Download or read book Adsorption and Desorption of Proteins from Environmentally and Medically relevant Surfaces written by Hanh Thi My Phan and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein function can be influenced by attachment to surfaces. Understanding of protein adsorption behavior responsible for these functional changes is important for various environmental applications, including prion disease transmission. Infectious prions, a causative agent of prion disease, strongly bind to soil surfaces and medical tools and remain infectious. Studies of prion attachment to soils in the environment and to medical surfaces from the various biologic excreta in which prions are present is limited. To contribute to the knowledge of prion adsorption to surfaces, investigations of different proteins including bovine serum albumin (BSA) and alpha-synuclein adsorption to a variety of environmentally- and medically- relevant surfaces was conducted using quartz crystal microbalance with dissipation and spectroscopic ellipsometry. Adsorption and desorption of BSA, a model protein, varied as a function of surface properties, BSA concentrations, and pH. The initial adsorption rates, adsorbed layer thickness, and molecule arrangements of BSA adsorbed to the various charged surfaces were strongly dependent on the functional tail groups of the surfaces. alpha-Synuclein protein, a surrogate for the prion protein, shares many pathologically common traits with prion protein. Understanding the behavior of alpha-synuclein after binding to environmental surfaces can provide crucial insights into prion conformation and orientation on environmentally- and medically-relevant surfaces. Increased alpha-synuclein adsorption was observed on the environmental surface compared to the medical surface. alpha-Synuclein can bind to multiple surface types and decontamination was surface-dependent. Competitive matrices were also found to influence prion adsorption to soil surfaces. Prions were not likely to adsorb to soil in saliva while increased adsorption of prions were observed in the presence of urine or brain homogenate when compared to a buffer solution. Atomic force microscopy images of prion conformation adsorbed to mica, stainless steel, and SiO 2 surfaces were reported. These data provide evidence of the influences of the competitive matrices on prion adsorption to soil surfaces and fundamental information on prion conformational change upon adsorption to environmentally- and medically-relevant surfaces.
Download or read book Physical Chemistry of Virus Adsorption and Degradation on Inorganic Surfaces written by James Procter Murray and published by . This book was released on 1980 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kinetics of Protein Adsorption desorption Mediated by PH responsive Polymer Layer Project Supported by the National Natural Science Foundation of China Grant Nos 21274062 11474155 and 91027040 written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: We propose a new way of regulating protein adsorption by using a pH-responsive polymer. According to the theoretical results obtained from the molecular theory and kinetic approaches, both thermodynamics and kinetics of protein adsorption are verified to be well controlled by the solution pH. The kinetics and the amount of adsorbed proteins at equilibrium are greatly increased when the solution environment changes from acid to neutral. The reason is that the increased pH promotes the dissociation of the weak polyelectrolyte, resulting in more charged monomers and more stretched chains. Thus the steric repulsion within the polymer layer is weakened, which effectively lowers the barrier felt by the protein during the process of adsorption. Interestingly, we also find that the kinetics of protein desorption is almost unchanged with the variation of pH. It is because although the barrier formed by the polymer layer changes along with the change of pH, the potential at contact with the surface varies equally. Our results may provide useful insights into controllable protein adsorption/desorption in practical applications.
Download or read book Environmental Health Perspectives written by and published by . This book was released on 1993 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biological Interactions on Materials Surfaces written by David A. Puleo and published by Springer Science & Business Media. This book was released on 2009-06-26 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success or failure of biomaterials, whether tissue engineered constructs, joint and dental implants, vascular grafts, or heart valves, depends on molecular-level events that determine subsequent responses of cells and tissues. This book presents the latest developments and state-of-the-art knowledge regarding protein, cell, and tissue interactions with both conventional and nanophase materials. Insight into these biomaterial surface interactions will play a critical role in further developments in fields such as tissue engineering, regenerative medicine, and biocompatibility of implanted materials and devices. With chapters written by leaders in their respective fields, this compendium will be the authoritative source of information for scientists, engineers, and medical researchers seeking not only to understand but also to control tissue-biomaterial interactions.
