Download or read book Agrobacterium Tumefaciens written by Eugene W. Nester and published by American Phytopathological Society. This book was released on 2005 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This anthology traces the fascinating progress from plant pathology to biotechnology through 38 scientific papers on Agrobacterium, published over the past century. Included are the seminal scientific papers on the biology and application of Agrobacterium with introductory commentaries mostly by those involved in the original work. The commentaries give background to the papers and explain the problems faced and the techniques used, providing insight into the way fundamental research progresses.Agrobacterium tumefaciens has played a major role in the astounding advances that have been made over the past several decades in the areas of plant genetics, plant molecular biology, and plant genetic engineering. The papers included in this book were integral to the current understanding of the interaction of Agrobacterium with its hosts, its development into a major player in the genetic engineering of plants, and the biological control of crown gall.Agrobacterium tumefaciens: From Plant Pathology to Biotechnology is divided into five sections. The first section begins with 1904 when Erwin F. Smith began detailed work on crown gall and considered it to be a plant pathological problem. It explores many of the biological discoveries made over the past century, including the pivotal moment when Armin C. Braun discovered that crown gall was a plant cancer. Other papers cover the beginnings of T-DNA research and the development of vectors to improve the process of transferring T-DNA from bacterium to plant cell. The second section delves further into vector systems and genetic coding for disease and insect resistance, exploring the evolution of genetic engineering in crops.The final three sections deal with themes developed from crown gall studies, including ?quorum sensing? or population density, the DNA sequencing of one strain of A. tumefaciens, and the first genetically engineered organism, strain K1026, released for commercial use.According to Editor, Eugene Nester, ?This book should serve as a testimony to the 100 years of research on this remarkable organism, as well as to an international group of investigators who helped reveal secrets of this natural genetic engineer.? Students, professors, plant pathologists, microbiologists, or anyone interested in research and/or the history of plant pathology and biotechnology, will find this collection of papers an intriguing read.From the Preface: ??the journey is not over. As the commentary by Paul Hooykaas indicates, it looks as though T-DNA will insert into any cell, be it plant, fungal or even mammalian. Is there a possibility of using Agro?bacterium in gene therapy? Will Agrobacterium prove to be as useful a tool in fungal genetics as it has been in plant genetics? Its potential is mind-boggling.?
Download or read book Agrobacterium From Biology to Biotechnology written by Tzvi Tzfira and published by Springer Science & Business Media. This book was released on 2007-12-25 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
Download or read book Plant Transformation via Agrobacterium Tumefaciens written by Phetole Mangena and published by CRC Press. This book was released on 2022-09-01 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.
Download or read book Agrobacterium Protocols written by Kan Wang and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Download or read book Plant Genetic Engineering written by A.D. Arencibia and published by Elsevier. This book was released on 2000-02-14 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biotechnology offers important opportunities for agriculture, horticulture, and the pharmaceutical and food industry by generating transgenic varieties with altered properties. This is likely to change farming practice and reduce the potential negative impact of plant production on the environment. This volume shows the worldwide advances and potential benefits of plant genetic engineering focusing on the third millennium. The authors discuss the production of transgenic plants resistant to biotic and abiotic stress, the improvement of plant qualities, the use of transgenic plants as bioreactors, and the use of plant genomics for genetic improvement and gene cloning. Unique to this book is the integrative point of view taken between plant genetic engineering and socioeconomic and environmental issues. Considerations of regulatory processes to release genetically modified plants, as well as the public acceptance of the transgenic plants are also discussed.This book will be welcomed by biotechnologists, researchers and students alike working in the biological sciences. It should also prove useful to everyone dedicated to the study of the socioeconomic and environmental impact of the new technologies, while providing recent scientific information on the progress and perspectives of the production of genetically modified plants. The work is dedicated to Professor Marc van Montagu.
Download or read book Plant Tissue Culture Manual written by K. Lindsey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.
Download or read book Discoveries In Plant Biology Volume I written by Shain-dow Kung and published by World Scientific. This book was released on 1998-02-17 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: As scientific progress hinges on the continual discovery and extension of previous discoveries, this series, Discoveries in Plant Biology, is specially compiled to provide an atlas of the landmark discoveries in the broad span of plant biology. The collection of chapters, written by renowned plant biologists, describe how classic discoveries were made and how they have served as the foundation for subsequent discoveries. We hope that this will facilitate our readers' quest to advance their knowledge based on the advancements made previously by others.The 21 discoveries described in this First Volume all form the foundations of modern plant biology. The contributors, many of whom are themselves the researchers who made the discoveries, bring readers back in time to retrace the steps of the discoveries. Following the creative thoughts of the scientists in deciphering the natural laws, readers may appreciate how each field was developed from a simple subject to an advanced multidisciplinary field.
Download or read book Agrobacterium biology and its application to transgenic plant production written by Hau-Hsuan Hwang and published by Frontiers Media SA. This book was released on 2015-06-26 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.
