Download or read book The Role of Tegument Proteins and the Dynamic Interplay They Have with One Another Throughout the Course of Herpes Simplex Virus Type 1 Infections written by Ekaette Francis Mbong and published by . This book was released on 2012 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus type 1 (HSV-1) is a neurotropic virus affecting up to 98% of the adult population. Central to this report is the proteinaceous layer termed the tegument found within the HSV-1 virion. The tegument is composed of>20 different viral proteins of varying stoichiometries. Tegument proteins are delivered to the host cell upon infection, providing this virus an advantage early in its replication cycle. Tegument proteins also function at late times in infection when they are synthesized at high levels. One of the most abundant tegument proteins is VP22, encoded by the U_L 49 gene, with over 2000 copies per virion. The studies described herein provided much insight into how VP22 promotes the HSV-1 replication cycle. We found the absence of VP22 during infection resulted in decreased mRNA levels at early times and decreased protein synthesis at late times. These results were distinct and separable with respect to both the genes affected and the timing during infection. Because U_L 49^- infections often result in secondary, compensatory mutations in the gene encoding vhs, we examined the effects of one such mutation as well as the role of vhs's RNase activity on protein synthesis in the presence and absence of VP22. Our results indicated secondary mutations that decreased vhs's RNase activity compensated for the lack of VP22 during HSV-1 infections. Further analyses indicated the absence of VP22 resulted in decreased translation efficiency independent of mRNA abundance, the result of cleavage of the 5' UTRs of viral mRNAs. In separate studies we focused on the tegument protein encoded by the U_L21 gene. Although much is known about the role of this protein at late times in infection, very little was known about its role at early times in HSV-1 infections. We found the absence of pUL21 resulted in decreased levels of several immediate early mRNAs, and their corresponding proteins, at early times in infection. Although HSV-1 establishes a persistent infection, and is therefore incurable, we have made several novel findings that contribute important knowledge to the herpesvirus field and will hopefully aid in the development of new therapeutics.

Download or read book Interactions Between the Tegument Proteins UL11 and UL16 and the Glycoprotein E of Herpes Simplex Virus written by Pei-Chun Yeh and published by . This book was released on 2009 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: The herpesvirus tegument region present between the virion envelope and the nucleocapsid contains more than 20 different virus-encoded proteins. The process of tegument assembly and final envelopment has been unfoldeding over the past few decades. It is thought that a few tegument proteins are added to the capsid in the nucleus, whereas most of them are acquired after entering the cytoplasm or traveling to the site of final envelopment at the trans-Golgi network. Overall, the research presented in this dissertation provides insights into the molecular mechanism of protein-protein interactions that may be involved in (or contribute to) assembly and maturation of herpes simplex virus type 1 (HSV-1). The UL11 (membrane-bound) and UL16 (capsid-associated) tegument proteins are conserved among all herpesviruses, and interaction between the two has been implicated in linking the viral capsid, tegument, and membrane during final envelopment process. Both in transfected and virus-infected cells, a subpopulation of the UL11 protein was found associated with detergent-resistant membranes via modifications with two fatty- acid chains (myristate and palmitate). UL11 can directly interact with UL16 in a manner dependent on the acidic cluster and leucine-isoleucine motifs of UL11. And, N-ethylmaleimide-modified UL16 was found to be incapable of binding UL11, suggesting that free cysteines in UL16 somehow play a role in the interaction. UL16 is stably associated with cytoplasmic capsids isolated from infected cells. In response to initial attachment of virus to the cell surface, an 'outside-in' signal is transmitted across the virion membrane, and as a result, UL16 is dissociated from the capsid. The mechanism by which the signal is sent to UL16 remains unclear but seems likely to be mediated by the glycoproteins on the virion envelope. A GST chimera bearing the cytoplasmic tail of glycoprotein E (gE. CT) was found to be capable of binding UL16 expressed in mammalian or insect cells. To better understand the molecular mechanism of this signaling process, the interaction network emanating from UL16 was investigated. In addition, previously using a GST pull-down assay, it was observed that UL16 interacts with virus-specific proteins from HSV- and PRV-infected cell lysates, providing evidence that UL16 is present in protein complexes. To characterize native complexes that contain UL16, a recombinant virus was constructed expressing a tagged derivative. Using the combination of tandem affinity purification and mass spectrometry analysis, we identified gE to be present in the complexes isolated from infected cells. The UL16-gE interaction was confirmed in co-immunoprecipitation assays with infected cell lysates. Moreover, mutational analyses of gE. CT have suggested that in infected cells UL16 may interact with gE. CT in both UL11-dependent and -independent manners. Based on all available data, we hypothesize that UL11, UL16, and gE may form a tripartite complex which plays a role in multiple aspects of the virus life cycle, including signaling events during virus attachment, virion maturation, or cell-to-cell spread. Collectively, our research focused on the protein-protein network has built a foundation for future studies, and also advanced our current knowledge of herpesvirus replication.

Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.

Download or read book Glycoprotein M and ESCRT in Herpes Simplex Virus Type 1 Assembly written by Yudan Ren and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus type 1 (HSV-1) has a large linear double-stranded DNA genome in an icosahedral capsid shell, a cell-derived lipid envelope and a proteinaceous tegument layer. There are over fifty viral proteins and many host proteins identified in HSV-1 virions. The final formation of mature virus particles requires the membrane wrapping of tegumented capsids in the cytoplasm, a process termed secondary envelopment. This process involves the coordination of numerous viral and cellular proteins and results in double-membrane structures with enveloped virions contained within cellular vesicles. Mature viruses are then released through the fusion of these virion-containing vesicles and plasma membranes. This thesis describes investigation into the functions of viral glycoprotein M (gM) and the cellular Endosomal Sorting Complexes Required for Transport (ESCRT) in secondary envelopment. Firstly, it has been reported that gH/L can be efficiently internalised and targeted to the TGN by the co-expression of gM in transfection assays. In order to examine the role of gM in guiding the localisation of viral proteins in infected cells, a HSV-1 gM deletion virus (∆gM), and its revertant virus were constructed. The major phenotype demonstrated was that the absence of gM caused the internalisation of cell surface gH/L to be inhibited and higher levels of gH/L to be observed on the cell surface. Further, lower levels of gH/L were detected in purified ∆gM virions, which was in agreement with the delayed entry kinetics, smaller plaque sizes and greater replication deficits at low multiplicity of infection observed in ∆gM infected cells. Over all the results presented in this thesis demonstrate that in infected cells the efficient incorporation of gH/L into virions relies on the function of gM in HSV-1. Secondly, during HSV-1 secondary envelopment the budding and scission of the viral envelope from the host membrane share topological similarities with the formation of intraluminal vesicle in multivesicular bodies, retrovirus budding, and abscission at the end of cytokinesis, processes that require the cellular ESCRT machinery. There are four multiprotein ESCRT complexes and many associated proteins involved in their regulation. It has been previously shown that the ESCRT-III complex and a functional ATPase VPS4 are required for HSV-1 secondary envelopment, but different from the strategy utilised by HIV-1, the recruitment of ESCRT during HSV-1 infection is independent of TSG101 and/or ALIX. Data presented in this thesis demonstrate that CHMP4A/B/C proteins of the ESCRT-III complex are specifically crucial for HSV-1 secondary envelopment. Simultaneous depletion of CHMP4A/B/C proteins significantly inhibited HSV-1 replication. Ultrastructure analysis revealed that there were virtually no extracellular virions in CHMP4A/B/C depleted samples while more free capsids were observed in the cytoplasm, although the nuclear capsids and primary envelopment events appeared to be normal. In order to identify interactions between HSV-1 and ESCRT proteins, 22 HSV-1 tegument proteins were cloned and tested against a panel of ESCRT and ESCRT-associated proteins in yeast two-hydrid assays. Analysis of positive hits from yeast two-hybrid interaction screens using GST pull-down, co-immunoprecipitation and protein co-localisation assays have validated interactions of pUL47 with CC2D1A/1B, CIN85, CHMP6 and ALIX, pUL46 and pUL49 with CC2D1A/1B and CIN85, and pUL16 with CC2D1A/1B. Furthermore, the newly identified ESCRT associated proteins CC2D1A and CC2D1B have been detected in purified virions. The role of the identified ESCRT proteins in HSV-1 replication has been investigated using siRNA depletion. Unfortunately siRNA depletions of the various ESCRT candidates individually or in combinations did not show any significant effect on HSV-1 replication. Overall these data suggest that unlike HIV and other retroviruses, HSV-1 has evolved multiple parallel pathways to hijack the ESCRT machinery to facilitate its replication, particularly, through the interactions that lead directly to the recruitment of CHMP4A/B/C proteins. Disruption of some of these pathways did not prevent HSV-1 replication in tissue culture, suggesting any one potential pathway is sufficient for ESCRT recruitment to sites of HSV-1 assembly.

