Download or read book The Regulation of the General Stress Response in the Yeast Saccharomyces Cerevisiae written by Sohini Bose and published by . This book was released on 2007 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Yeast Stress Responses written by Stefan Hohmann and published by Springer Science & Business Media. This book was released on 2007-10-23 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.

Download or read book Biology of Microorganisms on Grapes in Must and in Wine written by Helmut König and published by Springer. This book was released on 2017-11-01 with total page 711 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of the book begins with the description of the diversity of wine-related microorganisms, followed by an outline of their primary and energy metabolism. Subsequently, important aspects of the secondary metabolism are dealt with, since these activities have an impact on wine quality and off-flavour formation. Then chapters about stimulating and inhibitory growth factors follow. This knowledge is helpful for the growth management of different microbial species. The next chapters focus on the application of the consolidated findings of molecular biology and regulation the functioning of regulatory cellular networks, leading to a better understanding of the phenotypic behaviour of the microbes in general and especially of the starter cultures as well as of stimulatory and inhibitory cell-cell interactions during wine making. In the last part of the book, a compilation of modern methods complete the understanding of microbial processes during the conversion of must to wine.This broad range of topics about the biology of the microbes involved in the vinification process could be provided in one book only because of the input of many experts from different wine-growing countries.

Download or read book Mechanism and Regulation of the Stress Response in Saccharomyces Cerevisiae written by Eric Edward Powers and published by . This book was released on 1998 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Oxidative Stress Response in the Yeast Saccharomyces Cerevisiae and Its Control by YAP1 and SKN7 written by Jaekwon Lee and published by . This book was released on 1998 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Molecular Biology of Schizosaccharomyces pombe written by Richard Egel and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.

Download or read book Regulation of the Stress response Protein Kinase Snf1 in Saccharomyces Cerevisiae written by LaKisha Barrett and published by . This book was released on 2011 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Gene Regulation During Stress Response Transcription in Saccharomyces Cerevisiae written by Christian Miller and published by . This book was released on 2013 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Regulation of the Cell Division Cycle of Saccharomyces Cerevisiae by the Oxidative Stress Response written by Peter O'Callaghan and published by . This book was released on 2005 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dynamic Signaling and Adaptation in Saccharomyces Cerevisiae Stress Response Pathways written by Zohreh AkhavanAghdam and published by . This book was released on 2017 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Living cells respond to environmental cues through complex signaling and gene regulatory networks. A common theme throughout this thesis will be exploring design principles in biological networks and how they operate dynamically to process information and make decisions. In Chapter 1, we tackle how different types of stresses induce distinct nuclear translocation dynamics of Msn2, an outstanding question in the field. In the absence of stress, PKA phosphorylates Msn2, causing it to be exported out of the nucleus. In response to stress, PKA activity is inhibited, Msn2 is dephosphorylated and translocated into the nucleus. In response to glucose limitation, Msn2 exhibits an initial homogenous pulse of nuclear translocation followed by sporadic nuclear pulses with dose-dependent frequency, but in response to osmotic stress Msn2 undergoes a single translocation pulse with dose-dependent duration. We hypothesized that the difference between glucose limitation and osmotic stress-induced Msn2 dynamics might be a result of glucose limitation-dependent Snf1 activation, since previous studies suggest that Snf1 and PKA mutually inhibit each other. We use modeling and experiments to demonstrate that these different upstream network structures could, in fact, be responsible for the differences we see in Msn2 translocation dynamics. In Chapter 2, we study a recurring scheme in gene regulatory networks, which is combinatorial gene regulation by seemingly redundant transcription factors (TFs), using time-lapse microscopy and microfluidics. We use the seemingly redundant yeast homologous stress responsive TFs Msn2 and Msn4 as a model to quantitatively study the functional relevance of closely related TFs in the same single cells and find that Msn2 and Msn4 have non-redundant and distinct functions in combinatorial gene regulation. In response to a transient input, either Msn2 or Msn4 alone is sufficient to induce the expression of target genes with fast kinetics promoters. Target genes with slow kinetics promoters, however, require activation of both Msn2 and Msn4 in these conditions. Importantly, slow kinetic promoter activation is dependent on duration of the upstream signal because in response to a prolonged input, slow kinetic promoter activation no longer requires both Msn2 and Msn4. Thus, in Chapter 2, we determine that coordinated gene regulation by seemingly redundant TFs is not fixed, but rather dependent on the dynamics of upstream signals. In Chapter 3, we demonstrate that cells retain a memory of many of upstream signaling events that occur in response to stress, which primes the cells to respond to future severe stress events. We use microfluidics and time-lapse microscopy to modulate the amplitude and duration of priming stimulus and also increase the break time in between the priming stimulus and severe stress. Using this system, we have determined that cells acquire an amplitude-dependent short-term memory of priming stimulus, which is induced and lost rapidly, and a duration-dependent long-term memory which is stable for a long period of time before finally declining after 100 minutes. We use this information about the dynamical specificity of different types of cellular memory and their stability to determine the cellular pathways responsible for the observed memory.

