Download or read book Applications of UV Resonance Raman and Micro Raman Spectroscopy for the Rapid Characterization of Bacteria and the Study of Polymers written by Suneet Chadha and published by . This book was released on 1993 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rapid Characterization of Microorganisms by Uv resonance Raman and Fluorescence Spectroscopy written by Mi-Ok P. Baek and published by . This book was released on 1988 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Basic Study to Assess the Potential Usefulness of Resonance Raman Spectroscopy as a Means of Rapidly Detecting and Identifying Bacteria and Other Microorganisms written by W. H. Nelson and published by . This book was released on 1985 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: This past year we have explored the potential uses of Resonance Raman and Fluorescence Lifetime Spectroscopies in the rapid characterization of bacteria. Previously we have shown that chromobacteria can be distinguished on the basis of distinctive resonance Raman spectra. Spectra are excited by low power argon ion laser radiation at 488 nm and are due to the presence of carotenoid pigments. While carotenoid pigments in bacteria generally are not useful for identification purposes, the study of chromobacteria has allowed us to assess the potential sensitivity of resonance Raman spectroscopy in this application. We were able to laser illuminate and count two types of bacteria under a microscope. Because it was possible to see the laser excited bacteria while the resonance Raman spectra were being obtained, it was possible to estimate closely the number of bacteria producing a given spectrum. Such high sensitivity suggests but does not prove that spectra are 'surface enhanced'. It is clear that the resonance Raman technique can be highly sensitive and our experiments suggest that remote detection and detection from mixtures is possible in principle.
Download or read book Micro Raman Spectroscopy written by Jürgen Popp and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-02-10 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro-Raman Spectroscopy introduces readers to the theory and application of Raman microscopy. Raman microscopy is used to study the chemical signature of samples with little preperation in a non-destructive manner. An easy to use technique with ever increasing technological advances, Micro-Raman has significant application for researchers in the fields of materials science, medicine, pharmaceuticals, and chemistry.
Download or read book The Rapid Detection of Bacteria and Other Microorganisms A Basic Study in the Application of Resonance Raman and Time Resolved Fluorescence Spectroscopies written by Wilfred H. Nelson and published by . This book was released on 1988 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: Resonance Raman Spectra have been obtained for a variety of chromobacteria using low power 488 nm excitation. Spectra are simple, of high quality, and useful for identification purposes at the species level. Raman microprobe studies show conclusively that spectra can be obtained from single cells in pure cultures or in mixed cultures without need for separation. Extensions of the study have been made to representative colorless gram-negative and gram-positive bacteria. Spora other than bacteria have been studied as well. Pollen, mold spores, bacterial spores, algae and viruses all give spectra but only viruses and bacterial spores appear to give intense UV Resonance Raman spectra. The primary fluorescence of bacteria has been studied in detail to determined its potential in rapid detection. We have determined fluorescence for S. epidermidis, P. fluorescens, E. cloacae, E. coli and B. subtilis. Fluorescence contributions have been assigned in part to tryptophan, pteridines, related flavins and pyridine coenzymes.
Download or read book Applications of Infrared Raman and Resonance Raman Spectroscopy in Biochemistry written by Frank S. Parker and published by Springer Science & Business Media. This book was released on 1983-10-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Raman Spectroscopy to Biology written by M. Ghomi and published by IOS Press. This book was released on 2012-02-23 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 1006 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Raman Spectroscopy written by Gustavo Morari Do Nascimento and published by BoD – Books on Demand. This book was released on 2018-04-18 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a wide overview of the state-of-the-art applications of Raman spectroscopy in characterization of materials and biomaterials. The Raman signal is intrinsically smaller than other vibrational techniques; however, mainly through intensification processes, such as resonance Raman (RR) and surface-enhanced Raman spectroscopy (SERS), the Raman cross section can be strongly amplified. Thoroughly in these signal amplifications, the study of a diversity of chemical systems and the use of Raman technique for in situ and in vivo measurements is possible. The main goal of this book is to open up to an extended audience the possibilities of uses of Raman spectroscopy. In fact, this collective work will be beneficial to students, teachers, and researchers of many areas who are interested to expand their knowledge about Raman spectroscopy applied to nanotechnology, biotechnology, environmental science, inorganic chemistry, and health sciences.
Download or read book The Rapid Detection of Single Bacterial Cells by Deep UV Micro Raman Spectroscopy written by W. H. Nelson and published by . This book was released on 1992 with total page 38 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Download or read book Biochemical Applications of Raman and Resonance Raman Spectroscopes written by P Carey and published by Elsevier. This book was released on 2012-12-02 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.
Download or read book Raman Spectroscopy for Nanomaterials Characterization written by Challa S.S.R. Kumar and published by Springer Science & Business Media. This book was released on 2012-03-30 with total page 659 pages. Available in PDF, EPUB and Kindle. Book excerpt: First volume of a 40-volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about Raman spectroscopy for the characterization of nanomaterials. Modern applications and state-of-the-art techniques are covered and make this volume essential reading for research scientists in academia and industry.
