Download or read book Applications of Surface enhanced Raman Spectroscopy for the Study of Indicators of Exposure to Selected Genotoxins written by Anne Marie Helmenstine and published by . This book was released on 1992 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface Enhanced Raman Spectroscopy written by Marek Prochazka and published by Springer. This book was released on 2015-12-12 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1993 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Surface Enhanced Raman Spectroscopy written by Stuart Farquharson and published by CRC Press. This book was released on 2016-11-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: With comprehensive coverage, Applications of Surface-Enhanced Raman Spectroscopy provides practitioners of SERS with a resource of current applications described by experts in each area to guide their own applications of this technology. This book covers both the traditional applications in chemical and biochemical research as well as commercial applications in the areas of environmental science, forensics, and pharmaceuticals and biotechnology. It emphasizes the hot-topic areas of homeland security and medical applications. Offering groundwork for future application development, this text is essential for analytical chemists involved in trace chemical analysis and those companies selling SERS products.
Download or read book Applications of Raman Spectroscopy to Biology written by M. Ghomi and published by IOS Press. This book was released on 2012-02-23 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.
Download or read book Surface enhanced Raman Spectroscopy written by Nicolae Leopold and published by . This book was released on 2009 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1992 with total page 796 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Application of Surface Enhanced Raman Spectroscopy to the In Situ Study of Passivity and Inhibition written by Lucy Jane Oblonsky and published by . This book was released on 1993 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface enhanced Raman Scattering Based Immunoassay Platforms for Viral Pathogen Detection written by Jing Neng and published by . This book was released on 2012 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface-enhanced Raman scattering (SERS) based biosensors have been widely applied in biomedical diagnostics for its robustness, high sensitivity, improved levels of multiplexing and potential to perform detection in a point-of-care platform. Recent progress in SERS has been demonstrated for a wide range of biosensor applications, ranging from nucleic acid and immunoassay detection to tumor imaging and cancer diagnosis. SERS-based immunoassays have gained considerable attention for target pathogen detection in food safety and public health assurance. In this work, we have employed different versions of this strategy in the low-level detection of proteins (e.g., immunoglobulins and viral antigens). Therefore, we can detect either the exposure to the pathogen or the existence of pathogen. First, a novel SERS immunoassay for detection of exposure to viral antigen has been developed which used antigen-coated gold nanoparticles (GNPs) for antibody capture and Raman label-conjugated protein A/G (pA/G) as a bi-functional reporter. GNPs were first coated with a recombinant His + -tagged fusion of the West Nile virus (WNV) envelope (E) protein and then incubated with rabbit antiserum raised against recombinant E protein and finally mixed with malachite green (MG)-labeled protein A/G (p/AG). Laser interrogation of the GNP/WNV E antibody/MG-pA/G immunocomplex in solution provided an intense characteristic Raman spectrum of MG which indicated the presence of WNV E antiserum and provided a limit of detection (LOD) of ~2 ng/ml total serum protein. Compared to enzyme-linked immunosorbent assay (ELISA) that were performed using the same antigen/antibody reagents, the LOD for the SERS immunoassay was found to be >400-fold more sensitive. In the next study, we proposed a cost-effective, highly sensitive and reproducible magnetic nanoparticle (MNP) capture-based SERS assay for the detection of two viral antigens. GNPs were conjugated with the capture antibodies and the corresponding Raman reporters, followed by hybridizing with the West Nile virus antigen (WNV) and the Rift Valley fever virus (RVFV) antigen as well as the capture MNPs conjugated with specific antibodies. Using an external magnet source, two different hybridization complexes, Infrared-792 (IR-792) labeled GNP/WNV antigen/MNP and Nile Blue (NB) labeled GNP/RVFV antigen/MNP, were removed from solution followed by laser interrogation of the compacted pellet. Laser excitation of the pelleted material provided a mixed spectrum representing the combination of IR-792 and NB signals obtained from each SERS probes, which is diagnostic for recognition of both antigens. The LOD was ~5 fg/ml for assays conducted in phosphate buffered saline buffer (PBS) and ~25 pg/ml for assays containing PBS spiked with fetal bovine serum. Finally, we demonstrated a robust SERS-based immunoassay for the simultaneous detection of three viral antigens spiked in serum through magnetic capture of the silica-encapsulated SERS nanotags. The specific capture of all the antigens in individual assay reactions was performed by incubation with mixtures of silica-coated nanotags (SiNTGs) and magnetic nanoparticles (MNPs), both conjugated with polyclonal antibodies specific for antigen recognition. The SiNTGs provided distinct spectra that correspond to each antigen capture and were impervious to matrix effects. Detection was provided by magnetic pull-down of the three different SiNTG/antigen/MNP immune-recognition complexes and laser excitation of the three spectrally distinct Raman labels. The LOD was ~ 0.01 ng/ml in 20% fetal bovine serum (FBS) for three antigens, a significant improvement over previous studies in terms of the sensitivity, level of multiplexing and medium complexity.
