Download or read book Two Bacteripohage T4 Gene Products which Regulate Gene Expression Affect DNA Structure written by Karl M. Sirotkin and published by . This book was released on 1978 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Gol Site written by Wendy Cooley Champness and published by . This book was released on 1982 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Expression of Two Early Bacteriophage T4 Genes and the Role of One of the Gene Products in a Multi enzyme Complex written by Christina Thylén and published by . This book was released on 1990 with total page 58 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Synthesis and the Expression of Two Classes of Late Genes of Bacteriophage T4 written by Robert David Bruner and published by . This book was released on 1969 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacteriophage T4 written by Christopher K. Mathews and published by . This book was released on 1983 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Organization of T4 Bacteriophage Genes and Gene Products Involved in DNA Precursor Biosynthesis written by Yeong Wang and published by . This book was released on 1989 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is necessary. Therefore, cloning and overexpression of T4 gene 42 are needed for these studies. An 1.8-kb Eco RI restriction fragment of a T4 multiple mutant BK536, containing an amber mutation in gene 42, has been cloned into pUC19 plasmid vector in Escherichia. coil. Genetic and biochemical examination of the cloned T4 DNA fragment has revealed that it contains the entire gene 42 coding sequence. The cloned amber mutant gene was converted to a wild type gene by site-directed mutagenesis, and the wild type gene 42 was overexpressed with the pT7 expression vector system. dCMP hydroxymethylase expressed from the cloned gene 42 was purified to homogeneity. The specific activity and N-terminal amino acid sequence of the purified enzyme were determined. The specific activity of the purified cloned gene product closely agreed with the value of the enzyme purified- from phage-infected E. colt cells. Nterminal amino acid determination has confirmed the open reading frame of gene 42, deduced from nucleotide sequence of gene 42. Purified dCMP hydroxymethylase has also been used to study its crystal structure and catalytic mechanism by collaboration with other research groups. In addition, purified dCMP hydroxymethylase was immobilized on Affi-Gel to prepare an affinity column, which was used to study protein-protein interaction among dNTP biosynthetic enzymes and with other DNA metabolic proteins. The proteins which specifically interact with dCMP hydroxymethylase were identified by SDS polyacrylamide gel electrophoresis followed by fluorography, Western blotting, and two dimensional gel electrophoresis. It was earlier found that T4-encoded thymidylate synthase and dihydrofolate reductase function not only in deoxyribonucleotide biosynthesis but are also structural components of the phage baseplate. Two deletion mutants containing deletion in the td gene, encoding thymidylate synthase, and the frd gene, encoding dihydrofolate reductase, were carefully characterized, and used to reevaluate structural role of these two enzymes.
Download or read book Molecular Biology of Bacteriophage T4 written by Jim D. Karam and published by ASM Press. This book was released on 1994 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new text highlights the value of this biological system as a research and teaching tool. The book is a sequel to the 1983 edition and is organized into 6 major sections: DNA metabolism, regulation of gene expression, morphogenesis, structure of selected proteins, host–phage interactions, and laboratory experiments in T4 molecular genetics. Since T4 has played a central role in the development of molecular biology as an academic discipline, the themes presented in this book provide a framework for designing graduate and undergraduate courses in prokaryotic genetics and biochemistry.
Download or read book Bacteriophages written by David R. Harper and published by Springer Nature. This book was released on 2021-01-30 with total page 1376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first major reference work dedicated to the mannifold industrial and medical applications of bacteriophages provides both theoretical and practical insights into the emerging field of bacteriophage biotechnology. The book introduces to bacteriophage biology, ecology and history and reviews the latest technologies and tools in bacteriophage detection, strain optimization and nanotechnology. Usage of bacteriophages in food safety, agriculture, and different therapeutic areas is discussed in detail. This book serves as essential guide for researchers in applied microbiology, biotechnology and medicine coming from both academia and industry.
Download or read book Recoding Expansion of Decoding Rules Enriches Gene Expression written by John F. Atkins and published by Springer Science & Business Media. This book was released on 2010-03-10 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: The literature on recoding is scattered, so this superb book ?lls a need by prov- ing up-to-date, comprehensive, authoritative reviews of the many kinds of recoding phenomena. Between 1961 and 1966 my colleagues and I deciphered the genetic code in Escherichia coli and showed that the genetic code is the same in E. coli, Xenopus laevis, and guinea pig tissues. These results showed that the code has been c- served during evolution and strongly suggested that the code appeared very early during biological evolution, that all forms of life on earth descended from a c- mon ancestor, and thus that all forms of life on this planet are related to one another. The problem of biological time was solved by encoding information in DNA and retrieving the information for each new generation, for it is easier to make a new organism than it is to repair an aging, malfunctioning one. Subsequently, small modi?cations of the standard genetic code were found in certain organisms and in mitochondria. Mitochondrial DNA only encodes about 10–13 proteins, so some modi?cations of the genetic code are tolerated that pr- ably would be lethal if applied to the thousands of kinds of proteins encoded by genomic DNA.
Download or read book Phage and the Origins of Molecular Biology written by John Cairns and published by CSHL Press. This book was released on 2007 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1966 as a 60th birthday tribute to Max Delbrck, this influential work is republished as "The Centennial Edition." The book was hailed as "[introducing] into the literature of science, for the first time, a self-conscious historical element in which the participants in scientific discovery engage in writing their own chronicle ("Journal of History of Biology").
Download or read book Bacterial Chromatin written by Remus T. Dame and published by Springer Nature. This book was released on with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Prokaryotic Metabolism and Physiology written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2019-05-16 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive and up-to-date review of key metabolic processes in bacteria and archaea and how metabolism is regulated under various conditions.
Download or read book Gene Cloning and Manipulation written by Christopher Howe and published by Cambridge University Press. This book was released on 2007-07-12 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Download or read book Serpin Structure and Evolution written by James Whisstock and published by Academic Press. This book was released on 2011-11-16 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems. This volume in the Methods in Enzymology series comprehensively covers the topic of serpins With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems
Download or read book Studies on the Biological Role and Genetics of the T4 Bacteriophage RNA Ligase written by Judith Marie Runnels and published by . This book was released on 1981 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Molecular Biology of Plant Cells written by H. Smith and published by Univ of California Press. This book was released on 1977-01-01 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
