Download or read book Transcriptional Regulation During the G1 to S Transition of the Yeast Cell Cycle written by Yan Zhu and published by . This book was released on 1996 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation During the GI to S Transition of the Yeast Cell Cycle written by Yan Zhu and published by . This book was released on 1996 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation of the G1 S Cell Cycle Transition in Saccharomyces Cerevisiae written by Herman Wijnen and published by . This book was released on 2000 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of G1 Phase Progression written by Johannes Boonstra and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this contribution, several specialists describe the current knowledge on the molecular networks that regulate cell cycle progression, with an emphasis on the G1 phase of the cell cycle. The first part of Regulation of G1 Phase Progression is concerned with the individual molecules that form the network, including cyclins, cyclin-dependent kinases, inhibitors of these kinases and retinoblastoma and p53. The second section describes the signaling cascades by which external factors influence the cell cycle network, including mitogens, the extracellular matrix, nutrients and oxygen radicals. The last section describes the effects of specific external conditions on cell cycle progression and are presented such as serum starvation and subsequent re-addition and stress conditions (heat, osmolarity). The final two chapters describe the relation between cell cycle progression with cell differentiation and with apoptosis.
Download or read book Spatio Temporal Regulation of Cell Cycle Transcription written by Umut Eser and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The Start checkpoint regulates cell cycle commitment and associated transcription in the budding yeast, Saccharomyces cerevisiae. It was previously shown that commitment to cell division corresponds to activating the positive feedback loop of G1 cyclins controlled by the transcription factors SBF and MBF. Around this pivotal cell cycle event, over 300 genes (G1/S regulon) are expressed to facilitate the G1/S transition. Despite its importance, little was known about distinct temporal regulation within the G1/S regulon. We found that SBF and MBF target genes have a well-defined distribution of transcriptional activation times. We also showed that activation of G1 cyclins precedes the activation of the bulk of the G1/S regulon, which we named 'feedback-first' regulation. In budding yeast, feedback-first regulation ensures that commitment to cell division occurs before large-scale changes in transcription. Thus, the transition can be viewed as a two-step process whereby the decision to divide precedes synthesis of the cellular machinery required for division. Furthermore, we found that feedback-first regulation is conserved in the related yeast S. bayanus as well as human cells. This finding highlighted the importance of understanding the molecular mechanisms through which co-regulated genes can have distinct activation dynamics. We showed that timing is partially explained by the combinatorial use of SBF and MBF transcription factors, which implement a logical OR function for gene activation. In addition to combinatorial use of transcription factors, we analyzed genome-wide chromosome conformation capture data to examine the potential link between the timing of gene expression and 3-D genome architecture. The early-activated genes of the G1/S regulon are significantly enriched for the number of physical contacts to the rest of the genome. Further analysis revealed two main clusters, whose interactions co-vary and whose activation time distributions are distinct. Taken together, these our work explains a significant amount of timing variation within cell cycle-dependent gene expression. Thus, we concluded that the cell utilizes both genome architecture and the combinatorial use of transcription factors to implement feedback-first regulation ensuring that commitment to cell division precedes genome-wide cell cycle-dependent transcription.
Download or read book Characterizing Cell cycle as a Global Regulator of Stochastic Transcription and Noisy Gene Expression in S Cerevisiae written by Katie Julia Quinn and published by . This book was released on 2014 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: Even in the same environment, genetically identical cells can exhibit remarkable variability, or noise, in gene expression. This expression noise impacts the function of gene regulatory networks, depending on its origins. Hence, a prerequisite for understanding or designing gene regulatory networks is characterizing the origins and statistics of the noise. Variability has been largely attributed to the inherently stochastic nature of transcription. Expression statistics from multiple organisms are consistent with an influential model of "bursty" expression, where promoters are generally inactive but infrequently produce multiple mRNA. But fluctuations in the cell environment can also contribute, leaving the origins of noise unclear. We sought to determine the origins of noise in gene expression from the synthetic tetO promoter in S cerevisiae. We use single-molecule mRNA FISH to quantify nuclear and cytoplasmic mRNA in a population expression distribution, and models of stochastic mRNA production and degradation to infer underlying transcriptional dynamics. Rather than transcriptional bursting, we find that noise is driven by large differences in transcriptional activity between the G1 and S/G2/M stage of the cell cycle. Furthermore, we quantitatively characterize these dynamics of transcription by measuring expression in cells arrested at the G1/S and G2/M transition. Promoters activate in S/G2 with probability determined by activator level. mRNA statistics from an active promoter with a single operator are Poisson; expression with multiple operators is more variable. Promoters appear to inactivate at the M/G1 transition, with lower activator levels leading to increased probability of inactivation. Thus below a certain activator threshold, all cells are inactive in G1. mRNA processing and export introduces further variability. Similar analysis of the native, chromatin-regulated PHO5 promoter yields the same results. Hence cell-cycle driven transcription dynamics may be prevalent among regulated yeast genes. The timing of S/G2 activation suggests DNA replication and chromatin maturation may be linked to repressed transcription. Cell-cycle-linked fluctuations in expression are likely to affect gene behavior in regulatory networks. This thesis advocates the importance of cellular context in gene regulation and reveals a novel role of cell-cycle as a driver of eukaryotic transcription, advancing our understanding of stochastic transcription and noise in gene expression.
