Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Download or read book Applied Biophysics for Drug Discovery written by Donald Huddler and published by John Wiley & Sons. This book was released on 2017-10-02 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Techniques in Molecular Biology written by John M. Walker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin ology, a glossary of terms has been included.
Download or read book Process Scale Purification of Antibodies written by Uwe Gottschalk and published by John Wiley & Sons. This book was released on 2017-03-07 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing
Download or read book Affinity Chromatography written by Pascal Bailon and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Download or read book Design and Synthesis of Organic Molecules Based on Molecular Recognition written by Georges van Binst and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: A topic for a Solvay Conference should be general enough to conform with the great tradition of previous conferences. On the other hand it should not be so broad that it can not be covered during the limited time of the conference. After discussion of several such topics, "Design and Synthesis of Organic Molecules Based on Molecular Recognition" was chosen. According to Albert Einstein we live in an era of perfect methods and confused aims. For example in organic chemistry the known synthetic methods allow us to prepare an astronomical number of compounds; the gap between the possible and the relevant becomes larger every day. After discovery and classification of the main types of reactions, the study of the selectivity of reactions becomes of paramount importance. One can learn quite a lot from Nature, which uses molecular recognition to achieve selec tivity in a degree so far unattainable by mere mortals. To analyze the structural features applied by Nature, to accomplish high molecular recog nition, and to simulate these features by synthesis have recently become therefore favorite occupations of chemists. The purpose of this conference was to bring together two groups, the analysts and the syntheticists, to discuss the present status of the knowledge. This monograph contains the chairman's introduction in which he has summarized the main points at issue and the contributions of the renowned scientists who participated. The organizers hope that it will stimulate further research in this fascinating field.
Download or read book Monolithic Materials written by F. Svec and published by Elsevier. This book was released on 2003-04-29 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, monolithic materials in the shape of discs, stacked layers, rolled sheets, sponges, irregular chunks, tubes, and cylinders have all been successfully demonstrated. These formats were prepared from a wide variety of materials including natural polymers such as cellulose, synthetic polymers that involved porous styrene-, methacrylate-, and acrylamide-based polymers, and inorganic materials, mainly silica. Each approach is interesting from the point of view of both preparation and application.Although the current papers and patents concerned with monolithic separation media are quite numerous, the information is scattered throughout a vast number of journals. This book therefore fills the gap in the market for a comprehensive reference book on this subject.Monolithic materials concerns all of the current formats of monolithic materials and provides an integrated view of this novel format of separation media. Since the flow pattern in monolithic devices is different from that in packed beds, the hydrodynamics of the system and mass transport differ considerably from those derived for packed columns. Therefore, this book presents contributions concerned with both flow and mass transfer in the monolithic materials. A significant proportion of the book is devoted to the applications of monolithic materials. It also provides the reader with valuable information about the sources of the specific materials, their properties, and potential applications.·Monolithic materials are currently very popular within several scientific areas such as chromatography, optics, catalysis, diagnostics, genomics, proteomics, and microfluidics.·Provides valuable information about the sources of the specific materials, their properties, and potential applications.·Chapters written by leading experts in the area.
Download or read book Bioaffinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1993-09-14 with total page 819 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography.Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.
Download or read book Biomolecular and Bioanalytical Techniques written by Vasudevan Ramesh and published by John Wiley & Sons. This book was released on 2019-06-10 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
Download or read book Purification and Analysis of Recombinant Proteins written by Ramnath Seetharam and published by CRC Press. This book was released on 1991-01-07 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering both new and traditional topics in the purification and analysis of recombinant proteins, this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work, explaining their theoretical bases. The collection also explores innovative co
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Download or read book Analytical Affinity Chromatography written by Irwin M. Chaiken and published by CRC Press. This book was released on 2018-01-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).
Download or read book Handbook of Thin Layer Chromatography written by Joseph Sherma and published by CRC Press. This book was released on 2003-04-18 with total page 1356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
Download or read book Modern Physical Methods in Biochemistry Part B written by L.L.M. van Deenen and published by Elsevier. This book was released on 1988-07-01 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume completes the account of physical methods used in biochemistry that was covered in Volume 11A, which was published in 1985. It deals with fast reaction methods, high performance liquid chromatography of peptides and proteins, fluorescence spectroscopy, neutron and X-ray scattering techniques, and also Raman and resonance spectroscopy which have in recent years been used to an increasing extent in biochemistry. This addition to the invaluable series New Comprehensive Biochemistry will be of interest to all those working in the fields of biochemistry and biophysics.
