Download or read book Caged Compounds written by Gerard Marriott and published by Academic Press. This book was released on 1998-06-22 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Download or read book High Content Screening written by D. Lansing Taylor and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: There has always been some tension between proponents of hypothesis-driven and discovery-driven research in the broad field of life sciences. Academic research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery research in the pharmaceutical industry has embraced discovery-driven research for many years. A good example has been the discovery of active compounds from large chemical libraries, through screening campaigns. The success of the human genome project has also demonstrated the need for both academic researchers and industrial researchers to now understand the functions of genes and gene products. The cell is the basic unit of life and it has been at the cellular level where function can be demonstrated most cost-effectively and rapidly. High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell.
Download or read book Development of photoactivatable caged compounds for biological applications written by Dominga Rogolino and published by . This book was released on 2002 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Synthesis of Caged NADP Compounds for Use in the Time resolved X ray Crystallographic Study of Isocitrate Dehydrogenase written by Bruce Elliot Cohen and published by . This book was released on 1996 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Carbocyclic Cage Compounds written by Eiji Ōsawa and published by VCH Publishers. This book was released on 1992 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nitric Oxide written by Lester Packer and published by Gulf Professional Publishing. This book was released on 1996 with total page 598 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Encapsulation Phenomenon written by Yan Voloshin and published by Springer. This book was released on 2016-04-27 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fundamental book presents the most comprehensive summary of the current state of the art in the chemistry of cage compounds. It introduces different ways of how ions and molecules can be encapsulated by three-dimensional caging ligands to form molecular and polymeric species: covalent, supramolecular, and coordination capsules. The authors introduce their classification, reactivity, and selected practical applications. Because encapsulation can isolate caged ions and molecules from external factors, the encapsulated species can exhibit unique physical and chemical properties. The resulting specific reactivity and selectivity can open up a range of applications, including chemical separation, recognition, chiral separation, catalysis, applications as sensors or probes, as molecular or supramolecular devices, or molecular carriers (cargo).A particularly strong emphasis in this book is on the summary and review of the synthesis of various types of cage compounds. Readers will find over 850 literature references summarized and clearly represented in over 600 schemes and illustrations. The book is structured by the types of caging ligands (covalent, supramolecular, or coordination capsules). The authors further arranged the chapters by ligand classes and types of encapsulated species (neutral molecules, anions, or cations). Readers will hence find an exhaustive reference resource and summary of the current state of research into encapsulated species, nowadays almost a separated realm of modern chemistry.
Download or read book Photochemistry of Organic Compounds written by Petr Klán and published by John Wiley & Sons. This book was released on 2009-03-23 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: Photochemistry of Organic Compounds: From Concepts to Practice provides a hands-on guide demonstrating the underlying principles of photochemistry and, by reference to a range of organic reaction types, its effective use in the synthesis of new organic compounds and in various applications. The book presents a complete and methodical approach to the topic, Working from basic principles, discussing key techniques and studies of reactive intermediates, and illustrating synthetic photochemical procedures. Incorporating special topics and case studies covering various applications of photochemistry in chemistry, environmental sciences, biochemistry, physics, medicine, and industry. Providing extensive references to the original literature and to review articles. Concluding with a chapter on retrosynthetic photochemistry, listing key reactions to aid the reader in designing their own synthetic pathways. This book will be a valuable source of information and inspiration for postgraduates as well as professionals from a wide range of chemical and natural sciences.
Download or read book Chemical Studies of Caged Compounds written by Belagaje Ramamoorthy and published by . This book was released on 1971 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optochemical Biology written by and published by Academic Press. This book was released on 2019-07-30 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on optochemical biology
Download or read book Bioanalytics written by Friedrich Lottspeich and published by John Wiley & Sons. This book was released on 2018-03-08 with total page 1810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
Download or read book Dynamic Studies in Biology written by Maurice Goeldner and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers. A must-have for every biochemist, biophysicist and molecular biologist developing and working with these novel methods.
Download or read book Green Science and Technology written by Enoch Y. Park and published by CRC Press. This book was released on 2019-10-16 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive and up-to-date review of recent trends of green science and technology. Worldwide deterioration of environment and global warming threaten our lifestyle and the survival of all creatures. In order to weather these problems, we need to construct a multidisciplinary approach involving the fusion of various advanced researches. The book begins with an overview on fundamental research about generation and utilization of renewable energy, protection of the earth's ecosystem for better coexistence with nature, development of artificial intelligence-based agriculture and molecular recognitionbased welfare and covers a wide range of innovative research on green science and technology.