Download or read book Use of Surface enhanced Raman Spectroscopy in the Study of the Adsorption Behaviors of Corrosion Inhibitors on Metal Surfaces written by Albert Thomas Fakhri El-Hage and published by . This book was released on 1985 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Exploring the Challenges of Advancing Surface Enhanced Raman Spectroscopy Based Biosensing From Research Laboratory to Clinics written by Owen Suyuan Liang and published by . This book was released on 2020 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt: Healthcare has seen a big boon in technological advancement with the last few years with next-generation genome sequencing, robotics, artificial intelligence, and bioinformatics. While the origins of these breakthroughs are deeply rooted in biology, life sciences, and medical science, the new age of technological breakthrough require an interdisciplinary approach. Materials science itself is an interdisciplinary field, with a huge contribution of physics and chemistry, and within the past few decades, advances in nanotechnology has allowed integration into healthcare giving materials science a new emerging role. Such is the case with a vibrational spectroscopy technique called Surface Enhanced Raman Spectroscopy (SERS). SERS has the ability to measure the molecular bonds of the target analyte at the single molecular level. Yet, SERS is not a recognized spectrometry tool in healthcare. This dissertation examines the fundamental challenges that require a technique like SERS to transition from the academic research laboratories to clinical applications. While there are broad potential clinical applications, this dissertation focuses on the SERS measurement of diseases originating from cellular morphologies. The two main focal topics are cancer and microbiology research, where the proteome identification can provide new diagnostic and therapeutic treatment options. The first major section is about testing the capabilities of our group's unique graphene gold nanopyramid hybrid platform in controlled research laboratory environments. Varies cases of cancers are examined: lung, skin, and breast, through their various size, morphology, and proteomic differences. Afterwards, the field of microbiology is explored with bacteria and fungus species. The second major section involves the SERS's platform performance with clinical patient data. The first case study looks at the diagnosing meningitis from patients in China. The second case study looks at multidrug resistance detection of tuberculosis in rural Pakistan. The last major section tries to consolidate the major lessons in this journey of bringing SERS to clinical applications. In particular, this section tackles the main question of why a technique like SERS has not been fully recognized in clinics yet and brings the case to why our SERS hybrid platform is very close to it.
Download or read book Food Safety 1995 written by Carol E. Steinhart and published by CRC Press. This book was released on 1995-06-06 with total page 784 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering recent developments in food safety and foodborne illnesses, this work organizes information to provide easy access to general and specific topics. It offers comprehensive summaries of advances in food science, compiled from over 620 sources worldwide. The main focus is on health and safety, with extensive reviews of microbiological and medical subjects.
Download or read book Application of Surface Enhanced Raman Spectroscopy to Biological Systems written by and published by . This book was released on 2013 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Raman Spectroscopy and Instrumentation for Monitoring Soil Carbon Systems written by D. L. Stokes and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This work describes developments in the application of Raman scattering and surface-enhanced Raman scattering (SERS) towards the assessment/characterization of carbon in soil. In the past, the nonspecific total carbon mass content of soil samples has generally been determined through mass loss techniques and elemental analysis. However, because of the concern over CO{sub 2} buildup in the atmosphere and its possible role in the ''Greenhouse Effect, '' there is a need for better-defined models of global cycling of carbon. As a means towards this end, there is a need to know more about the structure and functionality of organic materials in soil. Raman spectroscopy may therefore prove to be an exceptional tool in soil carbon analysis. Based on vibrational transitions of irradiated molecules, it provides structural information that is often suitable for sample identification. Furthermore, Raman scattering yields very fine spectral features which offer the potential for multicomponent sample analysis with minimal or no sample pretreatment. Although the intensity of Raman scattering is generally extremely low, the surface-enhanced Raman scattering (SERS) effect can greatly enhance Raman signals (10{sup 6}-10{sup 8} range) through the adsorption of compounds on specially roughened metal surfaces. In our laboratory, we have investigated copper, gold and silver as possible substrate metals in the fabrication of SERS substrates. These substrates have included metal-coated microparticles, metal island films, and redox-roughened metal foils. We have evaluated several laser excitation sources spanning the 515-785 nm range for both Raman and SERS analysis. For this particular study, we have selected fulvic and humic acids as models for establishing the feasibility of using Raman and SERS in soil carbon analysis. Our studies thus far have demonstrated that copper substrates perform best in the SERS detection of humic and fulvic acids, particularly when coupled to electrochemical processes that enhance adsorption of specific compounds. This effect not only yields a stronger signal, but can also impart selectivity in the analysis of complex samples such as soil.
Download or read book Surface Enhanced Raman Spectroscopy SERS Based on ZnO Nanorods for Biological Applications written by Jun Ki Kim and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detection of nanometer-sized biomarkers is a research topic that attracts much attention as an application for early diagnosis of diseases. Biopsy monitoring by analyzing cell secretion in a non-destructive way has many advantages in the field of biomedicine. We introduce the Raman signal enhancement method on a biosensing chip based on surface-enhanced Raman diagnosis. This approach has the advantage because the ZnO nanorods are grown to form nanoscale porosity and are coated with gold to enable size selective biomarker detection. After sputtering gold on the grown ZnO nanostructures, the unique feature of clustering the nanorod,Äôs heads first appeared. The grain formation on the head was the main factor for the localized surface plasmon resonance (LSPR) enhancement, and this fact could be verified by finite element analysis. It has been demonstrated in breast cancer cell line that the cell viability is also high in such gold-clad ZnO nanostructure-based surface-enhanced substrates. For bioapplication, interstitial cystitis/bladder pain syndrome (IC/BPS) animal model was prepared by injecting HCl into the bladder of a rat, and urine was collected a week later to conduct Raman spectroscopy experiments.
Download or read book Application of Surface Enhanced Raman Spectroscopy and Multivariate Evaluation Techniques on Biological Samples written by Charlotte Eliasson and published by . This book was released on 2001 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Surface Enhanced Raman Spectroscopy on Nanoscale Ultrathin Films Prepared by Atomic Layer Deposition written by 粘立暐 and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
