Download or read book Biocatalytic Productions of Amino Acids and Derivatives written by David Rozzell and published by . This book was released on 1992-01-01 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biocatalytic Production of Amino Acids and Derivatives written by J. David Rozzell and published by . This book was released on 1992-01-01 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biocatalytic Preparation of Amino Acids written by Vivek Kothari and published by Daya Books. This book was released on 2007 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amino Acids Are The Molecular Units That Make Up Proteins. All Proteins Are Various Compositions Of Twenty Specific Naturally Occurring Amino Acids. Amino Acids Are Found In All Foods Except Oil. There Are About Eighty Amino Acids Found In Nature, But Only Twenty To Twenty-Nine Are Required For Human Growth. Amino Acids Play A Pivotal Role In Living Systems, And Therefore Synthetic Amino Acids Have Been Of Significant Interest To Researchers Working Towards The Understanding And Modification Of Physiological Processes. Currently, The Amino Acids Used In Amino Acid Products Are Mainly Manufactured By Biocatalytic Methods. The Book Presents Detailed Discussion Of The Preparation Of Amino Acids By Biocatalysis. It Provides Examples Of Recently Developed Biocatalytic Methods For The Production Of Amino Acids And Its Derivatives. It Offers Upper Level Students And Professionals An Essential Resource For Pharmaceutical Development, Medicinal Chemistry And Biochemistry. Contents Chapter 1: Basics Of Biocatalysis; Chapter 2: Production Of Amino Acids; Chapter 3: Asymmetric Synthesis Of Amino Acids; Chapter 4: Amino Acids And Derivatives; Chapter 5: Strategies For Efficient Biocatalysis; Chapter 6: Synthesis Of Cage Amino Acids; Chapter 7: Types Of B-Amino Acids; Chapter 8: Extremophiles And Extremozymes; Chapter 9: Immobilised Enzymes; Chapter 10: Enzymatic Peptide Synthesis; Chapter 11: Synthesis Of Enantiomerically Pure (S)-Amino Acids And (R)-Amines; Chapter 12: Cloning And Sequence Analysis; Chapter 13: Bioseparation And Biocatalytic Applications.
Download or read book Catalytic and Biocatalytic Methods for the Efficient Synthesis of Biologically Relevant Non Proteinogenic Amino Acids written by Anke Lemke and published by Cuvillier Verlag. This book was released on 2014-04-29 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-proteinogenic amino acids are often important constituents of biologically potent natural products. The synthesis of these amino acids is often time-consuming, non-trivial and not sustainable. Thus, the central topic of this work was the formation of unusual amino acid structures by biocatalytic and catalytic methods. For the elucidation of the biosynthetic pathway of Streptomyces-produced muraymycins and related natural products, a ‘biosynthetic tool kit’ was compiled and prepared. This does not only comprehend the completed synthesis of deuterium-labeled compounds, such as (3R)- and (3S)-3-hydroxy-[5-2H]-l-arginine and other potential intermediates with a nucleosidic structure, but it also includes the development of fermentation methodology including the possibility to detect the muraymycin derivatives by LC MS. Furthermore, different synthetic routes with catalytic key steps for the preparation of the non-proteinogenic amino acid enduracididine were investigated.
Download or read book Biocatalytic Synthesis of Bioactive Compounds written by Josefina Aleu and published by MDPI. This book was released on 2020-12-02 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalysis, the application of enzymes as catalysts for chemical synthesis, has become an increasingly valuable tool for the synthetic chemist. Enzymatic transformations carried out by enzymes or whole-cell catalysts are used for the production of a wide variety of compounds ranging from bulk to fine chemicals. The primary consideration for the incorporation of biotransformation in a synthetic sequence is regio- and stereocontrol that can be achieved with enzyme-catalyzed reactions. Biotransformations are thus becoming accepted as a method for generating optically pure compounds as well as for developing efficient routes to target compounds. This Special Issue aims to address the main applications of biocatalysts, isolated enzymes, and whole microorganisms in the synthesis of bioactive compounds and their precursors.