Download or read book Regulation of Stress Induced Gene Expression in Saccharomyces Cerevisiae written by Vasudha Bharatula and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription initiation is complex process involving transcription factors(TF), co-activators, nucleosome remodelers and the pre-initiation complex (PIC);general transcription factors (GTF) and RNA polymerase II (RNAP2). The spatiotemporalorganization of these different proteins and their role in regulatingplasticity and selectivity of transcriptional reprogramming in various stressesremains unknown. I describe the role of Multicopy Suppressor of Snf1 mutation(Msn2); a stress responsive transcription factor, Mediator (coactivator) andnucleosome remodeling in fine-tuning gene expression in response to stress inyeast.Msn2 exhibits distinct patterns of nucleo-cytoplasmic oscillations indifferent stresses. I determined that Msn2 target genes exhibited fast or slowinduction kinetics in response to transient and persistent Msn2 nuclearoccupancy respectively. Chromatin immunoprecipitation studies revealed thatMsn2 binds promoters of common stress genes as well as condition specificgenes in nutrient and oxidative stresses, suggesting that TF dynamics could playa role in selective gene regulation in different stresses. Additionally, Msn2binding to ~30 oxidative genes was dependent on Yap1. However, deleting Msn2did not lead to a significant decrease in expression of oxidative stress responsegenes as did the absence of Yap1. These results suggest that indirectcooperativity between Msn2 and Yap1 could lead to selective promoter bindingbut the effects of such interactions on gene expression remain unclear.I studied the role of Mediator in native conditions, where its function has not been adequately addressed. Mediator occupies chromosomal interacting domains (CID), which mark boundaries between interacting genomic regions, suggesting that Mediator could play a significant role in higher-order genome organization along with functioning as a coactivator.Finally, I assessed the combined effect of Msn2, Mediator and nucleosome remodeling on gene expression upon nutrient deprivation. Both Msn2 and Mediator are selectively recruited to promoters of genes activated and repressed in nutrient stress. Genes activated by Msn2 exhibited rapid loss of nucleosomes, in an Msn2 dependent manner. In contrast, repression of genes was not accompanied by a significant gain in nucleosomes, suggesting additional mechanisms of repression could exist. The absence of Msn2, significantly decreased Mediator recruitment at promoters of target genes, indicating that Mediator- TF interactions are crucial for gene expression changes in stress. Interestingly, both RNAP2 and Mediator remained poised at promoters of repressed genes, possibly to re-initiate transcription once the stress has passed.These findings offer novel insights on how Msn2 regulates expression of specific genes depending on the environment by modulating its dynamics and genomic binding. The binding of Msn2 helps recruit Mediator and facilitate nucleosome loss at genes induced in stress. Whereas, repressed genes seems to possess Mediator and RNAP2 in an inactive form which could be the mechanism of repression and a way to activate these genes immediate after stress.

Download or read book Analysis of Regulatory Mechanisms Controlling Secretory and Stress Response Pathways in Saccharomyces Cerevisiae written by Yvonne Vahlensieck and published by . This book was released on 1995 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterizing the Role of Gds1p in the Saccharomyces Cerevisiae Environmental Stress Response written by Mihai-Vlad Cotrut and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The transcription factors Msn2p and Msn4p are major components of the Saccharomyces cerevisiae environmental stress response. Their transient activation/deactivation is dependent on a regulatory network centering on nucleocytoplasmic shuttling but also includes a range of other mechanisms. The acetyltransferase complex, NuA4 has been implicated in repression of Msn2p yet the mechanism is largely unknown. Gds1p is an uncharacterized yeast protein identified in a recent study as a physical interactor and acetylation target of NuA4. Gds1 protein level is dependant both on NuA4 and environmental stress and our analysis shows it to be involved in the nuclear exclusion of Msn2p in the absence of stress. Unstressed cells lacking GDS1 exhibit increased nuclear accumulation Msn2p and an increase in transcription of the stress reporter gene, HSP12. My work supports a model in which Gds1 and NuA4 can work independently to inhibit the Msn2/4 dependant yeast stress response in the absence of stress.

Download or read book Characterization of the Oxidative Stress Response in the Yeast Saccharomyces Cerevisiae written by Lindsay Philip Collinson and published by . This book was released on 1995 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book In VIVO Footprinting written by I.L. Cartwright and published by Elsevier Science. This book was released on 1997-08-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The revolution in biological research initiated by the demonstration that particular DNA molecules could be isolated, recombined in novel ways, and conveniently replicated to high copy number in vivo for further study, that is, the recombinant DNA era, has spawned many additional advances, both methodological and intellectual, that have enhanced our understanding of cellular processes to an astonishing degree. As part of the subsequent outpouring of information, research exploring the mechanisms of gene regulation, both in prokaryotes and eukaryotes (but particularly the latter), has been particularly well represented. Although no one technical approach can be said to have brought the filed to its current level of sophistication, the ability to map the interactions of trans-acting factors with their DNA recognition sequences to a high level of precision has certainly been one of the more important advances. This "footprinting" approach has become almost ubiquitous in gene regulatory studies; however, it is in its "in vivo" application that ambiguities, confusions, and inconsistencies that may arise from a purely "in vitro"-based approach can often be resolved and placed in their proper perspective. Put more simply, that an interaction can be demonstrated to occur between purified factors and a particular piece of DNA in a test tube does not, of course, say anything regarding whether such interactions are occurring in vivo. The ability to probe for such interactions as they occur inside cells, with due attention paid to the relevant developmental stage, or to the tissue specificity of the interaction being probed, has made in vivo footprinting approach an invaluable adjunct to the "gene jockey's" arsenal of weapons.

Download or read book Stress Activated Protein Kinases written by Francesc Posas and published by Springer Science & Business Media. This book was released on 2008-01-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book leading researchers in the field discuss the state-of-the-art of many aspects of SAPK signaling in various systems from yeast to mammals. These include various chapters on regulatory mechanisms as well as the contribution of the SAPK signaling pathways to processes such as gene expression, metabolism, cell cycle regulation, immune responses and tumorigenesis. Written by international experts, the book will appeal to cell biologists and biochemists.

Download or read book Revealing the Linkage Between the Cellular Stress Response and Invasive Growth in the Yeast Saccharomyces Cerevisiae written by Eran Blachinsky and published by . This book was released on 2007 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: