Download or read book Use of Stream Biofilm Microbial Communities and Associated Metals as Indicators of Urban Runoff Impact on Freshwater Ecosystems written by Pierre-Yves Ancion and published by . This book was released on 2010 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The main focus of this project was to investigate how anthropogenic activities affect stream biofilm microbial communities and to assess the potential uses of biofilms as indicators of freshwater ecosystem health. The work improves our understanding of the impact of urbanisation on stream ecosystems and provides innovative techniques to assess this impact more accurately. Stream biofilms are the complex aggregation of microorganisms, covering almost every surface in freshwater environments. They play key roles in stream ecosystems and are grazed by many other organisms. Therefore, the potential impact of urbanisation on biofilm microbial communities could alter the functions of biofilms and affect the whole stream ecosystem. A wide range of experiments was conducted in both flow chamber microcosms and natural stream environments to investigate the impact of urban runoff on microbial community structure and composition. Using community fingerprinting techniques such as Terminal-Restriction Fragment Length Polymorphism and Automated Ribosomal Intergenic Spacer Analysis, as well as sequencing of bacterial 16S rRNA genes, we investigated changes occurring in biofilm bacterial and ciliate protozoan communities under exposure to urban contaminants. Flow chamber experiments revealed that significant differences in the structure of the microbial community could be detected within only a few days of exposure to urban runoff contaminants and remained detectable weeks after transfer to uncontaminated water. The rapid and persistent changes highlight the sensitivity of microbial communities and suggest that natural biofilm communities frequently exposed to urban runoff are significantly altered. Experiments carried out in natural streams confirmed the differences in composition and tolerance to contaminants between biofilm communities from preserved and impacted streams, suggesting critical implications for the whole stream ecosystem. The structure and composition of microbial communities associated with biofilms result from the influence of past and present environmental conditions and therefore constitute potential integrative indicators of stream health. Rapid changes in bacterial and ciliate community structure during exposure to metal contaminants demonstrated the sensitivity of these communities and their potential use as indicators of the influence of urban areas on stream ecosystem. Additional investigation conducted in natural streams indicated that bacterial and ciliate communities can reveal changes occurring along an urbanisation gradient and confirmed their reliable use as ecological bio-indicators. These novel indicators were implemented successfully to investigate the efficiency of an enclosed stormwater treatment system where traditional biological indicators such as benthic macroinvertebrates are not available. Results revealed the successful improvement of water through the treatment train and the minimal impact on the receiving creek. As biofilms are sessile structures, they may also accumulate urban contaminants and could form a critical link in the movement of urban contaminants from abiotic to biotic components of the stream. Therefore, the absorption and release rates of metals (Zn, Cu and Pb) in biofilms were investigated. Results revealed the fast accumulation of metals in the matrix during the first few days. High enrichment factors were recorded between the biofilm wet weight and the water, reaching 500:1 for zinc, 1500:1 for copper and 6000:1 for lead after 21 days of exposure. During recovery in uncontaminated water, metals were retained in the biofilm and 10 to 16 % of zinc and copper accumulated during exposure still remained in the biofilm after 14 days of recovery. The release of lead was even slower and more than 35 % of accumulated lead remained in the biofilm after 14 days of recovery. Investigation in natural streams confirmed the accumulation of metals in biofilms from urban streams and highlighted the potential risk for organisms at a higher trophic level. These results suggested that metals associated with biofilms could provide a highly relevant indicator of the presence of metals in freshwater systems at concentrations detrimental to aquatic biota. Using biofilm bacterial, ciliate and macro-invertebrate communities as bio-indicators, the relevance of biofilm associated metals (As, Cd, Cr, Cu, Pb, Ni and Zn) was investigated and compared to sediment associated metals. A greater proportion of the changes occurring in each of the communities could be explained by metal concentrations in biofilm than in sediments confirming the ecological relevance of biofilm associated metals. This project provides an insight into the effect of urban contaminants on stream microorganisms and contributes to the understanding of how urbanisation affects the entire stream ecosystem. Novel tools emerging from the use of biofilm as an indicator of stream health will assist land planners, aquatic resource managers and decision makers by facilitating better assessment of urban influences on aquatic environments"--Abstract.
