Download or read book The Systems Biology of Red Cell Metabolism written by James T Yurkovich and published by . This book was released on 2018 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human red blood cell (RBC) is a logical starting point for the development and application of systems biology methods because of its simplicity, intrinsic experimental accessibility, and importance in human health. New "-omics" technologies have been used to study the biochemical and morphological changes that occur in red blood cells during cold storage, collectively referred to as the "storage lesion." Here, we extend these previous efforts by using systems biology to examine the metabolic physiology of RBCs under storage conditions. We first characterized the temperature dependence of the storage process using previously identified storage-age biomarkers as a representation of systems-level trends, showing that the metabolic state of the RBC is conserved but accelerated with increasing temperature. We then questioned whether these biomarkers--which had been shown to be excellent qualitative markers of systemic behavior--held any potential to provide quantitative information about the system. Using simple linear statistical models, we showed that a subset of the biomarkers could be used to predict the quantitative concentration profiles of other metabolites in the RBC network. We expanded these efforts by integrating network structural information into these statistical models to forecast future values of these concentration profiles after measurements made during only the first eight days of storage. Next, we used multiple first principles modeling approaches to understand the underlying mechanisms and temporal dynamics of the observed behaviors and developed a method for the integration of metabolomics data into cell-scale mathematical models. Finally, we developed a method for the integration of quantitative proteomics data into cell-scale models using Escherichia coli as a test case. Collectively, these results provide empirical proof that the RBC metabolome can be represented in a low-dimensional space and offer the starting point for a whole-cell model of the RBC. More broadly, we detail the development and use of systems biology methods on the human RBC, providing a starting point from which we can expand these efforts to other, more complicated cellular systems.
Download or read book Red Cell Metabolism and Function written by George Brewer and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last six years, a remarkable series of stUdies have demonstrated an intimate relationship between red cell metabolism and the function of the cell as an organ of gas transport. First came the demonstration of binding of organic phosphocompounds of the red cell to hemoglobin; this was followed by studies that demonstrated modification of hemoglobin oxygen affinity by such binding. At present we are in an exhilirating phase of accrual of data showing that the levels of these phosphorylated inter mediates can be rapidly altered in the red cell to modulate hemo globin function. At one time it was said that the red cell was an inert bag full of hemoglobin. Now we know not only that the cell has an active metabolism crucial to its viability, but that this metabolism is just as crucial to the whole organism in the proper adjustment of oxygen transport. On October first, second and third, 1969, red cell biochemists, general biochemists, geneticists, cardio-pulmonary physiologists, exercise physiologists, experts in blood storage, and represen tatives from many other disciplines met in the Towsley Center for Continuing Medical Education at the University of Michigan, Ann Arbor, to present recent findings and discuss developments in this new interdisciplinary field. The meeting was dedicated to Dr. Alfred Chanutin, Professor Emeritus of the University of Virginia, to honor his retirement in 1967 and in recognition of his great contributions to the studies outlined in the first paragraph of this preface.
Download or read book A Systems Biology Approach to Blood written by Seth Joel Corey and published by Springer. This book was released on 2014-12-06 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: The blood system is multi-scale, from the organism to the organs to cells to intracellular signaling pathways to macromolecule interactions. Blood consists of circulating cells, cellular fragments (platelets and microparticles), and plasma macromolecules. Blood cells and their fragments result from a highly-ordered process, hematopoiesis. Definitive hematopoiesis occurs in the bone marrow, where pluripotential stem cells give rise to multiple lineages of highly specialized cells. Highly-productive and continuously regenerative, hematopoiesis requires a microenvironment of mesenchymal cells and blood vessels. A Systems Biology Approach to Blood is divided into three main sections: basic components, physiological processes, and clinical applications. Using blood as a window, one can study health and disease through this unique tool box with reactive biological fluids that mirrors the prevailing hemodynamics of the vessel walls and the various blood cell types. Many blood diseases, rare and common can and have been exploited using systems biology approaches with successful results and therefore ideal models for systems medicine. More importantly, hematopoiesis offers one of the best studied systems with insight into stem cell biology, cellular interaction, development; linage programing and reprograming that are every day influenced by the most mature and understood regulatory networks.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Red Cell written by George J. Brewer and published by . This book was released on 1981 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book E Cell System written by Satya Nanda Vel Arjunan and published by Springer Science & Business Media. This book was released on 2013-05-13 with total page 185 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interdisciplinary field of molecular systems biology aims to understand the behavior and mechanisms of biological processes composed of individual molecular components. As we gain more qualitative and quantitative information of complex intracellular processes, biochemical modeling and simulation become indispensable not only to uncover the molecular mechanisms of the processes, but to perform useful predictions. To this end, the E‐Cell System, a multi‐algorithm, multi‐timescale object‐oriented simulation platform, can be used to construct predictive virtual biological systems. Gene regulatory and biochemical networks that constitute a sub‐ or a whole cellular system can be constructed using the E‐Cell System to perform qualitative and quantitative analyses. The purpose of E‐Cell System: Basic Concepts and Applications is to provide a comprehensive guide for the E‐Cell System version 3 in terms of the software features and its usage. While the publicly available E‐Cell Simulation Environment version 3 User's Manual provides the technical details of model building and scripting, it does not describe some of the underlying concepts of the E‐Cell System. The first part of the book addresses this issue by providing the basic concepts of modeling and simulation with the E‐Cell System.
Download or read book Red Cell Metabolism written by Ernest Beutler and published by Grune & Stratton, Incorporated. This book was released on 1971 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Red Cell Metabolism and Function written by George Brewer and published by . This book was released on 1995-12-31 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantitative Biology of Metabolism written by Alfred Locker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of the life sciences may be said to have effected a gradual transition from a more or less intuitive prescientific approach based on crude observation, via a more refined type of observation to experimentation and hence to the level of formal theories. Quantitative methods are introduced at the second level; they comprise: (a) quantitative design of experiments; (b) regrouping of experimental results; (c) evalua tion of results by means of mathematical or special statistical techniques. The last step implies the introduction of theoretical concepts, but we are not justified in speaking of theoretical science unless true theoretical con siderations-models or hypotheses-precede experiment, and this is then followed by an attempt to link results with theory so as to verify the theory. Biology at present seems to lie somewhere between the second and third level mentioned above, not yet having achieved the status of a theoretical science in all its branches. Thus, though the need for quantification and mathematical formulation is widely recognized, many biologists still believe that e.g. general systems theory is too abstract to be of use in handling concrete problems. Those, however, who look critically at the present state of affairs cannot adopt this attitude.
Download or read book Red Cell Metabolism and Function written by George J. Brewer and published by . This book was released on 1970 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Phosphate and Mineral Metabolism written by Shaul G. Massry and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present to our readers the Proceedings of the Fifth International Workshop on Phosphate and Other Minerals which was held in New York City, New York, U.S.A during September 23-27,1981. It was hosted by Joseph M. Letteri, M.D., Professor of Medicine at the State University of New York at Stonybrook School of Medicine, and Chief, Division of Nephrology, Nassau County Medical Center. As in the previous Workshops, this meeting provided an oppor tunity for interested scientists from interrelated fields, including nephrology, endocrinology, physiology, biochemistry and nutrition, to get together and discuss the recent advances in the field of phosphate and mineral metabolism. There were 29 invited presenta tions by leading scientists and 40 oral and 90 poster presentations selected from over 250 abstracts submitted to the Organizing Committee. The Workshop was attended by 250 scientists from 14 countries including Austria, Australia, Canada, Denmark, England, France, Germany, Holland, Israel, Italy, Japan, Sweden, and the United States of America. The Sixth International Workshop on Phosphate and Other Minerals will be held during June 24-26, 1983 in Verona, Italy. It will be hosted by Professor Giuseppe Maschio, Director, Division of Nephrology, Istituti Ospitalieri, Verona, Italy. The theme of this coming Workshop will continue to focus on the pathophysiology of phosphate homeostasis and the metabolism of other minerals.
Download or read book Red Cell Metabolism and Function written by International Conference on Red Cell Metabolism and Function and published by . This book was released on 1970 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Red Cell Metabolism and Function written by and published by . This book was released on 1970 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biology for AP Courses written by Julianne Zedalis and published by . This book was released on 2017-10-16 with total page 1923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences.
Download or read book A Systems Biology Approach to Study Metabolic Syndrome written by Matej Orešič and published by Springer Science & Business Media. This book was released on 2013-09-24 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to provide the target audience, specifically students of Medicine, Biology, Systems Biology and Bioinformatics, as well as experienced researchers in research fields relevant to metabolic syndrome (MetS) with an overview of the challenges and opportunities in systems biology and how it can be used to tackle MetS. In particular, the aims are: (1) to provide an introduction to the key biological processes involved in the pathophysiology of MetS; (2) through the use of specific examples, provide an introduction to the latest technologies that use a systems biology approach to study MetS; and (3) to give an overview of the mathematical modeling approaches for studying MetS. The clearly written chapters by leading experts in the field provides detailed descriptions crucial for the unique position of this book and its focus on the application of systems biology to tackle specific pathophysiologically relevant aspects of MetS and provides a valuable practical guide to this research community.
Download or read book Concepts of Biology written by Samantha Fowler and published by . This book was released on 2018-01-07 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.
Download or read book High throughput Metabolomics to Understand Red Blood Cell Responses to Storage and Hypoxia written by Travis Nemkov and published by . This book was released on 2017 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical investigations into the regulatory mechanisms of red blood cell metabolism have fostered a century of advances in the field of transfusion medicine. Resulting from these advances, the storage of packed red blood cell concentrates has become a life-saving practice in clinical and military settings. However, improvements to storage quality are still necessary and achievable through the implementation of novel storage and metabolic rejuvenation media, alternative cell processing strategies, and improved storage conditions. Recent advancements in mass spectrometry-based metabolomics and bioinformatics integration of big data hold promise for accelerating the development of innovative storage strategies that target the improvement of quality, safety, and efficacy of transfused blood products. To improve understanding of red blood cell responses to storage and environmental stimuli such as hypoxia, sample sets with robust spatial and temporal sampling must be analyzed. As such, this dissertation describes the design and optimization of high throughput metabolomics techniques to improve characterization of the storage lesion in various storage media, referred to as additive solutions, as well as red blood cell responses to high altitude hypoxia and analogous responses to induced hypoxia during blood bank storage. The application of these methods has identified storage day 14 as an inflection point where substantial biochemical alterations to red blood cell metabolism manifest when stored in a commonly used additive solution in the United States, Additive Solution 3 (AS-3). As much of the storage lesion results from progressive intracellular acidification due to excess lactate production and deviations from cellular ion homeostasis, we then show that storage in a low-salt, alkaline additive solution 7 (AS-7) confers improved energy and redox metabolism during at least the first two weeks of storage. Furthermore, these studies have identified a catabolic pathway for the AS-3 component citrate that may have beneficial effects on storage quality. By studying red blood cell metabolic adaptations to high altitude hypoxia, we have substantiated, for the first time in vivo, a model for oxygen-dependent metabolic modulation of red blood cells. These findings support the hypothesis that hypoxic storage of red blood cells may promote energy metabolism while mitigating oxidative stress, thus improving storage quality. We further demonstrate that this response to storage hypoxia can be used to prospectively improve the quality of red blood cell storage through the monitoring of novel storage biomarkers, which are described herein. These findings ultimately enable investigation of correlative and mechanistic relationships among metabolic variables and transfusion-relevant parameters, such as 24-hour in vivo recovery for transfused red blood cells. Integration with systems biology models will potentially allow for in silico prediction of metabolic phenotypes, thus streamlining the design and testing of alternative storage strategies and solutions to improve outcomes of transfusion, which serves as the second-most utilized medical procedure in the United States.
