Download or read book The Pyruvate Dehydrogenase Multienzyme Complex of Bacillus Subtilis written by J. A. Hodgson and published by . This book was released on 1983 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Wild type and Mutant Forms of the Pyruvate Dehydrogenase Multienzyme Complex from Bacillus Subtilis written by Jeffrey A. Hodgson and published by . This book was released on 1983 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Limited Proteolysis of the Pyruvate Dehydrogenase Multienzyme Complex of Bacillus Subtilis written by Peter N. Lowe and published by . This book was released on 1985 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Pyruvate Dehydrogenase Multienzyme Complex of Bacillus Stearothermophilus written by G. E. Henderson and published by . This book was released on 1979 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Another Possible Role for the E2 Subunit of the Pyruvate Dehydrogenase Complex of Bacillus Subtilis written by Andrew Stein and published by . This book was released on 1999 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Structural Domains in the E2 Component of the Pyruvate Dehydrogenase Multienzyme Complex from Bacillus Stearothermophilus written by Leonard C. Packman and published by . This book was released on 1991 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Structure and Molecular Biology of the Pyruvate Dehydrogenase Multienzyme Complex from Bacillus Stearothermophilus written by Adolfo Borges and published by . This book was released on 1990 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Pyruvate Dehydrogenase Multi enzyme Complex from Gram negative Bacteria written by Aart de Kok and published by . This book was released on 1998 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification of the Pyruvate Dehydrogenase Multienzyme Complex of Bacillus Stearothermophilus and Resolution of Its Four Component Plypeptides written by Christopher E. Henderson and published by . This book was released on 1980 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Interaction of Component Enzymes with the Peripheral Subunit binding Domain of the Pyruvate Dehydrogenase Multienzyme Complex of Bacillus Stearothermophilus written by Ivan A. D. Lessard and published by . This book was released on 1995 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Competitive Interaction of Component Enzymes with the Peripheral Subunit binding Domain of the Pyruvate Dehydrogenase Multienzyme Complex of Bacillus Stearothermophilus written by Ivan A. D. Lessard and published by . This book was released on 1996 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Kinetic and Structural Study of the Pyruvate Dehydrogenase Multienzyme Complex from Escherichia Coli written by Steven Ken Akiyama and published by . This book was released on 1981 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural Characterization and Subunit Communication of Escherichia Coli Pyruvate Dehydrogenase Multienzyme Complex written by Jaeyoung Song and published by . This book was released on 2011 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: The pyruvate dehydrogenase multienzyme complex (PDHc) from Escherichia coli (E. coli) is the best characterized of the 2-oxoacid dehydrogenase complexes. The complex plays a role as catalyst for the conversion of pyruvate to acetyl Coenzyme A (acetylCoA) by three enzyme components in the complex. The complex is comprised of 24 copies of the dimeric pyruvate dehydrogenase (E1ec; 99,474 Da), a cubic core of 24 copies of dihydrolipoamide acetyltransferase (E2ec; 65,959 Da), and 12 copies of dihydrolipoamide dehydrogenase (E3ec; 50,554 Da) (1-3). The crystal structure of the E. coli pyruvate dehydrogenase complex E1 subunit (E1ec) has been deterimined, and there were three missing regions (residues 1-55, 401-413, and 541-557) remaining absent in the model due to high flexibilities of these regions (4). Most bacterial pyruvate dehydrogenase complexes from either Gram-positive or Gram-negative bacteria have E1 components with an a2 homodimeric quaternary structure. In a sequel to our previous publications (5-8), the first NMR study on the flexible regions of the E1 component from Escherichia coli and its biological relevance was presented. In the study, sequence-specific NMR assignments for six residues in the N-terminal 1-55 region, and for a glycine in each of the two mobile active center loops of the E1 component, a 200 kDa homodimer was made. This was accomplished by using site-specific substitutions and appropriate labeling patterns, along with a peptide with the sequence corresponding to the N-terminal 1-35 amino acids of the E1 component. To study the functions of these mobile regions, the spectra were also examined in the presence of: (a) a reaction intermediate analog known to affect the mobility of the active center loops, (b) an E2 component construct consisting of a lipoyl domain (LD) and peripheral subunit binding domain (PSBD) and (c) a peptide corresponding to the amino acid sequence of the E2 peripheral subunit binding domain. Deductions from the NMR studies are in excellent agreement with our functional finding, providing clear indication that the N-terminal region of the E1 interacts with the E2 peripheral subunit binding domain, and that this interaction precedes reductive acetylation. The results provide the first structural support to the notion that the N-terminal region of the E1 component of this entire class of bacterial pyruvate dehydrogenase complexes is responsible for binding the E2 component. Among three components of PDHc, E2ec consists of 24 chains, and in the overall reaction, the lipoyl domain is reductively acetylated by E1ec and pyruvate, and S-acetyldihydrolipoyl domain transfers acetyl group to Coenzyme A leading acetylcoenzyme A production. Even though the precise number of E2 subunits is still ambiguous, in many cases including human PHDc (9), the sum is a multiple of three chains indicating that multiples of chains can affect the acetyl transfer to CoA by interchain acetyl transfer. To answer this question, the E2 component from E. coli specifically designed with only a single lipoyl domain (LD, 1-lip E2ec), rather than the three lipoyl domains found in the wild type enzyme (3-lip E2ec) was used. Earlier, it was shown that the activities of these two enzymes are virtually the same, while the 1-lip E2ec provides obvious advantages for mechanistic studies (10). At the same time, it is also important to point out that there indeed are other sources of the E2 component with only a single domain, such as from Mycobacterium tuberculosis. To study the question, two constructs of the 1-lip E2ec were prepared, one in which the lysine on the LD ordinarily carrying the lipoic acid is changed to alanine (henceforth K41A), and a second one in which the histidine believed to catalyze the transacetylation in the catalytic domain (CD) is substituted to A or C, H399C and H399A. The first is incompetent towards posttranslational ligation of the lipoic acid, hence towards reductive acetylation. The second one is incompetent towards acetylCoA formation, by virtue of the absence of the catalytic histidine residue. This is a biochemical version of a classical crossover experiment, as should the reaction proceed within one chain the two constructs should each be inactive either together or individually. On the other hand, should the reaction proceed by an interchain mechanism, addition of the two constructs should produce measurable activity. Both kinetic and mass spectrometric evidence supported the second scenario. Hence, plausible model/explanation for the multiples of three chains present in each E2 component as well as for their assembly was suggested.
Download or read book Intramolecular Coupling of Active Sites in the Pyruvate Dehydrogenase Multienzyme Complexes from Bacterial and Mammalian Sources written by Christopher J. Stanley and published by . This book was released on 1981 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Pyruvate Dehydrogenase Complex from Bacillus written by Jaap Visser and published by . This book was released on 1982 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of the Pyruvate Dehydrogenase Multienzyme Complex written by R. H. Behal and published by . This book was released on 1993 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Studies on the Bacillus Subtilis Succinate Dehydrogenase Complex written by Kerstin Magnusson and published by . This book was released on 1987 with total page 27 pages. Available in PDF, EPUB and Kindle. Book excerpt:
