Download or read book Analysis of Bacteroidales 16S RRNA Gene Sequences from a Geographically Isolated Human Population written by Carolyn A. Petersen and published by . This book was released on 2006 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Standard methods of measuring fecal pollution in water do not distinguish between human and non-human sources. Molecular technology enabled the development of host-specific markers that distinguish fecal sources. Human specific PCR primers, HF183F and HF134F, were designed based on phylogenetic analyses of partial 16S rRNA gene sequences from the Bacteroidales group of fecal anaerobes. Both primers amplify human fecal DNAs in the U.S., Europe, New Zealand, and Japan. However, they did not amplify human fecal DNAs from a geographically isolated population in Alaska, although amplification was possible with general Bacteroidales primers. We undertook phylogenetic analysis to compare Bacteroidales 16S rRNA genes from the isolated population to a non-isolated population. Our ultimate objective was to create new Bacteroidales human-specific primers from the full-length 16S rRNA gene to amplify fecal DNAs from both isolated and non-isolated geographic areas. Sequence libraries from the isolated Alaskan population and from Oregon were created by amplifying the full-length Bacteroidales 16S rRNA gene. Fragments were cloned and sequenced and 96 colonies from each geographic location were screened for inserts. Phylogenetic analysis and primer design used ARB software. Humans in the Alaskan isolated population did not have sequences from certain common human Bacteroidales groups, and also contained unique clades. The trees constructed showed that none of the Alaskan sequences grouped with sequences from which the current human primers were developed, explaining why fecal samples from this population did not amplify with those primers. A novel clade only contained human sequences from the Alaskan study and was one focus for primer design. Primers were designed from the sequences found in these two clades and from other human-specific clades. A promising primer in the latter half of the 16S rRNA gene targeted human-specific fecal bacteria in both Oregon and Alaskan populations. Humans in the isolated Alaskan population may differ in their fecal bacteria for reasons of geographic isolation, population history, or diet.
Download or read book Novel Approaches to Analysing 16S Ribosomal RNA Gene Sequencing Profiles of the Gut Microbiota in Human Health Research written by Matthew Jackson and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: From this, I generate an index that can represent widescale gut microbiota composition within a single score that is also associated with host health. Secondly, I present a method to identify communities of interacting microbes within the gut microbiota and demonstrate that these reliably associate with host phenotypes across geographically diverse populations. In summary, this thesis addresses several issues currently associated with the analysis of 16S rRNA gene sequencing-based gut microbiota profiles and in turn identifies several novel biological phenomena. The techniques described herein should improve the utility of microbiota profiles derived from 16S rRNA gene sequencing and advance the field of gut microbiome research in human health.
Download or read book Abstracts of the General Meeting of the American Society for Microbiology written by American Society for Microbiology. General Meeting and published by . This book was released on 2008 with total page 828 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Source Tracking written by Jorge W. Santo Domingo and published by Emerging Issues in Food Safety. This book was released on 2007 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.
Download or read book The Fecal Bacteria written by Michael J. Sadowsky and published by American Society for Microbiology Press. This book was released on 2010-11-19 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: A unique, holistic approach to understanding fecal bacteria. • Offers a balanced, integrated discussion of fecal bacteria and their presence and ecology in the intestinal tract of mammals, in the environment, and in the food supply. • Covers the use of fecal bacteria to examine and assess water quality to offer protection from illnesses related to swimming in or ingesting contaminated water, in addition to discussing their use in engineering considerations of water quality, modeling, monitoring, and regulations. • Includes perspectives from an internationally recognized group of experts that integrates medicine, public health, environmental, and microbiological topics. • Serves as a resource for microbiologists, clinicians, animal scientists, engineers, environmental scientists, food safety experts, water quality managers, and students.
Download or read book Microbiome and Metabolome in Diagnosis Therapy and other Strategic Applications written by Joel Faintuch and published by Academic Press. This book was released on 2019-01-03 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiome and Metabolome in Diagnosis, Therapy, and Other Strategic Applications is the first book to simultaneously cover the microbiome and the metabolome in relevant clinical conditions. In a pioneering fashion, it addresses not only the classic intestinal environment, but also the oral, gastric, lung, skin and vaginal microbiome that is in line with the latest investigations. Nonbacterial microbiomes, such as fungi and viruses are not overlooked, and the plasma microbiome is also discussed. As plasma, brain, placenta, tumor cells, and other sterile fluids and tissues, are increasingly recognized to potentially host a microbiome, albeit a limited one, this is a timely resource. The book's editors were fortunate to have the input of renowned collaborators from nearly all continents. This is truly an international effort that brings the latest in the field to students and professionals alike. - Provides comprehensive coverage on diagnosis, therapy, pharmacotherapy and disease prevention in context of the microbiome and metabolome - Focuses on the proposed physiological or pathological conditions - Presents an up-to-date, useful reference
Download or read book The Social Biology of Microbial Communities written by Institute of Medicine and published by National Academies Press. This book was released on 2013-01-10 with total page 633 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the germ theory of disease in the 19th century and extending through most of the 20th century, microbes were believed to live their lives as solitary, unicellular, disease-causing organisms . This perception stemmed from the focus of most investigators on organisms that could be grown in the laboratory as cellular monocultures, often dispersed in liquid, and under ambient conditions of temperature, lighting, and humidity. Most such inquiries were designed to identify microbial pathogens by satisfying Koch's postulates.3 This pathogen-centric approach to the study of microorganisms produced a metaphorical "war" against these microbial invaders waged with antibiotic therapies, while simultaneously obscuring the dynamic relationships that exist among and between host organisms and their associated microorganisms-only a tiny fraction of which act as pathogens. Despite their obvious importance, very little is actually known about the processes and factors that influence the assembly, function, and stability of microbial communities. Gaining this knowledge will require a seismic shift away from the study of individual microbes in isolation to inquiries into the nature of diverse and often complex microbial communities, the forces that shape them, and their relationships with other communities and organisms, including their multicellular hosts. On March 6 and 7, 2012, the Institute of Medicine's (IOM's) Forum on Microbial Threats hosted a public workshop to explore the emerging science of the "social biology" of microbial communities. Workshop presentations and discussions embraced a wide spectrum of topics, experimental systems, and theoretical perspectives representative of the current, multifaceted exploration of the microbial frontier. Participants discussed ecological, evolutionary, and genetic factors contributing to the assembly, function, and stability of microbial communities; how microbial communities adapt and respond to environmental stimuli; theoretical and experimental approaches to advance this nascent field; and potential applications of knowledge gained from the study of microbial communities for the improvement of human, animal, plant, and ecosystem health and toward a deeper understanding of microbial diversity and evolution. The Social Biology of Microbial Communities: Workshop Summary further explains the happenings of the workshop.
Download or read book The Prokaryotes written by Edward F. DeLong and published by Springer. This book was released on 2013-03-22 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea
Download or read book The Gut Brain Axis written by Niall Hyland and published by Academic Press. This book was released on 2016-05-13 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Gut-Brain Axis: Dietary, Probiotic, and Prebiotic Interventions on the Microbiota examines the potential for microbial manipulation as a therapeutic avenue in central nervous system disorders in which an altered microbiota has been implicated, and explores the mechanisms, sometimes common, by which the microbiota may contribute to such disorders. - Focuses on specific areas in which the microbiota has been implicated in gut-brain communication - Examines common mechanisms and pathways by which the microbiota may influence brain and behavior - Identifies novel therapeutic strategies targeted toward the microbiota in the management of brain activity and behavior
Download or read book Metagenomics of the Human Body written by Karen E. Nelson and published by Springer Science & Business Media. This book was released on 2010-11-16 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book brings a completely different perspective than available books by combining the information gained from the human genome with that derived from parallel metagenomic studies, and new results from investigating the effects of these microbes on the host immune system. Although there are a number of books that focus on the human genome that are currently available, there are no books that bring to the forefront the mix of the human genome and the genomes and metagenomes of the microbial species that live within and on us.
Download or read book Metabolic Interaction in Infection written by Ricardo Silvestre and published by Springer. This book was released on 2018-04-06 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on host–pathogen interactions at the metabolic level. It explores the metabolic requirements of the infectious agents, the microbial metabolic pathways that are dedicated to circumvent host immune mechanisms as well as the molecular mechanisms by which pathogens hijack host cell metabolism for their own benefit. Finally, it provides insights on the possible clinical and immunotherapeutic applications, as well as on the available experimental and analytical methods. The contributions break new ground in understanding the metabolic crosstalk between host and pathogen.
