Download or read book Application of a New Fluorescence Resonance Energy Transfer FRET System in Synthetic DNA written by Lilia Clima and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Theoretical Studies of Dye labeled DNA Systems with Applications to Fluorescence Resonance Energy Transfer written by Elena Dolghih and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this work we describe a molecular dynamics study of several dye-DNA systems in which we examine how fluorescent probes interact with DNA and the effects of these interactions on FRET. Structural fluctuations in interchromophore distances and dipole orientations of the probes, as well as rates and efficiencies of energy transfer are evaluated carefully in the light of Förster-proposed approximations. Theoretical results are compared to available experimental data and possible improvements in system design with respect to the location and the mode of attachment of the probes are proposed.
Download or read book Frontiers in Nano and Micro Device Design for Applied Nanophotonics Biophotonics and Nanomedicine written by A. Guillermo Bracamonte and published by Bentham Science Publishers. This book was released on 2021-07-30 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference informs readers about nanoscale design and synthesis of different nanomaterials. Chapters of the book account for variable nanoarchitecture, while explaining concepts which are central to the field of nanotechnology. It explains how nanodevices and microdevices can be used for nanophotonics, biophotonics and drug delivery applications. Advanced biochemical techniques ranging from fluorescence, plasmonics, enhanced plasmonics (EP) to metal enhanced fluorescence (MEF) from colloidal dispersion to single luminescent nanoplatforms and nanospectroscopy, microfluidics, nanofluidics, silica wave-guiding, lasers, nanolasers and photonic circuits for enhanced signal detections are also presented. In addition, proof of concept ideas of microdevices and nanodevices to real applications within other allied disciplines such as genomics, biochemistry, drug delivery and clinical chemistry (based on advanced optical detection and imaging) are highlighted. The book is an informative reference for readers studying biochemistry, pharmacology, biomedical engineering and related subjects at all levels, as well as general readers who want to learn about advanced applications in optics and photonics.
Download or read book Artificial DNA written by Yury E. Khudyakov and published by CRC Press. This book was released on 2002-09-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening fo
Download or read book New Frontiers and Applications of Synthetic Biology written by Vijai Singh and published by Academic Press. This book was released on 2022-01-12 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Frontiers and Applications of Synthetic Biology presents a collection of chapters from eminent synthetic biologists across the globe who have established experience and expertise working with synthetic biology. This book offers several important areas of synthetic biology which allow us to read and understand easily. It covers the introduction of synthetic biology and design of promoter, new DNA synthesis and sequencing technology, genome assembly, minimal cells, small synthetic RNA, directed evolution, protein engineering, computational tools, de novo synthesis, phage engineering, a sensor for microorganisms, next-generation diagnostic tools, CRISPR-Cas systems, and more. This book is a good source for not only researchers in designing synthetic biology, but also for researchers, students, synthetic biologists, metabolic engineers, genome engineers, clinicians, industrialists, stakeholders and policymakers interested in harnessing the potential of synthetic biology in many areas. - Offers basic understanding and knowledge in several aspects of synthetic biology - Covers state-of-the-art tools and technologies of synthetic biology, including promoter design, DNA synthesis, DNA sequencing, genome design, directed evolution, protein engineering, computational tools, phage design, CRISPR-Cas systems, and more - Discusses the applications of synthetic biology for smart drugs, vaccines, therapeutics, drug discovery, self-assembled materials, cell free systems, microfluidics, and more
Download or read book Single molecule and Ensemble Studies of DNA Replication System written by Cheng Liu and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA replication is one the most important and complex systems in biology. In this dissertation, we first started from ensemble experiments, studying the clamp (PCNA) and the clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans, and then moved to the single-molecule level trying to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a real-time fluorescence assay, we discovered that RFC can assemble a PCNA ring from monomers in solution. A motion-based DNA polymerization assay showed that the PCNA assembled by RFC is functional. This PCNA assembly activity required the ATP-bound conformation of RFC. Our work demonstrated a reverse-chaperoning activity for an AAA+ protein that can act as a template for the assembly of another protein complex. In the second study, by applying fluorescence resonance energy transfer (FRET) to the archaeal replication, we reconstituted part of replication machine, up to four different protein components, at the single-molecule level. We developed a surface-based assay where the loading of the PCNA to the DNA by the RFC was visualized in real time. We discovered an intermediate step likely due to ATP hydrolysis by RFC before PCNA was released on the DNA. Although PCNA itself was not stable as a trimeric ring, once loaded, PCNA remained stably associated with the DNA for hours, allowing us to investigate the subsequent reactions. We found that PCNA prefers to stay near the primer/template junction but still diffuses on both double and single stranded DNA. This is only the second example of direct observation of protein diffusion on single stranded DNA. Diffusion on the single strand, however, is two orders of magnitude slower than on double stranded DNA, and is prevented by cognate single strand binding protein. By adding the DNA polymerase to the loaded clamp, we could follow DNA synthesis by the polymerase-clamp complex by visualizing the motion of the clamp downstream. Interestingly, PCNA frequently slipped back or paused during synthesis, suggesting that spontaneous diffusion of PCNA or its complex with the polymerase is an integral feature even during polymerization. In the last chapter and appendices, some additional works regarding new instrument development and statistical algorithms for programming are also included.
Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Humana. This book was released on 2006-04-01 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.
Download or read book FRET F rster Resonance Energy Transfer written by Igor L. Medintz and published by John Wiley & Sons. This book was released on 2013-10-17 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.
Download or read book Mobility and Proximity in Biological Membranes written by S. Damjanovich and published by CRC Press. This book was released on 2018-01-18 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell surface membranes have long been characterized as two-dimensional fluids whose mobile components are randomized by diffusion in the plane of the membrane bilayer. Recent research has indicated that cell surface membranes are highly organized and ordered and that important functional units of membranes appear as arrays of interacting molecules rather than as single, freely diffusing molecules. Mobility and Proximity in Biological Membranes provides an overview of the results obtained from biophysical methods for probing the organization of cell surface membranes. These results are presented in the context of detailed treatments of the theory and the technical demands of each of the methods. The book describes a versatile and easily applied mode for investigating molecular proximities in plasma membranes in a flow cytometer. Its analysis of lipid fluidity and viscosity of membranes and the rotational mobility of proteins offers intimate insight into the physical chemistry of biological membranes. The electrophysiology of lymphocytes is presented with focus on its importance in different diseases. New techniques are described, and new data, new possibilities, and future trends are presented by world experts. This book's chapters can serve both as guides to the existing literature and as starting points for new experiments and approaches associated with problems in membrane function.
Download or read book Fluorescence Resonance Energy Transfer FRET Based Sensors for Bioanalysis written by Gabriela Blagoi and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of my PhD study was to develop and characterize new methods and sensors based on fluorescence resonance energy transfer (FRET) for bioanalysis. Chapter 3 describes the use of FRET between donor fluorophores and acceptor labeled murine macrophage cells. FRET microscopy was used to determine whether the donor molecules truly permeate through the cell membrane or only adsorb to the cell surface. This method was found to be partially successful since the donor red tail fluorescence overlapped with the sensitized acceptor fluorescence and led to false reading of FRET. We found that is easier to monitor delivery of acceptor molecules into donor-labeled cells. Using donor labeled cells it was possible to determine whether the acceptor molecules were actually delivered into cells. However, a relatively high acceptor concentration in the hundreds of micromolar level was needed to obtain measurable FRET signals in the 3-D cellular system. The results underscored the need to reduce the dimensionality of FRET systems in order to increase the FRET efficiency between donor and acceptor molecules. Chapter 4 describes the development of FRET sensing lipobeads labeled with donors and their use to evaluate the interactions of acceptor molecules with the phospholipid membrane of FRET sensing lipobeads. The change in the dimensionality of the system in which FRET occurs, improved the sensitivity of our measurements by 3-folds compared to FRET measurements in solution. We concluded that a molecular recognition component had to be added to the sensing particles to further increase their selectivity and sensitivity. Chapter 5 describes the development of FRET trap sensing beads and their use for screening nonfluorescent carbohydrates and glycoproteins. The FRET sensing technique was based on binding between dextran molecules labeled with Texas Red (Dextran-TR) and polystyrene microparticles labeled with Fluorescein tagged Concanavalin A (FITC-ConA). It was found that carbohydrates and glycoproteins inhibit the binding between dextran-TR and FITC-ConA labeled particles. The inhibition effect was concentration dependent thus enabled screening carbohydrates and glycoproteins based on their inhibition potency. The dissertation critically evaluates the performance of FRET microscopy and FRET based sensors in delivery and screening applications.
