gain insight into organoid intelligence, a culmination of advancements in stem cell technologies, bioengineering, and artificial intelligence. For anyone intrigued by unraveling the mysteries of cellular life, Technologies in Cell Culture - A Journey From Basics to Advanced Applications is an indispensable resource, catering to both novice scientists and seasoned researchers seeking to expand their knowledge. Join us on this journey as we explore the myriad ways in which cell culture technology can impact biomedicine and beyond.
Download or read book Design Fabrication and Testing of a Microfluidic System for Cardiac Cell Culture written by Mai-Dung Thi Nguyen and published by . This book was released on 2010 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiovascular disease (CVD) is the leading cause of death in the United States and accounts for nearly 1,372,000 deaths each year. In addition, ~81 million Americans suffer from some form of CVD. Understanding the molecular basis of various manifestations of CVD requires cellular-level studies. However, current technologies for cell culture, fail to recreate the in-vivo environment where cells are subject to pressure and stretch as a consequence of normal hemodynamic loading and unloading. Therefore, to study cardiac cells with physiological relevance, the mechanical loading environment needs to be accurately replicated in-vitro. In order to create an appropriate platform for cardiac cell culture, a micro fluidic cardiac cell culture model ( CCCM) was designed and fabricated. This system consists of a pump, cell culture chamber, pneumatically actuated collapsible valve and a tunable resistance element in series. By varying the pump flow rate, valve closure frequency and the outflow resistance, various conditions associated with normal and dysfunctional heart function were recreated. A rat left ventricle heart muscle cell line (H9c2) was used to establish proof-of-concept and demonstrate the ability of the CCCM to sustain cell culture under normal physiological conditions. Microscopic evaluation of these cells using phase contrast and immunofluoresence demonstrated that cells cultured within the CCCM achieved an in-vivo like phenotype in comparison to static unloaded controls.
Download or read book Advanced Technologies in Cardiovascular Bioengineering written by Jianyi Zhang and published by Springer Nature. This book was released on 2022-02-05 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a systematic overview of the technologies currently being explored and utilized in the fields of cardiovascular tissue engineering and regenerative medicine. Considering the unprecedented rapid progress occurring on multiple technological fronts in cardiac tissue engineering, this important new volume fills a need for an up-to-date, comprehensive text on emerging advanced biological and engineering tools. The book is an important resource for anyone looking to understand the emerging topics that have the potential to substantially influence the future of the field. Coverage includes iPS stem cell technologies, nanotechnologies and nanomedicine, advanced biomanufacturing, 3D culture systems, 3D organoid systems, genetic approaches to cardiovascular tissue engineering, and organ on a chip. This book will be a valuable guide for research scientists, students, and clinical researchers in the fields of cardiovascular biology, medicine, and bioengineering, as well as industry-based practitioners working in biomaterial science, nanomaterials and technology, and rapid prototyping and biomanufacturing (3D bioprinting).
Download or read book Multiscale Technologies for Human Stem Cell Culture in Suspension written by Monica Zagallo and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Emerging Technology Platforms for Stem Cells written by Uma Lakshmipathy and published by John Wiley & Sons. This book was released on 2009-04-06 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with their use for drug delivery.
Download or read book Culturing Life written by Hannah Landecker and published by Harvard University Press. This book was released on 2010-03-30 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: How did cells make the journey, one we take so much for granted, from their origin in living bodies to something that can be grown and manipulated on artificial media in the laboratory, a substantial biomass living outside a human body, plant, or animal? This is the question at the heart of Hannah Landecker's book. She shows how cell culture changed the way we think about such central questions of the human condition as individuality, hybridity, and even immortality and asks what it means that we can remove cells from the spatial and temporal constraints of the body and "harness them to human intention." Rather than focus on single discrete biotechnologies and their stories--embryonic stem cells, transgenic animals--Landecker documents and explores the wider genre of technique behind artificial forms of cellular life. She traces the lab culture common to all those stories, asking where it came from and what it means to our understanding of life, technology, and the increasingly blurry boundary between them. The technical culture of cells has transformed the meaning of the term "biological," as life becomes disembodied, distributed widely in space and time. Once we have a more specific grasp on how altering biology changes what it is to be biological, Landecker argues, we may be more prepared to answer the social questions that biotechnology is raising.
Download or read book Fabrication of a MOSFET Cardiac Cell Culture Array written by Vincent Maurice McNeil and published by . This book was released on 1985 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stem Cell Systems Bioengineering written by Tiago G. Fernandes and published by Frontiers Media SA. This book was released on 2021-07-02 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Stephanie Willerth has a commercialization agreement with Aspect Biosystems with regards to bioprinting stem cell derived tissues. Dr. Yuguo Lei is a co-founder of CellGro Technologies, LLC, a company focusing on cell expansion technologies. Dr. Tiago Fernandes has no competing interests with regards to this Research Topic.
