Download or read book X ray Holography of Biological Specimens written by and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The author reviews the reasons for x-ray imaging of biological specimens and the techniques presently being used for x-ray microscopy. The author points out the advantages of x-ray holography and the difficulties of obtaining the requisite coherence with conventional sources. The author discusses the problems of radiation damage and the remarkable fact that short pulse x-ray sources circumvent these problems and obtain high-resolution images of specimens in the living state. Finally, the author reviews some of the efforts underway to develop high-intensity coherent x-ray sources for the laboratory. 14 references, 5 figures, 2 tables.
Download or read book High intensity X ray Holography written by and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The crucial physical and technological issues pertaining to the holographic imaging of biological structures with a short-pulse, high-intensity, high-quantum-energy laser were examined. The limitations of x-ray optics are discussed. Alternative holographic techniques were considered, and it was concluded that far-field Fresnel transform holography (Fraunhofer holography) using a photoresist recording surface is most tractable with near term technology. The hydrodynamic expansion of inhomogeneities within the specimen is discussed. It is shown that expansion is the major source of image blurring. Analytic expressions were derived for the explosion of protein concentrations in an x-ray transparent cytoplasm, compared with numerical calculations, and corrections derived to account for the competitive transport processes by which these inhomogeneities lose energy. It is concluded that for the near term Fresnel transform holography, particularly, far-field or Fraunhofer holography, is more practical than Fourier transform holography. Of the alternative fine grain recording media for use with Fresnel transform holography, a photo-resist is most attractive. For best resolution, exposure times must be limited to a few picoseconds, and this calls for investigation of mechanisms to shutter the laser or gate the recording surface. The best contrast ratio between the nitrogen-bearing polymers (protein and the nucleic acids) and water is between the K-edges of oxygen and nitrogen.
Download or read book X Ray Holographic Microscopy Using Photoresists Recent Developments written by C. Jacobsen and published by . This book was released on 1992 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray holography using photoresists is a method of x-ray microscopy which may be well-suited to flash imaging of radiation-sensitive specimens if suitable sources are developed. We describe our results of imaging dried samples using a synchrotron x-ray source, and our efforts at imaging hydrated biological specimens.
Download or read book X Ray Microscopy in Biology and Medicine written by Kunio Shinohara and published by Springer. This book was released on 1990-04-18 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The papers presented review recent progress in X-ray microscopy both in methodology and in biomedical applications, they compare characteristics of X-ray microscopy with other related forms of microscopy, and they discuss activities of X-ray microscopy in Japan in particular. This review will surely encourage engineers and physicists to accelerate further development of X-ray microscopes to provide even more useful tools to solve biological and medical questions.
Download or read book High intensity X ray Holography written by Johndale C. Solem and published by . This book was released on 1982 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book X ray Microscopy written by Ping-chin Cheng and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1979, a conference on x-ray microscopy was organized by the New York Academy of Sciences, and in 1983, the Second Interna tional Symposium on X-ray Imaging was organized by the Akademie der Wissenschaften in Gottingen, Federal Republic of Germany. This volume contains the contributions to the symposium "X-ray Microscopy '86", held in Taipei, Taiwan, the Republic of China in August 1986. This is the first volume which intends to provide up-to date information on x-ray imaging to biologists, therefore, emphasis was given to specimen preparation techniques and image interpreta tion. Specimen preparation represents a major part of every microscopy work, therefore, it should be strongly emphasized in this emerging field of x-ray microscopy. Theoretically, x-ray microscopy offers the potential for the study of unfixed, hydrated biological ma terials. Since very few biological system can be directly observed without specimen preparation, we would like to emphasize that new information on biological specimens can only be obtained if the speci men is properly prepared. In the past decade, many of the published x-ray images were obtained from poorly prepared biological speci mens, mainly air-dried materials. Therefore, one of the goals of this conference is to bring the importance of specimen preparation to the attention of x-ray microscopy community. X-ray microscopy can be subdivided into several major areas. They are the classic x-ray projection microscope, x-ray contact imag ing (microradiography) and the more recent x-ray scanning micro scope, x-ray photoelectron microscope and x-ray imaging microscope.
Download or read book X ray Holographic Microscopy of Biological Specimens Using an Undulator written by Chris Johnson Jacobsen and published by . This book was released on 1988 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Study on New Approaches in Coherent X ray Microscopy of Biological Specimens written by Klaus Giewekemeyer and published by Universitätsverlag Göttingen. This book was released on 2011 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of coherent x rays for microscopic imaging has seen a rapid and ongoing development within the past decade, driven by an increasing availability of highly brilliant and coherent sources worldwide. Accordingly, novel methods have been developed, which replace the microscope‘s objective lens by a numerical reconstruction scheme. The aim of the present work is to study how very recent experimental and algorithmic developments in the field can be implemented towards a highly sensitive and fully quantitative microscopy method for imaging of biological cells. To this end, different experimental approaches are studied, based on coherent far-field as well as near-field diffraction. At first, an application of the novel ptychographic imaging method to single biological cells is presented. In particular, it is demonstrated how weakly scattering biological specimens can be imaged with fully quantitative density contrast. Alongside, a sueccessful extension of the method towards soft x-ray energies is described.In the second part of the work it is shown how x-ray waveguides can be used as a point source for propagation-based microscopy of single cells in the hard x-ray regime. The specifically devised iterative reconstruction scheme allows for full quantitativity and high sensitivity and thus enables an application to single biological cells. The work contains a thorough introduction into the x-ray optical methods applied and aims at a useful and self-contained overview on aspects of signal and Fourier theory relevant for the used numerical propagation schemes.