Download or read book The Plant Cell Cycle written by Dirk Inzé and published by Springer Science & Business Media. This book was released on 2011-06-27 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the study of the plant cell cycle has become of major interest, not only to scientists working on cell division sensu strictu , but also to scientists dealing with plant hormones, development and environmental effects on growth. The book The Plant Cell Cycle is a very timely contribution to this exploding field. Outstanding contributors reviewed, not only knowledge on the most important classes of cell cycle regulators, but also summarized the various processes in which cell cycle control plays a pivotal role. The central role of the cell cycle makes this book an absolute must for plant molecular biologists.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book G1 S Transcriptional Regulation in Saccharomyces Cerevisiae Integrates Cell Cycle Progression and Genome Stability written by Michael Ross Harris and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of G1 Phase and Differentiation in Candida Albicans by the Cyclin CLn3p and MBF Transcription Factor Complex written by Bahira Hussein and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book G1 S Transcriptional Regulation in Saccharomyces Cerevisiae Integrates Cell Cycle Progression and Genome Stability written by and published by . This book was released on 2014 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Genetics of Recombination written by Andrés Aguilera and published by Springer Science & Business Media. This book was released on 2007-04-24 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.
Download or read book Systems Biology and Synthetic Biology written by Pengcheng Fu and published by John Wiley & Sons. This book was released on 2009-08-13 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The genomic revolution has opened up systematic investigations and engineering designs for various life forms. Systems biology and synthetic biology are emerging as two complementary approaches, which embody the breakthrough in biology and invite application of engineering principles. Systems Biology and Synthetic Biology emphasizes the similarity between biology and engineering at the system level, which is important for applying systems and engineering theories to biology problems. This book demonstrates to students, researchers, and industry that systems biology relies on synthetic biology technologies to study biological systems, while synthetic biology depends on knowledge obtained from systems biology approaches.
Download or read book DNA Replication written by Hisao Masai and published by Springer. This book was released on 2018-01-22 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the latest trends and future directions of DNA replication research. The contents reflect upon the principles that have been established through the genetic and enzymatic studies of bacterial, viral, and cellular replication during the past decades. The book begins with a historical overview of the studies on eukaryotic DNA replication by Professor Thomas Kelly, a pioneer of the field. The following chapters include genome-wide studies of replication origins and initiation factor binding, as well as the timing of DNA replications, mechanisms of initiation, DNA chain elongation and termination of DNA replication, the structural basis of functions of protein complexes responsible for execution of DNA replication, cell cycle-dependent regulation of DNA replication, the nature of replication stress and cells’ strategy to deal with the stress, and finally how all these phenomena are interconnected to genome instability and development of various diseases. By reviewing the existing concepts ranging from the old principles to the newest ideas, the book gives readers an opportunity to learn how the classical replication principles are now being modified and new concepts are being generated to explain how genome DNA replication is achieved with such high adaptability and plasticity. With the development of new methods including cryoelectron microscopy analyses of huge protein complexes, single molecular analyses of initiation and elongation of DNA replication, and total reconstitution of eukaryotic DNA replication with purified factors, the field is enjoying one of its most exciting moments, and this highly timely book conveys that excitement to all interested readers.
Download or read book Genetic and Epigenetic Mechanisms Regulating the G1 S Transcriptional Wave in Fission Yeast written by Alberto González Medina and published by . This book was released on 2019 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: The control of proper progression of eukaryotic cell cycle is essential if cells want to continue proliferating appropriately, and it is a conserve feature from yeast to human cells. One of the most important points of cell cycle control takes place at the end of the G1 phase, when cells are compromised between remaining in a state of quiescence (G0) or continuing the proliferative cycle independently of environmental signals. This point is known as Start in yeast and depends on the activation of the transcription factor MBF. In this thesis, we show that tRNA methyltransferases and the Elongator complex promote an accurate MBF activity and that COP9/Signasolome together the E3 ubiquitin ligase complex Cul4-Ddb1Cdt2 are responsible for the downregulation of MBF-dependent transcription. Furthermore, we have study the feature of the chromatin around MBF-regulated promoters, since the chromatin represent an impediment to the access of the transcription machinery. In this sense, we have found that the chromatin remodeler INO80 and the histone acetyltransferase Gcn5 interact with MBF in a cell cycle-dependent manner to promote the initiation of the transcription of MBF-regulated genes.
Download or read book Cell Cycle Regulation written by Philipp Kaldis and published by Results and Problems in Cell Differentiation. This book was released on 2006-06-26 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a state-of-the-art summary of the latest achievements in cell cycle control research with an outlook on the effect of these findings on cancer research. The chapters are written by internationally leading experts in the field. They provide an updated view on how the cell cycle is regulated in vivo, and about the involvement of cell cycle regulators in cancer.
Download or read book Cell Cycle Control written by Michele Pagano and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addressing the regulation of the eukaryotic cell cycle, this book brings together experts to cover all aspects of the field, clearly and unambiguously, delineating what is commonly accepted in the field from the problems that remain unsolved. It will thus appeal to a large audience: basic and clinical scientists involved in the study of cell growth, differentiation, senescence, apoptosis, and cancer, as well as graduates and postgraduates.