Download or read book Microfluidic Cell Culture Systems written by Jeffrey T Borenstein and published by Elsevier. This book was released on 2018-09-12 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. - Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. - Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. - Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.
Download or read book Cell Culture Technology for Pharmaceutical and Cell Based Therapies written by Sadettin Ozturk and published by CRC Press. This book was released on 2005-08-30 with total page 788 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel
Download or read book The Second Generation of the CCCM System for In vitro Cardiac Tissue Engineering written by Mai-Dung Thi Nguyen and published by . This book was released on 2014 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiovascular disease is the leading cause of death worldwide. When a myocardial infarction occurs, scar tissue compensates the damaged myocardial tissue. This scar tissue increases the stiffness of the heart tissue, reduces the heart's function, and finally leads to the heart failure (HF) disease. To have the tissue engraftment, in-vitro cardiac tissue should have the same properties as the native mature cardiac tissue. However, current in-vitro cell culture technologies fail to accurately recreate the in-vivo like mechanically physiological environment for in-vitro cardiac tissue culture, and therefore, fail to regenerate the in-vivo like mature cardiac tissue. Hence, a microfluidic cardiac cell culture model (CCCM) system was developed to better recreate the cellular environment and advance cardiac regeneration. CCCM system replicates the hemodynamic loading and unloading conditions occurring inside the left ventricle of a heart. With this system, different pressures of human heart conditions may be replicated for a variety of clinical and physiologic conditions. For proof-of-concept, embryonic chick cardiac cells with normal heart condition were applied. Compared to the tissue cultured in a static condition, tissues stimulated in the CCCM system achieved an in-vivo like cardiac matured phenotype, had higher proliferating rate, showed more maturity, and expressed more contractile proteins. These results demonstrated that the CCCM system can be used to study the behavior of cardiomyocytes in different mechanical heart conditions and to create mature cardiac tissue which will benefit cardiac tissue transplant for HF.
Download or read book Microelectronic Fabrication Process for MOSFET Cardiac Cell Culture Array written by Michael Scott Cafferty and published by . This book was released on 1983 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cardiac Tissue Engineering written by Smadar Cohen and published by Springer Nature. This book was released on 2022-06-01 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac tissue engineering aims at repairing damaged heart muscle and producing human cardiac tissues for application in drug toxicity studies. This book offers a comprehensive overview of the cardiac tissue engineering strategies, including presenting and discussing the various concepts in use, research directions and applications. Essential basic information on the major components in cardiac tissue engineering, namely cell sources and biomaterials, is firstly presented to the readers, followed by a detailed description of their implementation in different strategies, broadly divided to cellular and acellular ones. In cellular approaches, the biomaterials are used to increase cell retention after implantation or as scaffolds when bioengineering the cardiac patch, in vitro. In acellular approaches, the biomaterials are used as ECM replacement for damaged cardiac ECM after MI, or, in combination with growth factors, the biomaterials assume an additional function as a depot for prolonged factor activity for the effective recruitment of repairing cells. The book also presents technological innovations aimed to improve the quality of the cardiac patches, such as bioreactor applications, stimulation patterns and prevascularization. This book could be of interest not only from an educational perspective (i.e. for graduate students), but also for researchers and medical professionals, to offer them fresh views on novel and powerful treatment strategies. We hope that the reader will find a broad spectrum of ideas and possibilities described in this book both interesting and convincing. Table of Contents: Introduction / The Heart: Structure, Cardiovascular Diseases, and Regeneration / Cell Sources for Cardiac Tissue Engineering / Biomaterials: Polymers, Scaffolds, and Basic Design Criteria / Biomaterials as Vehicles for Stem Cell Delivery and Retention in the Infarct / Bioengineering of Cardiac Patches, In Vitro / Perfusion Bioreactors and Stimulation Patterns in Cardiac Tissue Engineering / Vascularization of Cardiac Patches / Acellular Biomaterials for Cardiac Repair / Biomaterial-based Controlled Delivery of Bioactive Molecules for Myocardial Regeneration
Download or read book Drug Testing In Vitro written by Uwe Marx and published by Wiley-Blackwell. This book was released on 2007-02-27 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, expert scientists from industry and academia share their knowledge on the assembly of functional human tissues in vitro and how to design drug screenings predictive of human exposure. In so doing, they combine the latest technological developments with strategic outlooks, such as novel cell and tissue systems for drug screening and testing, as well as emerging in vitro culture technologies. Equally importantly, the book does not shy away from regulatory acceptance and ethical issues.
