Download or read book The Purification and Characterization of the Escherichia Coli Helicase II Protein and a Study of the Protein s Enzymatic Activities written by Gregory Thomas Runyon and published by . This book was released on 1991 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of the Escherichia Coli Rep Helicase DNA Unwinding Activity and the Effect of Two DNA Binding Proteins on this Reaction written by Janet Elizabeth Yancey-Wrona and published by . This book was released on 1991 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Characterization of Penicillin binding Protein 2 of Escherichia Coli written by Ora K. Dillon-Carter and published by . This book was released on 1983 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Characterization of the 50S Ribosomal Proteins from Escherichia Coli written by Guido Carlos Mora and published by . This book was released on 1971 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Purification and Analysis II written by Iconcept Press and published by Iconcept Press. This book was released on 2014-06-02 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form, facilitating a biological function. A polypeptide is a single linear polymer chain of amino acids bonded together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues. The sequence of amino acids in a protein is defined by the sequence of a gene, which is encoded in the genetic code. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. "Protein Purification and Analysis" discusses varies aspect related to protein analysis. There are totally three volumes. This book is the second volume. Chapter 1 describes protein-based methods for the analysis of plant alcohol dehydrogenases. Chapter 2 demonstrates production of recombinant fungal cell wall-degrading enzymes and their tag-affinity purification and biochemical analyses. Cell wall-degrading enzymes act on cleaving glycosidic bonds of polysaccharides and oligosaccharides, affecting morphological changes, plant-microbe interactions and nutrient acquisition. Chapter 3 contains a number of methodologies including recombinant protein purification and analysis, enzymatic reporter assays and fluorescent tag detection. Chapter 4 allows the reader to become acquainted with methods of recombinant expression, purification and determination of the level of activity of staphylococcal epidermolytic toxins. Chapter 5 discussed the recombinant expression, purification and biochemical analysis of a variety of extremophilic enzymes with potential industrial application. Chapter 6 discusses tellurite, which is highly toxic for most living organisms. The chapter describes how the mechanism by which this oxyanion exerts its toxicity can be assessed by studying the effect of some metabolic enzymes which seem to help in detoxifying the toxicant. Chapter 7 describes the principle of, devices used for, protocol for, and mechanism underlying gene introduction. Chapter 8 outlines a SELEX method for the discovery of a target-specific aptamer. The aptamer is then used to purify the target (SEB) from a mixture of closely related enterotoxins using non-fat dry milk as a representative food matrix. Chapter 9 proposes an overview of the methodologies employed for the manipulation of membrane protein transporters, from their purification to their reconstitution into proteoliposomes. The authors presented an original approach they developed for the functional study of a multidrug efflux pump responsible for the active transport of antibiotics in bacteria. Chapter 10 is about the versatility of substrate analogues containing unnatural amino acids in the challenging study of peptidyl-aminoacyl-L/D-isomerases. Enzymes of this class catalyze an exciting post-translational reaction, namely the change of chirality of amino acids within peptide linkage whereby an L-amino acid is converted to the D-isomer. Chapter 11 investigates the the effects of combined heat and pressure on whole beef muscle proteins and isolated myofibril solubility and protein electrophoretic pattern. It attempts to understand the relative effects of heat and pressure treatments on the proteins of beef muscle. Chapter 12 reviews the normal synovium including it's microscopic structure, cell origins and recruitment, function and its clinical relevance as a target of immunologic disease.
Download or read book Escherichia Coli DNA Helicase II written by Mark Christopher Hall and published by . This book was released on 1998 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Modern Analytical Ultracentrifugation written by Todd M. Schuster and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.
Download or read book The Purification and Characterization of a DNA binding Protein Induced by Infection of Escherichia Coli with Bacteriophage written by Roberta Charlotte Reuben and published by . This book was released on 1973 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Production Purification and Characterization of the Escherichia Coli Kch Protein written by Dániel Ungár and published by . This book was released on 1999 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2098 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book DNA Replication Controls Volume 1 written by Eishi Noguchi and published by MDPI. This book was released on 2018-04-27 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "DNA Replication Controls" that was published in Genes
Download or read book Activity Report written by International Centre for Genetic Engineering and Biotechnology and published by . This book was released on 2002 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Advances in Protein Biochemistry written by and published by . This book was released on 1997 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1987 with total page 1398 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Helicases written by and published by Academic Press. This book was released on 2012-11-13 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. - This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases - The contributions in this volume cover the broad scope of methods in the research on these enzymes
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
