Download or read book Transcriptional Regulation and Autogenous Control of the Genes for the and Subunits of Escherichia Coli RNA Polymerase microform written by Steward, Keith L., 1962 and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1994 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: The transcriptional control of the two genes rpoB and rpoC encoding the $\beta$ and $\beta\sp\prime$ subunits of RNA polymerase was examined in a systematic fashion in order to evaluate the contribution of such control to the autogenous regulation of RNA polymerase synthesis. Numerous transcriptional fusions were constructed by placing restriction fragments of the rplKaJLrpoBC gene cluster upstream of the lacZ reporter gene on $\lambda$ phage vectors. Assaying $\beta$-galactosidase activity from monolysogens of the recombinant phage permitted a high resolution scan of the gene cluster for transcriptional controls. The results precisely quantitate the efficiencies of two strong promoters, rplKp and rplJp, a weak promoter rplLp, and a transcriptional attenuator rpoBa located between rplL and rpoB. From this data, the relative contribution of each transcriptional signal to the overall transcription of rpoBC was determined. It was found that almost all transcription initiated from the strong rplKp promoter continued into the downstream rplJLrpoB genes; that rplJp continues to initiate efficiently despite such transcription from the upstream promoter; and most surprisingly, that the efficiency of termination at the rpoBa attenuator depended on whether transcription was initiated from rplKp, rplJp, or both promoters. Further examination of the latter phenomenon revealed that the efficiency of rpoBa was related in an inverse fashion to the frequency of transcription initiated by promoters located upstream of it. When the frequency of transcription into the attenuator was decreased over a 40-fold range by replacing rplJp and rplKp with alternative promoters, readthrough at rpoBa increased from 19% to 61%. When regions located upstream of rpoBa are deleted or inverted, the modulation of rpoBa may be abrogated. In addition, sequence analysis of the region can identify a site with remarkable homology to the boxB motif of the $\lambda\ nutR$ site. Such findings suggest the potential involvement of antitermination in the modulation of rpoBa function. Modulation of rpoBa, together with the evidence by others that the global frequency of transcription is influenced by the cellular concentration of RNA polymerase, suggests that rpoBa may play a role in the autogenous control of rpoBC expression.
Download or read book Transcriptional Regulation and Autogenous Control of the Genes for the L and L Subunits of Escherichia Coli RNA Polymerase written by Keith Leroy Steward and published by . This book was released on 1993 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Physiological and Regulatory Parameters Controlled by RNA Polymerase Concentration in Escherichia Coli written by David M. Bedwell and published by . This book was released on 1985 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Transcription of the Gene for the Sigma Subunit of E Coli RNA Polymerase by a Heat Shock Promoter written by Wayne Emery Taylor and published by . This book was released on 1983 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Studies on the Structure and Function of the sigma 70 Subunit of Escherichia Coli RNA Polymerase written by James Chi-Min Hu and published by . This book was released on 1987 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on the Function and Regulation of Two Sigma Subunits of Escherichia Coli RNA Polymerase written by Alan Davis Grossman and published by . This book was released on 1984 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Study of the Transcriptional Control Region written by Rabindra Nath Roy and published by . This book was released on 1982 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Post transcriptional Control of Gene Expression written by Orna Resnekov and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many important cellular processes rely on posttranscriptional control of gene expression. This book describes the mechanisms of gene expression at this level that occur in the cytoplasm of prokaryotes and eukaryotes. Several introductory chapters discuss the general principles of translation and mRNA stability. The interactions of mature mRNA with the translational machinery, the components of mRNA degradation and antisense RNA are surveyed. Subsequent chapters discuss protein folding, transport, modification and degradation. The book is an invaluable source of information for both newcomers and those wishing an overview of the field.