Download or read book Herpes Simplex Virus Type 1 written by Kari Lynn Roberts and published by . This book was released on 2011 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus type 1 (HSV-1) replicates in the nucleus and buds through the nuclear membrane to access the cytosol and complete the maturation process. The HSV-1 protein pUL31, along with its binding partner, pUL34, has been previously shown to be required for nucleocapsids to successfully bud through the nuclear membrane. pUL31 is also required for efficient viral DNA synthesis and packaging. After the HSV-1 exits the nucleus, the capsid acquires several accessory proteins (tegument) and finally a mature envelope by budding into vesicles of the trans-Golgi network (TGN). These virion-laden vesicles traffic toward the cell periphery and through cortical actin until ultimately the virion is secreted upon fusion of the TGN vesicle with the plasma membrane. The data presented here reveals new roles for pUL31, as cells infected with a UL31-null virus were delayed for viral gene expression as well as activation of NF[kappa]B and c-Jun N-terminal kinase (JNK) in multiple cell lines. At least one representative from each kinetic class of viral genes was examined at various times post infection. The protein expression defects were not caused by a failure to enter cells, was not rescued by ICP27 expressed in trans and correlated with NF[kappa]B activation. The data shows that these defects were not observed in the absence of the pUL31 binding partner, pUL34, and that while most pUL31 is expressed at late times post infection, low levels are detectable as early as 2 hours post infection. Data presented in this work also demonstrates a role for the actin motor myosin Va in the secretion of HSV-1 virions. Expression of two isoforms of dominant negative myosin Va (DN-myoVa) decreased virion secretion by 5075% as well as significantly decreased surface expression of viral glycoproteins B, M and D. DN-myoVa colocalized with TGN markers and the conformation of native myosin Va in infected cells was altered by 4 hours post infection. These data suggest that myosin Va is involved in the egress of virion-laden TGN vesicles as they transit through cortical actin toward the plasma membrane.

