Download or read book Autophagy in Mammalian Systems Part B written by Daniel Klionsky and published by Academic Press. This book was released on 2009-03-05 with total page 571 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the companion volume to Daniel Klionsky's Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, "Autophagy: From phenomenology to molecular understanding in less than a decade. He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy.Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house cleaning in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.
Download or read book Autophagy Lower Eukaryotes and Non Mammalian Systems Part A written by and published by Academic Press. This book was released on 2011-09-02 with total page 821 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the companion volume to Daniel Klionsky's Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi. Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, "Autophagy: from phenomenology to molecular understanding in less than a decade. He is currently editing guidelines for the field, with 230 contributing authors that will publish in Autophagy.Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house-cleaning in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 in yeast. These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth. - Establishes the functional roles of specific cellular proteins in selective and nonselective autophagy in mammalian cells, which aides researchers in determining why autophagy is shut down in neoplastia (growth of abnormal tissue mass) and turned on during bacterial invasion - Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture, which helps researchers design clinical approaches that can turn on autophagy and halt tumor growth - Covers higher eukaryotes including lifespan in C.elegans to marine organisms and bridging into the clinical aspects, including autophagy in chronic myelogenous leukemia (CML is one of four types of leukemia), lung cancer, prostate cancer, and cardiac cells
Download or read book Autophagy written by Daniel J. Klionsky and published by . This book was released on 2008 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Autophagy written by Klionsky, Daniel J. and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the companion volume to Daniel Klionsky's Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, "Autophagy: from phenomenology to molecular understanding in less than a decade." He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house-cleaning" in the cell is called autophagy - literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth. *Establishes the functional roles of specific cellular proteins in selective and nonselective autophagy in mammalian cells which aids researchers in determining why autophagy is shut down in neoplastia (growth of abnormal tissue mass) and turned on during bacterial invasion. *Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture, which helps researchers design clinical approaches that can turn on autophagy and halt tumor growth. *Covers higher eukaryotes including lifespan in C. elegans to marine organisims and bridging into the clinical aspects, including autophagy in chronic myelogenous leukemia (CML is one of four types of leukaemia), lung cancer, prostate cancer, and cardiac cells
Download or read book Autophagy in Disease and Clinical Applications Part C written by Daniel Klionsky and published by Academic Press. This book was released on 2009-02-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third and final installment of Daniel J. Klionsky's new three-volume treatment of autophagy, this volume focuses on monitoring autophagy with regard to disease connections, and presents methods that can be used to analyze autophagy in clinical samples. Edited by one of the leading authorities in the field, this volume and its companion volumes, Autophagy: Lower Eukaryotes and Autophagy in Mammalian Systems, provide a comprehensive overview of the techniques involved in studying autophagy in eukaryotes and simple animal systems, mammalian cells and non-human animals, and humans. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house cleaning” in the cell is called autophagy - literally self-eating. In the future, research in this field will help to design clinical approaches that can turn on autophagy and halt tumor growth.
Download or read book Autophagy written by Kelsey B. Law and published by Elsevier Inc. Chapters. This book was released on 2013-09-03 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt: Macroautophagy is mainly considered to be a mechanism for the bulk degradation of the cytoplasm in times of nutrient depletion. However, growing evidence suggests that macroautophagy is more substrate-specific than originally considered. Numerous cytosolic components are selectively degraded by macroautophagy, including aggresomes, damaged mitochondria, peroxisomes, ribosomes, midbodies, and bacteria and viruses. Although the specific molecular components may differ for each substrate, the general mechanism of selective macroautophagy involves the targeted ubiquitination of the substrate and the recruitment of autophagy receptors. Autophagy receptors are proteins that act as an interface between the substrate and the nascent autophagosome, the double-membrane structure that sequesters the cytoplasm for delivery to lysosomes for degradation. In this chapter we will describe the general mechanism of selective autophagy in the mammalian system, focusing on the most described autophagy receptor, p62. The emerging data suggest that selective autophagy is not only necessary for cell survival during nutrient starvation, but also plays a critical role in cell development, cellular responses to oxidative stress, and innate immunity.
Download or read book Molecular Characterization of Autophagic Responses Part B written by and published by Academic Press. This book was released on 2017-02-22 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional. - Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses - Written in an accessible style by renowned experts in the field
Download or read book Synthetic Biology Part B written by Chris Voigt and published by Academic Press. This book was released on 2011-07-15 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation
Download or read book Biothermodynamics written by Michael L. Johnson and published by Academic Press. This book was released on 2011 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last several years there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.
Download or read book Two Component Signaling Systems Part C written by and published by Academic Press. This book was released on 2010-03-01 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with the Sln-1 kinase pathway, triazole sensitivity in C. albicans, and histidine kinases in cyanobacteria circadian clock. - Includes time-tested core methods and new innovations applicable to any researcher studing two-component signaling systems or histidine kinases - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Download or read book Computer Methods Part B written by and published by Academic Press. This book was released on 2009-11-05 with total page 711 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with previous and forthcoming Computer Methods volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research. - Presents step-by-step computer methods and discusses the techniques in detail to enable their implementation in solving a wide range of problems - Informs biomedical researchers of the modern data analysis methods that have developed alongside computer hardware - Presents methods at the "nuts and bolts" level to identify and resolve a problem and analyze what the results mean
Download or read book Protein Engineering for Therapeutics Part B written by and published by Academic Press. This book was released on 2012-01-25 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds - Chapters provide an invaluable resource for academics, researchers and students alike - Iinternational board of authors - This volume is split into sections that cover subjects such as peptides, and scaffolds
Download or read book Methods in Methane Metabolism Part B written by Amy Rosenzweig and published by Academic Press. This book was released on 2011-03-17 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. - Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements - The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field
Download or read book Cryo EM Part B 3 D Reconstruction written by and published by Academic Press. This book was released on 2010-09-30 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
Download or read book Guide to Techniques in Mouse Development Part B written by Paul Wassarman and published by Academic Press. This book was released on 2010-09-24 with total page 637 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
Download or read book Thiol Redox Transitions in Cell Signaling Part B written by and published by Academic Press. This book was released on 2010-08-19 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. - Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling - Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Download or read book Mitochondrial Function Part B written by and published by Academic Press. This book was released on 2009-05-05 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this second of two new volumes covering mitochondria, methods developed to assess the number and function of nuclear-encoded proteins in the mitochondrion are presented. Chapters focus on the regulation of mitochondrial function and mitochondrial diseases, with a section emphasizing the mitochondrial defects associated with type 2 diabetes. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. With more than 450 volumes published, each volume presents material that is relevant in today's labs, truly an essential publication for researchers in all fields of life sciences. - New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them - Along with a companion volume covering topics including mitochondrial electron transport chain complexes and reactive oxygen species, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction - Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods
