Download or read book A Tale of Three Next Generation Sequencing Platforms written by Applied Research Press and published by Createspace Independent Publishing Platform. This book was released on 2015-07-24 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent's PGM, Pacific Biosciences' RS and the Illumina MiSeq. Here we compare the results obtained with those platforms to the performance of the Illumina HiSeq, the current market leader. In order to compare these platforms, and get sufficient coverage depth to allow meaningful analysis, we have sequenced a set of 4 microbial genomes with mean GC content ranging from 19.3 to 67.7%. Together, these represent a comprehensive range of genome content. Here we report our analysis of that sequence data in terms of coverage distribution, bias, GC distribution, variant detection and accuracy. All three fast turnaround sequencers evaluated here were able to generate usable sequence. However there are key differences between the quality of that data and the applications it will support. Proceeds from the sale of this book go to the support of an elderly disabled person.
Download or read book Next Generation Sequencing written by Jerzy Kulski and published by BoD – Books on Demand. This book was released on 2016-01-14 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.
Download or read book Computational Methods for Next Generation Sequencing Data Analysis written by Ion Mandoiu and published by John Wiley & Sons. This book was released on 2016-10-03 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces readers to core algorithmic techniques for next-generation sequencing (NGS) data analysis and discusses a wide range of computational techniques and applications This book provides an in-depth survey of some of the recent developments in NGS and discusses mathematical and computational challenges in various application areas of NGS technologies. The 18 chapters featured in this book have been authored by bioinformatics experts and represent the latest work in leading labs actively contributing to the fast-growing field of NGS. The book is divided into four parts: Part I focuses on computing and experimental infrastructure for NGS analysis, including chapters on cloud computing, modular pipelines for metabolic pathway reconstruction, pooling strategies for massive viral sequencing, and high-fidelity sequencing protocols. Part II concentrates on analysis of DNA sequencing data, covering the classic scaffolding problem, detection of genomic variants, including insertions and deletions, and analysis of DNA methylation sequencing data. Part III is devoted to analysis of RNA-seq data. This part discusses algorithms and compares software tools for transcriptome assembly along with methods for detection of alternative splicing and tools for transcriptome quantification and differential expression analysis. Part IV explores computational tools for NGS applications in microbiomics, including a discussion on error correction of NGS reads from viral populations, methods for viral quasispecies reconstruction, and a survey of state-of-the-art methods and future trends in microbiome analysis. Computational Methods for Next Generation Sequencing Data Analysis: Reviews computational techniques such as new combinatorial optimization methods, data structures, high performance computing, machine learning, and inference algorithms Discusses the mathematical and computational challenges in NGS technologies Covers NGS error correction, de novo genome transcriptome assembly, variant detection from NGS reads, and more This text is a reference for biomedical professionals interested in expanding their knowledge of computational techniques for NGS data analysis. The book is also useful for graduate and post-graduate students in bioinformatics.
Download or read book Algorithms for Next Generation Sequencing Data written by Mourad Elloumi and published by Springer. This book was released on 2017-09-18 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 14 contributed chapters in this book survey the most recent developments in high-performance algorithms for NGS data, offering fundamental insights and technical information specifically on indexing, compression and storage; error correction; alignment; and assembly. The book will be of value to researchers, practitioners and students engaged with bioinformatics, computer science, mathematics, statistics and life sciences.
Download or read book Microbial Technology for the Welfare of Society written by Pankaj Kumar Arora and published by Springer Nature. This book was released on 2019-09-12 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes various aspects of modern microbiology including microbial enzymes, secondary metabolites, next-generation sequencing, microbial-based biopesticides, microbial-based cancer therapies, biodiesel, and microbial products from fermentation, biodegradation, bioremediation and wastewater treatment. Further, it explains how and why microbes play an important role in preserving the welfare of living beings and the environment. Many bacteria play a significant part in cleaning our environment by detoxifying various xenobiotic compounds, while several microbes produce secondary metabolites that are useful to human beings. The book is divided into 15 chapters that cover various aspects of microorganism-based biotechnology, including recent methodologies such as advanced molecular techniques, as well developments in classical microbiological techniques. The authors also explain how the latest and classical techniques are being used in modern-day microbial biotechnology. All chapters were written by experts from prominent universities, research laboratories, and institutes around the globe. Above all, they focus on recent advances in microbial technology that promote the welfare of living beings and the environment.
Download or read book Advances in Bioinformatics written by Vijai Singh and published by Springer Nature. This book was released on with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Omics Trends and Applications written by Khalid Rehman Hakeem and published by Springer. This book was released on 2016-08-23 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: To comprehend the organizational principle of cellular functions at diff erent levels, an integrative approach with large-scale experiments, the so-called ‘omics’ data including genomics, transcriptomics, proteomics, and metabolomics, is needed. Omics aims at the collective characterization and quantifi cation of pools of biological molecules that translate into the structure, function, and dynamics of an organism or organisms. Currently, omics is an essential tool to understand the molecular systems that underlie various plant functions. Furthermore, in several plant species, the development of omicsresources has progressed to address the particular biological properties of individual species. Integration of knowledge from omics-based research is an emerging issue as researchers seek to identify significance, gain biological insights and promote translational research. From these perspectives, we intend to provide the emerging aspects of plant systems research based on omics and bioinformatics analyses together with their associated resources and technological advances. Th e present book covers a wide range of omics topics, and discusses the latest trends and application area of plant sciences. In this volume, we have highlighted the working solutions as well as open problems and future challenges in plant omics studies. We believe that this book will initiate and introduce readers to state-of-the-art developments and trends in omics-driven research.
