Download or read book Biomedical Light Microscopy written by J. James and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: New interest in light microscopy of the last few years has not been backed up by adequate general literature. This book intends to fill the gap between specialized texts on detailed topics and general introductory booklets, mostly dealing with the use of the conventional light microscope only. In this short textbook both new developments in microscopy and basic facts of image formation will be treated, including often neglected topics such as axial resolving power, lens construction, photomicrography and correct use of phase-en interference contrast systems. Theoretical background will be dealt with as far as necessary for a well-considered application of these techniques enabling a deliberate choice for the approach of a certain problem. Over 150 illustrations (photomicrographs and diagrams) complete the information on microscopy of the nineties in the biomedical field, intended for scientists, doctors, technicians and research students. Many drawings have been contributed by the illustrator R. Kreuger; the photographic work has been executed by J. Peeterse. Secretarial assistance in preparing the manuscript was given by Ms T. M. S. Pierik. Dr M. J. Pearson has corrected the English of the final text.
Download or read book Techniques In Microscopy For Biomedical Applications written by Terje Dokland and published by World Scientific Publishing Company. This book was released on 2006-09-28 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.
Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ideal for cell biologists, life scientists, biomedical engineers, and clinicians, this handbook provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy.
Download or read book Handbook of Biomedical Optics written by David A. Boas and published by CRC Press. This book was released on 2016-04-19 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamental
Download or read book Biomedical Optical Phase Microscopy and Nanoscopy written by Natan T. Shaked and published by Academic Press. This book was released on 2012-11-05 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.
Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.
Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
Download or read book Wavefront Shaping for Biomedical Imaging written by Joel Kubby and published by Cambridge University Press. This book was released on 2019-06-20 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn about the theory, techniques and applications of wavefront shaping in biomedical imaging using this unique text. With authoritative contributions from researchers who are defining the field, cutting-edge theory is combined with real-world practical examples, experimental data and the latest research trends to provide the first book-level treatment of the subject. It is suitable for both background reading and use in a course, with coverage of essential topics such as adaptive optical microscopy, deep tissue microscopy, time reversal and optical phase conjugation, and tomography. The latest images from the forefront of biomedical imaging are included, and full-colour versions are available in the eBook version. Researchers, practitioners and graduate students in optics, biophotonics, biomedical engineering, and biology who use biomedical imaging tools and are looking to advance their knowledge of the subject will find this an indispensable resource.
Download or read book Technical developments and biomedical applications of an instrument combining light microscopy and scanning electron microscopy written by Cornelia H. Wouters and published by . This book was released on 1986 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microscopy Techniques for Biomedical Education and Healthcare Practice written by Leonard Shapiro and published by Springer Nature. This book was released on 2023-11-02 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book has a strong focus on advances in microscopy that straddles research, medical education and clinical practice. These advances include the shift in power from conventional to digital microscopy. The first section of this book covers imaging techniques and morphometric image analysis with its applications in biomedicine using different microscopy modes. Chapters highlight the rich development of fluorescence methods and technologies; particle tracking techniques with applications in biomedical research and nanomedicine; the way in which visualizations have revolutionized taxonomy from gross anatomy to genetics; and the psychology of perception and how it affects our understanding of cells and tissues. The book’s first section concludes by exploring the use of CT modalities to evaluate anterior deformities in craniosynostosis. In the second section of the book, chapters on anatomical and cell biology education explore the history of anatomical models and their use in educational settings. This includes examples in 3D printing and functional human anatomical models that can be created using easily available resources and the use of biomedical imaging in visuospatial teaching of anatomy; the novel use of ultrasound in medical education and practice; and skill acquisition in histology education using a flowchart called a ‘decision tree’. This book will appeal to histologists, microscopists, cell biologists, clinicians and those involved in anatomical education and biomedical visualization, as well as students in those respective fields.
Download or read book Atomic Force Microscopy written by Pier Carlo Braga and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).
Download or read book Quantitative Biomedical Optics written by Irving J. Bigio and published by Cambridge University Press. This book was released on 2016-01-07 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: Based on physical science principles, Quantitative Biomedical Optics covers theory, instrumentation, methods and applications, with practical exercises and problem sets.
Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.
Download or read book Introductory Biomedical Imaging written by Bethe A. Scalettar and published by CRC Press. This book was released on 2022-09-08 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imaging is everywhere. We use our eyes to see and cameras to take pictures. Scientists use microscopes and telescopes to peer into cells and out to space. Doctors use ultrasound, X-rays, radioisotopes, and MRI to look inside our bodies. If you are curious about imaging, open this textbook to learn the fundamentals. Imaging is a powerful tool in fundamental and applied scientific research and also plays a crucial role in medical diagnostics, treatment, and research. This undergraduate textbook introduces cutting-edge imaging techniques and the physics underlying them. Elementary concepts from electromagnetism, optics, and modern physics are used to explain prominent forms of light microscopy, as well as endoscopy, ultrasound, projection radiography and computed tomography, radionuclide imaging, and magnetic resonance imaging. This textbook also covers digital image processing and analysis. Theoretical principles are reinforced with illustrative homework problems, applications, activities, and experiments, and by emphasizing recurring themes, including the effects of resolution, contrast, and noise on image quality. Readers will learn imaging fundamentals, diagnostic capabilities, and strengths and weaknesses of techniques. This textbook had its genesis, and has been vetted, in a "Biomedical Imaging" course at Lewis & Clark College in Portland, OR, and is designed to facilitate the teaching of similar courses at other institutions. It is unique in its coverage of both optical microscopy and medical imaging at an intermediate level, and exceptional in its coverage of material at several levels of sophistication.
Download or read book Methods of Preparation for Electron Microscopy written by David G. Robinson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by . This book was released on 2019 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology - New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