Download or read book X Ray Microscopy III written by Alan G. Michette and published by Springer. This book was released on 2013-06-05 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growth of interest and research activity in X -ray microscopy is reflected in the increasing size and scope of a related series of international conferences, the latest of which (XRM90) was held at King's College London (3-7 September 1990) with over 130 delegates. Previous conferences in Gottingen and Brookhaven resulted in books in the Springer Series in Optical Sciences, and this volume, the proceedings of XRM90, maintains this tradition. Because of the large number of papers their lengths were strictly limited and, while most papers can be directly identified with conference presentations, in a few cases those on similar topics by the same authors have been combined into a longer paper to allow better use of the space. The book is divided into six parts, with Parts IT-VI covering the major areas of interest at the conference. In Part 1 are two overviews; Ron Burge presented the opening paper of the conference, while the closing, summary, contrlbution by Janos Kirz is included here as a comprehensive introduction to the remainder of the book. Part IT covers developments in X -ray sources and optics. The high average brightnesses of synchrotron radiation sources have made many applications pos sible, while the more convenient, laboratory-based, plasma sources offer much promise for the future. Several contributions report significant advances in X-ray optics, which must clearly continue fully to exploit the latest sources.
Download or read book X Ray Near Field Holography Beyond Idealized Assumptions of the Probe written by Johannes Hagemann and published by Göttingen University Press. This book was released on 2017 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: All images are flawed, no matter how good your lenses, mirrors etc. are. Especially in the hard X-ray regime it is challenging to manufacture high quality optics due to the weak interaction of multi-keV photons with matter. This is a tremendous challenge for obtaining high resolution quantitative X-ray microscopy images. In recent years lensless phase contrast imaging has become an alternative to classical absorptionbased imaging methods. Without any optics, the image is formed only by the free space propagation of the wave field. The actual image has to be formed posteriori by numerical reconstruction methods. Advanced phasing methods enable the experimentalist to recover a complex valued specimen from a single or a set of intensity measurement. This would be the ideal case - reality teaches us that there are no ideal imaging conditions. Describing, understanding and circumventing these non ideal imaging conditions and their effects on X-ray near-field holographic (NFH) imaging are the leitmotifs for this thesis. In NFH the non ideal conditions manifest themselves in the illuminating wave field or probe. The probe generally does not satisfy the canonical assumptions of fully coherent and monochromatic radiation emitted by a point source. The main results of this thesis are compiled as a collection of publications. An approach is shown to reconstruct the probe of a X-ray nano-focus setup by a series of measurements of the probe at varied Fresnel number. The following chapter presents a study concerning the reconstruction efficiency in terms of resolution for near- and far-field based lensless imaging. In the following, the reconstruction scheme for the probe is extended to incorporate the effects of partial coherence in the near field. This enables the recovery of the modal structure of the probe which yields a full description of its coherence properties. Giving up the assumption of temporal stability due to the stochastic pulses, delivered by X-ray free electron lasers, the reconstruction of probe and specimen must be achieved from a single shot. A suitable scheme for this purpose is proposed in this work.
Download or read book Holography in Medicine and Biology written by G.v. Bally and published by Springer. This book was released on 2013-04-17 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: The International Workshop on Holography in Medicine and Biology was held in MUnster, Federal Republic of Germany, on March 14th and 15th, 1979, at the Clinic of Otorhinolaryngology of the Westfalische Wilhelms-Universitat within the frame of the Symposium 79 of the Sonderforschungsbereich 88 "Teratology and Rehabilitation of Patients with Multiple Handicaps'' of the Deutsche Forschungsgemeinschaft. In fact, this workshop was not the first meeting dealing exclusively with biomedical applications of holography and related techniques. The very first symposium in this field was organized by Prof. P. Greguss and took place in New York in 1973. A second one was held in MUnster in 1976 with the objec tive to improve the communication among the at that time rather isolatedly working groups in this research domain. The great response to that meeting gave encouragement to the organization of another one in MUnster, this time on a more extended international base. Thus, this workshop attracted 85 scientists from 13 countries, i.e. Austria, Brazil, Czechoslovakia, Fed. Rep. of Germany, France, Great Britain, Hungary, Japan, Norway, Sweden, The Netherlands, USA, Yugoslavia.