Download or read book Protein Nanoparticle Interactions written by Masoud Rahman and published by Springer Science & Business Media. This book was released on 2013-06-24 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the fabrication of nanomaterials and exploration of their properties have attracted the attention of various scientific disciplines such as biology, physics, chemistry, and engineering. Although nanoparticulate systems are of significant interest in various scientific and technological areas, there is little known about the safety of these nanoscale objects. It has now been established that the surfaces of nanoparticles are immediately covered by biomolecules (e.g. proteins, ions, and enzymes) upon their entrance into a biological medium. This interaction with the biological medium modulates the surface of the nanoparticles, conferring a “biological identity” to their surfaces (referred to as a “corona”), which determines the subsequent cellular/tissue responses. The new interface between the nanoparticles and the biological medium/proteins, called “bio-nano interface,” has been very rarely studied in detail to date, though the interest in this topic is rapidly growing. In this book, the importance of the physiochemical characteristics of nanoparticles for the properties of the protein corona is discussed in detail, followed by comprehensive descriptions of the methods for assessing the protein-nanoparticle interactions. The advantages and limitations of available corona evaluation methods (e.g. spectroscopy methods, mass spectrometry, nuclear magnetic resonance, electron microscopy, X-ray crystallography, and differential centrifugal sedimentation) are examined in detail, followed by a discussion of the possibilities for enhancing the current methods and a call for new techniques. Moreover, the advantages and disadvantages of protein-nanoparticle interaction phenomena are explored and discussed, with a focus on the biological impacts.
Download or read book Prediction of Protein Adsorption on Engineered Surfaces for Biomedical and Industrial Applications written by Maria Eugenia Arias Montecillo and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "This thesis describes the advancement of the Bio-molecular Adsorption Database (BAD) to more accurately predict protein adsorption. To advance the model of the BAD, data was first: collected from published literature in the last ten years, processed to be added to BAD, and used in calculations to advance the BAD ability to accurately predict protein adsorption.Protein adsorption at solid-liquid interfaces is crucial in many biomedical and industrial applications. These applications include biomaterials, protein microarrays, lab-on-a-chip devices and bioreactors. (Vasina et al., 2009)In the biomaterial area, the first response after contact of an implant material with a biological fluid, is the adsorption of proteins on to the surface. The adsorbed protein layer plays a role in the regulation of the biological response to the material and therefore bio-compatibility. It is from the adsorbed protein layer that the cells in the system obtain information about the foreign surface determining the cell behaviour. This cell behaviour can range from: adhesion establishment and proliferation of cells in the proximity to the material or the rejection and inflammation at the interface due to over layering and denaturation. (Wilson, Clegg, Leavesley, & Pearcy, 2005)In the protein microarrays and micro/nano- devices area, protein adsorption is of interest because of the need finding an optimum balance to maximized sensitivity and/or activity for analytical functions. This is done by preserving the proteins native characteristics and minimizing protein denaturation. (Vasina et al., 2009)In the bioreactors area, protein adsorption plays a role in membrane fouling, leading to increased pressures of operation and frequent biofouling clear out. As such, it is important to choose materials and conditions to minimize protein adsorption and biofouling in bioreactors. (Ognier, Wisniewski, & Grasmick, Influence of macromolecule adsorption during filtration of a membrane bioreactor mixed liquor suspension, 2002)Regardless of the considerable attention and research in the last 50 years, protein adsorption prediction had not been attained with sufficient accuracy for engineering applications. The original work into BAD, addressed protein adsorption prediction by archiving protein adsorption data and applying statistical analysis to the data. (Vasina et al., 2009)This approach correlated accurately the input variables to protein adsorption. Examples include: protein concentration in solution, protein characteristics, fluid environment conditions and surface parameters to the output variable or protein concentration on the surface. (Vasina et al., 2009)" --
Download or read book Biomaterials Science written by Buddy D. Ratner and published by Elsevier. This book was released on 2004-08-18 with total page 879 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this bestselling title provides the most up-to-date comprehensive review of all aspects of biomaterials science by providing a balanced, insightful approach to learning biomaterials. This reference integrates a historical perspective of materials engineering principles with biological interactions of biomaterials. Also provided within are regulatory and ethical issues in addition to future directions of the field, and a state-of-the-art update of medical and biotechnological applications. All aspects of biomaterials science are thoroughly addressed, from tissue engineering to cochlear prostheses and drug delivery systems. Over 80 contributors from academia, government and industry detail the principles of cell biology, immunology, and