Download or read book The Surfaceome written by Kenneth R. Boheler and published by Humana Press. This book was released on 2018-08-30 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
Download or read book The Surfaceome written by Kenneth R. Boheler and published by Humana Press. This book was released on 2017-12-20 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
Download or read book Mass Spectrometry Based Proteomics written by Kris Gevaert and published by Springer Nature. This book was released on 2023-09-04 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Proteomics serves as an ideal guide to its subject for both novices in the field of proteomics as well as specialists.
Download or read book Senolytics in Disease Ageing and Longevity written by Daniel Muñoz-Espin and published by Springer Nature. This book was released on 2020-04-27 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the new and rapidly evolving science of identifying and targeting senescent cells, and on the exciting prospect of new diagnostic and therapeutic opportunities for stopping, and even reversing, the progression of disease and the deterioration of the human body due to ageing. According to recent United Nations data, by 2050 one in six people worldwide will be older than age 65, with peaks rising to one in four people in Europe and North America. Remarkably, the number of persons aged 80 years or older is expected to triple, from 143 million in 2019 to 426 million in 2050. First documented in the 1960s, the concept of cellular senescence as an underlying cause of ageing has been established in the course of the last decade. Using genetically engineered mouse models, researchers have demonstrated that the selective elimination of senescent cells can block and even reverse a number of age-related dysfunctions and pathologies, promoting both better health and longer life in the elderly. These include cardiovascular diseases; neurological disorders; type 1 and type 2 diabetes; inflammatory diseases; fibrosis; geriatric syndromes; chronic diseases resulting in organ dysfunction; the integrity of the musculoskeletal system; and cancer. Some senolytic agents have already progressed into trials. These include UBX0101 for the treatment of osteoarthritis (now in phase II), a cocktail of dasatinib and quercetin for the management of idiopathic pulmonary fibrosis and chronic kidney disease, and ABT-263 in combination with senescence-inducing chemotherapies for the treatment of advanced solid tumours. In addition, the book discusses pathways to early phase clinical trials and translational approaches in medicine and ageing, highlighting new opportunities as well as current limitations, challenges and alternatives. Given its scope, it will benefit a broad audience of advanced educators, researchers, graduate students and practitioners.
Download or read book Proteomics Approaches to Unravel Virus Vertebrate Host Interactions written by and published by Academic Press. This book was released on 2021-04-30 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions, Volume 109 in the Advances in Virus Research series, highlights state-of-the art mass spectrometry techniques to elucidate the tight interplay of vertebrate viruses and their host cells. The volume includes chapters on Spatio-temporal resolution of host protein complexes during virus entry, Proteomic approaches to investigate gammaherpesvirus biology and associated tumorigenesis, Applications of Mass Spectrometry Imaging in Virus Research, Mapping surfaceome dynamics during viral infection, Characterization of proteolytic events in virus-host interactions, Dynamic protein network modulation upon viral infection, and much more. Discusses the latest methodological breakthroughs in mass spectrometry-based proteomics Reviews how technology has advanced our knowledge on virus-host interactions Provides future perspectives on proteomics research in virology
Download or read book Rheumatology E Book written by Marc C. Hochberg and published by Elsevier Health Sciences. This book was released on 2018-01-08 with total page 6453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thoroughly revised and updated, the 7th Edition of Rheumatology remains a leading text in this fast-changing field, keeping you abreast of recent advances in medications, therapies, clinical trials, and much more. Dr. Mark Hochberg and his team of expert authors and editors cover everything you need to know -- from basic scientific principles to practical clinical management strategies, all in a user-friendly, accessible manner. Provides access to quick, concise videos depicting musculoskeletal ultrasound including anisotropy, "comet tail" needle artifact, rheumatoid arthritis synovitis, acute gout, and more. Uses a consistent, logical, reader-friendly format with templated content and large-scale images for efficient visual reference. Features 14 completely new chapters covering biomedical and translational science, disease and outcome assessment, including new imaging modalities and early emerging disease, clinical therapeutics, and patient management and rehabilitation. Includes updated content on the latest diagnostic perspectives and approaches to therapy including tapering down of treatment, pulmonary hypertension and scleroderma, psoriatic arthritis, and the latest oral anticoagulants. Covers hot topics such as pathogenesis and pathophysiology of axial spondyloarthritis, DXA and measurement of bone, infectious arthritis, and more. Shares the knowledge and expertise of internationally recognized rheumatoid arthritis expert and new editor, Dr. Ellen Gravallese. Expert ConsultTM eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, and references from the book on a variety of devices.
Download or read book Food Safety written by Steven Ricke and published by Academic Press. This book was released on 2015-07-08 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food Safety: Emerging Issues, Technologies and Systems offers a systems approach to learning how to understand and address some of the major complex issues that have emerged in the food industry. The book is broad in coverage and provides a foundation for a practical understanding in food safety initiatives and safety rules, how to deal with whole-chain traceability issues, handling complex computer systems and data, foodborne pathogen detection, production and processing compliance issues, safety education, and more. Recent scientific industry developments are written by experts in the field and explained in a manner to improve awareness, education and communication of these issues. Examines effective control measures and molecular techniques for understanding specific pathogens Presents GFSI implementation concepts and issues to aid in implementation Demonstrates how operation processes can achieve a specific level of microbial reduction in food Offers tools for validating microbial data collected during processing to reduce or eliminate microorganisms in foods
Download or read book Improving Responses to Immunotherapy in Glioblastoma Multiforme written by Stephanie E. B. McArdle and published by Frontiers Media SA. This book was released on 2024-04-29 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glioblastoma multiforme (GBM) is a uniformly fatal primary brain tumour. Whilst many therapeutic interventions have been studied pre-clinically very few new therapies have been approved for the use of GBM therapy. These tumours present a unique challenge due to their location within the brain, making delivery of therapeutic interventions challenging. Active immunotherapy represents an attractive avenue for therapy, with activated immune cells being shown to cross the blood brain barrier and penetrate brain tumours. Despite several advances being made in numerous other cancer types, many immunotherapeutic interventions in GBM have failed to make it past phase 3 clinical trials and as of now (2022) no immunotherapeutic interventions have been approved for the therapy of GBM.
Download or read book Proteomics for Studying Foodborne Microorganisms and Their Impact on Food Quality and Human Health written by Rosa Anna Siciliano and published by Frontiers Media SA. This book was released on 2019-10-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foodborne microorganisms play a pivotal role in mediating the tight relation between food and human health from a dual perspective. In fact, notwithstanding the introduction of strict regulations and new technologies to ensure food quality and safety, foodborne pathogens continue to cause infections and diseases and represent a serious public health concern, while spoilage bacteria can severely affect food quality thus leading to major industry and commercial losses. On the other hand, probiotics positively affect human health, promoting digestion and uptake of dietary nutrients, strengthening intestinal barrier function, modulating immune response and enhancing antagonism towards pathogens. Nowadays, proteomics represents a key discipline to perform high-throughput studies in the field of food microbiology and enables the most accurate identification of complex networks of proteins involved in the cell adaptation to different growth conditions as well as in beneficial or harmful effects on human health. The present eBook offers an overview of the most up to date proteomic methodologies and further assesses the strength of proteomics in exploring different aspects of the foodborne microorganism life-style and defining biomarkers of food quality and safety.
Download or read book Advances in Animal Disease Diagnosis written by Suresh Kumar Gahlawat and published by CRC Press. This book was released on 2021-06-15 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Animal Disease Diagnosis: Infectious animal diseases caused by pathogenic microorganisms such as bacteria, fungi, and viruses threaten the health and well-being of wildlife, livestock and human populations, limit productivity and significantly increase economic losses to each sector. Pathogen de-tection is an important step for the diagnosis and successful treatment of animal diseases as well as control management in farm and field conditions. The conventional techniques employed to diagnose pathogens in livestock species are time-consuming and sometimes give inconclusive results. On the contrary, molecular techniques have the potential to diag-nose known pathogens/conditions quickly, reliably, and unequivocally as well as for novel pathogen detection. New advances in diagnostics and vaccine design using genomics have developed powerful new methods that have also set the stage for the enhanced diagnosis, surveillance, and control of infectious diseases. High-throughput sequencing (HTS), for ex-ample, uses the latest DNA sequencing platforms in the detection, identification, and detailed analysis of both pathogen and host genomes. This book will explore some key opportunities in the context of animal health, such as the detection of new microorganisms and the development of improved diagnosis of emerging or re-emerging diseases and other clinical conditions, viz. biosensors, nanotools, and omics technologies. Features • Details comprehensive knowledge on the latest molecular techniques for animal disease diagnosis and management • Examines how DNA-based diagnostic techniques will assist international efforts to control the introduction of exotic diseases into new geographic areas • Describes the latest molecular assays for the rapid and accurate detection of pathogens • Helps in working towards meeting the global challenge for sustainable food production and the eradication of poverty • With new biotechnological developments, this fully updated book is a treasure trove of the latest information in animal and medical science
Download or read book iPSC Derived Progenitors written by Alexander Birbrair and published by Academic Press. This book was released on 2021-11-10 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: iPSC Derived Progenitors, Volume Thirteen in the Advances in Stem Cell Biology series is a timely collection of information and new discoveries in the field of stem cell biology. The book addresses the importance of induced pluripotent stems cells and how can they be differentiated into different progenitors. Progenitors cells are often the first-step to making more differentiating cell types. This volume addresses iPSCs derived from bone, dental pulp, craniofacial, neural stem cells, otic, cardiac, and much more. The volume is written for researchers and scientists in stem cell therapy, cell biology, regenerative medicine, organ transplantation, and is contributed by world-renowned authors. Provides an overview of the fast-moving field of stem cell biology and function, regenerative medicine and therapeutics Covers how iPSCs can be differentiated into different progenitors Contributed by world renowned experts in the field
Download or read book Novel Insights into Algal Biology and Biotechnology written by Yuval Kaye and published by Frontiers Media SA. This book was released on 2019-05-23 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Retinal Degenerative Diseases written by John D. Ash and published by Springer Science & Business Media. This book was released on 2014-03-24 with total page 841 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will contain the proceedings of the XV International Symposium on Retinal Degeneration (RD2012). A majority of those who will speak and present posters at the meeting will contribute to this volume. The blinding diseases of inherited retinal degenerations have no treatments, and age-related macular degeneration has no cures, despite the fact that it is an epidemic among the elderly, with 1 in 3-4 affected by the age of 70. The RD Symposium will focus on the exciting new developments aimed at understanding these diseases and providing therapies for them. Since most major scientists in the field of retinal degenerations attend the biennial RD Symposia, they are known by most as the “best” and “most important” meetings in the field. The volume will present representative state-of-the-art research in almost all areas of retinal degenerations, ranging from cytopathologic, physiologic, diagnostic and clinical aspects; animal models; mechanisms of cell death; candidate genes, cloning, mapping and other aspects of molecular genetics; and developing potential therapeutic measures such as gene therapy and neuroprotective agents for potential pharmaceutical therapy. While advances in these areas of retinal degenerations will be described, there will be many new topics that either were in their infancy or did not exist at the time of the last RD Symposium, RD2010. These include the role of inflammation and immunity, as well as other basic mechanisms, in age-related macular degeneration, several new aspects of gene therapy, and revolutionary new imaging and functional testing that will have a huge impact on the diagnosis and following the course of retinal degenerations, as well as to provide new quantitative endpoints for clinical trials. The retina is an approachable part of the central nervous system (CNS), and there is a major interest in neuroprotective and gene therapy for CNS diseases and neurodegenerations, in general. It should be noted that with successful and exciting initial clinical trials in neuroprotective and gene therapy, including the restoration of sight in blind children, the retinal degeneration therapies are leading the way towards new therapeutic measures for neurodegenerations of the CNS. Many of the successes recently reported in these areas of retinal degeneration sprang from collaborations established at previous RD Symposia, and many of those will be reported at the RD2010 meeting and included in the proposed volume. We anticipate the excitement of those working in the field and those afflicted with retinal degenerations will be reflected in the volume.
Download or read book New Advances in Identification and Quantification of Foodborne Pathogens written by Dario de Medici and published by Frontiers Media SA. This book was released on 2022-01-03 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Insights Into New Strategies to Combat Biofilms written by Sujogya Kumar Panda and published by Frontiers Media SA. This book was released on 2021-11-09 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology and Treatment Strategies for Gliomas written by Terry Lichtor and published by BoD – Books on Demand. This book was released on 2024-01-17 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: A dramatic increase in knowledge regarding the molecular biology of gliomas has been established over the past few years, and this has led to the development of novel therapeutic strategies for these patients. This book describes some improvements in the surgical management of gliomas, including segmentation of brain MRI images using 4D MRI volumes to help with the diagnosis and monitoring of patients. Another novel topic reviewed involves the applications of photosensitizers and their efficacy in the generation of anti-tumor responses in photodynamic therapy. A review of the application of nanoparticles and their ability to deliver drugs to the tumor site with a reduction in systemic toxicity is another developing therapy discussed. The book also describes novel approaches involving the development of the use of microRNAs, which are non-coding RNAs that can be used as tumor suppressors that potentially can be developed to control the growth of gliomas. The book examines a large number of molecular interactions of signals in gliomas, which should lead to biomarkers of potential importance that could be manipulated in the development of clinical trials. Molecular networks need to be better understood for the development of therapeutic strategies. Finally, the book reviews immunotherapeutic strategies potentially useful in treating brain tumors that involve either poxviruses engineered to secrete IL-15- or IL-2-secreting fibroblasts transfected with tumor DNA. The stimulation of the immune system to selectively attack malignant cells should lead to the prolongation of survival of brain tumor patients without a decline in cognitive functions or other side effects. It is hoped that this new information will lead to improved and efficacious treatment strategies for these challenging tumors.