Download or read book Novel Enzyme and Whole Cell Biocatalysts written by Anwar Sunna and published by MDPI. This book was released on 2020-11-04 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
Download or read book Biocatalytic Synthesis of gamma hydroxy Amino Acids and Computational Exploration of Sequence activity Landscapes written by Jonathan Michael Ellis and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Through the course of evolution, Nature has developed a swath of useful enzymes for catalyzing chemical reactions crucial to life. These enzymes are used in complex metabolic cascades to produce a wide variety of useful natural products in a chemo-, regio-, and stereoselective fashion. These enzymes can be adopted in synthetic chemistry contexts to afford powerful tools for synthesizing complex molecules in a green way. Through protein engineering, it is possible to further expand the applicability of these enzymes, and by using computational modelling tools, it is possible to accelerate the engineering process. Here, I discuss my efforts in engineering two useful enzymes pyridoxal 5'-phosphate-dependent enzymes, UstD and RgnTDC, to expand their substrate scopes and applicability. UstD is capable of directly installing an amino acid moiety into electrophilic aldehyde substrates through a decarboxylative aldol-like reaction from L-aspartic acid. Through multiple rounds of directed evolution, including linear regression guided evolution, we were able to improve the activity and expressibility of UstD. Through mechanistic studies and crystallography, we have begun probing the complex catalytic cycle that enables UstD to perform its catalyzed reaction, as well as laying the foundation for structure guided evolution. Complementary to UstD, RgnTDC is a l-tryptophan decarboxylase which is able to decarboxylate amino acids and generate precursors to medicinally relevant compounds. Through the use of Substrate Multiplexed Screening (SUMS) in conjunction with logistic regression guided directed evolution and primer-doped mutagenesis, we have created a variety of generalist biocatalysts with diverse active site composition. These variants serve as potential starting points for future engineering campaigns. To help facilitate these future engineering efforts and explore the utility of SUMS, I have also worked to develop computational tools to process and analyze SUMS library screening data. These tools have been integrated into a single web-based program, titled the SUMS Interactive Processing Software (SIPS), and have been developed with broad applicability and community use in mind.
Download or read book Modification of Polymers written by Charles E. Carraher and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: The sheer volume of topics which could have been included under our general title prompted us to make some rather arbitrary decisions about content. Modification by irradiation is not included because the activity in this area is being treated elsewhere. We have chosen to emphasize chemical routes to modification and have striven to pre sent as balanced a representation of current activity as time and page count permit. Industrial applications, both real and potential, are included. Where appropriate, we have encouraged the contributors to include review material to help provide the reader with adequate context. The initial chapter is a review from a historical perspective of polymer modification and contains an extensive bibliography. The remainder of the book is divided into four general areas: Reactions and Preparation of Copolymers Reactions and Preparation of Block and Graft Copolymers Modification Through Condensation Reactions Applications The chemical modification of homopolymers such as polyvinylchlo ride, polyethylene, poly(chloroalkylene sulfides), polysulfones, poly chloromethylstyrene, polyisobutylene, polysodium acrylate, polyvinyl alcohol, polyvinyl chloroformate, sulfonated polystyrene; block and graft copolymers such as poly(styrene-block-ethylene-co-butylene block-styrene), poly(I,4-polybutadiene-block ethylene oxide), star chlorine-telechelic polyisobutylene, poly(isobutylene-co-2,3-dimethyl- 1,3-butadiene), poly(styrene-co-N-butylmethacrylate); cellulose, dex tran and inulin, is described.
Download or read book Biocatalysis written by D.A. Abramowicz and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: The action of enzymes fascinated mankind long before they were rec ognized for the complex chemicals that they are. The first application of these remarkable compounds to produce ethanol by fermentation is lost to antiquity. Payer and Persoz (Ann. Chim. Phys. , 53, 73 (1833ii)) appear to have provided the first step toward understanding this com plex area when they reported the isolation of diastase in 1833. These workers showed that diastase could catalyze the hydrolysis of starches to sugars. Somewhat earlier Kirchhoff (Schwigger's Journal, 4, 108 (1812)) had shown that a small amount of dilute acid could hydrolyze a seemingly endless amount of starch to sugars. The genius of Berzelius recognized the commonality of these two observations in connection with a few other isolated observations and in 1834 coined the term catalysis to describe such actions. Professor Leibig was one of the giants of the chemical world in 1840. In addition to his own work, Liebig was training the world's next generation of chemists in his laboratory in Giessen. This cadre of chemists were very impressed by the master teacher so that is it only natural that Liebig's views should dominate with this next generation of chemists. Leibig was, in the 1830s and 1840s, developing his mastery of agricultural chemistry. The mechanism of putrefication was of great concern to Leibig, and he turned to the newly defined area of catalysis for an explanation.
Download or read book Development of Bioprocesses for the Production of Biocatalysts Used in the Synthesis of Amino Acids and Amines written by Brian Hugh Wilson Griffin and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research in to enzyme catalysis has enormously enriched the success of biological organic synthesis; particularly in the last decade, remarkable progress in biological catalysis has taken place leading to the application of enzymes to a wider extent in industrial processes. The benefits of establishing enzymes as industrial catalysts are founded on the need to develop a 'clean' and 'pure' technology which carry out isomer and region selective reactions; generate or transform pure isomers compared with racemic mixtures and produce pure compounds in comparison with mixtures of by-products often arising from conventional chemical synthesis. With the onset of molecular biology and genetics, the manufacturing of high value proteins in recombinant host cells has become commonplace; nevertheless, there needs to be a better understanding of the optimum conditions for the production of every recombinant protein. In this study, the over expression of an industrially important amine oxidase - D-Amino Acid Oxidase (DAAO) - was attempted using two different expression systems: Escherichia coli and Pichia pastoris in order to establish the optimum conditions for industrial production. Amine oxidases exhibit stereoselectivity; ergo they can theoretically be used for the deracemisation of nonopticallypure mixtures of amines leading to optically pure amines of high value.Furthermore, DAAO is involved in the production of 7-aminocephalosporanic acid (7-ACA) from cephalosporin c (CPC), which can be used in the synthesis of novel cephalosporin antibiotic derivatives. In order to accelerate development of these new antibiotics DAAO must be readily available. Overexpression of DAAO in E. coli was studied under varying experimental conditions in order to ascertain the best conditions for enzymatic activity whilst simultaneously investigating the variations between both defined and complex media to understand suitability for scale-up.Although E. coli is still regarded as the 'go to' organisms for initial laboratory scale up the methylotrophic yeast P. pastoris is considered an industrial workhorse for the production of recombinant proteins and thus the expression of DAAO was also investigated to ascertain how enzyme activities are affected by this expression system. Furthermore, the supplementation of media with different nutrient sources of varying complexity was investigated, as was the implementation of co-feeding inorder to improve carbon flux during recombinant protein production. Finally, the oxygen availability was investigated in order to gain important understandings on its effects on microbial physiology and DAAO activity.
Download or read book Biocatalytic Synthesis of Pharmaceutically Relevant Amides and Amino Acids written by and published by . This book was released on 2021 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Multi Step Enzyme Catalysis written by Eduardo Garcia-Junceda and published by John Wiley & Sons. This book was released on 2008-11-21 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first comprehensive coverage of this unique and interdisciplinary field provides a complete overview, covering such topics as chemoenzymatic synthesis, microbial production of DNA building blocks, asymmetric transformations by coupled enzymes and much more. By combining enzymatic and synthetic organic steps, the use of multi-enzyme complexes and other techniques opens the door to reactions hitherto unknown, making this monograph of great interest to biochemists, organic chemists, and chemists working with/on organometallics, as well as catalytic chemists, biotechnologists, and those working in the pharmaceutical and fine chemical industries.
Download or read book Towards Industrial Production of a Useful Biocatalyst for Amino Acid Synthesis written by Oluwasola Okor and published by . This book was released on 2005 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Non a Amino Acid Synthesis written by Michael Smith and published by CRC Press. This book was released on 1995-05-17 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work provides a comprehensive overview of, and the most common and useful methods for, the synthesis of non-alpha-amino acids, particularly amino acids that are key synthetic intermediates or important compounds in their own right. Attention is paid to acyclic amino acids C3-C10 including amino alkanoic carboxylic acids, aminoalkenoic acids and aminoalkynoic acids.
Download or read book Novel Biocatalytic Strategies for the Synthesis of N functionalised Amino Acids written by Julia Hyslop and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Discovery and Properties of Enzymes for Biocatalytic Production of beta amino Acids written by Rijksuniversiteit Groningen and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biocatalytic Asymmetric Synthesis of Unnatural Amino Acids Using C N Lyases written by and published by . This book was released on 2019 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt:
