Download or read book Ultraviolet Resonance Raman Spectroscopy of Proteinsandprotein Components written by Robert Allen Copeland and published by . This book was released on 1986 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopy of Proteins and Protein Components written by Robert Allen Copeland and published by . This book was released on 1986 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Structure written by Robert Austin and published by Springer. This book was released on 2014-11-14 with total page 515 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is concerned with the physical aspects of molecular and electronic tunneling in biological systems, and the extent to which protein structure controls these events. The scope is very broad and this volume could almost be a textbook in biophysics. Both fundamental processes and the extrapolation to physiological events are stressed. The discussion sections are remarkably frank and offer insight into the basic problems confronting physists and chemists as they seek to apply their techniques to biological systems. This book on the physics of biomolecules reflects recent progress in understanding the biological function of the key protein molecules from detailed knowledge of their physics. New and exciting are the glasslike aspects of protein structures and the discussion of proteins as fractals. Other topics dealt with are low-temperature kinetics and reactivity, structure and charge exchange, and charge separation in photosynthetic reaction centers.

Download or read book Laser Applications in Life Sciences written by Sergeĭ Aleksandrovich Akhmanov and published by . This book was released on 1991 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Far Ultraviolet Resonance Raman Studies of Protein Components written by Leland Carter Mayne and published by . This book was released on 1988 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Deep Ultraviolet Resonance Raman Spectroscopy of Membrane Proteins written by Christopher Halsey and published by . This book was released on 2012 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopy of Nucleotides written by Daniel Charles Blazej and published by . This book was released on 1978 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopy of the Nucleicacids written by Stephen Philip Alan Fodor and published by . This book was released on 1985 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopy of the Nucleic Acids written by Stephen Philip Alan Fodor and published by . This book was released on 1985 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopy of the Tertiary Peptide Bond written by Gregory Paul Harhay and published by . This book was released on 1992 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescence and UV Resonance Raman Spectroscopy Studies of HU Protein Binding to DNA written by Kristi Wojtuszewski Poulin and published by . This book was released on 2002 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book American Doctoral Dissertations written by and published by . This book was released on 1985 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopy of the Nucleic Acids and Their Metal Ion Complexes written by Joseph Raymond Perno and published by . This book was released on 1987 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman and Fluorescence Studies of Folded and Unfolded Conformations of the Membrane Protein OmpA written by Katheryn Marie Sanchez and published by . This book was released on 2010 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation focuses on the folding dynamics of a bacterial membrane protein, Outer Membrane Protein A (OmpA), using fluorescence and Ultraviolet resonance Raman spectroscopy. Our model [Beta]-barrel membrane protein, OmpA, contains five native anchoring Tryptophan residues. The spectroscopic properties of trp residues are highly sensitive to the local environment, making it an ideal probe for membrane protein folding studies. Utilizing trp fluorescence, refolding studies were performed on single trp mutants of OmpA to determine the thermodynamic stability of these trp mutants. The important noncovalent interactions that promote stability in OmpA are pairwise aromatic interactions and hydrogen bonds with the N1H moiety of trp. Refolding studies were also performed on truncated single-trp mutants, in which the soluble domain of the protein was removed. These studies resulted in increased stability relative to the full-length protein and suggest the absence of the soluble domain may destabilize the unfolded transmembrane domain. Ultraviolet resonance Raman spectroscopy (UVRR) is a powerful vibrational technique that can selectively probe different biological chromophores depending on excitation wavelength. Excitation wavelength dependence studies were performed on OmpA using wavelengths from 206.5 nm - 236.5 nm. This study determined an optimal excitation wavelength of 228-nm to selectively enhance signal from trp residues in OmpA. Additionally, UVRR was used to monitor changes in trp environmental hydrophobicity, hydrogen bonding, and dihedral torsion angle in different conformations of OmpA. The first UVRR spectra were collected of OmpA in a highly scattering environment and show differences in folded and unfolded conformations of the protein, showing the applicability of this technique to study membrane protein folding. UVRR spectra were collected of trp mutants of OmpA at different time points in the folding/insertion process to determine the types of noncovalent interactions with trp residues, and the folding timescales these interactions occur. Our results indicate noncovalent interactions start to form within the first 20 minutes after initiation of folding into DMPC vesicles and continue to show subtle changes over the course of the folding process. Additionally, there is evidence for interactions between trp residues and lipids, inter-residue hydrogen bonding, and amino-aromatic interactions.

Download or read book Applications of UV Resonance Raman and Micro Raman Spectroscopy for the Rapid Characterization of Bacteria and the Study of Polymers written by Suneet Chadha and published by . This book was released on 1993 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultraviolet Resonance Raman Spectroscopic Studies of Nucleic Acids and Proteins written by Christine Anne Grygon and published by . This book was released on 1989 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Monitoring Membrane Protein Structural Changes and Interactions Via Deep UV Resonance Raman Spectroscopy written by Mia C. Brown and published by . This book was released on 2015 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins perform a variety of functions within our cells. They transport nutrients and waste across the lipid barrier, transmit signals from one part of the body to another, and run our immune system. However, despite their ubiquitous and vital presence in all organisms, relatively little is known about this class of proteins compared to their soluble counterparts. In this work I have set out to use deep UV resonance Raman (DUVRR) spectroscopy to characterize structural and environmental transitions of proteins and applied the results to studies involving intramembrane proteases and their substrates. DUVRR has been used extensively to observe protein secondary structure. This work contains the results of three main studies I have conducted during my graduate career. In the first I show results from the first experiment to look simultaneously at both secondary and tertiary structure as a protein transitions from a molten globule to ordered state. In the second study tracks the structural and environmental changes of a small peptide as it transitions from a soluble, disordered state through two membrane-bound, structurally ordered states. I was then able to apply the findings of these experiments to study intramembrane proteolysis, wherein I describe substrate characteristics and their interactions with proteases.