Download or read book Metal Effects on Freshwater Microbial Community Composition Structure and Function in an Urban Stream written by Alescia Roberto and published by . This book was released on 2018 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: In urban streams, contaminants such as antibiotics, ARGs, nutrients, and metals, co-occur with anthropogenic activities. These toxicants can have a profound effect on microbial community composition, which may, in turn, affect microbial community function. Such results suggest that the composition and function of microbial communities are consequences of their response to environmental stimuli. However, the differing physiological responses of microorganisms to environmental stressors suggests that community structure may be an important factor driving the community's functional responses to avoid or reduce the effects associated with physiochemical changes in the environment. With the projected increase in concurrent environmental stressors associated with the anthropogenic activity, there is a need to understand how microbial communities respond to compounded stressors. The overarching question for my dissertation is: how does chemical stress (in the form of heavy metals) interact with other environmental factors (including hydrologic conditions and nutrients) to impact microbial community structure and function in streams? To address this question, I utilized a combination of trait-based molecular approaches in tandem with biological and chemical field measurements and laboratory experiments. I looked at how microbial, specifically bacterial and diatom, community composition, structure, and function were affected by different stresses that are found within urban streams.
Download or read book Reviews of Environmental Contamination and Toxicology Volume 248 written by Pim de Voogt and published by Springer. This book was released on 2019-06-17 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reviews of Environmental Contamination and Toxicology attempts to provide concise, critical reviews of timely advances, philosophy and significant areas of accomplished or needed endeavor in the total field of xenobiotics, in any segment of the environment, as well as toxicological implications.
Download or read book Effect of Heavy Metals on the Biofilm Formed by Microorganisms from Impacted Aquatic Environments written by Maria Bet√¢nia Bet√¢nia Melo De Oliveira and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aquatic environment is highly complex and diverse, consisting of several types of ecosystems that are dynamic products of complex interactions between biotic and abiotic components. Changes in the physical and chemical properties of these ecosystems can significantly affect the balance of life forms present, especially in their microbiota. Among the main pollutants present in these environments are heavy metals. Several studies demonstrate the effects of these minerals on the structure and function of microbial communities, which may develop adaptation mechanisms for survival and permanence in these sites. In addition, the resistance to heavy metals may contribute to the evolution of resistance genes to the different types of antimicrobials due to the increase of the selective pressure in the environment, becoming a public health problem. One of the adaptive mechanisms present in bacteria from impacted environments that has been frequently investigated is the formation of biofilms. Recent studies have reported significant changes in the structure and amount of biofilm formed in the presence of different metals, and consequently, an increase in the tolerance to these pollutants and antimicrobials. This review will discuss the effects of some metals on bacterial biofilms and their consequences for the marine environment.
Download or read book The Seine River Basin written by Nicolas Flipo and published by Springer Nature. This book was released on 2021-01-30 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book reviews the water-agro-food and socio-eco-system of the Seine River basin (76,000 km2), and offers a historical perspective on the river’s long-term contamination. The Seine basin is inhabited by circa 17 million people and is impacted by intensive agricultural practices and industrial activities. These pressures have gradually affected its hydrological, chemical and ecological functioning, leading to a maximum chemical degradation between the 1960s and the 1990s. Over the last three decades, while major water-quality improvements have been observed, new issues (e.g. endocrine disruptors, microplastics) have also emerged. The state of the Seine River network, from the headwaters to estuary, is increasingly controlled by the balance between pressures and social responses. This socio-ecosystem provides a unique example of the functioning of a territory under heavy anthropogenic pressure during the Anthropocene era. The achievements made were possible due to the long-term PIREN Seine research program, established in 1989 and today part of the French socio-ecological research network “Zones Ateliers”, itself part of the international Long-term Socio-economic and Ecological Research Network (LTSER). Written by experts in the field, the book provides an introduction to the water budget and the territorial metabolism of the Seine basin, and studies the trajectories and impact of various pollutants in the Seine River. It offers insights into the ecological functioning, the integration of agricultural practices, the analysis of aquatic organic matter, and the evolution of fish assemblages in the Seine basin, and also presents research perspectives and approaches to improve the water quality of the Seine River. Given its scope, it will appeal to environmental managers, scientists and policymakers interested in the long-term contamination of the Seine River.
