Download or read book Aqueous Size Exclusion Chromatography written by P.L. Dubin and published by Elsevier. This book was released on 1988-05-01 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole.This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.

Download or read book Aqueous Size exclusion Chromatography written by Paul L. Dubin and published by . This book was released on 1988 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Aqueous Size exclusion Chromatography written by Paul Dubin and published by . This book was released on 1988 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Size Exclusion Chromatography written by Sadao Mori and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

Download or read book Size Exclusion Chromatography written by B. J. Hunt and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a large and increasing variety of polymers currently in use both for domestic and industrial applications. The properties of polymers are deter mined not only by their chemical type, but also by their molecular mass and molecular mass distributions. However, while the chemical type of polymers can be determined relatively easily, the average molecular masses and molecular mass distributions are more difficult to measure. The molecular mass averages of a polymer are measured by specialized and complex techniques such as light scattering (for weight average) and osmometry (for number average). Thus, complete characterization of the molecular mass distribution of a polymer by such means requires separating the sample into many fractions which can then be examined individually. Since size exclusion chromatography was introduced as a rapid and straightforward technique for the characterization of polymer molecular mass distributions, there have been tremendous increases in development and applications, and it was felt appropriate to bring together into a single volume the information required by scientists from many disciplines who wish to use the technique. This book should be useful to existing users, those who are new to the technique, and those who may be familiar with the basic technique and now wish to extend their capabilities to more complex applications (or to consider the potential of a number of related techniques). The book will also be of general interest to the experienced liquid chromatographer.

Download or read book Ion Exchange Chromatography of Proteins written by Shuichi Yamamoto and published by CRC Press. This book was released on 1988-04-14 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.

Download or read book Modern Size Exclusion Liquid Chromatography written by Andre Striegel and published by John Wiley & Sons. This book was released on 2009-07-31 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Download or read book Ideal Aqueous Size Exclusion Chromatography written by Mamta Suresh Mehta and published by . This book was released on 1991 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Column Handbook for Size Exclusion Chromatography written by Chi-san Wu and published by Academic Press. This book was released on 1999 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: The column is the heart of a chromatographic system. For the first time, complete and authoritative information on columns for size exclusion and gel filtration chromatography is available in a single volume containing contributions from every major manufacturer worldwide and many renowned column users. This comprehensive handbook covers the technology, characterization, application, evaluation, maintenance, and quality control of commercial analytical and preparative columns for SEC, GFC, and two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Experienced column users discuss various applications in organic and aqueous mobile phases for synthetic and biopolymers, small solutes, biopharmaceutical therapeutics, and high-temperature SEC.

Download or read book Aqueous Size Exclusion Chromatography of Non derivatized Cellulose written by and published by . This book was released on 1982 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Aqueous Size Exclusion Chromatography of Non derivatized Cellulose written by Arindam Bose and published by . This book was released on 1980 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Practice of High Performance Liquid Chromatography written by Heinz Engelhardt and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Download or read book Handbook Of Size Exclusion Chromatography And Related Techniques written by Chi-San Wu and published by CRC Press. This book was released on 2003-11-04 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

Download or read book Method Optimization of Aqueous Size Exclusion Chromatography for Six Proteins a Study of Lysozyme Ovalbumin Bovine Serum Albumin Human Serum Albumin Immunoglobulin and Thyroglobulin by Size Exclusion Chromatography Including Degradation Studies written by Elizabeth Davidson and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "High-performance liquid chromatography (HPLC) is used often for the separation of proteins or other biomolecules. One type of HPLC used to determine the purity of a protein is size-exclusion chromatography (SEC). The size-based separation in SEC occurs when smaller molecules are retained in the pores of the stationary phase, while larger molecules are eluted first. This allows aggregates of proteins as well as any small contaminants to be clearly separated. SEC can be used either with aqueous or organic mobile phase. In this study, six different proteins were studied using aqueous SEC: bovine serum albumin, human serum albumin, immunoglobulin, lysozyme, ovalbumin, and thyroglobulin. Mobile phase conditions were optimized for separating the monomer from the aggregates of each of these compounds. A calibration curve was constructed using the monomer of each of the proteins. The proteins were then degraded under different conditions, including acid, base, oxidation, and UV light to study the molecular weight effects of degradation on proteins. The method was then evaluated using various validation studies. It was found that separating similarly sized proteins is very difficult in SEC, but that the aggregates of each protein can be separated well from the monomer."--

Download or read book Column Handbook for Size Exclusion Chromatography written by Chi-san Wu and published by Elsevier. This book was released on 1999-03-19 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Key Features* Evaluate and select columns with confidence for specific applications* Optimize separations and improve the ruggedness of analytical methods* Extend the service time of a column* Establish a quality-control program to ensure consistency in column performance* Avoid the expense of column damage or purchases that do not give the expected results

Download or read book Handbook Of Size Exclusion Chromatography And Related Techniques written by Chi-San Wu and published by CRC Press. This book was released on 2003-11-04 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

Download or read book Introduction to Microscale High performance Liquid Chromatography written by Daidō Ishii and published by . This book was released on 1988 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: