Download or read book Voltage and Patch Clamping with Microelectrodes written by Thomas G Smith and published by Springer. This book was released on 2013-05-27 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edited and authored by international experts on voltage and patch clamping, this volume is designed to help anyone undertaking experiments requiring the use of these techniques. The only book of its kind to bring together this wealth of information on theory as well as practical techniques, this is a volume that no one involved in voltage and patch clamping can afford to be without.
Download or read book Patch Clamping written by Areles Molleman and published by John Wiley & Sons. This book was released on 2003-06-13 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
Download or read book Patch Clamp Analysis written by Wolfgang Walz and published by Springer Science & Business Media. This book was released on 2008-02-06 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
Download or read book Patch Clamp Techniques written by Yasunobu Okada and published by Springer. This book was released on 2012-04-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Download or read book Microelectrode Techniques written by David Ogden and published by . This book was released on 1994 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Channel Recording written by Bert Sakmann and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.
Download or read book Manual of Research Techniques in Cardiovascular Medicine written by Hossein Ardehali and published by John Wiley & Sons. This book was released on 2013-12-16 with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt: While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical “how-to-do-it” review of best-practice techniques for cardiovascular research.
Download or read book Practical Methods in Cardiovascular Research written by Stefan Dhein and published by Springer. This book was released on 2006-02-28 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.
Download or read book Dynamic Clamp written by Alain Destexhe and published by Springer Science & Business Media. This book was released on 2009-03-11 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dynamic-clamp is a fascinating electrophysiology technique that consists of merging living neurons with computational models. The dynamic-clamp (also called “conductance injection”) allows experimentalists and theoreticians to challenge neurons (or any other type of cell) with complex conductance stimuli generated by a computer. The technique can be implemented from neural simulation environments and a variety of custom-made or commercial systems. The real-time interaction between the computer and cell also enables the design of recording paradigms with unprecedented accuracy via a computational model of the electrode. Dynamic-Clamp: From Principles to Applications contains contributions from leading researchers in the field, who investigate these paradigms at the cellular or network level, in vivo and in vitro, and in different brain regions and cardiac cells. Topics discussed include the addition of artificially-generated synaptic activity to neurons; adding, amplifying or neutralizing voltage-dependent conductances; creating hybrid networks with real and artificial cells; attaching simulated dendritic tree structures to the living cell; and connecting different neurons. This book will be of interest to experimental biophysicists, neurophysiologists, and cardiac physiologists, as well as theoreticians, engineers, and computational neuroscientists. Graduate and undergraduate students will also find up-to-date coverage of physiological problems and how they are investigated.
Download or read book Patch Clamp Electrophysiology written by Mark Dallas and published by Humana. This book was released on 2021-11-13 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.
Download or read book Gigaseal Formation in Patch Clamping written by Majid Malboubi and published by Springer Science & Business Media. This book was released on 2013-07-24 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
Download or read book Ion Channels written by Nikita Gamper and published by Humana Press. This book was released on 2016-05-01 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology book offers strategies and protocols for studying a large group of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Includes step-by-step protocols, materials lists, tips and more."
Download or read book Microelectrode Techniques written by Nick B. Standen and published by . This book was released on 1987 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Physiology Source Book written by Nicholas Sperelakis and published by Elsevier. This book was released on 2012-12-02 with total page 1262 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative book gathers together a broad range of ideas and topics that define the field. It provides clear, concise, and comprehensive coverage of all aspects of cellular physiology from fundamental concepts to more advanced topics. The Third Edition contains substantial new material. Most chapters have been thoroughly reworked. The book includes chapters on important topics such as sensory transduction, the physiology of protozoa and bacteria, the regulation of cell division, and programmed cell death. Completely revised and updated - includes 8 new chapters on such topics as membrane structure, intracellular chloride regulation, transport, sensory receptors, pressure, and olfactory/taste receptors Includes broad coverage of both animal and plant cells Appendixes review basics of the propagation of action potentials, electricity, and cable properties Authored by leading experts in the field Clear, concise, comprehensive coverage of all aspects of cellular physiology from fundamental concepts to more advanced topics
Download or read book Ion Channels of Excitable Cells written by Toshio Narahashi and published by . This book was released on 1994 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because of the highly significant and widely recognized roles of ion channels in physiology, pathophysiology, pharmacology, and toxicology, the term ion channel has now become a household word in the biomedical sciences. This volume covers preparations and techniques for the study of various ion channels. Both voltage-gated and ligand-gated ion channels of neurons, axons, and cardiac and smooth muscles are covered. It includes not only patch clamp techniques but molecular biology and imaging techniques as well. Key Features * Comprehensive protocols included for the study of: * Ion channels using patch-clamp, molecular biology, and imaging techniques * Role of ion channels in physiology, pathophysiology, pharmacology, and toxicology * Specific ion channels of specific tissues
Download or read book Basic Electrophysiological Methods written by Ellen Covey and published by Oxford University Press. This book was released on 2015-02-25 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.
Download or read book Introduction to Epilepsy written by Gonzalo Alarcón and published by Cambridge University Press. This book was released on 2012-04-26 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers all aspects of epilepsy, from basic mechanisms to diagnosis and management, as well as legal and social considerations.