Download or read book The Micro ecology of Stream Biofilm Dynamics Environmental Drivers Successional Processes and Forensic Applications written by Jennifer M. Lang and published by . This book was released on 2015 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial activity has an essential role in ecosystem processes, and in stream ecosystems, biofilms are the base of the food web that is fueled by photosynthesis and they are integral to nutrient processing. Stream biofilms are microbial communities of algae, bacteria, fungi, and protozoa encased in an extracellular polymeric substance (EPS) (molecules secreted by the microbes) that are attached to a substrate (e.g. rocks, leaves) in an aqueous environment. The substrate categorizes the biofilm, and organic matter like leaves and carrion such as salmon carcasses are important substrates for nutrient dynamics. In special instances, human remains may be deposited into streams and colonized by biofilms; therefore, assessing these biofilms can have direct application to the forensic sciences. Stream ecologists have extensively investigated how environmental factors influence algal community composition, while environmental microbiologists have focused on the role of bacterial communities in nutrient dynamics. My dissertation marries these two approaches by considering biofilm communities as a functioning ecosystem and uses ecological theory as a framework to understand the dynamics of this micro-ecosystem. This framework uses aspects from landscape ecology within a larger context of community ecology to explain how the development of biofilm communities is altered by environmental factors. In addition, this framework was used to investigate biofilm development on carrion (dead animal) in a forensic science context.
Download or read book Issues in Environment Health and Pollution 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 2604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Issues in Environment, Health, and Pollution: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Environment, Health, and Pollution. The editors have built Issues in Environment, Health, and Pollution: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Environment, Health, and Pollution in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Environment, Health, and Pollution: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Bacterial Community Composition in Stream Biofilms is Influenced by Algal Response to Varying Light and Phosphorus Ratios written by Yu-rui Chang and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Strong correlations between bacterial communities and algal seasonal succession have been previously observed. In aquatic systems, dissolved organic carbon derived by algae is an important resource for bacteria. Light and phosphorus availability are two factors that influence biomass and abundance of algae, and the changes will be reflected in the bacterial portion of the microbial community. Glycolate is an algal-specific exudate produced under excess light conditions. Glycolate uptake by bacteria has been shown to correlate with algal primary productivity. Bacterial populations that utilize glycolate possess the gene, glycolate oxidase subunit D (glcD). This gene was used as a marker to identify changes in specific bacterial populations that respond to algal exudates. In this study, development of periphyton biofilms in an experimental stream system was monitored across different light and phosphorus levels. Samples were collected every two days for community and chemistry analyses. Bacterial communities were monitored using DNA fingerprinting techniques based on ribosomal RNA genes and the glcD gene. We demonstrated that bacterial community composition changed significantly over the course of biofilm development, and light and phosphorus availability contributed to those differences in community composition. Our results suggest that a strong coupling between carbon flow and bacterial community composition. These results increase our understanding of the ecological drivers that impact benthic biofilm communities that carry out transformation of nutrients in streams.
Download or read book Urban Discharges and Receiving Water Quality Impacts written by J. B. Ellis and published by Elsevier. This book was released on 2013-10-22 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Urban Discharges and Receiving Water Quality Impacts covers the proceedings of a seminar organized by the IAWPRC/IAHR Sub-Committee for Urban Runoff Quality Data, as part of the IAWPRC 14th Biennial Conference. The book presents papers that discuss the methods and procedures for the control and management of urban discharges. The topics covered in the text include the impact of the quality and quantity of overflow on receiving water; impact of nonpoint pollution on a great lakes freshwater harbor-estuary; and microbiological impacts of storm sewer overflows. The book also tackles hydraulic performance and control of pollutants discharged from a combined sewer storage overflow; urban stormwater reduction and quality improvement through the use of permeable pavements; and water quality indices for the management of surface water quality. The text will be of great use to researchers and professionals concerned with effects of urban discharge on aquatic environment.
Download or read book Effects of Heavy Metals on Certain Microbial Processes in Freshwater Sediments written by Anders Broberg and published by . This book was released on 1983 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt: In experiments with sediment-water systems the effects of Copper, Cadmium, Zinc and Lead on electron transport system activity (ESTA), rate of nitrate reduction (except Pb) and nitrification were studied. All experiments were performed with eutrophic surface sedoments, contaminated with salts of different metals.
Download or read book Microbial Diversity and Ecosystem Functioning in Fragmented Rivers Worldwide written by Lunhui Lu and published by Frontiers Media SA. This book was released on 2023-11-27 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dams or barriers are among the most significant anthropogenic threats to global freshwater ecosystems, although they provide invaluable services for shipping, hydropower generation, flood protection, and storage of drinking and irrigation water. River fragmentations due to dams and barriers lead the aquatic landscape into isolated river sections, resulting in hydromorphological discontinuities along longitudinal or lateral gradients. Fragmented river habitats are unstable. They experience uncertain disturbances in both time and space with random and complex hydrological and environmental processes, such as water flow, particulate matter sedimentation, reservoir regulation, and terrestrial input. The diversity, composition, functionality, and activity of microbial communities are important indicators of river ecosystem functions and services. Yet, river fragmentations are likely to disrupt and reconstruct microbial communities, redirecting the patterns of biogeochemical cycles of biogenic elements. Methodology, such as mathematical models, is still limited to describing and elucidating microbial processes under changing hydrological environments in the fragmented rivers. Thus, how do the riverine microbial communities and ecosystem functions respond to the fragmentation in rivers? This Research Topic represents a collective focus on microbial ecology, functional diversity, and new microbial modeling in fragmented rivers. We wish to present new findings in community assembly mechanisms, biotic interactions, functional diversity, and ecosystem functioning responses to the river fragmentations. New perspectives will also provide us with deep insights into the ecological effects of river fragmentation. This Research Topic aims to present the original research articles and reviews to provide new findings on microbial diversity and ecosystem functioning in fragmented rivers worldwide. We welcome original research, reviews, mini-reviews, opinions, methods, hypotheses and theories, and perspectives. The directions include but are not limited to the following aspects: - The continuum of the microbial community in responses to dams or barriers. - Novel microbial community assembly mechanisms, functional traits, and biotic interactions in fragmented rivers at local, regional, and global scales. - Functional genes, functional groups, and functional diversity in driving biogenic element cycles. - Mathematical modeling in aquatic microbial ecology.
Download or read book Biogeochemistry of Wetlands written by K. Ramesh Reddy and published by CRC Press. This book was released on 2022-09-10 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt: The globally important nature of wetland ecosystems has led to their increased protection and restoration as well as their use in engineered systems. Underpinning the beneficial functions of wetlands are a unique suite of physical, chemical, and biological processes that regulate elemental cycling in soils and the water column. This book provides an in-depth coverage of these wetland biogeochemical processes related to the cycling of macroelements including carbon, nitrogen, phosphorus, and sulfur, secondary and trace elements, and toxic organic compounds. In this synthesis, the authors combine more than 100 years of experience studying wetlands and biogeochemistry to look inside the black box of elemental transformations in wetland ecosystems. This new edition is updated throughout to include more topics and provide an integrated view of the coupled nature of biogeochemical cycles in wetland systems. The influence of the elemental cycles is discussed at a range of scales in the context of environmental change including climate, sea level rise, and water quality. Frequent examples of key methods and major case studies are also included to help the reader extend the basic theories for application in their own system. Some of the major topics discussed are: Flooded soil and sediment characteristics Aerobic-anaerobic interfaces Redox chemistry in flooded soil and sediment systems Anaerobic microbial metabolism Plant adaptations to reducing conditions Regulators of organic matter decomposition and accretion Major nutrient sources and sinks Greenhouse gas production and emission Elemental flux processes Remediation of contaminated soils and sediments Coupled C-N-P-S processes Consequences of environmental change in wetlands# The book provides the foundation for a basic understanding of key biogeochemical processes and its applications to solve real world problems. It is detailed, but also assists the reader with box inserts, artfully designed diagrams, and summary tables all supported by numerous current references. This book is an excellent resource for senior undergraduates and graduate students studying ecosystem biogeochemistry with a focus in wetlands and aquatic systems.
Download or read book Periphyton written by Yonghong Wu and published by Elsevier. This book was released on 2016-08-29 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Periphyton: Functions and Application in Environmental Remediation presents a systematic overview of a wide variety of periphyton functions and applications in environmental remediation, providing readers with an understanding of the biological/ecological features of periphyton, the methodology of their study, and their application in environmental conservation. With increases in environmental stress, anthropogenic impacts, and the global decline in biodiversity, there is a pressing need for methods to assess and improve environmental quality that are rapid, reliable, and cost-effective. Periphyton is an important component of benthic communities and plays a crucial role in the functioning of microbial food webs. Because of a number of advantages, such as a short lifecycle, relative immobility, more rapid responses to environmental stress and anthropogenic impact than any metazoa, ease of sampling, availability of taxonomic/molecular identification, and standardized methodologies for temporal/spatial comparisons, there has, in recent decades, been an increased interest in periphyton as a tool in biological conservation in aquatic ecosystems. Presents case studies that help readers implement similar ecological designs Focuses on the function of periphyton in remediating destructed ecosystems Provides readers with an understanding of periphyton in practice, especially the value of periphyton in enhancing environmental and ecosystem qualities Discusses the role of periphyton in purifying water and its effect on abiotic elements
Download or read book Freshwater Microbiology written by David C. Sigee and published by John Wiley & Sons. This book was released on 2005-09-27 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique textbook takes a broad look at the rapidly expanding field of freshwater microbiology. Concentrating on the interactions between viruses, bacteria, algae, fungi and micro-invertebrates, the book gives a wide biological appeal. Alongside conventional aspects such as phytoplankton characterisation, seasonal changes and nutrient cycles, the title focuses on the dynamic and applied aspects that are not covered within the current textbooks in the field. Complete coverage of all fresh water biota from viruses to invertebrates Unique focus on microbial interactions including coverage of biofilms, important communities on all exposed rivers and lakes. New information on molecular and microscopical techniques including a study of gene exchange between bacteria in the freshwater environment. Unique emphasis on the applied aspects of freshwater microbiology with particular emphasis on biodegradation and the causes and remediation of eutrophication and algal blooms.
Download or read book Aquatic Microbial Community Structure and Function Across a Gradient of Logging Fire and Industrial Watershed Disturbance written by Caroline Emilson and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The role of microbial communities in the recovery of aquatic ecosystems from watershed disturbance has received little attention despite their important role in energy and nutrient cycling. This study investigates the structure and function of microbial communities on a standardized substrate (alder leaves) in small streams across a wide gradient of watershed disturbances. Microbial communities exhibited variation with disturbance regime with lower hydrolase enzyme activities at all disturbed streams compared to undisturbed streams, and the lowest rates of microbial decomposition, fungal biomass, and differences in microbial community composition at the most severely disturbed streams. Forest and wetland cover were identified as important watershed features that provide DOC to fuel microbial activity in aquatic ecosystems. Increasing road density within the watershed was identified as having a negative impact or association on microbial activity that appeared to be linked to inputs of inorganic solutes that were measured through increased levels of specific conductance in stream water samples. This study is one of the first of its kind and it provides some important evidence that leaf litter associated microbial communities can be influenced by factors linked to watershed disturbance and as such may be useful as indicators of watershed disturbance and potentially the state of recovery of aquatic ecosystems.