Download or read book The Single stranded DNA Phages written by David T. Denhardt and published by . This book was released on 1978 with total page 746 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Stranded DNA Phages written by and published by . This book was released on 1978 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Replication and Packing of DNA in the Icosaherdral Single stranded DNA Phages written by Kirston Edward Koths and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Investigating the Diversity of Single Stranded DNA Bacteriophages in Marine Environments written by Max Stephen Hopkins and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The data was aggregated in several informative ways. Multiple alignments were combined with a predicted 3D-structure to reveal regions of both high and low conservation. Viewed in a phylogenetic framework, many gokushovirus MCP clades contained samples from multiple environments, although distinct clades dominated the different sample types. Some environments, particularly pelagic sediments, appear as hotbeds of gokushovirus diversity, while freshwater springs were the least diverse. The second chapter used the same primer set to detect gokushovirus communities at 0 m and 100 m depth in two seasons from three years at the Bermuda Atlantic Time- series Study (BATS) site. As a result of twenty-six years of constant sampling, the annual hydrodynamic cycling of BATS is very well understood. This wealth of knowledge allows us to hypothesize that the winter deep mixing layer will act to connect the viral communities between 0 m and 100 m. Conversely, in summer when stratification occurs, viral communities at the two depths will become divergent. We find compelling evidence to support this hypothesis. The final chapter of this thesis details continuing efforts to characterize the first non-tailed, single-stranded DNA, temperate phage to infect a member of the globally important genus of marine autotroph, Synechococcus. Efforts undertaken have spanned genomic, metagenomic and proteomic methodologies. The lack of culturable, phage-host model systems for small, single-stranded DNA phages is today one of the most glaring impediments to increased understanding of these viruses. In combination with the data presented on environmental diversity, steps taken towards establishing this Synechococcus phage as a culturable model system makes this thesis a major contribution to the understanding of environmental ssDNA phages.
Download or read book Genetic Engineering written by Jane Setlow and published by . This book was released on 1987-07-01 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single stranded RNA phages written by Paul Pumpens and published by CRC Press. This book was released on 2020-02-03 with total page 1832 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive guide to single-stranded RNA phages (family Leviviridae), first discovered in 1961. These phages played a unique role in early studies of molecular biology, the genetic code, translation, replication, suppression of mutations. Special attention is devoted to modern applications of the RNA phages and their products in nanotechnology, vaccinology, gene discovery, evolutionary and environmental studies. Included is an overview of the generation of novel vaccines, gene therapy vectors, drug delivery, and diagnostic tools exploring the role of RNA phage-derived products in the revolutionary progress of the protein tethering and bioimaging protocols. Key Features Presents the first full guide to single-stranded RNA phages Reviews the history of molecular biology summarizing the role RNA phages in the development of the life sciences Demonstrates how RNA phage-derived products have resulted in nanotechnological applications Presents an up-to-date account of the role played by RNA phages in evolutionary and environmental studies
Download or read book E coli Plasmid Vectors written by Nicola Casali and published by Humana Press. This book was released on 2010-11-10 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Download or read book Bacteriophages written by David R. Harper and published by Springer Nature. This book was released on 2021-01-30 with total page 1376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first major reference work dedicated to the mannifold industrial and medical applications of bacteriophages provides both theoretical and practical insights into the emerging field of bacteriophage biotechnology. The book introduces to bacteriophage biology, ecology and history and reviews the latest technologies and tools in bacteriophage detection, strain optimization and nanotechnology. Usage of bacteriophages in food safety, agriculture, and different therapeutic areas is discussed in detail. This book serves as essential guide for researchers in applied microbiology, biotechnology and medicine coming from both academia and industry.
Download or read book The Bacteriophages written by Richard Calendar and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been 10 years since Plenum included a series of reviews on bacte riophages, in Comprehensive Virology. Chapters in that series contained physical-genetic maps but very little DNA sequence information. Now the complete DNA sequence is known for some phages, and the se quences for others will soon follow. During the past 10 years two phages have come into common use as reagents: A phage for cloning single copies of genes, and Ml3 for cloning and DNA sequencing by the dideoxy termi nation method. Also during that period the use of alternative sigma fac tors by RNA polymerase has become established for SPOl and T4. This seems to be a widely used mechanism in bacteria, since it has been implicated in sporulation, heat shock response, and regulation of nitro gen metabolism. The control of transcription by the binding of A phage CII protein to the -35 region of the promoter is a recent finding, and it is not known how widespread this mechanism may be. This rapid progress made me eager to solicit a new series of reviews. These contributions are of two types. Each of the first type deals with an issue that is exemplified by many kinds of phages; chapters of this type should be useful in teaching advanced courses. Chapters of the second type provide comprehensive pictures of individual phage families and should provide valuable information for use in planning experiments.
Download or read book The Molecular Biology of Bacterial Virus Systems written by G. Hobom and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fact that none of the known DNA polymerases is able to initiate DNA chains but only to elongate from a free 3' -OH group raises the problem of how replication is initiated, both at the replication origin and on Okazaki frag ments. It was first shown by A. KORNBERG et al. that a general mechanism to initiate replication is through the formation of an RNA primer catalyzed by RNA polymerases or by a new class of enzymes, the primases (KORNBERG 1980). This mechanism, which can be used in the case of circular DNA molecules or linear DNAs that circularize or form concatemers, cannot be used at the ends of linear DNAs since the RNA primer is removed from the DNA chain, and there is no way of filling the gap resulting at the 5' -ends of the newly synthesized DNA chain. In some cases linear DNA molecules contain a palin dromic nucleotide sequence at the 3' -end that allows the formation of a hairpin structure which provides the needed free 3'-OH group for elongation. This mechanism, first proposed by CAVALIER-SMITH (1974) for eukaryotic DNA repli cation, was shown to take place in several systems (KORNBERG 1980, 1982). Another mechanism to initiate replication consists in the specific nicking of one of the strands of a circular double-stranded DNA, producing a 3'-OH group available for elongation (KORNBERG 1980).
Download or read book Serpin Structure and Evolution written by James Whisstock and published by Academic Press. This book was released on 2011-11-16 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems. This volume in the Methods in Enzymology series comprehensively covers the topic of serpins With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems
Download or read book Modern Microbial Genetics written by Uldis N. Streips and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: In accordance with its predecessor, the completely revised and expanded Second Edition of Modern Microbial Genetics focuses on how bacteria and bacteriophage arrange and rearrange their genetic material through mutation, evolution, and genetic exchange to take optimal advantage of their environment. The text is divided into three sections: DNA Metabolism, Genetic Response, and Genetic Exchange. The first addresses how DNA replicates, repairs itself, and recombines, as well as how it may be manipulated. The second section is devoted to how microorganisms interact with their environment, including chapters on sporulation and stress shock, and the final section contains the latest information on classic exchange mechanisms such as transformation and conjugation. Chapters include: * Gene Expression and Its Regulation * Single-Stranded DNA Phages * Genetic Tools for Dissecting Motility and Development of Myxococcus xanthus * Molecular Mechanism of Quorum Sensing * Transduction in Gram-Negative Bacteria * Genetic Approaches in Bacteria with No Natural Genetic Systems The editors also cultivate an attention to global regulatory systems throughout the book, elucidating how certain genes and operons in bacteria, defined as regulons, network and cooperate to suit the needs of the bacterial cell. With clear appreciation for the impact of molecular genomics, this completely revised and updated edition proves that Modern Microbial Genetics remains the benchmark text in its field.
Download or read book The Bacteriophages written by Richard Calendar and published by Oxford University Press. This book was released on 2006 with total page 761 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative, timely, and comprehensively referenced compendium on the bacteriophages explores current views of how viruses infect bacteria. In combination with classical phage molecular genetics, new structural, genomic, and single-molecule technologies have rendered an explosion in our knowledge of phages. Bacteriophages, the most abundant and genetically diverse type of organism in the biosphere, were discovered at the beginning of the 20th century and enjoyed decades of used as anti-bacterial agents before being eclipsed by the antibiotic era. Since 1988, phages have come back into the spotlight as major factors in pathogenesis, bacterial evolution, and ecology. This book reveals their compelling elegence of function and their almost inconceivable diversity.Much of the founding work in molecular biology and structural biology was done on bacteriophages. These are widely used in molecular biology research and in biotechnology, as probes and markers, and in the popular method of assesing gene expression.
Download or read book Bireplicon Filamentous Phages and the Production of Single stranded Plasmid DNA written by Jeffrey Vieira and published by . This book was released on 1988 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Virus Protein and Nucleoprotein Complexes written by J. Robin Harris and published by Springer. This book was released on 2018-06-13 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Subcellular Biochemistry series has recently embarked upon an almost encyclopaedic coverage of topics relating to the structure and function of macromolecular complexes (Volumes 82, 83 and 87). The present multi-author text covers numerous aspects of current research into molecular virology, with emphasis upon viral protein and nucleoprotein structure and function. Structural data from cryo-electron microscopy and X-ray crystallography is displayed throughout the book. The 17 chapters in the book cover diverse interesting topics, all currently under investigation, contributed by authors who are active actively involved in present-day research. Whilst structural aspects predominate, there is much consideration of the structure-function relationship. In addition, the book correlates with and extends from Volume 68 of the series “Structure and Physics of Viruses: An Integrated Textbook”. This book is directed primarily at professionals that work in the broad field of Structural Biology and will be of particular interest to Structural Virologists. The editors, David Bhella and Robin Harris, have much experience in virology and protein structure, respectively. Dr Bhella is Director of the Scottish Macromolecular Imaging Centre. Professor Robin Harris is the long-standing Series Editor of the Subcellular Biochemistry series. He has edited and contributed to several books in the series.
Download or read book Head Neck and Orofacial Infections E book written by James R. Hupp and published by Elsevier Health Sciences. This book was released on 2024-06-07 with total page 1306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing full-color coverage of best practices, Head, Neck, and Orofacial Infections: An Interdisciplinary Approach, 2nd Edition, is an authoritative resource offering in-depth guidelines to the diagnosis and management of pathology due to severe infections. Comprehensive, evidence-based coverage presents both cutting-edge and time-tested approaches to recognizing and handling infections. From well-known academia and clinical educator James Hupp and accomplished surgeon Elie Ferneini, with chapters authored by expert contributors, this book is ideal for use as a clinical resource for a wide array of healthcare providers, as well as to prepare for licensure examination and board certification. NEW! Cutting-edge content covers microbiologic nomenclature, anti-microbial agents, understanding of viruses and anti-viral drugs, the management of patients during pandemics, and the team approach to managing infections of unknown origin or resistant to the usual treatment strategies. NEW! Full-color clinical images enhance understanding of key concepts in the text. NEW! eBook version, included with print purchase, provides access to all the text, figures, and references with the ability to search, customize content, make notes and highlights, and have content read aloud. UPDATED! Appendices include illustrative case reports. Comprehensive, easy-to-read coverage addresses the basic science, clinical diagnosis, and holistic management of a broad range of head, neck, and orofacial infections with both time-tested and cutting-edge approaches to patient management. More than 500 photographs, radiographs, and illustrations demonstrate pathologies, procedures, and outcomes. World-class authors and contributors share their expertise from the disciplines including infectious disease, head and neck surgery, oral and maxillofacial surgery, plastic surgery, and otolaryngology, as well as other disciplines involving severe infections of the head, neck, and orofacial regions. State-of-the-art guidance reflects extensive experience with current techniques, as well as technological advances in managing head, neck, and orofacial infections. A logical, sectioned approach to the content includes three sections: I) issues that are common to all infections of the head and neck region, II) infections of specific parts of the region, and III) infections related to certain procedures, types of patients, unusual organisms, and medical-legal implications.
Download or read book Encyclopedia of Virology written by and published by Academic Press. This book was released on 2021-02-24 with total page 4109 pages. Available in PDF, EPUB and Kindle. Book excerpt: Encyclopedia of Virology, Fourth Edition, Five Volume Set builds on the solid foundation laid by the previous editions, expanding its reach with new and timely topics. In five volumes, the work provides comprehensive coverage of the whole virosphere, making this a unique resource. Content explores viruses present in the environment and the pathogenic viruses of humans, animals, plants and microorganisms. Key areas and concepts concerning virus classification, structure, epidemiology, pathogenesis, diagnosis, treatment and prevention are discussed, guiding the reader through chapters that are presented at an accessible level, and include further readings for those needing more specific information. More than ever now, with the Covid19 pandemic, we are seeing the huge impact viruses have on our life and society. This encyclopedia is a must-have resource for scientists and practitioners, and a great source of information for the wider public. Offers students and researchers a one-stop shop for information on virology not easily available elsewhere Fills a critical gap of information in a field that has seen significant progress in recent years Authored and edited by recognized experts in the field, with a range of different expertise, thus ensuring a high-quality standard