Download or read book Application of Molecular Methods and Raman Microscopy Spectroscopy in Agricultural Sciences and Food Technology written by Biljana Vucelić-Radović and published by Ubiquity Press. This book was released on 2019-07-23 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: his book has been prepared with the aim to present the application of these two state-of-the art technologies in agricultural sciences and food technology, and to explain the protocols for analyses of different plant, animal, microbiological and food samples as well as for different biotechnology procedures. Selected methods and protocols which are used in plant stress physiology, weed science, fruit breeding research, microbial ecology, plant virus and fungus diagnostics, phytobacteriology, fishery, food biochemistry, food materials and food technology are described. Special adaptation of certain protocols is required for application in each of these sciences, for every type of GMO organism, food technology raw material, and food technology product, as well as for every type of bacteria, virus, fungus or fungus-like organism, for each type of raw material in terms of plant host species, plant organs, year period and conditions in the laboratory. Application of molecular methods, primarily qPCR, and Raman microscopy/ spectroscopy in agricultural and food sciences provides substantial opportunity for increased production efficiency, food safety, better product quality and improvement of plant and animal health. This book is aimed for students, scientists and professionals working in the field of agriculture and food technology.
Download or read book Raman Imaging written by Arnaud Zoubir and published by Springer. This book was released on 2012-07-27 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman imaging has long been used to probe the chemical nature of a sample, providing information on molecular orientation, symmetry and structure with sub-micron spatial resolution. Recent technical developments have pushed the limits of micro-Raman microscopy, enabling the acquisition of Raman spectra with unprecedented speed, and opening a pathway to fast chemical imaging for many applications from material science and semiconductors to pharmaceutical drug development and cell biology, and even art and forensic science. The promise of tip-enhanced raman spectroscopy (TERS) and near-field techniques is pushing the envelope even further by breaking the limit of diffraction and enabling nano-Raman microscopy.
Download or read book Application of Raman Spectroscopy for Studying Bacteria of the Family Enterobacteriaceae written by Amir Nakar and published by . This book was released on 2023* with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis investigated using Raman spectroscopy for identifying Enterobacteriaceae and their antibiotic resistances. The study aimed to develop a rapid, accurate diagnostic tool, as current methods are costly and inefficient, leading to increased antibiotic resistance due to inappropriate antibiotic use. The research involved collecting a dataset of Enterobacteriaceae, encompassing 8 genera. Measurements were taken using Single-Cell Raman Microspectroscopy (SC-RMS) and Fiber Probe-Based Raman Spectroscopy (FP-RS). Machine learning models were trained to classify the bacteria based on spectral data. SC-RMS showed limited success (47% accuracy), while FP-RS achieved 100% accuracy on the training dataset and 94% on a validation dataset, demonstrating its potential in differentiating Enterobacteriaceae. Subsequently, Raman spectra of 24 clinical isolates were analyzed using UV-Resonance Raman Spectroscopy (UVRRS). The goal was to discriminate between E. coli, K. pneumoniae, and K. oxytoca. While SC-RMS had limited effectiveness, UVRRS achieved high accuracy (92% at the genus level and 90% at the species level), marking the first successful differentiation of K. oxytoca using Raman spectroscopy. The study also explored differentiating antibiotic-resistant and sensitive Enterobacteriaceae. Both SC-RMS and UVRRS correctly classified 70-75% of the isolates, with UVRRS demonstrating a 90% sensitivity for resistant strains. This differentiation may be related to a higher DNA/Protein ratio in resistant bacteria. Overall, the findings enhance the application of Raman spectroscopy in microbiology, particularly for Enterobacteriaceae. The research supports its use in identifying pathogens, determining their genus and species, and assessing antibiotic resistance. The study contributes significantly to the development of Raman-based microbial diagnostics, promising to improve infectious disease diagnostics, patient care, and the fight against antimicrobial resistance.
Download or read book Infrared and Raman Spectroscopy written by Günter G. Hoffmann and published by Walter de Gruyter GmbH & Co KG. This book was released on 2023-09-18 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quite a few excellent books about vibrational spectroscopy have already been published. So why write a new one? The last years have seen the birth of new techniques and, first of all, a wealth of new applications. Therefore, a lot of new users need an introduction to these techniques and applications, but, if they are new to vibrational spectroscopy, an introduction to the parent techniques as well. Vibrational spectroscopies can detect and analyze vibrations in molecules. Mainly two different forms are used today: Infrared and Raman spectroscopy. Vibrational spectroscopy is used by chemists to characterize their substances. If the spectra of substances are known, analytical chemists can use them to analyze a mixture of chemicals. Samples may be analyzed even with spatial resolution, on the microscopic as well as on the macroscopic scale. "Infrared and Raman Spectroscopy" is intended for researchers or lecturers in Chemistry, Physics, Materials Science and Life Sciences, who are interested in the composition and properties of their samples. It describes how vibrational spectroscopy will enable them to examine thin layers, surfaces and interfaces, and also improve their knowledge about the properties of composites. Special chapters introduce VCD, ROA, and TERS. The book can serve as a short introduction to vibrational spectroscopy too, so that students at the first graduate level will benefit from it as well.
