Download or read book Trapping Single Molecules with a Solid State Nanopore written by Marc Herman Gershow and published by . This book was released on 2008 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: I describe a system for measuring and manipulating single molecules using a solid state nanopore, a small hole in an insulating film fashioned using solid-state Silicon processing techniques. In this work, pores less than 10 nanometers in diameter in 20-30 nm thick Silicon Nitride membranes are used to join two reservoirs of salt water. DNA molecules added to one reservoir are forced through the nanopore by an applied voltage bias. Single molecules of DNA are detected passing through the pore by a blockage of the ionic current between the reservoirs. The depth and duration of the current blockages reveal information about the molecule's geometry while the shape of the blockage depends on the molecule's conformation. If the driving voltage is reversed soon after a molecule has passed through the nanopore, the same molecule can be "recaptured," induced to pass through the pore a second time. This process reveals information about the dynamics of the molecule outside of and its interaction with the nanopore. The recapture process can be repeated multiple times to form a single molecule trap.
Download or read book Single Molecule Sensing Beyond Fluorescence written by Warwick Bowen and published by Springer Nature. This book was released on 2022-03-01 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an interesting snapshot of recent advances in the field of single molecule nanosensing. The ability to sense single molecules, and to precisely monitor and control their motion is crucial to build a microscopic understanding of key processes in nature, from protein folding to chemical reactions. Recently a range of new techniques have been developed that allow single molecule sensing and control without the use of fluorescent labels. This volume provides an overview of recent advances that take advantage of micro- and nanoscale sensing technologies and provide the prospect for rapid future progress. The book endeavors to provide basic introductions to key techniques, recent research highlights, and an outlook on big challenges in the field and where it will go in future. It is a valuable contribution to the field of single molecule nanosensing and it will be of great interest to graduates and researchers working in this topic.
Download or read book Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter and Force Based Methods written by and published by Academic Press. This book was released on 2010-07-09 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Download or read book Engineered Nanopores for Bioanalytical Applications written by Joshua B. Edel and published by William Andrew. This book was released on 2013-03-19 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding.This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. - Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials - Introduces the techniques of manufacturing solid state materials with functional nanopores - Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Download or read book Molecular and Nano Tubes written by Oliver Hayden and published by Springer Science & Business Media. This book was released on 2011-08-04 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular- and Nano-Tubes summarizes recent advancements in the synthesis, fabrication and applications of tubular structures. An interdisciplinary overview of innovative science focused on tubular structures is provided. The reader is offered an overview of the different fields that molecular and nano tubes appear in, in order to learn the fundamental basics as well as the applications of these materials. This book also: Shows how nanotechnology creates novel materials by crossing the barriers between biology and material science, electronics and optics, medicine and more Demonstrates that tubes are a fundamental element in nature and used in disparate applications such as ion channels and carbon nanotubes Molecular- and Nano-Tubes is an ideal volume for researchers and engineers working in materials science and nanotechnology.
Download or read book Polymer Translocation written by M. Muthukumar and published by CRC Press. This book was released on 2016-04-19 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymer translocation occurs in many biological and biotechnological phenomena where electrically charged polymer molecules move through narrow spaces in crowded environments. Unraveling the rich phenomenology of polymer translocation requires a grasp of modern concepts of polymer physics and polyelectrolyte behavior. Polymer Translocation discusse
Download or read book Handbook of Single Molecule Biophysics written by Peter Hinterdorfer and published by Springer Science & Business Media. This book was released on 2009-12-24 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Download or read book Confining Electrochemistry to Nanopores written by Yi-Tao Long and published by Royal Society of Chemistry. This book was released on 2020-10-26 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: The nanopore provides a highly electrochemically confined space within which single-molecule characteristics can be efficiently converted into measurable electrochemical signatures with high temporal and current resolution. Aimed at developing the concept of the electrochemical confined space in analysing single molecules, this book serves as a stepping-stone to many exciting discoveries in nanopore-based analysis of biological processes and chemical reactions in confined space. The field of nanopore sensors is growing rapidly, but there have been no new books on nanopore technology that provide an overview of the research on nanopore-based sensing until now. The book provides a good source of nanopore studies for researchers interested in and working in the general areas of electrochemistry and nanobiotechnology, especially on nanopore sensors.