pathology. Focus within pertains to the clinical uses of biomaterials as components in implants, devices, and artificial organs. This reference also touches upon their uses in biotechnology as well as the characterization of the physical, chemical, biochemical and surface properties of these materials. - Provides comprehensive coverage of principles and applications of all classes of biomaterials - Integrates concepts of biomaterials science and biological interactions with clinical science and societal issues including law, regulation, and ethics - Discusses successes and failures of biomaterials applications in clinical medicine and the future directions of the field - Cover the broad spectrum of biomaterial compositions including polymers, metals, ceramics, glasses, carbons, natural materials, and composites - Endorsed by the Society for Biomaterials
Download or read book Adsorption of Microorganisms to Surfaces written by Gabriel Bitton and published by John Wiley & Sons. This book was released on 1980 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface Chemistry in Biomedical and Environmental Science written by Jonathan P. Blitz and published by Springer Science & Business Media. This book was released on 2006-09-09 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume holds a special niche in describing the current state of the art in the fundamentals and applications of a variety of nanomaterials. A common theme throughout much of this volume involves adsorption and interfacial behavior of nanomaterials. The book provides a useful mixture of reviews and primary research from leading laboratories and offers a unique blend of East European and Western contributors.
Download or read book Medical Polymers written by and published by iSmithers Rapra Publishing. This book was released on 2003 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medical Polymers 2003, the third international conference devoted to the use of polymers in the medical industry was held in Dublin, Ireland. The use of polymers in medical technology continues to grow and provides major business opportunities for companies who service the medical industry. Medical Polymers 2003 brought together speakers and delegates from the whole medical device supply chain to identify and address the challenges facing the industry. The conference began with a keynote presentation from the head of European and Regulatory Affairs at the Medical Devices Agency highlighting the many important regulatory issues now facing the industry. Following sessions covered new materials, latest product developments, compatibility of polymers, material advances, analysis and testing of medical polymers.
Download or read book Bioavailability of Contaminants in Soils and Sediments written by National Research Council and published by National Academies Press. This book was released on 2003-05-03 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioavailability refers to the extent to which humans and ecological receptors are exposed to contaminants in soil or sediment. The concept of bioavailability has recently piqued the interest of the hazardous waste industry as an important consideration in deciding how much waste to clean up. The rationale is that if contaminants in soil and sediment are not bioavailable, then more contaminant mass can be left in place without creating additional risk. A new NRC report notes that the potential for the consideration of bioavailability to influence decision-making is greatest where certain chemical, environmental, and regulatory factors align. The current use of bioavailability in risk assessment and hazardous waste cleanup regulations is demystified, and acceptable tools and models for bioavailability assessment are discussed and ranked according to seven criteria. Finally, the intimate link between bioavailability and bioremediation is explored. The report concludes with suggestions for moving bioavailability forward in the regulatory arena for both soil and sediment cleanup.
Download or read book The Kinetics of Protein Adsorption on Synthetic and Modified Natural Surfaces written by Gene Alan Bornzin and published by . This book was released on 1980 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Adsorption on Solid Surfaces written by Sarawut Kaewtathip and published by . This book was released on 2004 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanoparticle Protein Corona written by Ashutosh Kumar and published by Royal Society of Chemistry. This book was released on 2019-07-26 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoparticles have numerous biomedical applications including drug delivery, bone implants and imaging. A protein corona is formed when proteins existing in a biological system cover the nanoparticle surface. The formation of a nanoparticle–protein corona, changes the behaviour of the nanoparticle, resulting in new biological characteristics and influencing the circulation lifetime, accumulation, toxicity, cellular uptake and agglomeration. This book provides a detailed understanding of nanoparticle–protein corona formation, its biological significance and the factors that govern the formation of coronas. It also explains the impact of nanoparticle–protein interactions on biological assays, ecotoxicity studies and proteomics research. It will be of interest to researchers studying the application of nanoparticles as well as toxicologists and pharmaceutical chemists.
Download or read book Proteins at Interfaces II written by and published by . This book was released on 1995 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein adsorption on hydrophobic surfaces from intrinsically unstructured proteins to antibodies written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
