Download or read book The Role of Environmental Microbiota in the Persistence of Listeria Monocytogenes in Food Processing Facilities written by Maria Laura Rolon and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food safety is of paramount importance for production of wholesome and nutritious food. Throughout my dissertation, I present results of four studies exploring the relationship between Listeria monocytogenes, a deadly foodborne pathogen, and environmental microbiota found in food processing facilities. L. monocytogenes can inhabit a wide range of habitats (e.g., soil, water, animals, insects) and its ability to adapt to different environmental conditions (e.g., low temperatures, high salt concentrations, low pH) allows for its survival in food processing environments, which can result in the potential for recurring contamination of food. To control the presence of L. monocytogenes, food processors rely on cleaning and sanitizing operations coupled with Environmental Monitoring Programs. While L. monocytogenes is typically susceptible to the cleaning and sanitizing protocols applied in food processing facilities, it may survive the action of sanitizers by forming, or residing inside biofilms. However, in food processing environments, L. monocytogenes resides with other environmental microorganisms that are introduced to the facilities with raw products or personnel. The presence of other microorganisms can facilitate formation of robust multi-species biofilms that may enhance the survival and persistence of L. monocytogenes. Currently, limited information is available regarding the potential associations between environmental microbiota and the presence of foodborne pathogens in food processing facilities. My dissertation research aimed to address some of the critical gaps in the understanding of the role of environmental microbiota in the survival and persistence of L. monocytogenes in food processing environments. iv To begin unraveling the role of environmental microbiota in the persistence of L. monocytogenes in food processing environments, I first sought to characterize the microbiota of three tree fruit packing facilities and statistically assess whether certain taxa co-occur with L. monocytogenes (Chapter 2). I observed a recurring presence of L. monocytogenes and a distinct microbiota composition in the monitored facilities throughout two seasons. Importantly, I found that bacterial taxa from taxonomic families Pseudomonadaceae, Xanthomonadaceae, and Microbacteriaceae were present in a significantly higher relative abundance in L. monocytogenes-positive samples, especially in the facility with a persistent L. monocytogenes contamination. While some members of these bacterial families have been studied as food spoilage organisms (e.g., Pseudomonas spp.), emerging human pathogens (e.g., Stenotrophomonas spp.), or plant pathogens (e.g., Xanthomonas spp.), there is limited information available on potential interactions between these species and L. monocytogenes. Furthermore, their role in the survival and persistence of L. monocytogenes in food processing facilities is severely understudied. In Chapter 3, I seek to understand how the detected bacterial taxa interact with L. monocytogenes. Given that the survival of L. monocytogenes under sanitizer pressure may be facilitated by biofilms formed in food processing environment, I aimed to assess the effect of biofilms formed by environmental microbiota on the survival during exposure to a commonly used sanitizer, benzalkonium chloride. I isolated environmental microbiota from families Pseudomonadaceae, Xanthomonadaceae, Microbacteriaceae, and Flavobacteriaceae from tree fruit packing environments, and tested their ability to form biofilms in single- and multi-family assemblages with L. monocytogenes. I found that microbial assemblages of v increasing complexity (i.e., increasing number of families) generally formed more biofilm and contained a greater concentration of L. monocytogenes, compared to monoculture biofilms. I further tested the effects of multi-species biofilm formation on the sanitizer tolerance of L. monocytogenes, by exposing multi-species biofilms to sanitizers and quantifying the die-off kinetics of L. monocytogenes throughout a 2-hour period. In Chapter 4, I aimed to assist fruit packers by providing practical tools to enhance the control of L. monocytogenes through traditional chemical-based cleaning and sanitizing. I tested four cleaning and sanitizing cleaning and sanitizing protocols in the fruit packing environments to determine their effectiveness in controlling L. monocytogenes and examined their effect on environmental microbiota. I found that the cleaning and sanitizing protocol that included a disinfectant with a biofilm-degrading ability was most effective in reducing the frequency of detected L. monocytogenes in the sampled areas. While the total microbial load generally decreased after the application of cleaning and sanitizing protocols, the microbiota composition did not appear to be significantly affected by the treatments. In the last research chapter (Chapter 5), I tested the ability of two lactic acid bacteria strains to inhibit L. monocytogenes in a monoculture as well as in the context of environmental microbiota from food processing facilities. Microbial strains with antilisterial activity have been previously assessed as alternative strategy to control L. monocytogenes in food processing environments, however, their performance in the context of environmental microbiota from ice cream processing facilities has not been tested before. Here, I collected environmental microbiota from three small-scale ice cream processing facilities and tested whether two lactic acid vi bacteria could attach and effectively inhibit L. monocytogenes when co-cultured with the collected environmental microbiota. I observed that the microbiota composition of ice cream processing facilities may affect the antilisterial ability of two lactic acid bacteria strains and their attachment to surfaces. In particular, the presence of Pseudomonas significantly reduced the antilisterial ability of the tested strains. The knowledge generated through my studies on the role of food processing facilities' microbiota will aid in designing tailored cleaning, sanitizing, and/or biological control protocols for control of L. monocytogenes. For example, cleaning and sanitizing could be redesigned to control taxa that facilitate Listeria's persistence, or new cleaning chemistries can be developed to improve biofilm control in food processing facilities.

