Download or read book Total Synthesis and Expression of the Msp 1 Gene of Malaria Parasite Plasmodium Falciparum Encoding the Merozoite Surface Protein 1 of the FCB 1 Strain written by Weiqing Pan and published by . This book was released on 1999 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Production of a Recombinant E Coli Expressed Malaria Vaccine from the C Terminal Fragment of Piasmodium Falciparum 3D7 Merozoite Surface Protein 1 written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malarial blood stage antigens can produce humoral immunity by induction of protective antibodies to epitopes on surface antigens on parasites. The merozoite surface protein-1 (MSP1) of P. falciparum is a major blood-stage vaccine candidate. MSP1 may play a role in binding and/or infection of erythrocytes by merozoites (parasites) during red blood cell invasion. The C-terminal fragment of MSP1, MSP1(sub 19), is highly conserved among all known strains of P. falciparum. immunization with recombinant proteins that have native-like conformations may elicit long-lasting protective immunity that mimics the natural immunity. We have used bacterial expression to tightly regulate transcription and translation of MSP1(sub 42). Plasmids that encode MSP1(sub 42) were prepared and tested for their ability to express recombinant protein. A three column purification scheme that included Ni(+2) metal chelate chromatography, anion and then cation exchange chromatography, yielded greater than 95% pure MSP1(sub 42). Immunoreactivity with mAbs showed that recombinant MSP1(sub 42) was conformationally similar to native MSP1. The objectives were to develop recombinant MSP1(sub 42) molecules that were structurally correct, to develop fermentation and purification processes that could be scaled-up for large-scale processes, and ultimately, to advance these products into human clinical trials.
Download or read book Malaria Parasites written by Andrew P. Waters and published by Caister Academic Press Limited. This book was released on 2004 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: The completion of the Plasmodium falciparum genome sequence in late 2002 heralded a new era in malaria research. The search began in earnest for new drugs and vaccines to combat malaria, a disease which afflicts up to 500 million people worldwide and is responsible for the deaths of more than one million people each year. The new genomic data is aiding a greater understanding of the living parasite and its interaction with the insect vector and human host. In this book internationally renowned experts provide up-to-date reviews of the most important aspects of post-genomic malaria research. Topics covered include: the P. falciparum genome and model parasites, bioinformatics and genome databases, microsatellite analysis, analysis of chromosome structure, cell cycle to RNA polymerase I and II mediated gene expression, role of the nuclear genome, the parasite surface and cell biology, and much more. The book is essential to all researchers working in this highly topical field and is recommended reading for scientists in other areas of biology and medicine.
Download or read book Production of a Recombinant E Coli Expressed Malarial Vaccine from the C Terminal Fragment of Plasmodium Falciparum 3D7 Merozoite Surface Protein 1 written by and published by . This book was released on 2000 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt: P. falciparum Merozoite Surface Protein-1 (MSP-1) is a leading erythrocytic stage vaccine candidate. Following secondary proteolytic processing, it may play a role in binding and/or invasion of erythrocytes. The secondary processing gives a 33kD and 19kD fragment. The MSP1-19 is a target for parasite inhibitory antibodies and protective immunity. However, it appears to lack T-helper epitopes. Since antibody is likely the effector mechanism induced by MSP1-19, it is important to insure that recombinant vaccines based on this antigen be folded correctly and contain T-helper epitopes that will enhance induction of humoral responses. We have developed recombinant MSP1-42 molecules that are structurally correct based on positive reactivities with conformation-dependent mAbs. These proteins have been produced to greater than 95% purity and exceed FDA endotoxin standards. Immunogenicity studies in mice and Rhesus monkeys reveal that good antibody responses are induced to MSP1-42 when these animals are immunized with the MSP1-42 plus SBAS2 adjuvant.
Download or read book Detection of the Merozoite Surface Protein MSP 1 Gene from Different Malarial Isolates by Polymerase Chain Reaction PCR written by Lader Charlotte Simone and published by . This book was released on 1996 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Characterization of a Malaria Vaccine Candidate written by Fei Fei Miu and published by . This book was released on 2003 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Malaria Drugs Disease and Post genomic Biology written by David Sullivan and published by Springer Science & Business Media. This book was released on 2006-01-09 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite rapid increases in knowledge, malaria continues to kill more than a million people each year and causes symptomatic disease in a further 300 million individuals. This volume brings some of the world's best investigators to describe recent advances in both the scientific and clinical aspects of malaria, and bridges between the two.
Download or read book Meningococcal Vaccines written by Andrew J. Pollard and published by Springer Science & Business Media. This book was released on 2001-06-25 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus, and to develop, evaluate, and implement successful new meningococcal vaccines.
Download or read book Tropical Pathology written by W. Doerr and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1338 pages. Available in PDF, EPUB and Kindle. Book excerpt: The continuous and rapid improvement of tourism around the whole world and the increasing emigration of peoples from the developing countries to the "old continents" have changed the classical image of tropical diseases, which are now seen more frequently in temperate and highly developed countries. Consequently, over 10 years after its first publication, this second edition of the pathology of tropical diseases has been restructured and expanded to reflect the recent developments and changes in diagnostic techniques. A total of 27 chapters - written by 29 reputed experts from 11 countries - cover such new aspects as technological developments in diagnosing infectious diseases, autopsies in the tropics, renal diseases, geomedicine and genetic disorders. A concentrated and up-to-date review of the field.
Download or read book Abstracts of Publications written by National Science Foundation (U.S.). Division of Intergovernmental Science & Public Technology and published by . This book was released on 1967 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizes the publications that have resulted from the activities that have been sponsored in State and local governments and in technologically-oriented institutions around the country in the Intergovernmental Science Program.
Download or read book Aging Methods and Protocols written by Yvonne A. Barnett and published by Humana. This book was released on 2010-11-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aging is an almost universal process within biological systems, one which leads to a decline in functional capacity, disease onset, and eventually death. There has been much interest in recent years to elucidate the molecular mec- nisms that underlie the aging process. Many theories have been proposed since the last century that aim to explain the causes of aging. There is no one theory that completely satisfies the phenotype of aging, but genetics and environm- tal factors play an important role in the etiology of age-related pathologies and the aging process. However, there is still much to be learned about the aging process which has been termed one of the last great frontiers in biology. De- graphic changes worldwide are leading to increased average life expectancies within our populations. These changes in population characteristics will impact upon the economies of the supporting society, with increasing healthcare and infrastructural costs arising from the prevalence of age-related pathologies and other physical disabilities associated with advancing years. Many researchers worldwide are working in the attempt to identify key cellular processes through which it might one day be possible to slow down the aging process and thus increase the health span of humans. Numerous research projects—from the cellular through to tissue, organ, and whole organism studies—are currently underway to investigate the mul- factorial aging process.
Download or read book Psychiatric Genetics written by Marion Leboyer and published by Springer Science & Business Media. This book was released on 2003 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the reader with a complete view of the methodological problems encountered in psychiatry genetics and proposes solutions to commonly occurring questions. Specialists give a thorough review on the advantages and disadvantages of genetic marker or a clinical interview and how to ascertain patients, unaffected relatives and controls and what should be the criteria to include a case or a control.
Download or read book Food Borne Parasitic Zoonoses written by K. Darwin Murrell and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Humans suffer from numerous parasitic foodborne zoonoses, many of which are caused by helminths. The helminth zoonoses of concern in this book were once limited to diseases of animals, but have now become transmissible to humans. This book reviews not only the prevalence and distribution of these zoonoses, including available health and economic impact data, but highlights gaps in our knowledge that must be filled in order to assess the importance of a particular zoonosis.
Download or read book Clinical Tropical Medicine written by Kevin M. Cahill and published by . This book was released on 1972 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Angiogenesis Protocols written by J. Clifford Murray and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few years, we have been deluged with information on ang- genesis. Scientists and the public at large are exposed daily to this “new” science, not just in specialist journals and texts, but in the tabloid press, where popular articles refer to angiogenic therapies as magic bullets and miracle cures for cancer, arthritis, retinopathies, heart disease, and circulatory problems. Is there no ill this approach will not cure? The fact that so much time, effort, and resource have been and continue to be dedicated to this new science is clear testament to its importance. Yet many fundamental aspects of angiogenesis remain poorly und- stood, in particular cues that activate the process. This fact has to some extent been masked behind a surfeit of fine detail; we can’t see the wood for the trees. Most studies of angiogenesis identify single links in a long chain of events. Furthermore, each study is itself hampered by the limitations of the biological end-point chosen. For instance, though endothelial proliferation may well be necessary for angiogenesis, it is not sufficient. Therefore, measuring endothelial proliferation in response to a novel growth factor, and on the basis of this obs- vation, stating that the factor is “angiogenic,” is unsound logic. It is important that researchers in this field, and perhaps more importantly those experimenting at its periphery, recognize the limitations of their chosen biological end-points.
