Download or read book What is Protein Misfolding Cyclic Amplification written by Dr. H. K. Saboowala. and published by Dr.Hakim Saboowala. This book was released on 2024-04-07 with total page 35 pages. Available in PDF, EPUB and Kindle. Book excerpt: What is Protein Misfolding Cyclic Amplification? Protein misfolding cyclic amplification (PMCA) is a technique that amplifies misfolded prions. It's used as a test for spongiform encephalopathies like: § Chronic wasting disease (CWD) or § Bovine spongiform encephalopathy (BSE). PMCA is similar to Polymerase Chain Reaction (PCR) but doesn't involve nucleotides. It's a widely used in vitro cell-free prion conversion assay. PMCA uses ultrasound waves to fragment the PrP(Sc) polymers, increasing the amount of seeds. PMCA is the sole technique that can successfully amplify the disease-associated, infectious form of the prion protein (PrPSc) in vitro. PMCA has been broadly applied in research areas that include the veterinary disease diagnosis. An attempt has been made in this E-Booklet to describe concisely the entire technique along with the relevant Images for better understanding at one click! Dr. H. K. Saboowala. M.B.(Bom) .M.R.S.H.(London)
Download or read book Optimization Adaptation and Application of Protein Misfolding Cyclic Amplification to Detection of Prions in Blood Plasma written by Shannon Lynn Braithwaite and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Serial Protein Misfolding Cyclic Amplification sPMCA to Detect Surrogate Markers for Chronic Wasting Disease in Surface Water Municipal Water and Soil written by Tracy A. Nichols and published by . This book was released on 2008 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Amyloid Proteins written by Einar M. Sigurdsson and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Generation and Propagation of Infectious Prions by Cyclic Amplification of Protein Misfolding written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Prion Diseases written by and published by Elsevier. This book was released on 2018-06-07 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Prion Diseases, Volume 153 is designed to update the reader on the latest advances and clinical aspects of prion diseases. The book is organized into five sections, including the pathophysiology of prions and a description of animal and human diseases. This is followed by detailed reports on recent advances in diagnosis strategies for the development of novel anti-prion molecules and possible designs of clinical trials in such a rare disease. An introductory chapter gives an extensive historical background of prion research, with a final chapter highlighting recent progress, and more importantly, unsolved problems. Offers an authoritative overview of prion diseases in humans, detailing the pathogenesis of the disease, clinical investigations, and the diagnosis of both the genetic and acquired forms Provides clarity and context by presenting prion diseases in relation to other neurodegenerative diseases in humans Emphasizes the unique properties of prion diseases and consequent problems they can cause, both clinically and in public health terms
Download or read book Alpha synuclein written by Tim Bartels and published by . This book was released on 2019 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Neuropathology of Neurodegenerative Diseases written by Gabor G. Kovacs and published by Cambridge University Press. This book was released on 2017-12-13 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical guide to the diagnosis of neurodegenerative diseases discusses modern molecular techniques, morphological classification, fundamentals of clinical symptomology, diagnostic pitfalls and immunostaining protocols. It is based on the proteinopathy concept of neurodegenerative disease, which has influenced classification and provides new strategies for therapy. Numerous high-quality images, including histopathology photomicrographs and neuroradiology scans, accompany the description of morphologic alterations and interpretation of immunoreactivities. Diagnostic methods and criteria are placed within recent developments in neuropathology, including the now widespread application of immunohistochemistry. To aid daily practice, the guide includes diagnostic algorithms and offers personal insights from experienced experts in the field. Special focus is given to the way brain tissue should be handled during diagnosis. This is a must-have reference for medical specialists and specialist medical trainees in the fields of pathology, neuropathology and neurology working with neuropathologic features of neurodegenerative diseases.
Download or read book Prion Biology written by Vincent Béringue and published by CRC Press. This book was released on 2013-04-08 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains a selection of chapters aimed to provide a better understanding prion structure and biology. Together these chapters provide an overview of prion biology and underscore some of the challenges we face if we want to understand how this lively pathogen propagates and evolves in mammals. There is also mounting evidence that studying prion biology has a wider relevance due to similarities in the processes of protein misfolding and aggregation between prion disorders and other neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington’s diseases.
Download or read book The Prion Protein written by Jorg Tatzelt and published by . This book was released on 2010 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: A conformational transition of the cellular prion protein (PrPC) into an aberrantly folded isoform designated scrapie prion protein (PrPSc) is the hallmark of a variety of neurodegenerative disorders collectively called prion diseases. They include Creutzfeldt-Jakob disease and Gerstmann-Stäussler-Scheinker syndrome in humans, scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle and chronic wasting disease (CWD) in free-ranging deer. In contrast to the deadly properties of misfolded PrP, PrPC seems to possess a neuroprotective activity. More-over, animal models indicated that the stress-protective activity of PrPC and the neurotoxic effects of PrPSc are somehow interconnected. In this timely book, leading scientists in the field have come together to highlight the apparently incongruous activities of different PrP conformers. The articles outline current research on celluar pathways implicated in the formation and signaling of neurotoxic and physiological PrP isoforms and delineate future research direction. Topics covered include the physiologcial activity of PrPC and its possible role as a neurotrophic factor, the finding that aberrant PrP conformers can cause neurodegeneration in the absence of infectious prion propagation, the requirement of the GPI anchor of PrPC for the neurotoxic effects of scrapie prions, the pathways implicated in the formation and neurotoxic properties of cytosolically localized PrP, the impact of metal ions on the processing of PrP, and the role of autophagy in the propagation and clearance of PrPSc. The book is fully illustrated and chapters include comprehensive reference sections. Essential reading for scientists involved in prion research.
Download or read book Prions written by Claudio Soto and published by CRC Press. This book was released on 2005-12-20 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prion-related diseases, known as transmissible spongiform encephalopathies (TSEs), are infectious, fatal neurodegenerative disorders for which there is no cure, treatment, nor even a means for early diagnosis. The horrific advent of Mad Cow Disease -- transmitted to humans through eating meat from steers sickened by bovine spongiform encephalopathy
Download or read book Ruminant Prion Disease Detection and Characterisation Using Protein Misfolding Cyclic Amplification written by Matthew O'Connor and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Prion Biology and Diseases written by Stanley B. Prusiner and published by CSHL Press. This book was released on 2004 with total page 1130 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a new edition of the most authoritative book on Prion Biology, first published in 1999 and edited by the Nobel Prize-winning founder of the field. This expanded edition has been completely updated, and includes chapters on therapeutics, and diagnostic methods and approaches.
Download or read book WHO Guidelines on Tissue Infectivity Distribution in Transmissible Spongiform Encephalopathies written by World Health Organization and published by . This book was released on 2006-01-01 with total page 53 pages. Available in PDF, EPUB and Kindle. Book excerpt: These guidelines provide evidence-based information to national regulatory authorities, especially to those where Bovine Spongiform Encephalopathy (BSE) has not yet been reported and where surveillance systems for BSE and variant Creutzfeldt-Jakob disease (vCJD), the human disease caused by infection with the BSE agent, may not be in place. Preventive measures to minimise the risk of transmitting TSEs to humans from pharmaceutical and biological products in which ruminant animal-derived materials are used and from humans to humans by blood and blood products and by human cells, tissues and organs are discussed.
Download or read book Neurodegenerative Diseases written by G. Jolles and published by Academic Press. This book was released on 1994-11-14 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last few years, the considerable progress made in biochemistry, virology, molecular biology and genetics has revealed some of the intimate mechanisms of the neurodegenerative diseases. The present volume is an attempt to review the latest data in the field to illuminate new avenues for future research. This volume gathers together chapters and discussions on the etiology and pathogenesis of the neurodegenerative diseases. Apoptosis of programmed cell death as well as other genetic implications are discussed; special attention is given to the coexistence and interconnection of genetic and environmental factors. There is extensive coverage of prions responsible for bovine spongiform encephalopathy, Cruetzfeld-Jacob disease and kuru. The various aspects of non-conventional transmissible agents are thoroughly reviewed. Further contributions deal with the role of growth factors as well as of free radicals. Consideration is given to the molecular mechanisms of Alzheimer's disease, in particular the role of tau protein. Finally, several pharmacological models now available, which throw light upon aspects of Parkinson's disease, Huntington's chorea and multiple sclerosis, are examined and discussed. It is hoped that recent scientific advances will lead to the discovery of new drugs to fill the current therapeutic void. There are hopes of an early indication of this in the case of amyotrophic lateral sclerosis.
Download or read book Prions and Diseases written by Wen-Quan Zou and published by Springer Nature. This book was released on 2023-01-01 with total page 773 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transmissible spongiform encephalopathies (TSE), known as prion diseases, have been recognized for nearly 300 years in animals and almost 100 years in humans. Modern studies, including the protein-misfolding cyclic amplification (PMCA), have greatly advanced our understanding of the pathogenesis of prion diseases and facilitated the identification of new prion diseases in animals and humans. In the second edition of Prions and Diseases, more than 60 leading researchers and clinicians worldwide provide an up-to-date discussion of these unique infectious pathogens and their associated diseases. The book provides up-to-date knowledge about the etiology, pathogenesis, classification, histopathological, and clinical aspects of the full range of animal and human prion diseases. As a result, the book contains by far the most authoritative views about the past, present, and future of prions and prion diseases. The new second edition covers such important emerging topics such as inherited human prion disease, stem-cell models in prion research, human prion disease surveillance, and gene therapy strategies.
Download or read book Techniques In Prion Research written by Sylvain Lehmann and published by Springer Science & Business Media. This book was released on 2004-11-22 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: -There is an acceleration in prion disease research because of the spread of mad cow disease. -This book covers in vitro, cellular, and animal models adapted for the study of TSEs. -Includes bio-saftey procedures.