Download or read book Proteasome dependent Entry of Herpes Simplex Virus written by Mark Delboy and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus entry into cells is a multistep process that engages the host cell machinery. The proteasome is a large, ATP-dependent, multisubunit protease that plays a critical role in the maintenance of cell homeostasis. A battery of assays were used to demonstrate that proteasome inhibitors blocked an early step in herpes simplex virus entry that occurred after capsid penetration into the cytosol but prior to capsid arrival at the nuclear periphery. Proteasome-dependent viral entry was not reliant on host or viral protein synthesis. MG132, a peptide aldehyde that competitively inhibits the degradative activity of the proteasome, had a reversible inhibitory effect on herpes simplex virus capsid transport. Herpes simplex virus can use endocytic or nonendocytic pathways to enter cells. These distinct entry routes were both dependent on proteasome-mediated proteolysis. In addition, herpes simplex virus successfully entered cells in the absence of a functional host ubiquitin-activating enzyme, suggesting that viral entry is ubiquitin independent. Herpes simplex virus immediate-early protein ICP0 is a multifunctional regulator of herpes simplex virus infection. Late in infection ICP0 interacts dynamically with cellular proteasomes. ICP0 has a RING finger domain with E3 ubiquitin ligase activity that is necessary for its IE functions. The fundamental and functional properties of ICP0 that is present in the virion tegument layer have not been well characterized. For these reasons, I sought to characterize tegument ICP0 and determine the role of tegument ICP0 during proteasome-dependent entry of herpes simplex virus. Protein compositions of wild-type and ICP0 null virions were similar, suggesting that the absence of ICP0 does not grossly impair virion assembly. Virions with mutations in the RING finger domain contained greatly reduced levels of tegument ICP0, suggesting that the domain influences the incorporation of ICP0. Virion ICP0 was resistant to removal by detergent and salt and was associated with capsids, features common to inner tegument proteins. ICP0 mutations that resulted in the absence of ICP0 in the tegument layer, allow herpes simplex virus to enter cells independently of the proteasome activity. I propose that proteasomal degradation of virion and/or host proteins is regulated by ICP0 to allow for efficient delivery of incoming herpes simplex virus capsids to the nucleus.

Download or read book Cell Biology of Herpes Viruses written by Klaus Osterrieder and published by Springer. This book was released on 2017-05-20 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes viruses are widely distributed in nature, causing disease in organisms as diverse as bivalves and primates, including humans. Each virus appears to have established a long-standing relationship with its host, and the viruses have the ability to manipulate and control the metabolism of host cells, as well as innate and adaptive antiviral immune responses. Herpes viruses maintain themselves within hosts in a latent state resulting in virus persistence for years – usually for the life span of the hosts. Herpes viruses comprise a large number of pathogens with diverse cellular targets and biological consequences of infection. What they have in common is their structure and the fact that they establish a dormant (latent) infection in their hosts that usually persists for life. The reviews here will highlight the general principles of herpes virus infection, with equal attention to overall principle and important difference. Also, the cell type- and life-style dependent differences in the establishment and maintenance of virus persistence will be covered.

Download or read book Inhibition of Pact Mediated Type 1 Interferon Production by Herpes Simplex Virus Type 1 Us11 Protein written by Chun Kew and published by . This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Inhibition of PACT Mediated Type 1 Interferon Production by Herpes Simplex Virus Type 1 Us11 Protein" by Chun, Kew, 喬駿, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Mammals have complicated antiviral innate immunity to combat viral infection and this poses a strong selection pressure on the viruses. As a result, many viruses have evolved different strategies to disrupt the function of hosts' antiviral innate immunity. Herpes simplex virus type 1 (HSV-1) is one of the examples. HSV-1 is a common and important human pathogen. HSV-1 infection induces type I interferons (IFNs) which restrict viral replication potently. To ensure persistent infection and successful replication, HSV-1 encodes several IFN-suppressing proteins. One example is Us11. Interaction between Us11 and various cellular proteins, such as PKR, RIG-I and PACT, were shown by other studies. However, exactly how Us11 suppresses IFN function remains to be elucidated. In this study, I discovered that Us11 specifically inhibits PACT induced activation of RIG-I. In HSV-1 infected cells, PACT and Us11 associate with each other tightly and this interaction prevents the interaction of PACT with RIG-I. It was also found that RNA binding domains on both PACT and Us11 are important for the association. In infection experiments, the increased production of IFN- during the infection of PACT-competent cells with Us11-deficient HSV-1 recombinant virus was not observed in infected PACT-compromised cells, suggesting the requirement of PACT for Us11 suppression of IFN production. To conclude, this study provides an explanation for Us11 antagonism of IFN production. My findings suggest that PACT is a novel target of HSV-1 IFN-antagonizing protein Us11. DOI: 10.5353/th_b5317061 Subjects: Interferon Herpes simplex virus

Download or read book Structure and Physics of Viruses written by Mauricio G. Mateu and published by Springer Science & Business Media. This book was released on 2013-06-04 with total page 734 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contemplates the structure, dynamics and physics of virus particles: From the moment they come into existence by self-assembly from viral components produced in the infected cell, through their extracellular stage, until they recognise and infect a new host cell and cease to exist by losing their physical integrity to start a new infectious cycle. (Bio)physical techniques used to study the structure of virus particles and components, and some applications of structure-based studies of viruses are also contemplated. This book is aimed first at M.Sc. students, Ph.D. students and postdoctoral researchers with a university degree in biology, chemistry, physics or related scientific disciplines who share an interest or are actually working on viruses. We have aimed also at providing an updated account of many important concepts, techniques, studies and applications in structural and physical virology for established scientists working on viruses, irrespective of their physical, chemical or biological background and their field of expertise. We have not attempted to provide a collection of for-experts-only reviews focused mainly on the latest research in specific topics; we have not generally assumed that the reader knows all of the jargon and all but the most recent and advanced results in each topic dealt with in this book. In short, we have attempted to write a book basic enough to be useful to M.Sc and Ph.D. students, as well as advanced and current enough to be useful to senior scientists with an interest in Structural and/or Physical Virology.

Download or read book Human Herpesviruses written by Yasushi Kawaguchi and published by Springer. This book was released on 2018-06-12 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces and reviews several topics for each human herpesvirus. One of the most important features of the book is that it covers aspects of both basic research and clinical medicine. Herpesviridae, a family of double-strand DNA viruses, has unique biological features by which these viruses establish latency after primary infection and reactivate in later life. Nine human herpesviruses are known so far, and each of them causes a variety of diseases in both primary infection and reactivation. Since the discovery of each human herpesvirus, an abundance of findings related to them has accumulated in basic research and clinical medicine. However, the vast majority of biological features is still masked in mystery. Furthermore, a strategy of treatment and prevention has not yet been established for most human herpesviruses. A wide range of readers will be interested in this volume with its treatment of problematic points and latest findings in the field.

Download or read book Scanning Electron Microscopy for the Life Sciences written by Heide Schatten and published by Cambridge University Press. This book was released on 2013 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Download or read book Epstein Barr Virus Volume 2 written by Christian Münz and published by Springer. This book was released on 2015-10-01 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epstein Barr virus (EBV) was discovered as the first human tumor virus around 50 years ago. Since its discovery in Burkitt’s lymphoma it has been associated with various other malignancies, infectious mononucleosis and even autoimmune diseases. The two book volumes on EBV summarize the first 50 years of research on this tumor virus, starting with historical perspectives on discovery, oncogenicity and immune control, reviewing the role that the virus plays in the various associated diseases and concluding with a discussion on how the immune system keeps persistent EBV infection under control in healthy EBV carriers and can be used to treat EBV associated diseases. The respective 32 chapters are written by international experts from three continents for health care providers, biomedical researchers and patients that are affected by EBV. The assembled knowledge should help to understand EBV associated diseases better and to develop EBV specific vaccination in the near future.

Download or read book Viral Molecular Machines written by Michael G. Rossmann and published by Springer Science & Business Media. This book was released on 2012-02-02 with total page 685 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will contain a series of solicited chapters that concern with the molecular machines required by viruses to perform various essential functions of virus life cycle. The first three chapters (Introduction, Molecular Machines and Virus Architecture) introduce the reader to the best known molecular machines and to the structure of viruses. The remainder of the book will examine in detail various stages of the viral life cycle. Beginning with the viral entry into a host cell, the book takes the reader through replication of the genome, synthesis and assembly of viral structural components, genome packaging and maturation into an infectious virion. Each chapter will describe the components of the respective machine in molecular or atomic detail, genetic and biochemical analyses, and mechanism. Topics are carefully selected so that the reader is exposed to systems where there is a substantial infusion of new knowledge in recent years, which greatly elevated the fundamental mechanistic understanding of the respective molecular machine. The authors will be encouraged to simplify the detailed knowledge to basic concepts, include provocative new ideas, as well as design colorful graphics, thus making the cutting-edge information accessible to broad audience.