Download or read book Genetic Engineering for Nitrogen Fixation written by Alexander Hollaender and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a time in scientific research when a number of developments coincide making it possible to progress with a tough and complicated problem. It is believed that such a time has come in the area of biological nitrogen fixation. A better understanding of photosynthesis, cell hybridization, plasmid, and gene transfer between cells not necessarily genetically related, have opened new avenues of research. New developments in traditional genetics, cell biology, biochemistry, including enzyme chemistry, and plant physi ology have brought about the feeling this is a most appro priate time to pull together the different approaches in a conference where the lines of research could be discussed and thus help to speed up developments in this area. What makes biological nitrogen fixation especially im portant is the promise that a good understanding of the basic problem would help us to make organisms more amenable to fix nitrogen, not only in symbiosis with legumes, but also with other plant species and develop a wider variety of organisms with the ability to fix N • It will also 2 encourage a search for naturally occurring N2 fixing organ isms other than the traditional N2 fixers. Some success has already been encountered in this area. Success in broadening the field of nitrogen fixing would help to increase food supply, especially in de veloping countries which cannot afford to purchase synthetic nitrogen sources.
Download or read book Gene Transfer to Plants written by Ingo Potrykus and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Agrobacterium Protocols written by Kan Wang and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Download or read book Molecular Mechanisms of Bacterial Virulence written by C.I. Kado and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 676 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growing body of information on bacteria pathogenic for humans, mammals and plants generated within the past ten years has shown the interesting conservation of newly identified genes that playa direct role in the pathogenic mechanism. In addition to these genes, there are also genes that confer host specificities and other traits important in pathogenesis on these pathogens. In this volume, we have organized the subject areas to best fit the concept on the way bacterial pathogens recognize, interact and invade the host, on the regulation of genes involved in virulence, on the genes involved in the elaboration of toxins and other pathogenic components such as iron sequestering proteins, and on the mechanisms of circumventing the host defense systems. These areas are divided into Sections. Section I covers the first step when the pathogen seeks its host, and Sections II through VI cover subsequent steps leading to pathogenesis while avoiding host defenses. We conclude this work with a chapter summarizing information on examples of virulence mechanisms that are highly conserved.
Download or read book Molecular Biology of Plant Tumors written by Günter Kahl and published by . This book was released on 1982 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Agrobacterium Biology written by Stanton B. Gelvin and published by Springer. This book was released on 2018-11-28 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to pathogenesis, bacterial cell biology, plant and fungal transformation, natural transgenics, and biotechnology. Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern biotechnology applications derive from more than 40 years of intensive basic scientific research. Many of the biological principles established by this research have served as models for other bacteria, including human and animal pathogens. Written by leading experts and highlighting recent advances, this volume serves both as an introduction to Agrobacterium biology for students as well as a more comprehensive text for research scientists.
Download or read book Advances in Wheat Genetics From Genome to Field written by Yasunari Ogihara and published by Springer. This book was released on 2015-09-15 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: This proceedings is a collection of 46 selected papers that were presented at the 12th International Wheat Genetics Symposium (IWGS). Since the launch of the wheat genome sequencing project in 2005, the arrival of draft genome sequences has marked a new era in wheat genetics and genomics, catalyzing rapid advancement in the field. This book provides a comprehensive review of the forefront of wheat research, across various important topics such as germplasm and genetic diversity, cytogenetics and allopolyploid evolution, genome sequencing, structural and functional genomics, gene function and molecular biology, biotic stress, abiotic stress, grain quality, and classical and molecular breeding. Following an introduction, 9 parts of the book are dedicated to each of these topics. A final, 11th part entitled “Toward Sustainable Wheat Production” contains 7 excellent papers that were presented in the 12th IWGS Special Session supported by the OECD. With rapid population growth and radical climate changes, the world faces a global food crisis and is in need of another Green Revolution to boost yields of wheat and other widely grown staple crops. Although this book focuses on wheat, many of the newly developed techniques and results presented here can be applied to other plant species with large and complex genomes. As such, this volume is highly recommended for all students and researchers in wheat sciences and related plant sciences and for those who are interested in stable food production and food security.
Download or read book The Rhizobiaceae written by Herman P. Spaink and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Rhizobiaceae, Molecular Biology of Model Plant-Associated Bacteria. This book gives a comprehensive overview on our present molecular biological knowledge about the Rhizobiaceae, which currently can be called the best-studied family of soil bacteria. For many centuries they have attracted the attention of scientists because of their capacity to associate with plants and as a consequence also to specifically modify plant development. Some of these associations are beneficial for the plant, as is the case for the Rhizobiaceae subgroups collectively called rhizobia, which are able to fix nitrogen in a symbiosis with the plant hosts. This symbiosis results in the fonnation of root or stem nodules, as illustrated on the front cover. In contrast, several Rhizobiaceae subgroups can negatively affect plant development and evoke plant diseases. Examples are Agrobacterium tumefaciens andA. rhizogenes which induce the formation of crown galls or hairy roots on the stems of their host plants, respectively (bottom panels on front cover). In addition to the obvious importance of studies on the Rhizobiaceae for agronomy, this research field has resulted in the discovery of many fundamental scientific principles of general interest, which are highlighted in this book. To mention three examples: (i) the discovery of DNA transfer of A.
Download or read book Agrobacterium Protocols written by Kan Wang and published by Springer. This book was released on 2014-11-22 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.