Download or read book Genetic Analysis of the beta Subunit of Escherichia Coli RNA Polymerase written by Patricia L. Tavormina and published by . This book was released on 1994 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Transcriptional Elongation in Escherichia Coli written by Rachel Anne Mooney and published by . This book was released on 2007 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanism of Promoter Recognition and Transcription Regulation by the Sigma S Subunit of Escherichia Coli RNA Polymerase written by and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Growth Rate Regulation and Control of Initiation of DNA Replication in Escherichia Coli written by Anne Eliane Chiaramello and published by . This book was released on 1990 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The initiation of DNA synthesis at the chromosomal replication origin, oriC, in Escherichia coli involves an RNA polymerase-mediated step. The level of synthesis of transcripts moving counterclockwise toward oriC is controlled at two promoters, P1 (asnC) and P2 (mioC), and at two transcription terminator regions, T1 and T2. As shown by S1 mapping, termination at the T2 region occurs to the right of oriC at nucleotides 297-299 and 306-310, while major termination events in the T1 region occur in and near the mioC promoter. The majority of transcripts entering oriC originates from the mioC promoter. Transcription from the mioC promoter has been shown to enhance the frequency of initiation of DNA replication of oriC containing plasmids, and to stabilize these plasmids in the host cells. The mioC promoter, which is stringently controlled, is also growth rate regulated. The amount of mioC transcripts relative to the amount of total RNA was inversely correlated with growth rate. This transcript is characterized by a short half-life (1.5 min). The mioC promoter, which contains a DnaA protein binding site, was much less susceptible to repression by DnaA protein when located in the chromosome, than when located in a plasmid. Only a very high concentration of DnaA protein repressed the mioC promoter. The DnaA protein, which is required for initiation of DNA replication from oriC, is growth rate regulated. As shown by RNase protection, this regulation is exerted at the transcriptional level, affecting both promoters, dnaAp1 and dnaAp2. Transcription from these two promoters is also stringently controlled. The amount of DnaA protein in spoT mutants, which are deficient in ppGpp pyrophosphorylase activity, decreases as the severity in the mutation increases. Thus, the intracellular concentration of ppGpp influences the expression of the dnaA gene. In conclusion, the growth rate regulation and stringent control of the dnaA gene suggest that one way in which DNA replication is coordinated with the growth rate is via ppGpp synthesis at the ribosome.
Download or read book Mechanism of Promoter Recognition and Transcription Regulation by the s Subunit of Escherichia Coli RNA Polymerase written by Stephan Lacour and published by . This book was released on 2003 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Ribosomal Protein and Ribosomal RNA Synthesis in Escherichia Coli written by Sue Jinks Robertson and published by . This book was released on 1983 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation written by Steven L. McKnight and published by . This book was released on 1992 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sequence Insertion 3 of Escherichia Coli RNA Polymerase Regulates Transcriptional Pausing written by Yu Bao (Ph.D.) and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene expression in all life forms relies on multi-subunit RNA polymerase (RNAP). In transcription elongation stage, RNAP senses specific DNA signals and enters an offline, pausing state, which has multiple interconverting subtypes and allows versatile regulatory options. This work focuses on understanding the pausing regulatory function of sequence insertion 3 (SI3) in Escherichia coli (E. coli) RNAP. In E. coli, the catalytic trigger loop (TL) is interrupted by surface-exposed, 188-aa sequence insertion 3 (SI3). SI3 occupies different locations in paused transcription complexes, but its dynamics during pausing are undefined. We report design, optimization, and use of a Cys-triplet reporter to measure the positional bias of SI3 in different transcription complexes and to determine the effect of restricting SI3 movement on pausing. RNA hairpin-stabilized pausing biases SI3 toward a swiveled position that inhibits TL folding while the consensus elemental pausing biases SI3 toward a closed position that potentially stabilized a pre-translocated state of RNAP. Our findings establish that SI3 functions by modulating the TL folding to aid transcriptional regulation. To investigate SI3's genomic function in transcriptional pausing, we constructed a viable E. coli strain lacking SI3 enabled by a suppressor TL substitution ([beta]'Ala941-Thr; [delta]SI3*). [delta]SI3* increased transcription rate in vitro relative to [delta]SI3, possibly explaining its viability, but retained both positive and negative effects of [delta]SI3 on pausing. Using NET-seq, we found that [delta]SI3*-enhanced pauses resemble the consensus elemental pause sequence whereas sequences at [delta]SI3*-suppressed pauses, which exhibited greater association with PHs, were more divergent. [delta]SI3*-suppressed pauses also were associated with apparent pausing one nt upstream from the consensus sequence, often generating tandem pause sites. Our results document multiple ways that SI3 modulates pausing in vivo and may explain differences in consensus pause sequences found in some NET-seq studies. In conclusion, our comprehensive studies provide compelling evidence linking the movements of the SI3 domain to the formation and stabilization of diverse transcriptional pause signals. We demonstrate the potential of biochemical crosslink assays in exploring conformational changes within large protein complexes. Additionally, our research emphasizes the necessity for refining current NET-seq protocols to prevent unintended cleavage during library preparation.
Download or read book Mechanisms of Gene Expression written by Robert O. J. Weinzierl and published by World Scientific Publishing Company Incorporated. This book was released on 1999 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book presents much of the current thinking concerning molecular mechanisms of transcriptional control in a form easily accessible to undergraduates with an understanding of basic molecular biology concepts. It contains detailed information about the various pro- and eukaryotic transcriptional machineries that has recently become available through the combined efforts of geneticists, biochemists and structural biologists. The book will thus not only serve as an undergraduate text but also offer something new and interesting to more advanced readers and professional scientists who want to keep up to date with rapid advances in this field."--BOOK JACKET.Title Summary field provided by Blackwell North America, Inc. All Rights Reserved