Download or read book Solid state Nanopore Sensors for Nucleic Acid Analysis written by Bala Murali K. Venkatesan and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanopore DNA analysis is an emerging technique that involves electrophoretically driving DNA molecules through a nano-scale pore in solution and monitoring the corresponding change in ionic pore current. This versatile approach permits the label-free, amplification-free analysis of charged polymers (single stranded DNA, double stranded DNA and RNA) ranging in length from single nucleotides to kilobase long genomic DNA fragments with subnanometer resolution. Recent advances in nanopores suggest that this low-cost, highly scalable technology could lend itself to the development of third generation DNA sequencing technologies, promising rapid and reliable sequencing of the human diploid genome for under $1000. Here, we report the development of versatile, nano-manufactured Al2O3 solid-state nanopores and nanopore arrays for rapid, label-free, single-molecule detection and analysis of DNA and protein. This nano-scale technology has proven to be reliable, affordable, and mass producible, and allows for integration with VLSI processes. A detailed characterization of nanopore performance in terms of electrical noise, mechanical robustness and materials analysis is provided, and the functionality of this technology in experimental DNA biophysics is explored. A framework for the application of this technology to medical diagnostics and sequencing is also presented. Specifically, studies involved the detection of DNA-protein complexes, a viable strategy in screening methylation patterns in panels of genes for early cancer detection, and the creation of lipid bilayer coated nanopore sensors, useful in creating hybrid biological/solid-state nanopores for DNA sequencing applications. The concept of a gated nanopore is also presented with preliminary results. The fabrication of this novel system has been enabled by the recent discovery of graphene, a highly versatile material with remarkable electrical and mechanical properties. Direct modulation of the nanopore conductance was observed through the application of potentials to the graphene gate. These exciting results suggest this technology could potentially be useful in slowing down or trapping a DNA molecule in the pore, thereby enabling solid-state nanopore sequencing.
Download or read book Using Synthetic Nanopores for Single Molecule Analyses Detecting SNPs Trapping DNA Molecules and the Prospects for Sequencing DNA written by and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Molecule Enzymology Nanomechanical Manipulation and Hybrid Methods written by and published by Academic Press. This book was released on 2017-01-03 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion
Download or read book Solid State Nanopores written by Jean-Pierre Leburton and published by Springer Nature. This book was released on 2023-09-30 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume provides an overview of the recent advances in solid-state nanopore technology, featuring contributions by leading experts in the field. It discusses several aspects of solid-state nanopores, covering their fabrication as well as multiple biosensing applications. It successfully bridges the gap between various scientific and engineering disciplines and highlights the progress made in this area. This title is a useful tool for acquiring basic knowledge of this field and following recent progress. It is a valuable contribution to the area of nanopore biosensing and is of interest to graduate students, postdocs, or senior researchers working in the fields of physical chemistry, biochemistry, bio- and electrical engineering, and biophysics.
Download or read book New Approach in Fabrication of Solid State Nanopore for Bio Sensing Applications written by Wing Hei Harold Kwok and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Molecule Delivery and Detection Into Crowded Environment with a Solid state Nanopore written by Chi Cheng Chau and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanopores for Bioanalytical Applications written by Joshua Edel and published by Royal Society of Chemistry. This book was released on 2012-09-05 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioanalytical applications in nanopores are an exciting field of research which have seen rapid development over the last ten plus years. This book focuses uniquely on this research area providing an insight from the world leading experts in the area. Based on the proceedings of the 1st International Conference on Nanopores for Bioanalytical Applications (Lanzarote, 2012), it will provide a snapshot of this exciting field for the nanopores community. It describes the latest experimental and fabrication protocols necessary to carry out nanopore-based experiments and covers a wide variety of topics. These include: biological pores, solid state pores, hybrid structures, fundamental aspects, work on the underlying (bio)physics and applications in biosensing and DNA sequencing. The multidisciplinary approach to this topic makes the book suitable for readers in both academia and industry.
Download or read book DNA Barcoding written by Robert DeSalle and published by Springer Nature. This book was released on with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nanotechnology Characterization Tools for Biosensing and Medical Diagnosis written by Challa S.S.R. Kumar and published by Springer. This book was released on 2018-05-02 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eighth volume of a 40 volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about Nanotechnology Characterization Tools for Biosensing and Medical Diagnosis. Modern applications and state-of-the-art techniques are covered and make this volume an essential reading for research scientists in academia and industry.
