Download or read book Two photon Dual Channel Fluctuation Correlation Spectroscopy written by John Safwat Eid and published by . This book was released on 2002 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Two photon Fluctuation Correlation Spectroscopy written by Keith Michael Berland and published by . This book was released on 1995 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented. There are two biophysical applications proposed and discussed in this thesis. The first is the study of particle mobilities and intracellular diffusion. Measurements of particle diffusion in the cytoplasm of live cells are presented. The second application is the detection of protein aggregation in solution using two-photon scanning FCS. Measurements are shown demonstrating the potential to study aggregation equilibria at low protein concentrations, and the dissociation reactions of several oligomeric proteins are studied. The possibility to measure dissociation kinetics is also demonstrated.
Download or read book Fluorescence Correlation Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.
Download or read book Reviews in Fluorescence 2006 written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2007-02-05 with total page 621 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third volume in the Reviews in Fluorescence series. To date, two volumes have been both published and well received by the scientific community. Several book reviews have also favorably described the series as an "excellent compilation of material which is well balanced from authors in both the US and Europe". Of particular mention we note the recent book review in JACS by Gary Baker, Los Alamos. In this 3rd volume we continue the tradition of publishing leading edge and timely articles from authors around the world. We hope you find this volume as useful as past volumes, which promises to be just as diverse with regard to content. Finally, in closing, we would like to thank Dr Kadir Asian for the typesetting of the entire volume and our counterparts at Springer, New York, for its timely publication. Professor Chris D. Geddes Professor Joseph R. Lakowicz August 20*^ 2005.
Download or read book Photon Correlation Spectroscopy and Velocimetry written by H. Cummins and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 601 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following the first Capri School on Photon Correlation Spectroscopy held in July 1973 and published earlier in this series (Series B: Physics v.3) a second Capri NATO Advanced Study Institute on this topic was held at the Hotei Luna from 26 July to 6 August 1976. This volume contains the invited lecture courses and seminars and some of the contributed seminars presented at this Institute. Much had happened in the field in the intervening three years and it was the intention of the Organising Committee to build on the previous courses • without detailed repetition of fundamentals. and to extend the coverage widely over the use of photon-correla tion methods for the temporal or spectral analysis of fluctuating light sources. In particular, the rapid expansion of these methods for the measurement of macroscopic motion by Laser Doppler Veloci metry was given special emphasis as is indicated in the title. The members of the Organizing Committee were: E R Pike, RSRE, Malvern, UK } _ Co-directors H Z Cummins, CCNY, New York, USA M Bertolotti, University of Rome, Italy - Local Organiser P Pusey, RSRE, Malvern, UK - Treasurer V DeGiorgio, CISE, Milan, Italy P Lallemand, ENS, Paris, France Pierre de Gennes assisted the Committee during the planning of the Institute but was unfortunately prevented at the last minute from attending.
Download or read book Fluorescence Fluctuation Spectroscopy FFS Part A written by and published by Academic Press. This book was released on 2012-12-28 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.
Download or read book Fluorescence Fluctuation Spectroscopy FFS Part B written by and published by Academic Press. This book was released on 2012-12-29 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells
Download or read book Advances In Multi photon Processes And Spectroscopy Vol 2 written by Shih-i Chu and published by World Scientific. This book was released on 1986-05-01 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the recent rapid growth in both experimental and theoretical studies of multiphoton processes and multiphoton spectroscopy of atoms, ions and molecules in chemistry, physics, biology, material sciences, etc. It contains papers readable by active researchers and by those who intend to enter it. Theory and experiment are equally emphasized, and each review article is written in a self-contained manner by experts in the field so that readers learn the subject without much preparation.
Download or read book Photon Correlation and Light Beating Spectroscopy written by H. Cummins and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 575 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the invited lectures and seminars and abstracts of the contributed seminars presented at the NATO Advanced Study Institute on Photon Correlation and Light Beating Spectroscopy held at the Centro Caprense Di Vita E Di Studi Ignazio Cerio, Capri, Italy, July 16-27, 1973. The Institute was organized to provide a com~rehen8ive presentation of this new and rapidly developing field for those interested in applying these techniques to problems in many areas including Physics, Biology, Engineering and Chemistry. The lectures were divided into three principal categories: the first Basic Theory (Photon Statistics and Correlation, Scattering Theory), secondly Instrumentation (Correlation Techniques, Light Beating), and the third Areas of Application (Gas and Liquid Dynamics, Critical Phenomena, Biology). The seminars provided detailed presentations of applications to a number of specific problems. - Although the selection of topics was inevitably li mi ted, i t was the hope of the organizing committee that the lectures would provide a broad coverage appropriate for the needs of the interdisciplinary audience represented by the participants, and that this volume would serve for some years to come as a useful introduction for those entering the field. The members of the Organizing Committee were: E.R. Pike, RRE, Malvern U.K. } Co-directors H.Z. Cummins, New York University M. Bertolotti, Universita di Roma - LocalOrganizer J.M. Vaughan, RRE, Malvern, U.K. Secretary H. Swinney, New York University Treasurer P. Lallemand, Ecole Normale Superieure, Paris H. Haken, Universitat Stuttgart, Germany.
Download or read book Comprehensive Biophysics written by and published by Academic Press. This book was released on 2012-04-12 with total page 3533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource
Download or read book Two Applications of Photon Correlation Spectroscopy written by James Clyde Selser and published by . This book was released on 1975 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biophotonics written by Xun Shen and published by Springer Science & Business Media. This book was released on 2006-01-04 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now well established that all living systems emit a weak but permanent photon flux in the visible and ultraviolet range. This biophoton emission is correlated with many, if not all, biological and physiological functions. There are indications of a hitherto-overlooked information channel within the living system. Biophotons may trigger chemical reactivity in cells, growth control, differentiation and intercellular communication, i.e. biological rhythms. The basic experimental and theoretical framework as well as the technical problems and the wide field of applications in the biotechnical, biomedical engineering, engineering, medicine, pharmacology, environmental science and basic science fields are presented in this book. To promote the dialog and mutual penetration between biophoton research and photon technology is one of the important goals for the International Conference on Biophotons & Biophotonics 2003, and is developed and presented in Biophotonics: Optical Science and Engineering in the 21st Century.
Download or read book Photons and Quantum Fluctuations written by E. R. Pike and published by CRC Press. This book was released on 2020-08-26 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: Based on a special ONR seminar, Photons and Quantum Fluctuations draws together discoveries in nonclassical or "silent" light for research workers and postgraduates in quantum optics. With nonclassical light, noise is reduced in amplitude below that expected by previous applications of the uncertainty principles. Historians of science who wish to ponder the philosophical implications of these developments may also find this a useful volume.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2007 with total page 924 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Download or read book Advanced Photon Counting written by Peter Kapusta and published by Springer. This book was released on 2015-04-23 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Download or read book Introduction to Polymers written by Robert J. Young and published by CRC Press. This book was released on 2011-06-27 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thoroughly updated, Introduction to Polymers, Third Edition presents the science underpinning the synthesis, characterization and properties of polymers. The material has been completely reorganized and expanded to include important new topics and provide a coherent platform for teaching and learning the fundamental aspects of contemporary polymer
