Download or read book The Structural Heterogeneity of the 30S Ribosomal Subunit from Escherichia Coli written by Paul Voynow and published by . This book was released on 1970 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Proteins of the 30S Ribosomal Subunit from Escherichia Coli written by Simon John Stollard Hardy and published by . This book was released on 1969 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies of Functional Domains in Ribosomal Proteins of the Escherichia Coli 30S Ribosomal Subunit written by Richard C. Conrad and published by . This book was released on 1987 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Construction and Comparison of Models of the Escherichia Coli 3OS Ribosomal Subunit written by Philip Thomas Gaffney and published by . This book was released on 1979 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Studies of 30S Ribosomal Proteins of Escherichia Coli written by Edward Asahel Birge and published by . This book was released on 1970 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Escherichia Coli 30S Ribosomal Subunit Assembly written by Jennifer Anne Maki and published by . This book was released on 2004 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: The prokaryotic ribosome is a 2.5 MDa particle comprised of two asymmetric subunits, the large (50S) and small (30S) subunits. The large subunit contains two RNAs (5S and 23S) in addition to thirty-four proteins. The small subunit consists of one RNA (16S rRNA) and twenty-one proteins. Although the crystal structure has been solved, much remains to be revealed concerning the assembly of this macromolecular structure. Our laboratory is focused on the assembly of the small subunit. In vitro assembly of this structure was achieved in the late 1960's and early 1970's. At low temperature when 16S rRNA and all of the small subunit proteins are incubated together, only a subset of the proteins are able to associate with the RNA and a particle termed Reconstitution Intermediate (RI, 2IS) results. When RI particles are heat treated, a conformational rearrangement occurs and RI* (26S) particles result that are capable of complete assembly with the remainder of the small subunit proteins, even at low temperature, to form functional 30S subunits. In vitro 30S subunit assembly requires long incubation periods, high ionic strength, and heat treatment. In light of these strict requirements, we hypothesized that assembly factors must exist in vivo to facilitate this crucial assembly process, making it accurate and efficient. We have identified the DnaK chaperone system as one such factor. The purified DnaK chaperone system is sufficient to facilitate in vitro 30S subunit assembly at low temperature, forming 30S particles that co-sediment, have the same protein complement, bind tRNA, and participate in polyphenylalanine synthesis like 30S subunits. Additionally, the association behavior of the DnaK chaperone system components with pre-30S particles in vitro was observed and found to be very similar to their association with substrate in their well-characterized protein folding role. Lastly, it was determined that DnaK binds small subunit components in vivo, including pre-processed 16S rRNA. This is the first evidence clearly demonstrating a direct link between the DnaK chaperone system and the assembly of ribosomes in E. coli, and the first instance in which an extra-ribosomal assembly factor has been shown to facilitate 30S subunit assembly in vitro.
Download or read book Chemical Modification of the Escherichia Coli 30s Ribosomal Subunit written by Thomas Guy Fanning and published by . This book was released on 1970 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Modification of the Messenger RNA Binding Site on the 30S Ribosomal Subunits of Escherichia Coli written by Chu Chang and published by . This book was released on 1977 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein RNA Interactions in the Escherichia Coli 30S Ribosomal Subunit written by Jean E. Schwarzbauer and published by . This book was released on 1980 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Several Contributions to the Understanding of Ribosome Structure written by Eleanor Spicer Ward and published by . This book was released on 1975 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on the Cistron Selectivity of the 30s Ribosomal Subunit of Escherichia Coli in the Translation of Natural Messenger RNA written by William Robert Gette and published by . This book was released on 1976 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on the Anatomy of Ribosomes from Escherichia Coli written by Pallaiah Thammana and published by . This book was released on 1974 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Translation Initiation Reconstituted Systems and Biophysical Methods written by and published by Academic Press. This book was released on 2007-10-23 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.
Download or read book Assembly of the 30S Ribosomal Subunit written by Joel F. Grondek and published by . This book was released on 2004 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of Escherichia coli 30S ribosomal subunit assembly have revealed a hierarchical and cooperative association of ribosomal proteins with 16S ribosomal RNA; these results have been used to compile an in vitro 30S subunit assembly map. In single protein addition and omission studies, ribosomal protein S13 was shown to be dependent on the prior association of ribosomal protein S20 for binding to the ribonucleoprotein particle. While the overwhelming majority of interactions revealed in the assembly map are consistent with additional data, the dependency of S13 on S20 is not. Structural studies position S13 in the head of the 30S subunit over 100Å away from S20, which resides near the bottom of the body of the 30S subunit. All of the proteins that reside in the head of the 30S subunit, except S13, have been shown to be part of the S7 assembly branch, that is, they all depend on S7 for association with the assembling 30S subunit. Given these observations, the assembly requirements for S13 were investigated using base-specific chemical footprinting and primer extension analysis. These studies reveal that S13 can bind to 16S rRNA in the presence of S7, but not S20. Additionally, polyspecific interaction between S13 and other members of the S7 assembly branch have been observed. These results link S13 to the 3' major domain family of proteins, and the S7 assembly branch, placing S13 in a new location in the 30S subunit assembly map where its position is in accordance with much biochemical and structural data.
Download or read book Protein Composition of Ribosomal Binding Sites for Initiation Factors in Escherichia Coli written by Ronald Lee Heimark and published by . This book was released on 1976 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Studies on the Conformation of the 30S Ribosome from E Coli written by Marina Kit-tsing Lam and published by . This book was released on 1977 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ribosomes written by Alexander Spirin and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on an advanced course of lectures on ribosome structure and protein biosynthesis that I offer at the Moscow State University. These lectures have been part of a general course on molecular biology for almost three decades, and they have undergone considerable evolution as knowledge has been pro gressing in this field. The progress continues, and readers should be prepared that some facts, statements, and ideas included in the book may be incomplete or out of-date. In any case, this is primarily a textbook, but not a comprehensive review. It provides a background of knowledge and current ideas in the field and gives ex amples of observations and their interpretations. I understand that some interpre tations and generalizations may be tentative or disputable, but I hope that this will stimulate thinking and discussing better than if I left white spots. The book has a prototype: it is my monograph "Ribosome Structure and Pro tein Biosynthesis" published by the Benjamin/Cummings Publishing Company, Menlo Park, California, in 1986. Here I have basically kept the former order of pre sentation ofthe topics and the subdivision into chapters. The contents ofthe chap ters, however, have been significantly revised and supplemented. The newly writ ten chapters on translational control in prokaryotes (Chapter 16) and eukaryotes (Chapter 17) are added.