Download or read book Listeria monocytogenes in the Food Processing Environment written by Kieran Jordan and published by Springer. This book was released on 2015-04-22 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Brief focuses on Listeria monocytogenes, from isolation methods and characterization (including whole genome sequencing), to manipulation and control. Listeriosis, a foodborne disease caused by Listeria monocytogenes is a major concern for public health authorities. In addition, addressing issues relating to L. monocytogenes is a major economic burden on industry. Awareness of its ubiquitous nature and understanding its physiology and survival are important aspects of its control in the food processing environment and the reduction of the public health concern.

Download or read book Foodborne Bacterial Pathogens written by Michael Doyle and published by CRC Press. This book was released on 1989-02-24 with total page 818 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria are estimated to cause some 24 million cases of diarrheal disease annually in the US. These papers have wide importance providing background information and recent research findings and giving a comprehensive, current understanding of bacterial pathogens associated with foods and their role

Download or read book Risk Assessment of Listeria Monocytogenes in Ready to eat Foods written by World Health Organization and published by . This book was released on 2004 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cases of listeriosis appear to be predominantly associated with ready-to-eat products. FAO and WHO have undertaken a risk assessment of Listeria monocytogenes in ready-to-eat foods, prepared and reviewed by an international team of scientists. Input was received from several international fora including expert consultations and Codex Alimentarius committee meetings as well as via public and peer review. This interpretative summary provides an overview of how the risk assessment was undertaken and the results. In particular, it provides information relevant to risk managers addressing problems posed by this pathogen in ready-to-eat foods. It includes answers to the specific risk management questions posed by the Codex Committee on Food Hygiene and outlines the issues to be considered when implementing control measures, including the establishment of microbiological criteria.

Download or read book The Bad Bug Book written by FDA and published by Imp. This book was released on 2004 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Bad Bug was created from the materials assembled at the FDA website of the same name. This handbook provides basic facts regarding foodborne pathogenic microorganisms and natural toxins. It brings together in one place information from the Food & Drug Administration, the Centers for Disease Control & Prevention, the USDA Food Safety Inspection Service, and the National Institutes of Health.

Download or read book Handbook of Hygiene Control in the Food Industry written by John Holah and published by Elsevier. This book was released on 2005-10-30 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developments such as the demand for minimally-processed foods have placed a renewed emphasis on good hygienic practices in the food industry. As a result there has been a wealth of new research in this area. Complementing Woodhead’s best-selling Hygiene in the food industry, which reviews current best practice in hygienic design and operation, Handbook of hygiene control in the food industry provides a comprehensive summary of the key trends and issues in food hygiene research. Developments go fast: results of the R&D meanwhile have been applied or are being implemented as this book goes to print.Part one reviews research on the range of contamination risks faced by food processors. Building on this foundation, Part two discusses current trends in the design both of buildings and types of food processing equipment, from heating and packaging equipment to valves, pipes and sensors. Key issues in effective hygiene management are then covered in part three, from risk analysis, good manufacturing practice and standard operating procedures (SOPs) to improving cleaning and decontamination techniques. The final part of the book reviews developments in ways of monitoring the effectiveness of hygiene operations, from testing surface cleanability to sampling techniques and hygiene auditing.Like Hygiene in the food industry, this book is a standard reference for the food industry in ensuring the highest standards of hygiene in food production. Standard reference on high hygiene standards for the food industry Provides a comprehensive summary of the key trends in food hygiene research Effective hygiene management strategies are explored

Download or read book Microbial Biofilms written by Hilary M. Lappin-Scott and published by Cambridge University Press. This book was released on 2003-09-18 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry and ecology of biofilms from industrial, medical and other viewpoints.

Download or read book Principles of Microbiological Troubleshooting in the Industrial Food Processing Environment written by Jeffrey Kornacki and published by Springer Science & Business Media. This book was released on 2010-05-19 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Microbiological Troubleshooting in the Industrial Food Processing Environment provides proven approaches and suggestions for finding sources of microbiological contamination of industrially produced products. Industrial food safety professionals find themselves responsible for locating and eliminating the source(s) of food contamination. These are often complex situations for which they have not been adequately prepared. This book is written with them, the in-plant food safety/quality assurance professional, in mind. However, other professionals will also benefit including plant managers, regulatory field investigators, technical food safety policy makers, college instructors, and students of food science and microbiology. A survey of the personal and societal costs of microbial contamination of food is followed by a wide range of respected authors who describe selected bacterial pathogens, emerging pathogens, spoilage organisms and their significance to the industry and consumer. Dr. Kornacki then provides real life examples of in-plant risk areas / practices (depicted with photographs taken from a wide variety of food processing facilities). Factors influencing microbial growth, survival and death area also described. The reader will find herein a practical framework for troubleshooting and for assessing the potential for product contamination in their own facilities, as well as suggestions for conducting their own in-plant investigations. Selected tools for testing the environment and statistical approaches to testing ingredients and finished product are also described. The book provides suggestions for starting up after a processing line (or lines) have been shut down due to a contamination risk. The authors conclude with an overview of molecular subtyping and its value with regard to in-plant investigations. Numerous nationally recognized authors in the field have contributed to the book. The editor, Dr. Jeffery L. Kornacki, is President and Senior Technical Director of the consulting firm, Kornacki Microbiology Solutions in Madison, Wisconsin. He is also Adjunct Faculty with the Department of Food Science at the University of Georgia and also with the National Food Safety & Toxicology Center at Michigan State University.

Download or read book The Microbiological Safety of Low Water Activity Foods and Spices written by Joshua B. Gurtler and published by Springer. This book was released on 2014-12-08 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: Low water activity (aw) and dried foods such as dried dairy and meat products, grain-based and dried ready-to-eat cereal products, powdered infant formula, peanut and nut pastes, as well as flours and meals have increasingly been associated with product recalls and foodborne outbreaks due to contamination by pathogens such as Salmonella spp. and enterohemorrhagic E. coli. In particular, recent foodborne outbreaks and product recalls related to Salmonella-contaminated spices have raised the level of public health concern for spices as agents of foodborne illnesses. Presently, most spices are grown outside the U.S., mainly in 8 countries: India, Indonesia, China, Brazil, Peru, Madagascar, Mexico and Vietnam. Many of these countries are under-developed and spices are harvested and stored with little heed to sanitation. The FDA has regulatory oversight of spices in the United States; however, the agency’s control is largely limited to enforcing regulatory compliance through sampling and testing only after imported foodstuffs have crossed the U.S. border. Unfortunately, statistical sampling plans are inefficient tools for ensuring total food safety. As a result, the development and use of decontamination treatments is key. This book provides an understanding of the microbial challenges to the safety of low aw foods, and a historic backdrop to the paradigm shift now highlighting low aw foods as vehicles for foodborne pathogens. Up-to-date facts and figures of foodborne illness outbreaks and product recalls are included. Special attention is given to the uncanny ability of Salmonella to persist under dry conditions in food processing plants and foods. A section is dedicated specifically to processing plant investigations, providing practical approaches to determining sources of persistent bacterial strains in the industrial food processing environment. Readers are guided through dry cleaning, wet cleaning and alternatives to processing plant hygiene and sanitation. Separate chapters are devoted to low aw food commodities of interest including spices, dried dairy-based products, low aw meat products, dried ready-to-eat cereal products, powdered infant formula, nuts and nut pastes, flours and meals, chocolate and confectionary, dried teas and herbs, and pet foods. The book provides regulatory testing guidelines and recommendations as well as guidance through methodological and sampling challenges to testing spices and low aw foods for the presence of foodborne pathogens. Chapters also address decontamination processes for low aw foods, including heat, steam, irradiation, microwave, and alternative energy-based treatments.