Download or read book Multiscale X Ray Analysis of Biological Cells and Tissues by Scanning Diffraction and Coherent Imaging written by Jan-David Nicolas and published by Göttingen University Press. This book was released on 2019 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the intricate details of muscle contraction has a long-standing tradition in biophysical research. X-ray diffraction has been one of the key techniques to resolve the nanometer-sized molecular machinery involved in force generation. Modern, powerful X-ray sources now provide billions of X-ray photons in time intervals as short as microseconds, enabling fast time-resolved experiments that shed further light on the complex relationship between muscle structure and function. Another approach harnesses this power by repeatedly performing such an experiment at different locations in a sample. With millions of repeated exposures in a single experiment, X-ray diffraction can seamlessly be turned into a raster imaging method, neatly combining real- and reciprocal space information. This thesis has focused on the advancement of this scanning scheme and its application to soft biological tissue, in particular muscle tissue. Special emphasis was placed on the extraction of meaningful, quantitative structural parameters such as the interfilament distance of the actomyosin lattice in cardiac muscle. The method was further adapted to image biological samples on a range of scales, from isolated cells to millimeter-sized tissue sections. Due to the ‘photon-hungry’ nature of the technique, its full potential is often exploited in combination with full-field imaging techniques. From the vast set of microscopic tools available, coherent full-field X-ray imaging has proven to be particularly useful. This multimodal approach allows to correlate two- and three-dimensional images of cells and tissue with diffraction maps of structure parameters. With the set of tools developed in this thesis, scanning X-ray diffraction can now be efficiently used for the structural analysis of soft biological tissues with overarching future applications in biophysical and biomedical research.
Download or read book Coherent X ray diffractive imaging on the single cell level of microbial samples written by Robin Niklas Wilke and published by Göttingen University Press. This book was released on 2015 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its first experimental demonstration in 1999, Coherent X-Ray Diffractive Imaging has become one of the most promising high resolution X-Ray imaging techniques using coherent radiation produced by brilliant synchrotron storage rings. The ability to directly invert diffraction data with the help of advanced algorithms has paved the way for microscopic investigations and wave-field analyses on the spatial scale of nanometres without the need for inefficient imaging lenses. X-Ray phase contrast which is a measure of the electron density is an important contrast mode of soft biological specimens. For the case of many dominant elements of soft biological matter, the electron density can be converted into an effective mass density offering a unique quantitative information channel which may shed light on important questions such as DNA compaction in the bacterial nucleoid through ‚weighing with light‘. In this work X-Ray phase contrast maps have been obtained from different biological samples by exploring different methods. In particular, the techniques Ptychography and Waveguide-Holographic-Imaging have been used to obtain twodimensional and three-dimensional mass density maps on the single-cell-level of freeze-dried cells of the bacteria Deinococcus radiodurans, Bacillus subtilis and Bacillus thuringiensis allowing, for instance, to estimate the dry weight of the bacterial genome in a near native state. On top of this, reciprocal space information from coherent small angle X-Ray scattering (cellular Nano-Diffraction) of the fine structure of the bacterial cells has been recorded in a synergistic manner and has been analysed down to a resolution of about 2.3/nm exceeding current limits of direct imaging approaches. Furthermore, the dynamic range of present detector technology being one of the major limiting factors of ptychographic phasing of farfield diffraction data has been significantly increased. Overcoming this problem for the case of the very intense X-Ray beam produced by Kirkpatrick-Baez mirrors has been explored by using semi-transparent central stops.
Download or read book Holography written by Fouad Sabry and published by One Billion Knowledgeable. This book was released on 2022-02-21 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: What Is Holography Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating three-dimensional images, but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. How You Will Benefit (I) Insights, and validations about the following topics: Chapter 1: Holography Chapter 2: Diffraction Chapter 3: Microscopy Chapter 4: Interferometry Chapter 5: Photorefractive effect Chapter 6: Particle image velocimetry Chapter 7: Holographic data storage Chapter 8: Interference lithography Chapter 9: Rainbow hologram Chapter 10: Holographic interferometry Chapter 11: Digital holography Chapter 12: Computer-generated holography Chapter 13: Volume hologram Chapter 14: Holographic display Chapter 15: Electronic speckle pattern interferometry Chapter 16: Speckle (interference) Chapter 17: Digital holographic microscopy Chapter 18: Holographic optical element Chapter 19: Common-path interferometer Chapter 20: Physics of optical holography Chapter 21: Time-domain holography (II) Answering the public top questions about holography. (III) Real world examples for the usage of holography in many fields. (IV) 17 appendices to explain, briefly, 266 emerging technologies in each industry to have 360-degree full understanding of holography' technologies. Who This Book Is For Professionals, undergraduate and graduate students, enthusiasts, hobbyists, and those who want to go beyond basic knowledge or information for any kind of holography.
Download or read book Lasers and Holography written by Prakash Chandra Mehta and published by World Scientific. This book was released on 1993 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive book on the fundamental aspects of lasers and holography. It represents a self-contained treatment of the basic and state-of-the-art topics on the two subjects in a single volume. The book covers the latest technological and scientific developments and major advances that have taken place in the last decade and includes recent applications in medicine, information processing, interconnects and nondestructive testing.It is intended to fill the need of both researchers and graduate students for a single source book containing the necessary and relevant material in the areas of lasers and holography.
Download or read book The State of the Laboratory written by and published by . This book was released on 1988 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.