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2036 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Understanding the Impacts of Organic Matter on Microbial Biofilms in Engineered Drinking Water Systems written by Lei Li and published by . This book was released on 2020 with total page 185 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increasing occurrence and severity of cyanobacterial harmful algal blooms (HABs) in freshwater have continuously challenged the safe drinking water supply. During HAB, public attention mainly focuses on the cyanotoxins, which associated with health issues, while HAB also generated massive amounts of algal cells, increasing the loading of algal organic matter (AOM) in the drinking water treatment plants (DWTPs). AOM is an algae-derived autochthonous natural organic matter (NOM), which contains high fraction of hydrophilic and nitrogenous compounds. Conventional treatment processes, comprised of coagulation, sedimentation, and granular media filtration, are known to be ineffective in completely removing NOM, including AOM [1, 2]. Although ozone has been widely adopted by water utilities to break down complex organic compounds and reduce DBP formation, ozonation practices can adversely increase concentrations of assimilable organic carbon (AOC), which in turn can be rapidly utilized and support biofilm growth in downstream filters and drinking water distribution systems [3, 4]. Currently, remain largely unknown for the growth of biofilms under the impacts of different NOM, including AOM in the filters and drinking water distribution systems (DWDSs). Therefore, the main research goal of this study is to investigate the impacts of organic matter on microbial biofilms in engineered drinking water systems (EDWSs). Specifically, the first objective of this study aimed to examine how the assembly processes and their associated factors (e.g., influent characteristics, biological interactions) drive the temporal dynamics of bacterial communities in full-scale BAC filters, which underwent ozone implementation to better handle the adverse effects of HABs. The obtained results revealed that along with the increase of bacterial taxonomic richness and evenness, stochastic processes became more crucial to determine the bacterial community assembly in the summer and autumn after ozone implementation. Moreover, their corresponding networks possessed simple network structures with lower modularity than other seasons, which implied lesser biological interactions among bacterial populations. Among the monitored physiochemical properties of filter influents, temperature and nutrient bioavailability (i.e., AOC concentrations) as well as biological interactions can be crucial drivers that impact the balance between these two processes and the taxonomic diversity of bacterial communities in BAC filters. The second objective of this study was to examine the effects of two widely present NOM, treated AOM and humic substances (HS), on biofilm development under unchlorinated DWDS conditions. Although great efforts have been made to remove NOM in DWTPs, remaining NOM still exists in the filter effluent and subsequently enter DWDSs. This unremoved NOM can support the growth of microbial biofilms in DWDS. Thus, the impact of AOM and HS on the formation, chemical composition, and microbial community structures of biofilms was evaluated. The 16S rRNA gene sequencing analyses revealed that the bacterial communities in biofilms were clustered with the organic matter types in bulk water, where Family Comamonadaceae was the most dominant but showed different temporal dynamics depending on the organic matter characteristics in bulk water. Higher diversity was observed in the biofilms grown in AOM-impacted bulk water (BFAOM) than biofilms grown in HS-impacted (BFHS) and R2A-impacted bulk water (BFR2A) as the biofilms matured. In addition, some taxa (e.g., Rhodobacteraceae, and Sphingomonadaceae) were enriched in BFAOM compared to BFHS and BFR2A. The biofilm image analysis results indicated that compared to BFHS, BFAOM and BFR2A had relatively thinner and heterogeneous physical structures with lower amounts of cell biomass, extracellular polymeric substances (EPS), and higher EPS protein/polysaccharide ratios. The third objective of this study was to elucidate how different types of organic matter, including AOM and HS, affect biomolecular compositions of biofilms and subsequent DBP formation. In order to control biofilm formation in DWDS, water utilities apply disinfectants such as chlorine or monochloramines. However, these applied disinfectants can lead to the formation of toxic DBPs due to the presence of organic-rich substances within biofilms. Therefore, the impact of organic matter composition on biomulecular composition of biofilms and their correlations with DBP formation were explored. The obtained results indicated that all biofilm samples comprised mostly of protein-like components (~90%), and to a lesser extent, humic-like components (~10%). Strong correlations were generally found between tryptophan-like substances and the studied DBP formation (R2min ≥ 0.76, P