Download or read book Climate change Unpacking the burden on food safety written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2020-03-01 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Climate change is causing unprecedented damage to our ecosystem. Increasing temperatures, ocean warming and acidification, severe droughts, wildfires, altered precipitation patterns, melting glaciers, rising sea levels and amplification of extreme weather events have direct implications for our food systems. While the impacts of such environmental factors on food security are well known, the effects on food safety receive less attention. The purpose of Climate change: Unpacking the burden on food safety is to identify and attempt to quantify some current and anticipated food safety issues that are associated with climate change. The food safety hazards considered in the publication are foodborne pathogens and parasites, harmful algal blooms, pesticides, mycotoxins and heavy metals with emphasis on methylmercury. There is also, a dedicated section on the benefits of forward-looking approaches such as horizon scanning and foresight, which will not only aid in anticipating future challenges in a shifting global food safety landscape, but also help build resilient food systems that can be continually updated as more knowledge is assimilated. By building a more widespread and better understanding of the consequences climate change has on food safety, it is hoped that this document will aid in fostering stronger international cooperation in making our food safer by reducing the global burden of these concerns.

Download or read book Foodborne Pathogens written by Joshua B. Gurtler and published by Springer. This book was released on 2017-06-14 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foodborne illnesses continue to be a major public health concern. All members of a particular bacterial genera (e.g., Salmonella, Campylobacter) or species (e.g., Listeria monocytogenes, Cronobacter sakazakii) are often treated by public health and regulatory agencies as being equally pathogenic; however, this is not necessarily true and is an overly conservative approach to ensuring the safety of foods. Even within species, virulence factors vary to the point that some isolates may be highly virulent, whereas others may rarely, if ever, cause disease in humans. Hence, many food safety scientists have concluded that a more appropriate characterization of bacterial isolates for public health purposes could be by virotyping, i.e., typing food-associated bacteria on the basis of their virulence factors. The book is divided into two sections. Section I, “Foodborne Pathogens and Virulence Factors,” hones in on specific virulence factors of foodborne pathogens and the role they play in regulatory requirements, recalls, and foodborne illness. The oft-held paradigm that all pathogenic strains are equally virulent is untrue. Thus, we will examine variability in virulence between strains such as Listeria, Salmonella, Campylobacter, Cronobacter, etc. This section also examines known factors capable of inducing greater virulence in foodborne pathogens. Section II, “Foodborne Pathogens, Host Susceptibility, and Infectious Dose” , covers the ability of a pathogen to invade a human host based on numerous extraneous factors relative to the host and the environment. Some of these factors include host age, immune status, genetic makeup, infectious dose, food composition and probiotics. Readers of this book will come away with a better understanding of foodborne bacterial pathogen virulence factors and pathogenicity, and host factors that predict the severity of disease in humans.