Download or read book The Prokaryotes written by Stanley Falkow and published by Springer Science & Business Media. This book was released on 2006-11-14 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: The revised Third Edition of The Prokaryotes, acclaimed as a classic reference in the field, offers new and updated articles by experts from around the world on taxa of relevance to medicine, ecology and industry. Entries combine phylogenetic and systematic data with insights into genetics, physiology and application. Existing entries have been revised to incorporate rapid progress and technological innovation. The new edition improves on the lucid presentation, logical layout and abundance of illustrations that readers rely on, adding color illustration throughout. Expanded to seven volumes in its print form, the new edition adds a new, searchable online version.
Download or read book Evolving Human Nutrition written by Stanley J. Ulijaszek and published by Cambridge University Press. This book was released on 2012-10-18 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exploration of changing human nutrition from evolutionary and social perspectives and its influence on health and disease, past and present.
Download or read book Endodontic Microbiology written by Ashraf F. Fouad and published by John Wiley & Sons. This book was released on 2017-02-06 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endodontic Microbiology, Second Edition presents a comprehensive reference to the microbiology, pathogenesis, management, and healing of endodontic pathosis, emphasizing the importance of biological sciences in understanding and managing endodontic disease and its interaction with systemic health. Provides a major revision to the first book to focus on the problems related to microbes in the root canal and periapical tissues Updates current knowledge in endodontic pathosis, especially regarding next generation sequencing and microbial virulence Presents useful diagrams, images, radiographs, and annotated histological images to illustrate the concepts Emphasizes the importance of biological science in understanding and managing endodontic disease Includes contributions from the leading researchers and educators in the field
Download or read book Water and Health written by Prati Pal Singh and published by Springer Science & Business Media. This book was released on 2013-10-01 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Water and Health” strengthens the dynamic relationship between human health and water. The book has the potential to ignite our minds about several water-related diseases due to biological and chemical contamination, and to their high-end solutions. The contents are original, comprehensive and highly informative, and gradually take the reader around the component most important to his or her quality life, and not just existence. The book is set in social, scientific and economic dimensions, and is a must read for all those who cherish and celebrate human life and dignity.
Download or read book Inflammation and Immunity in Depression written by Bernhard Baune and published by Academic Press. This book was released on 2018-05-23 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt: Inflammation and Immunity in Depression: Basic Science and Clinical Applications is the first book to move beyond the established theory of cytokine-induced depression and explore the broader role the immune system plays in this devastating mood disorder. The book fully explores the most recent lines of research into this rapidly advancing field, including alterations of T-cells, the neurobiological implications of neuroinflammation and immune alterations for brain development and function, and the genetic components of neuroinflammation in depression, including the relationships between stress and inflammation that are revealing gene-environment interactions in the disorder. Combining contributions from researchers worldwide, this book provides the most comprehensive discussion available today on the involvement of the innate immune and adaptive immune systems in depressive disorder. Chapters span neuroscience, psychology, clinical applications and future directions, making this book an invaluable resource for advanced students, researchers and practitioners who need to understand the complex and varied role of inflammation and immune responses in depression. - Synthesizes current knowledge of inflammation and immunity in depression, ranging from basic neuroscience research, to clinical applications in psychiatry - Expands on the long-established theory of cytokine-induced depression to discuss broader involvement of the immune system - Explores translational potential of targeting immune dysfunction for clinical interventions
Download or read book Seafoods Chemistry Processing Technology and Quality written by Fereidoon Shahidi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Seafoods are important sources of nutrients for humans. Proteins and non protein nitrogenous compounds play an important role in the nutritional value and sensory quality of seafoods. Consumption of fish and marine oils is also actively encouraged for the prevention and treatment of cardio vascular diseases and rheumatoid arthritis. Highly unsaturated long-chain omega-3 fatty acids are regarded as the active components of marine oils and seafood lipids. The basic chemical and biochemical properties of seafood proteins and lipids, in addition to flavour-active components, their microbiological safety and freshness quality, are important factors to be considered. A presentation of the state-of-the-art research results on seafoods with respect to their chemistry, processing technology and quality in one volume was made possible by cooperative efforts ofan international group of experts. Following a brief overview, the book is divided into three sections. In Part 1 (chapters 2 to 8) the chemistry of seafood components such as proteins, lipids, flavorants (together with their properties and nutritional significance) is discussed. Part 2 (chapters 9 to 13) describes the quality of seafoods with respect to their freshness, preservation, micro biological safety and sensory attributes. The final section of the book (chapters 14 to 16) summarizes further processing of raw material, underutilized species and processing discards for production of value added products.