Download or read book Next Generation Sequencing and Data Analysis written by Melanie Kappelmann-Fenzl and published by Springer Nature. This book was released on 2021-05-04 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook provides step-by-step protocols and detailed explanations for RNA Sequencing, ChIP-Sequencing and Epigenetic Sequencing applications. The reader learns how to perform Next Generation Sequencing data analysis, how to interpret and visualize the data, and acquires knowledge on the statistical background of the used software tools. Written for biomedical scientists and medical students, this textbook enables the end user to perform and comprehend various Next Generation Sequencing applications and their analytics without prior understanding in bioinformatics or computer sciences.
Download or read book Next Generation Systematics written by Peter D. Olson and published by Cambridge University Press. This book was released on 2016-06-16 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: We live in an age of ubiquitous genomics. Next generation sequencing (NGS) technology, both widely adopted and advancing at pace, has transformed the data landscape, opening up an enormous source of heritable characters to the comparative biologist. Its impact on systematics, like many other fields of biology, has been felt throughout its breadth: from defining species boundaries to estimating their evolutionary histories. This volume examines the broad range of ways in which NGS data are being used in systematics and in the fields that it underpins, from biodiversity prospecting to evo-devo. Experts in their fields draw on contemporary case studies to demonstrate state-of-the-art applications of NGS data. These, along with novel analyses, comprehensive reviews and lively perspectives, are combined to produce an authoritative account of contemporary issues in systematics that have been impacted by the adoption of NGS.
Download or read book Next Generation Sequencing Technologies and Challenges in Sequence Assembly written by Sara El-Metwally and published by Springer Science & Business. This book was released on 2014-04-19 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.
Download or read book Next Generation Sequencing NGS Technology in DNA Analysis written by Hirak Ranjan Dash and published by Elsevier. This book was released on 2023-11-30 with total page 614 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows. With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies. - Describes the fundamentals of NGS and its advances for forensic applications - Explains the transition from CE-based technique to NGS technology - Includes case studies related to NGS and DNA fingerprinting - Explores the future use and applications of NGS technologies
Download or read book Unraveling New Frontiers and Advances in Bioinformatics written by Amit Chaudhary and published by Springer Nature. This book was released on with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Entrepreneurship Innovation and Platforms written by Jeffrey Furman and published by Emerald Group Publishing. This book was released on 2017-09-29 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite recent advances in our understanding of how innovation and entrepreneurship impact the creation and appropriation of value, numerous questions remain unanswered. This volume draws together scholars working at the forefront of entrepreneurship-, strategy-, and innovation-related domains to explore these questions.
Download or read book Reproductive Genetics An Issue of Obstetrics and Gynecology Clinics written by Lorraine Dugoff and published by Elsevier Health Sciences. This book was released on 2018-02-11 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This issue provides a timely update for for the ob/gyn on genetics in reproductive medicine. Dr. Dugoff has created an issue with the goals of providing the most currently clinical information on genetic screening and prenatal genetics. Top authors have written reviews on the following topics: Genetic counseling overview for the ob/gyn; Cell-free DNA screening for aneuploidy; Cell-free DNA screening for single gene disorders; The use of microarray in prenatal diagnosis; Whole exome sequencing: Applications in prenatal diagnosis; Screening for aneuploidy in multiple gestations: The challenges and available options; Expanded carrier screening; Ethnicity-based carrier screening overview; Prenatal genetic diagnosis and prenatal genetic screening; Ethical issues in prenatal genetics; Ultrasound findings and associated genetic syndromes; Hereditary cancers in gynecology: and What physicians should know about genetic testing, screening and risk reduction. Readers will come away with the knowledge they need to diagnose, treat, and manage patients based on the most current evidence and data.
Download or read book Molecular Systematics of Parasitic Helminths written by Urusa Thaenkham and published by Springer Nature. This book was released on 2022-05-25 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide fundamental knowledge and information for research in molecular systematics on parasitic helminths (nematode, trematode, cestode). The shreds of evidence of molecular systematics studies will be compiled and discussed in terms of the utilities and pitfalls of the genetic marker used for various purposes, which have been implemented for molecular systematics of parasitic nematodes, cestodes, and trematodes. Moreover, this book will also provide the procedure for research on molecular systematics and DNA taxonomy as the guideline to explore parasitic helminths. Finally, the further perspectives of utilizing genetic markers for molecular studies on parasitic helminths will be addressed in the context of applications from the laboratory to fieldwork such as DNA barcoding and environmental DNA metabarcoding of parasitic helminths. The book will benefit postgraduate students and researchers requiring the detailed knowledge of molecular systematics, as well as researchers desiring a guideline to select genetic markers and analyze DNA sequences to make phylogenetic inferences
Download or read book Synthetic Biology written by Christina Smolke and published by John Wiley & Sons. This book was released on 2018-02-28 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.
Download or read book Molecular Genetics and Genomics Tools in Biodiversity Conservation written by Ashwani Kumar and published by Springer Nature. This book was released on 2022-02-01 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides insight into the use of molecular and genomic techniques to the study of populations of critically important species at various geographical scales. It delves into a wide range of issues relevant to biodiversity conservation, such as population differentiation, landscape genomics, ecological interactions, phylogenetics, phylogeography, metagenomics, molecular methods, and data processing. The current rate of biodiversity loss is unprecedented and valuable genetic resources are being lost at an alarmingly rate. Effective strategies to conserve these genetic resources are essential to maintain healthy ecosystems with inter-dependent species. The book is an invaluable resource for training undergraduate and graduate students, postdoctoral fellows, and for young researchers. This book is particularly useful for the policy makers and academics who want to learn about important concepts in population and conservation genetics and genomics.