Download or read book Microbial Stress From Model Organisms to Applications in Food Microbiotechnology and Medicine written by Aleksandra P. Djukic-Vukovic and published by Frontiers Media SA. This book was released on 2022-07-27 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Interspecies Interactions Effects On Virulence And Antimicrobial Susceptibility Of Bacterial And Fungal Pathogens written by Giuseppantonio Maisetta and published by Frontiers Media SA. This book was released on 2020-10-29 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Download or read book Quantitative Microbiology in Food Processing written by Anderson de Souza Sant'Ana and published by John Wiley & Sons. This book was released on 2017-02-06 with total page 611 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microorganisms are essential for the production of many foods, including cheese, yoghurt, and bread, but they can also cause spoilage and diseases. Quantitative Microbiology of Food Processing: Modeling the Microbial Ecology explores the effects of food processing techniques on these microorganisms, the microbial ecology of food, and the surrounding issues concerning contemporary food safety and stability. Whilst literature has been written on these separate topics, this book seamlessly integrates all these concepts in a unique and comprehensive guide. Each chapter includes background information regarding a specific unit operation, discussion of quantitative aspects, and examples of food processes in which the unit operation plays a major role in microbial safety. This is the perfect text for those seeking to understand the quantitative effects of unit operations and beyond on the fate of foodborne microorganisms in different foods. Quantitative Microbiology of Food Processing is an invaluable resource for students, scientists, and professionals of both food engineering and food microbiology.

Download or read book Microbiological Testing in Food Safety Management written by International Commission on Microbiological Specifications for Foods and published by Springer Science & Business Media. This book was released on 2002 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest book in this excellent series describes the role of microbiological testing in modern food safety management systems. It explores how risk assessment and risk management can be used to establish goals for use in controlling food borne illness, and provides guidelines for establishing effective management systems to control specific hazards in foods. This groundbreaking book will interest food microbiologists, researchers, and others in the food industry, regulatory agencies and academia worldwide.

Download or read book Bacterial Biofilms written by Sadik Dincer and published by BoD – Books on Demand. This book was released on 2020-10-07 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines biofilms in nature. Organized into four parts, this book addresses biofilms in wastewater treatment, inhibition of biofilm formation, biofilms and infection, and ecology of biofilms. It is designed for clinicians, researchers, and industry professionals in the fields of microbiology, biotechnology, ecology, and medicine as well as graduate and postgraduate students.

Download or read book Molecular Food Microbiology written by Dongyou Liu and published by CRC Press. This book was released on 2021-04-11 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: The elucidation of DNA double helix in 1953 and the publication of DNA cloning protocol in 1973 have put wings under the sail of molecular biology, which has since quietly revolutionized many fields of biological science, including food microbiology. Exploiting the power and versatility of molecular technologies, molecular food microbiology extends and greatly improves on phenotypically based food microbiology, leading to the development of better diagnostics for foodborne infections and intoxications, and contributing to the design of more effective therapeutics and prophylaxes against foodborne diseases. Forming part of the Food Microbiology series, Molecular Food Microbiology provides a state of art coverage on molecular techniques applicable to food microbiology. While the introductory chapter contains an overview on the principles of current DNA, RNA and protein techniques and discusses their utility in helping solve practical problems that food microbiology is facing now and in the future, the remaining chapters present detailed moleuclar analyses of selective foodborne viruses, bacteria, fungi and parasites. Key Features: Contains a state of art overview on moleuclar techniques applicable to food microbiology research and development Presents in-depth molecular analysis of selective foodborne viruses, bacteria, fungi and parasites Highlights the utility of molecular tecniques for accurate diagnosis and effective control of foodborne diseases Includes expert contributions from international scientists involved in molecular food microbiology research Represents a highly informative textbook for students majoring in food, medical, and veterinary microbiology Offers a contemporary reference for scholars and educators wishing to keep abreast with the latest developments in molecular food microbiology With contributions from international scientists involved in molecular food microbiology research, this book constitutes an informative textbook for undergraduates and postgraduates majoring in food, medical, and veterinary microbiology; represents an indispensable guide for food, medical, and veterinary scientists engaged in molecular food microbiology research and development; and offers a contemporary update for scholars and educators trying to keep in touch with the latest developments in molecular food microbiology.