Download or read book Molecular Virology of Human Pathogenic Viruses written by Wang-Shick Ryu and published by Academic Press. This book was released on 2016-03-30 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author's more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. - Presents viruses within their family structure - Contains recommended journal articles with perspectives to put primary literature in context - Includes integrated recommended reading references within each chapter - Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor's manual, study guide, and test bank

Download or read book Crop ecology cultivation and uses of cactus pear written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2018-06-05 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cactus plants are precious natural resources that provide nutritious food for people and livestock, especially in dryland areas. Originally published in 1995, this extensively revised edition provides fresh insights into the cactus plant’s genetic resources, physiological traits, soil preferences and vulnerability to pests. It provides invaluable guidance on managing the resource to support food security and offers tips on how to exploit the plant’s culinary qualities.

Download or read book A Guide to Human Gene Therapy written by Roland W. Herzog and published by World Scientific. This book was released on 2010 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. Non-viral gene therapy / Sean M. Sullivan -- 2. Adenoviral vectors / Stuart A. Nicklin and Andrew H. Baker -- 3. Retroviral vectors and integration analysis / Cynthia C. Bartholomae [und weitere] -- 4. Lentiviral vectors / Janka Matrai, Marinee K.L. Chuah and Thierry VandenDriessche -- 5. Herpes simplex virus vectors / William F. Goins [und weitere] -- 6. Adeno-Associated Viral (AAV) vectors / Nicholas Muzyczka -- 7. Regulatory RNA in gene therapy / Alfred. S. Lewin -- 8. DNA integrating vectors (Transposon, Integrase) / Lauren E. Woodard and Michele P. Calos -- 9. Homologous recombination and targeted gene modification for gene therapy / Matthew Porteus -- 10. Gene switches for pre-clinical studies in gene therapy / Caroline Le Guiner [und weitere] -- 11. Gene therapy for central nervous system disorders / Deborah Young and Patricia A. Lawlor -- 12. Gene therapy of hemoglobinopathies / Angela E. Rivers and Arun Srivastava -- 13. Gene therapy for primary immunodeficiencies / Aisha Sauer, Barbara Cassani and Alessandro Aiuti -- 14. Gene therapy for hemophilia / David Markusic, Babak Moghimi and Roland Herzog -- 15. Gene therapy for obesity and diabetes / Sergei Zolotukhin and Clive H. Wasserfall -- 16. Gene therapy for Duchenne muscular dystrophy / Takashi Okada and Shin'ichi Takeda -- 17. Cancer gene therapy / Kirsten A.K. Weigel-Van Aken -- 18. Gene therapy for autoimmune disorders / Daniel F. Gaddy, Melanie A. Ruffner and Paul D. Robbins -- 19. Gene therapy for inherited metabolic storage diseases / Cathryn Mah -- 20. Retinal diseases / Shannon E. Boye, Sanford L. Boye and William W. Hauswirth -- 21. A brief guide to gene therapy treatments for pulmonary diseases / Ashley T. Martino, Christian Mueller and Terence R. Flotte -- 22. Cardiovascular disease / Darin J. Falk, Cathryn S. Mah and Barry J. Byrne

Download or read book Cell Stress Proteins written by Stuart K. Calderwood and published by Springer Science & Business Media. This book was released on 2009-02-13 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book surveys the current knowledge concerning the expression and function of stress proteins in different organisms, ranging from prokaryotes to humans. It provides an overview of the diversity and complex evolutionary history of cell stress proteins and describes their function and expression in different eukaryote models. The book will appeal to researchers and scientists in biochemistry, cell biology, microbiology, immunology, and genetics.