Download or read book Vectorial Transport of Proteins into and across Membranes written by and published by Academic Press. This book was released on 1991-10-11 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third volume in this series covering protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport, Parts A and B, treated techniques for eukaryotic cells available as of 1989. Vectorial Transport of Proteins into and across Membranes, brings together new methods and new topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions, transcription, crosslinking, pulse-labeling, and reconstitution. Describes contemporary approaches for studying protein transport, protein topology and organelle biogenesis Covers methods from pre-eminent laboratories including: In vitro and in vivo investigations; Biochemical and immunological techniques; Prokaryote, chloroplast, mitochondria, peroxisome, and rough endoplasmic reticulum membranes
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory Methods in Vesicular and Vectorial Transport written by Alan Tartakoff and published by Academic Press. This book was released on 2012-12-02 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Methods in Vesicular and Vectorial Transport describes the procedures used to study the mechanisms of vesicular transport along the secretory and endocytic paths, including electron microscopy, autoradiography, and methods associated with cyto- and immunocytochemistry, genetics, and biochemistry. It investigates vectorial transport to the cisternal space of the rough endoplasmic reticulum (ER), as well as protein translocation across the ER, strategies for gaining access to the cytoplasm, cell-free analysis of vesicle fusion, the structure of glycoproteins, and the use of cell systems for analysis of vesicular traffic. Organized into seven parts encompassing 20 chapters, this volume begins with an overview of protein topology in the ER and the use of cross-linking methods to probe the molecular environment of translocating polypeptide chains. It then discusses the reconstitution of secretory protein translocation from detergent-solubilized rough microsomes; the use of anti-idiotype antibodies to characterize protein-protein interactions; the use of perforated cells to elucidate intracellular membrane transport; delivery of macromolecules into cells expressing a viral membrane fusion protein; and digitonin permeabilization procedures for studying endosome acidification and function. The reader is also introduced to reconstitution of intracellular vesicle fusion in a cell-free system after receptor-mediated endocytosis; immunoisolation using magnetic solid supports; endosome and lysosome purification by free-flow electrophoresis; remodeling of glycoprotein oligosaccharides after endocytosis; and replica plating of animal cells. This book will interest students, researchers, geneticists, biochemists, and cell biologists.
Download or read book Membrane Transporters and Channels as Targets for Drugs written by Graça Soveral and published by Frontiers Media SA. This book was released on 2020-01-03 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transporters and channels are membrane proteins that mediate the traffic of metabolites, water and ions across biological membranes. Membrane transport proteins are crucial to maintain homeostasis and assure cell survival upon intracellular or environmental stress. A failure of any of these transport systems may have dramatic consequences for cell function. There is increasing evidence that membrane transport proteins play important functions in healthy conditions and that their absence or dysfunction may cause diseases. In recent years much attention has been paid to diseases resulting from defective transporters (“carrier diseases”) and ion channels (“channelopathies”). Very interestingly, altered expression of transporters has been described in several human pathologies. On this basis, many transport proteins are well acknowledged targets for drugs. Many others are involved in drug delivery and disposition and/or are considered potential targets. Others are off-targets for drugs and then, are responsible for side effects. Thus, membrane protein drug discovery is now an emerging field where the search for physiological mechanisms of regulation and for chemical compounds as modulators of transport activity, present new opportunities for drug development and for new therapies. This Research Topic addresses the latest research advances in membrane transport proteins, stimulating future research on these important protein families.
Download or read book Transport And Diffusion Across Cell Membranes written by Wilfred Stein and published by Elsevier. This book was released on 2012-12-02 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transport and Diffusion across Cell Membranes is a comprehensive treatment of the transport and diffusion of molecules and ions across cell membranes. This book shows that the same kinetic equations (with appropriate modification) can describe all the specialized membrane transport systems: the pores, the carriers, and the two classes of pumps. The kinetic formalism is developed step by step and the features that make a system effective in carrying out its biological role are highlighted. This book is organized into six chapters and begins with an introduction to the structure and dynamics of cell membranes, followed by a discussion on how the membrane acts as a barrier to the transmembrane diffusion of molecules and ions. The following chapters focus on the role of the membrane's protein components in facilitating transmembrane diffusion of specific molecules and ions, measurements of diffusion through pores and the kinetics of diffusion, and the structure of such pores and their biological regulation. This book methodically introduces the reader to the carriers of cell membranes, the kinetics of facilitated diffusion, and cotransport systems. The primary active transport systems are considered, emphasizing the pumping of an ion (sodium, potassium, calcium, or proton) against its electrochemical gradient during the coupled progress of a chemical reaction while a conformational change of the pump enzyme takes place. This book is of interest to advanced undergraduate students, as well as to graduate students and researchers in biochemistry, physiology, pharmacology, and biophysics.
Download or read book Vectorial Transport of Proteins into and across Membranes written by and published by Academic Press. This book was released on 1991-09-06 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third volume in this series covering protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport, Parts A and B, treated techniques for eukaryotic cells available as of 1989. Vectorial Transport of Proteins into and across Membranes, brings together new methods and new topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions, transcription, crosslinking, pulse-labeling, and reconstitution.
Download or read book Protein Movement Across Membranes written by Jerry Eichler and published by Springer. This book was released on 2011-02-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the most recent advances in the transport of proteins across a variety of biological membranes. In addressing this topic, this volume includes several new twists not previously addressed in the literature. In the last few years, the study of protein translocation has been revolutionized by the availability of structural information on many of the components and complexes involved in the process. Unlike earlier books written on protein translocation, this volume considers these advances. In addition, several chapters discuss facets of protein translocation from a systems biology perspective, considered by many to be the next paradigm for biological study. Readers of this book will come away with a deeper understanding of the problems facing researchers of protein translocation and see how the most modern biological techniques and approaches are being recruited to answer those questions. The chapters are also written such that problems awaiting future investigation are clearly presented.
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Drug Transporters written by Martin F. Fromm and published by Springer Science & Business Media. This book was released on 2010-11-19 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is increasingly recognized that various transporter proteins are expressed throughout the body and determine absorption, tissue distribution, biliary and renal elimination of endogenous compounds and drugs and drug effects. This book will give an overview on the transporter families which are most important for drug therapy. Most chapters will focus on one transporter family highlighting tissue expression, substrates, inhibitors, knock-out mouse models and clinical studies.
Download or read book An Introduction to Biological Membranes written by William Stillwell and published by Newnes. This book was released on 2013-04-20 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable manner Emphasis on the historical development of the field Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport
Download or read book Encyclopedia of Molecular Pharmacology written by Stefan Offermanns and published by Springer Science & Business Media. This book was released on 2008-08-14 with total page 1515 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential text, this is a fully updated second edition of a classic, now in two volumes. It provides rapid access to information on molecular pharmacology for research scientists, clinicians and advanced students. With the A-Z format of over 2,000 entries, around 350 authors provide a complete reference to the area of molecular pharmacology. The book combines the knowledge of classic pharmacology with the more recent approach of the precise analysis of the molecular mechanisms by which drugs exert their effects. Short keyword entries define common acronyms, terms and phrases. In addition, detailed essays provide in-depth information on drugs, cellular processes, molecular targets, techniques, molecular mechanisms, and general principles.
Download or read book Protein Targeting to Mitochondria written by F.U. Hartl and published by Elsevier. This book was released on 1997-02-24 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: A large number of newly-synthesized polypeptides must cross one or several intracellular membranes to reach their functional locations in the eukaryotic cell. The mechanisms of protein trafficking, in particular the post-translational targeting and membrane translocation of proteins, are of fundamental biological importance and are the focus of intensive research world-wide. For more than 15 years, mitochondria have served as the paradigm organelle system to study these processes. Although key questions, such as how precisely proteins cross a membrane, still remain to be answered, exciting progress has been made in understanding the basic pathways of protein import into mitochondria and the components involved. In addition to a fascinating richness and complexity in detail, the analysis of mitochondrial protein import has revealed mechanistic principles of general significance: Major discoveries include the demonstration of the requirement of an unfolded state for translocation and of the essential role of molecular chaperones on both sides of the membranes in maintaining a translocation-competent conformation and in protein folding after import. It is becoming clear how a polypeptide chain is "reeled" across the membrane in an ATP-dependent process by the functional cooperation of membrane proteins, presumably constituting part of a transmembrane channel, with peripheral components at the trans-side of the membrane.In this volume, eminent experts in the field take the time to review the central aspects of mitochondrial biogenesis. The logical order of the 16 chapters is determined by the sequence of steps during protein import, starting with the events taking place in the cytosol, followed by the recognition of targeting signals, the translocation of precursor proteins across the outer and inner membranes, their proteolytic processing and intramitochondrial sorting, and finally their folding and oligomeric assembly. In addition, the mechanisms involved in the export of mitochondrially encoded proteins as well as recent advances in understanding the division and inheritance of mitochondria will be discussed.
Download or read book Plant ABC Transporters written by Markus Geisler and published by Springer. This book was released on 2014-09-06 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is devoted to the fascinating superfamily of plant ATP-binding cassette (ABC) transporters and their variety of transported substrates. It highlights their exciting biological functions, covering aspects ranging from cellular detoxification, through development, to symbiosis and defense. Moreover, it also includes a number of chapters that center on ABC transporters from non-Arabidopsis species. ABC proteins are ubiquitous, membrane-intrinsic transporters that catalyze the primary (ATP-dependent) movement of their substrates through biological membranes. Initially identified as an essential aspect of a vacuolar detoxification process, genetic work in the last decade has revealed an unexpectedly diverse variety of ABC transporter substrates, which include not only xenobiotic conjugates, but also heavy metals, lipids, terpenoids, lignols, alkaloids and organic acids. The discovery that members of the ABCB and ABCG family are involved in the movement of phytohormones has further sparked their exploration and provided a new understanding of the whole family. Accordingly, the trafficking, regulation and structure-function of ABCB-type auxin transporters are especially emphasized in this book.
Download or read book Plant Aquaporins written by François Chaumont and published by Springer. This book was released on 2017-02-07 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aquaporins are channel proteins that facilitate the diffusion of water and small uncharged solutes across cellular membranes. Plant aquaporins form a large family of highly divergent proteins that are involved in many different physiological processes. This book will summarize the recent advances regarding plant aquaporins, their phylogeny, structure, substrate specificity, mechanisms of regulation and roles in various important physiological processes related to the control of water flow and small solute distribution at the cell, tissue and plant level in an ever-changing environment.
Download or read book The Liver written by Irwin M. Arias and published by John Wiley & Sons. This book was released on 2020-03-09 with total page 1156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bridging the gap between basic scientific advances and the understanding of liver disease — the extensively revised new edition of the premier text in the field. The latest edition of The Liver: Biology and Pathobiology remains a definitive volume in the field of hepatology, relating advances in biomedical sciences and engineering to understanding of liver structure, function, and disease pathology and treatment. Contributions from leading researchers examine the cell biology of the liver, the pathobiology of liver disease, the liver’s growth, regeneration, metabolic functions, and more. Now in its sixth edition, this classic text has been exhaustively revised to reflect new discoveries in biology and their influence on diagnosing, managing, and preventing liver disease. Seventy new chapters — including substantial original sections on liver cancer and groundbreaking advances that will have significant impact on hepatology — provide comprehensive, fully up-to-date coverage of both the current state and future direction of hepatology. Topics include liver RNA structure and function, gene editing, single-cell and single-molecule genomic analyses, the molecular biology of hepatitis, drug interactions and engineered drug design, and liver disease mechanisms and therapies. Edited by globally-recognized experts in the field, this authoritative volume: Relates molecular physiology to understanding disease pathology and treatment Links the science and pathology of the liver to practical clinical applications Features 16 new “Horizons” chapters that explore new and emerging science and technology Includes plentiful full-color illustrations and figures The Liver: Biology and Pathobiology, Sixth Edition is an indispensable resource for practicing and trainee hepatologists, gastroenterologists, hepatobiliary and liver transplant surgeons, and researchers and scientists in areas including hepatology, cell and molecular biology, virology, and drug metabolism.
Download or read book The Membranes of Cells written by Philip Yeagle and published by . This book was released on 1993 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition of The Membranes of Cells, all of the chapters have been updated, some have been completely rewritten, and a new chapter on receptors has been added. The book has been designed to provide both the student and researcher with a synthesis of information from a number of scientific disciplines to create a comprehensive view of the structure and function of the membranes of cells. The topics are treated in sufficient depth to provide an entry point to the more detailed literature needed by the researcher. Key Features * Introduces biologists to membrane structure and physical chemistry * Introduces biophysicists to biological membrane function * Provides a comprehensive view of cell membranes to students, either as a necessary background for other specialized disciplines or as an entry into the field of biological membrane research * Clarifies ambiguities in the field
Download or read book Biomembranes written by Lionel A. Manson and published by Springer. This book was released on 1971-01-10 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the contributions to a symposium held at Gatlinburg, Tennessee, under the auspices of the Oak Ridge National Laboratory, in April, 1971. In the past, these proceedings had appeared as a supplement to the Journal of Cellular Physiology. Due to the nature of the subject ma terial and the relevance of the topic of the symposium to the readers of BIOMEMBRANES, it was agreed by the organizers of the symposium to publish the contributions of the partici pants as a separate volume in BIOMEMBRANES. It had been originally envisaged that, from time to time, the proceedings of a conference whose subject matter was directly related to the scope of this series would be included. The proceedings are being published exactly as they have been submitted to the Editor without the usual editorial re V1S10n. This is being done to increase the speed of publica tion. For the same rOeason, no indices have been provided since the time needed to prepare an adequate subject index would have unnecessarily delayed publication. Included in the proceedings are short reports of a number of workshops that were held during the conference. The editor has received excellent cooperation from both the organizers of the conference and the several contributors to this volume. If the experiment is a success, it is thanks to their promptness.