Download or read book Time resolved Macromolecular Crystallography written by Royal Society (Great Britain) and published by Oxford [England] ; New York : Oxford University Press. This book was released on 1992 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: The papers presented in this volume report the striking progress X-ray diffraction has facilitated in the study of structural molecular biology. Coupled with the revival of the Laue method, the advent of high-intensity synchrotron radiation sources has made possible the rapid collection of X-ray crystallography data, thereby allowing protein and virus crystallography to progress from studies of equilibrium structures to time-resolved studies of structures at reaction stages. The book also details the many recent technological developments in physics, chemistry and biochemistry that have been critical for the full exploitation of the synchrotron Laue method in the study of dynamic events in crystals. Necessary future developments are discussed.
Download or read book Synthesis and Computational Studies of Photoreactive Caged Molecules written by Yannick Patekede Ouedraogo and published by . This book was released on 2012 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: In photoreactive caged compounds, a molecule of interest is covalently coupled to a protection group, making it functionally inactive. Upon irradiation, the molecule of interest is released and becomes active to perform biological functions. Caged neurotransmitters are of increasing interest in the neuroscience community. Despite the significant progress in biological optical and irradiation (lasers) techniques and equipment, caged neurotransmitters remain challenging to synthesize, and their mechanism of photolysis stays ambiguous. Our initial approach was to improve the synthesis of 4-carboxymethoxy-5,7-dinitroindolinyl (CDNI) Glu, as previously established by the Ellis-Davies and Corrie groups. By combining several deprotection steps and tweaking the procedure, we shortened the synthesis from 150 hours to just 24 hours, while improving the overall yields. The improved synthetic method for nitrated caged compounds enabled us to make and characterize caged GABA and acyl analogues. Subsequently, we designed and synthesized other novel cages such as di-caged-Glu, di-Glu and di-GABA cages to address phototoxicity and solubility issues commonly reported in nitrated cages. We also compared the kinetics of several caged neurotransmitters by conventional UV-Vis studies, and developed a quantitative 1H NMR method for relative kinetics. While allowing for a small sample size for analysis, the UV-Vis technique was ambiguous because of overlapping absorbance spectra in certain caged molecules and remnant cages upon photolysis. The 1H NMR method yielded more detailed kinetic data, and was correlated to the UV-Vis method. The ratio of the rate constants for the photolysis of CDNI-Glu vs. MNI-Glu as 1.3 for both UV-Vis and NMR methods; the ratio of reaction rates was 7.0 for the NMR method. HPLC purified samples were successfully tested on brain slices using two-photon microscopy. To further investigate the mechanism of photolysis, theoretical studies were conducted on CDNI-Glu. Most previous mechanistic reports made assumptions that neglect the effect of the CDNI side chains and nature of the neurotransmitter. We successfully studied the mechanism of photolysis of CDNI-Glu in aqueous and organic media at the B3LYP 6-31G* level of density functional theory (DFT). Key controversial pathways (organic vs. aqueous, long vs. short range rearrangements) were compared and found to be thermodynamically plausible; these were also correlated to our experimental UV-Vis and NMR kinetics results.
Download or read book Pumps Channels and Transporters written by Ronald J. Clarke and published by John Wiley & Sons. This book was released on 2015-09-16 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes experimental methods for investigating the function of pumps, channels and transporters Covers new emerging analytical methods used to study ion transport membrane proteins such as single-molecule spectroscopy Details a wide range of electrophysiological techniques and spectroscopic methods used to analyze the function of ion channels, ion pumps and transporters Covers state-of-the art analytical methods to study ion pumps, channels, and transporters, and where analytical chemistry can make further contributions
Download or read book Stomata written by M. Fricker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this popular work provides a comprehensive account of all aspects of stomatal biology. The substantially revised text is thoroughly up to date and well illustrated with numerous line illustrations, photographs and comprehensive tables. The theory of gaseous diffusion through stomata is reviewed in a new chapter and sections on signal perception and transduction, guard cell ionic relations and guard cell metabolism have been added. A concluding chapter reviews the genetics and molecular biology of stomata. This work provides a comprehensive reference text which will appeal to advanced students, post-graduates and lecturers in plant physiology.
