Download or read book Avidin biotin Technology written by Meir Wilchek and published by . This book was released on 1990 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.
Download or read book Protein Patterning Strategies Exploiting Avidin biotin Technology written by and published by . This book was released on 1997 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Enzymology written by and published by . This book was released on 1990 with total page 746 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Avidin Biotin Interactions written by Robert J. McMahon and published by Springer Science & Business Media. This book was released on 2008-02-26 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.
Download or read book Immobilised Macromolecules Application Potentials written by U.B. Sleytr and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized functional biomolecules, particularly enzymes, are important tools in biotechnology, biochemistry, biochemical engineering, biomedicine and biosensor research. This book provides an introduction and overview of selected major areas of the science and technology of immobilized systems. The chapters are intended as an introduction and overview to these interdisciplinary areas, as well as a source of practical details and of new research trends. This book will be useful for scientists, technologists, academics and students in direct and related fields.
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Download or read book Immunoenzymatic Techniques written by S. Avrameas and published by Elsevier Science & Technology. This book was released on 1983 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aandacht voor de enzymatische-immunocytochemie met de nadruk op intracellulaire penetratie van gelabelde antilichamen en de toepassing van "double staining"-methodes of monoclonale antilichamen in normale en in diverse experimentele of pathologische omstandigheden; kwantitatieve aspecten van immuno-enzymatische technieken, zoals nieuwe technische vindingen en toepassingen op verschillende gebieden, bijvoorbeeld biologie, humane en veterinaire geneeskunde, fytopathologie en experimentele ziekten
Download or read book Graphene Field Effect Transistor Biosensors written by Shiyu Wang and published by Springer Nature. This book was released on 2021-03-31 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this monograph, the graphene-based field-effect transistor (FET) biosensors are shown to be an emerging sensing platform. Divided into two parts the first set of chapters are devoted to basic knowledge of graphene, graphene FET and its biosensing. In the second part of this book the applications of graphene FET biosensors combined with various biotechnologies are presented. As well as discussing the existing technologies the authors also introduce their own ideas and concepts. Finally the remaining problems in graphene FET biosensors are discussed, along with proposed solutions and prospects for future applications. This monograph allows readers to grasp the basic knowledge and future direction of graphene-based FET biosensors.
Download or read book Immunocytochemical Methods and Protocols written by Constance Oliver and published by Humana Press. This book was released on 2012-04-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.
Download or read book Chemical and Synthetic Biology Approaches to Understand Cellular Functions Part C written by and published by Academic Press. This book was released on 2020-02-10 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more. Contains the authority of authors who are leaders in their field Provides a comprehensive source on new methods and research in enzymology
Download or read book Diagnostic Immunohistochemistry E Book written by David J Dabbs and published by Elsevier Health Sciences. This book was released on 2017-11-29 with total page 992 pages. Available in PDF, EPUB and Kindle. Book excerpt: User-friendly and concise, the new edition of this popular reference is your #1 guide for the appropriate use of immunohistochemical stains. Dr. David J. Dabbs and leading experts in the field use a consistent, organ system approach to cover all aspects of the field, with an emphasis on the role of genomics in diagnosis and theranostic applications that will better inform treatment options. Each well-written and well-researched chapter is enhanced with diagnostic algorithms, charts, tables, and superb, full-color histologic images, making this text a practical daily resource for all surgical pathologists. Features a systematic approach to the diagnostic entities of each organ system, including detailed differential diagnoses, diagnostic algorithms, and immunohistograms that depict immunostaining patterns of tumors. Covers many more antigens than other texts, and discusses antibody specifications with tables that convey information on uses, clones, vendors, sources, antibody titers, and types of antigen retrieval. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Contains new material on non-lymphoid malignancies, Hodgkin/non-Hodgkin lymphoma, and an expanded chapter on digital imaging and quantilative immunohistochemistry. Provides new grading schemes for several organs, along with new antibodies to cover more genomic immunohistochemistry applications. Offers more emphasis in the breast section of "eyes on" tissue for molecular/IHC prognostics compared to the current trend of gene-expression profiling of breast cancer.
Download or read book Biomolecular Patterning in Microfabricated Systems with Avidin biotin and Microcontact Printing written by Reid Nelson Orth and published by . This book was released on 2001 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immunohistochemistry and Immunocytochemistry written by Simon Renshaw and published by John Wiley & Sons. This book was released on 2017-02-06 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Download or read book Aptamers for Analytical Applications written by Yiyang Dong and published by John Wiley & Sons. This book was released on 2019-02-11 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.
Download or read book Immunohistochemistry in Diagnostic Dermatopathology written by Mai P. Hoang and published by Cambridge University Press. This book was released on 2017-09-28 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of only a few textbooks on the market dedicated to the important role of immunohistochemistry in diagnostic dermatopathology.
Download or read book Nutrition and Lifestyle for Pregnancy and Breastfeeding written by Peter D. Gluckman and published by . This book was released on 2015 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explaining the practical implications of new discoveries in 'life-course biology', Nutrition and Lifestyle for Pregnancy and Breastfeeding is an informed resource on factors that affect offspring development. The impact of parental lifestyle and behavioural choices influence not only fetal development and birth outcomes, but also postnatal development, yet guidance on appropriate diet, behaviour, and exposures during pregnancy is often confusing and contradictory. With accessible explanations of the latest scientific research, and clear summaries and recommendations, this book is a valuable and authoritative guide for all levels of health care providers. The authors provide an overview of the background evidence, highlighting the importance of lifestyle choices prior to and during pregnancy. In-depth discussions of nutritional and lifestyle factors that impact on pregnancy and offspring outcomes are based on the latest research and exploration of key scientific studies. Nutrition and Lifestyle for Pregnancy and Breastfeeding is a manual offering both scientific and clinical evidence to empower health care providers and ensure they have the information necessary to confidently care for prospective and new parents.
Download or read book Graphene Bioelectronics written by Ashutosh Tiwari and published by Elsevier. This book was released on 2017-11-22 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Graphene Bioelectronics covers the expending field of graphene biomaterials, a wide span of biotechnological breakthroughs, opportunities, possibilities and challenges. It is the first book that focuses entirely on graphene bioelectronics, covering the miniaturization of bioelectrode materials, bioelectrode interfaces, high-throughput biosensing platforms, and systemic approaches for the development of electrochemical biosensors and bioelectronics for biomedical and energy applications. The book also showcases key applications, including advanced security, forensics and environmental monitoring. Thus, the evolution of these scientific areas demands innovations in crosscutting disciplines, starting from fabrication to application. This book is an important reference resource for researchers and technologists in graphene bioelectronics—particularly those working in the area of harvest energy biotechnology—employing state-of-the-art bioelectrode materials techniques. Offers a comprehensive overview of state-of-art research on graphene bioelectronics and their potential applications Provides innovative fabrication strategies and utilization methodologies, which are frequently adopted in the graphene bioelectronics community Shows how graphene can be used to make more effective energy harvesting devices
