Download or read book The Physiological Properties of Plant Protoplasts written by Paul-Emile Pilet and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea for the p~esent book arose from a 3-day seminar which I organized in March 1984 for young research workers in plant physiology. Participants came from several universities of the French-speaking part of Switzerland and speakers from Basel, Mtinchen, Nottingham, Perpignan, Regensburg, Sheffield, Toulouse, Yale, ZUrich ... and Lausannne. The theme of the seminar was chosen from the range of research fields of our Institute. Meanwhile, feeling it was important to bear in mind that some of our hearers were not specialists in the chosen topic, I wished to pro vide a subject that would be of scientific, methodological and epistemologi cal interest. The critical analysis of the structural and functional characteristics of plant protoplasts exactly met these criteria. There exists ample material for discussion of the techniques of protoplast preparation, methods used in morphological, biological and biochemical studies, and for the comparison of protoplasts with the cells from which they are obtained.
Download or read book The Physiological Properties of Plant Protoplasts Proceedings in Life Sciences written by P. E. Pilet and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Some Physiological Properties of Plant Protoplasts written by and published by . This book was released on 1983 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Molecular Biology of Plant Cells written by H. Smith and published by Univ of California Press. This book was released on 1977-01-01 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
Download or read book Plant Protoplasts written by Fowke and published by CRC Press. This book was released on 2018-01-18 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.
Download or read book Plant Protoplasts written by Tessa Bengochea and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.
Download or read book Plant Protoplasts and Genetic Engineering I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.
Download or read book Introduction to Plant Physiology written by William G. Hopkins and published by . This book was released on 2004 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells, tissues, and organs: the architecture of plants; The plant cell building blocks: lipids, proteins, and carbohydrates; Lipids are a class of molecules that includes fats, oils, sterols, and pigments; Proteins playa central role in the biochemistry of cells and are responsible for virtually all the properties of life as we know it; Carbohydrates are the most abundant class of biological molecules; Biological membranes; The membrane lipid forms a bilayer, a highly fluid but very stable structure; Membranes contain significantamounts of protein; Cellular organelles; Most mature plant cells contain a large, central vacuole; The nucleus is the information center of the cell; The endoplasmic reticulum and golgi apparatus are centers of membrane biosynthesis and secretory activities; The mitochondrion is the principal site of cellular respiration; Plastids are a family of organelles with a variety of functions; Microbodies are metabolically very active; Cytoskeleton the extracellular matrix; The primary cell wall is a flexible n etwork of cellulose microfibrils and cross-linking glycans; The cellulose-glycan lattice is embedded in a matrix of pectin and protein; Cellulose microfibrils are assembled at the plasma membrane as they are extruded into the cell wall; The secondary cell wall is deposited on the inside of the primary wall in maturing cells; Plasmadesmata are cytoplasmic channels extend through the wall to connect the protoplasts of adjacent cells; Tissues and organs; Tissues are groups of cells that form organized, functional unit; Meristems are regions of perpetually dividing cells; Parenchyma is the most abundant living tissue in plants; Supporting tissues are distributed throughout the primary and secondary plant bodies; Vascular tissues are the principal conducting tissues for water and nutrients ; Epidermis is a superficial tissue that f orms a continuous layer over the surface of the primary; Plant body; Plant organs; Roots anchor the plant and absorb water and minerais from the soil.
Download or read book Progress in Plant Protoplast Research written by K.J. Puite and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987
Download or read book Biochemistry and Function of Vacuolar Adenosine Triphosphatase in Fungi and Plants written by B.P. Marin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Protoplasts and Genetic Engineering written by Y. P. S. Bajaj and published by Springer. This book was released on 1989 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Tissue Culture written by Roberta H. Smith and published by Academic Press. This book was released on 2013 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Download or read book Plant Protoplasts and Genetic Engineering II written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1972 with total page 1302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plants Under Stress written by Hamlyn G. Jones and published by Cambridge University Press. This book was released on 1989-10-27 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume identifies how stressful conditions affect plants. Various stresses can have a major impact on plant growth and survival. This book examines some of the more important stresses, shows how they affect the plant and then reviews how new varieties or new species can be selected which are less vulnerable to stress.
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1985-10 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Tissue Culture written by Robert H. Smith and published by Academic Press. This book was released on 2012-12-02 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
