Download or read book Wavefront Sensing and High Resolution Adaptive Optics Imaging in the Living Rodent Eye written by Ying Geng and published by . This book was released on 2011 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The rodent has become an increasingly valuable model for human diseases and development due to its availability for genetic manipulations. Non-invasive microscopic imaging of the rodent retina would allow tracking of retinal development, disease progression, and the efficacy of therapy in single animals. Correction of the eye's aberrations using adaptive optics (AO) could improve the resolution of in vivo rodent retinal images, but previous attempts have been limited by the small size of its eye and the difficulty in measuring its aberrations due to poor Shack-Hartmann wavefront sensor (SHWS) spot quality. The work in this thesis describes methods developed to measure the rodent eye optics and to optimize its retinal image quality in vivo. Our first attempt was modifying a confocal fluorescence adaptive optics scanning laser ophthalmoscope (AOSLO) originally built for imaging the primate and human eye to accommodate the rat eye. Despite achieving in vivo resolution sufficient to resolve sub-cellular structures in fluorescent ganglion cells, problems were identified with aberration measurements and AO image quality. We then constructed a SHWS customized for the small mouse eye, and found a solution to the aberration measurement problem. The custom designed SHWS can favor light from a specific retinal layer and provide good wavefront spot quality. This wavefront sensor was incorporated into a confocal AOSLO custom designed for the mouse eye. High quality images were obtained in the mouse retina revealing multiple cell layers, including the photoreceptor mosaic, nerve fiber bundles, fine capillaries/blood flow, and ganglion cell bodies and fine processes. The in vivo resolution of the system was directly characterized to be sub-[micrometer] laterally, and ~10 [micrometers] axially. This fine resolution has allowed classification of ganglion cells in vivo. The value of the instrument was also demonstrated in two functional imaging scientific studies"--Leaves vii-viii.
Download or read book Adaptive Optics Optical Coherence Tomography for in Vivo Retinal Imaging written by Yifan Jian and published by . This book was released on 2014 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical coherence tomography (OCT) is a non-invasive micrometer-resolution volumetric imaging modality that has been employed in diverse applications. In this thesis, we first describe a GPU accelerated program to perform FDOCT data processing and real time 3D volumetric rendering. The real time visualization of volumetric images provided by the GPU acceleration was essential to the rest of the work described in this thesis. Small animal models of retinal diseases serve as a vital component in vision research, and non-invasive in vivo imaging is becoming an increasingly important tool in the field. We describe the first adaptive optics optical coherence tomography (AOOCT) imaging system for high resolution mouse retinal imaging. Images of mouse retina acquired with AOOCT showed significant improvement in the brightness and contrast of capillaries and nerve fiber bundles. However, the accuracy of wavefront sensing limited the performance of AOOCT. A novel wavefront sensorless adaptive optics (WSAO) OCT system was developed to overcome the issues associated with conventional wavefront sensing. Combination of WSAO with OCT allows coherence gated, depth resolved aberration correction. Images of both pigmented and albino mouse retinas acquired using WSAO OCT system demonstrated superior image quality. The real time high resolution WSAO OCT was leveraged for a time course study of laser exposure in the retina.
Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Download or read book Adaptive Optics with a Micromachined Membrane Deformable Mirror for High Resolution Retinal Imaging written by Lijun Zhu and published by . This book was released on 1999 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Wavefront Shaping for Biomedical Imaging written by Joel Kubby and published by Cambridge University Press. This book was released on 2019-06-20 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn about the theory, techniques and applications of wavefront shaping in biomedical imaging using this unique text. With authoritative contributions from researchers who are defining the field, cutting-edge theory is combined with real-world practical examples, experimental data and the latest research trends to provide the first book-level treatment of the subject. It is suitable for both background reading and use in a course, with coverage of essential topics such as adaptive optical microscopy, deep tissue microscopy, time reversal and optical phase conjugation, and tomography. The latest images from the forefront of biomedical imaging are included, and full-colour versions are available in the eBook version. Researchers, practitioners and graduate students in optics, biophotonics, biomedical engineering, and biology who use biomedical imaging tools and are looking to advance their knowledge of the subject will find this an indispensable resource.
Download or read book Advanced Biophotonics written by Ruikang K. Wang and published by Taylor & Francis. This book was released on 2016-04-19 with total page 726 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and
Download or read book Imaging Single cell Dynamics in the Living Eye from Milliseconds to Months written by Aby Joseph and published by . This book was released on 2021 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Advances in microscopy have focused on improvements in both the spatial and temporal dimensions. In the spatial dimension, adaptive optics ophthalmoscopy has given us single-cell resolution in the eye by turning the living eye into a microscope, enabling noninvasive structural imaging of the retina with ~1 um in vivo lateral resolution. However, a snapshot of structure alone does not give a complete picture of cell function. One way to study function in the eye is to image changes in cells over time. Depending on the function of interest, changes can be tracked over a broad range of time-scales. Here I combine high spatial resolution adaptive optics imaging with 1) ultrafast imaging (for short time-scales) and 2) time-lapse imaging (for long time-scales) to investigate cellular function in the living retina, a part of the central nervous system. All imaging is performed noninvasively in mice, using near-infrared light, allowing for longitudinal imaging of the same cellular-scale retinal location. Imaging and computational innovations are applied to study, in health and disease, two support systems of neurons: the vascular and immune systems. In the healthy vascular system, single-cell blood flow is quantitatively imaged in the smallest to largest vessels of the mammalian retina, over milliseconds to minutes. In the healthy immune system, dynamics of white blood cells (circulating immune cells) and single-process motility of microglia (resident immune cells) are studied, over milliseconds to months. Finally, the imaging techniques characterized in health are applied to an inflammatory disease, where both the individual responses and the interplay between the vascular and immune systems are studied. In a subset of experiments, transgenic mice with fluorescently labelled cells are used to confirm cell-types imaged; with careful correction of the high transverse chromatic aberration in the mouse eye. Finally, pilot studies are performed on awake-behaving mice and human beings to implement some of the noninvasive strategies developed in the anesthetized mouse"--Page xi.
Download or read book Handbook of Visual Optics Volume Two written by Pablo Artal and published by CRC Press. This book was released on 2017-02-24 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Visual Optics offers an authoritative overview of encyclopedic knowledge in the field of physiological optics. It builds from fundamental concepts to the science and technology of instruments and practical procedures of vision correction, integrating expert knowledge from physics, medicine, biology, psychology, and engineering. The chapters comprehensively cover all aspects of modern study and practice, from optical principles and optics of the eye and retina to novel ophthalmic tools for imaging and visual testing, devices and techniques for visual correction, and the relationship between ocular optics and visual perception.
Download or read book The Design of High speed and Multi scale in Vivo Retinal Imaging with Wavefront Sensorless Adaptive Optics Optical Coherence Tomography written by Ji Hoon Kwon and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human retinal imaging is rapidly growing industry due to the special characteristics of the eye which has irreversible vision-robbing diseases. Optical Coherence Tomography (OCT) is routinely applied for retinal imaging since it provides three-dimensional retinal images non-invasively. With advancements of the technology, current commercial OCT systems in clinics are able to zoom in and out the region of interest, visualizing and acquiring various features of the retina image in several seconds. However, still there are limitations of achievable imaging angle, image quality and acquisition speed that need to be improved. In this thesis, we introduce the multi-scale feature, adjusting the beam diameter (and hence the resolution) to match each field-of-view to improve the image quality. Furthermore, this system employed a recently developed light source which provides much faster imaging speed which is critical to minimize motion artifacts, providing various potential to enhance the image quality.
Download or read book Functional Imaging and Classification of Retinal Ganglion Cells in the Living Primate Eye written by Tyler Christian Godat and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The primate fovea is perhaps the most important location for vision in the retina, being specialized for high resolution spatial and chromatic vision, but it has been traditionally difficult to study in ex vivo electrophysiology and especially in vivo. The crucial visual properties conferred by the fovea are mediated by neurons called retinal ganglion cells, which exist in twenty or more distinct types that all tile the retina and are present from the far periphery up to the inner edge of the foveal slope. Microelectrode recordings of foveal retinal ganglion cells have been challenging due to their reduced size and the fragility of tissue survival in excised retina. Some of these difficulties in observing individual cells have been overcome in the in vivo retina by combining high resolution fluorescence adaptive optics ophthalmoscopy with calcium imaging to optically record functional responses in the living primate eye. The work in this thesis advances knowledge of functional properties of retinal ganglion cells at the primate fovea by studying their responses to various stimuli in the living primate (macaca fascicularis) eye. This includes measurements of retinal ganglion cells' chromatic tuning and cone weightings, made possible by silent substitution style uniform chromatic flicker stimuli, and measurements of cells' spatial transfer functions, made possible by monochromatic drifting gratings of various spatial frequencies. In particular, the most common type of retinal ganglion cell, the midget, is studied extensively to generate a broader understanding of the specialization and importance of the primate fovea. This work thus provides a closer look at the in situ spatial, chromatic, and temporal properties of these retinal ganglion cells than ever before possible. A novel stimulation delivery arm as part of a dual adaptive optics ophthalmoscope is also developed as part of this work to improve the resolution and breadth of stimuli used to interrogate the functional properties of retinal ganglion cells. These imaging and stimulation techniques provide a suite of tools for assessing and characterizing the functional properties of retinal ganglion cells in vivo which will inform the next generation of vision restoration strategies such as optogenetics and retinal prostheses."--Pages xi-xii.
Download or read book Optical Coherence Tomography written by Wolfgang Drexler and published by Springer Science & Business Media. This book was released on 2008-12-10 with total page 1346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical coherence tomography (OCT) is the optical analog of ultrasound imaging and is emerging as a powerful imaging technique that enables non-invasive, in vivo, high resolution, cross-sectional imaging in biological tissue. This book introduces OCT technology and applications not only from an optical and technological viewpoint, but also from biomedical and clinical perspectives. The chapters are written by leading research groups, in a style comprehensible to a broad audience.
Download or read book Multimodal Imaging in Uveitis written by H. Nida Sen and published by Springer. This book was released on 2017-12-09 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive guide to the imaging techniques that have revolutionized the diagnosis and management of uveitis during the past decade, including optical coherence tomography (OCT), enhanced depth imaging, fundus autofluorescence, and wide-field angiography. In addition, the current role of the traditional (invasive) gold standard techniques, fluorescein angiography and indocyanine green angiography, is described. Among the newer imaging modalities, detailed attention is paid to the various OCT technologies such as spectral domain OCT, enhanced-depth imaging OCT, and enface swept-source OCT. Further individual chapters focus on imaging using adaptive optics, multiview OCT, and OCT angiography. Uveitis can affect virtually any structure in the eye, and imaging of these structures is critical in the diagnosis, prognosis, and management of the disease. Increasing use and better understanding of the different modalities described in this book are sure to improve our knowledge of disease mechanisms and likely outcomes.
Download or read book Autofluorescence Imaging of Retinal Pigment Epithelial Cells in the Living Human Eye written by Charles E. Granger and published by . This book was released on 2020 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The lens and cornea of the eye enable vision by focusing images of the world on the light-sensitive retina. In turn, they can be conveniently utilized as an objective lens, allowing visualization and imaging of the retina in ophthalmoscopy. This technique is used every day to non-invasively diagnose and monitor retinal disease and injury, to allow retinal surgery, and study fundamentals of vision science. The implementation of adaptive optics technology further enables ophthalmoscopy by measuring and correcting the eye's aberrations and allowing high-resolution imaging of retinal cells in the living eye. Cellular-scale in vivo imaging has provided greater insight into the study of disease, physiology, and even allowed advanced techniques for probing neuronal function. Despite the resolution improvement, many retinal cells critical to vision remain difficult to image with traditional reflected light, requiring the implementation and development of advanced microscopy imaging techniques where possible. One such layer of cells is the retinal pigment epithelium (RPE), which maintains the health and normal function of rod and cone photoreceptors, and is affected in many retinal diseases that cause blindness. While these cells cannot be visualized using standard reflectance imaging, they are unique from other retinal cells in that they contain relatively strong endogenous fluorophores, namely lipofuscin and melanin. The work in this thesis aims to advance methods of autofluorescence imaging of the RPE in the living human eye through adaptive optics ophthalmoscopy. This includes single-photon fluorescence intensity imaging techniques, utilizing both visible and near-infrared excitation, allowing for structural analysis of cells in human subjects. Structural analysis is then complemented by the implementation of fluorescence lifetime imaging, potentially providing functional analysis of cells and a more effective method of assessing cell health. These imaging techniques provide a set of tools for characterizing and studying RPE cells with structural and functional biomarkers in both healthy and diseased human eyes"--Pages xiii-xiv.
Download or read book High resolution Retinal Imaging with a Compact Adaptive Optics Ophthalmoscope written by Ibolya Edit Kepiro and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Visual Optics Volume One written by Pablo Artal and published by CRC Press. This book was released on 2017-02-17 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Visual Optics offers an authoritative overview of encyclopedic knowledge in the field of physiological optics. It builds from fundamental concepts to the science and technology of instruments and practical procedures of vision correction, integrating expert knowledge from physics, medicine, biology, psychology, and engineering. The chapters comprehensively cover all aspects of modern study and practice, from optical principles and optics of the eye and retina to novel ophthalmic tools for imaging and visual testing, devices and techniques for visual correction, and the relationship between ocular optics and visual perception.
Download or read book Progress in Adaptive Optics for High Resolution Imaging written by Simon Tuohy and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Color Vision written by Jan Kremers and published by Springer. This book was released on 2016-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our understanding of human color vision has advanced tremendously in recent years, helped along by many new discoveries, ideas, and achievements. It is therefore timely that these new developments are brought together in a book, assembled specifically to include new research and insight from the leaders in the field. Although intentionally not exhaustive, many aspects of color vision are discussed in this Springer Series in Vision Research book including: the genetics of the photopigments; the anatomy and physiology of photoreceptors, retinal and cortical pathways; color perception; the effects of disorders; theories on neuronal processes and the evolution of human color vision. Several of the chapters describe new, state-of-the-art methods within genetics, morphology, imaging techniques, electrophysiology, psychophysics, and computational neuroscience. The book gives a comprehensive overview of the different disciplines in human color vision in a way that makes it accessible to specialists and non-specialist scientists alike. About the Series: The Springer Series in Vision Research is a comprehensive update and overview of cutting edge vision research, exploring, in depth, current breakthroughs at a conceptual level. It details the whole visual system, from molecular processes to anatomy, physiology and behavior and covers both invertebrate and vertebrate organisms from terrestrial and aquatic habitats. Each book in the Series is aimed at all individuals with interests in vision including advanced graduate students, post-doctoral researchers, established vision scientists and clinical investigators. The series editors are N. Justin Marshall, Queensland Brain Institute, The University of Queensland, Australia and Shaun P. Collin, Neuroecology Group within the School of Animal Biology and the Oceans Institute at the University of Western Australia.