Download or read book Make Life Visible written by Yoshiaki Toyama and published by Springer Nature. This book was released on 2019-10-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Download or read book Photochemistry and Photophysics of Polymeric Materials written by Norman S. Allen and published by John Wiley & Sons. This book was released on 2010-03-18 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents the state of the technology, from fundamentals to new materials and applications Today's electronic devices, computers, solar cells, printing, imaging, copying, and recording technology, to name a few, all owe a debt to our growing understanding of the photophysics and photochemistry of polymeric materials. This book draws together, analyzes, and presents our current understanding of polymer photochemistry and photophysics. In addition to exploring materials, mechanisms, processes, and properties, the handbook also highlights the latest applications in the field and points to new developments on the horizon. Photochemistry and Photophysics of Polymer Materials is divided into seventeen chapters, including: Optical and luminescent properties and applications of metal complex-based polymers Photoinitiators for free radical polymerization reactions Photovoltaic polymer materials Photoimaging and lithographic processes in polymers Photostabilization of polymer materials Photodegradation processes in polymeric materials Each chapter, written by one or more leading experts and pioneers in the field, incorporates all the latest findings and developments as well as the authors' own personal insights and perspectives. References guide readers to the literature for further investigation of individual topics. Together, the contributions represent a series of major developments in the polymer world in which light and its energy have been put to valuable use. Not only does this reference capture our current state of knowledge, but it also provides the foundation for new research and the development of new materials and new applications.
Download or read book Fluorescent Analogs of Biomolecular Building Blocks written by Marcus Wilhelmsson and published by John Wiley & Sons. This book was released on 2016-04-04 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.
Download or read book Rapid Detection and Identification of Infectious Agents written by David Kingsbury and published by Elsevier. This book was released on 2012-12-02 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Detection and Identification of Infectious Agents is a collection of papers presented at the International Symposium on Rapid Detection and Identification of Infectious Agents held on October 5-7, 1983, in Oakland, California, and organized by the Naval Biosciences Laboratory of the School of Public Health of the University of California at Berkeley. Contributors examine progress in the field of rapid diagnosis of infectious diseases, with a particular emphasis on DNA probe-based assays and monoclonal and polyclonal antibody-based immunoassays. This volume is organized into five sections encompassing 20 chapters. It begins with an overview of state-of-the-art methods for rapid detection and identification of infectious agents, including technology that is currently applied in clinical microbiology, as well as concerns regarding the political and scientific climates, which have an impact on health care and clinical microbiology. Chapters are organized to deal with a single diagnostic type of test for a given broad group of organisms. The approach is to compare the strengths and weaknesses of each of the new diagnostic procedures, using the same type of clinical material whenever possible. The book gives consideration to the fundamental design of DNA probes and probe assay systems, the clinical comparison of immunologic assays for the diagnosis of meningococcal disease, and immunodiagnostics for viral and parasitic pathogens. This book will be of value to scientists and researchers interested in immunology and infectious diseases, as well as the methods used to detect and identify them.
Download or read book FRET and FLIM Techniques written by Theodorus W. J. Gadella and published by Elsevier. This book was released on 2011-07-29 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Download or read book Fluorescence Resonance Energy Transfer FRET Systems for Biomedical Sensor Applications written by Aoibhéann Bird and published by . This book was released on 2010 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Immunoassay Technologies written by Sandeep K. Vashist and published by Academic Press. This book was released on 2018-01-02 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. - Provides a valuable increase of understanding of cellular and biomedical functions - Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings - Discusses all multifarious aspects of immunoassays - Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications - Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays
