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Book The Science and Applications of Microbial Genomics

Download or read book The Science and Applications of Microbial Genomics written by Institute of Medicine and published by National Academies Press. This book was released on 2013-05-02 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past several decades, new scientific tools and approaches for detecting microbial species have dramatically enhanced our appreciation of the diversity and abundance of the microbiota and its dynamic interactions with the environments within which these microorganisms reside. The first bacterial genome was sequenced in 1995 and took more than 13 months of work to complete. Today, a microorganism's entire genome can be sequenced in a few days. Much as our view of the cosmos was forever altered in the 17th century with the invention of the telescope, these genomic technologies, and the observations derived from them, have fundamentally transformed our appreciation of the microbial world around us. On June 12 and 13, 2012, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to discuss the scientific tools and approaches being used for detecting and characterizing microbial species, and the roles of microbial genomics and metagenomics to better understand the culturable and unculturable microbial world around us. Through invited presentations and discussions, participants examined the use of microbial genomics to explore the diversity, evolution, and adaptation of microorganisms in a wide variety of environments; the molecular mechanisms of disease emergence and epidemiology; and the ways that genomic technologies are being applied to disease outbreak trace back and microbial surveillance. Points that were emphasized by many participants included the need to develop robust standardized sampling protocols, the importance of having the appropriate metadata, data analysis and data management challenges, and information sharing in real time. The Science and Applications of Microbial Genomics summarizes this workshop.

Book Marine Anthropogenic Litter

Download or read book Marine Anthropogenic Litter written by Melanie Bergmann and published by Springer. This book was released on 2015-06-01 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes how man-made litter, primarily plastic, has spread into the remotest parts of the oceans and covers all aspects of this pollution problem from the impacts on wildlife and human health to socio-economic and political issues. Marine litter is a prime threat to marine wildlife, habitats and food webs worldwide. The book illustrates how advanced technologies from deep-sea research, microbiology and mathematic modelling as well as classic beach litter counts by volunteers contributed to the broad awareness of marine litter as a problem of global significance. The authors summarise more than five decades of marine litter research, which receives growing attention after the recent discovery of great oceanic garbage patches and the ubiquity of microscopic plastic particles in marine organisms and habitats. In 16 chapters, authors from all over the world have created a universal view on the diverse field of marine litter pollution, the biological impacts, dedicated research activities, and the various national and international legislative efforts to combat this environmental problem. They recommend future research directions necessary for a comprehensive understanding of this environmental issue and the development of efficient management strategies. This book addresses scientists, and it provides a solid knowledge base for policy makers, NGOs, and the broader public.

Book Fundamentals of Ecosystem Science

Download or read book Fundamentals of Ecosystem Science written by Kathleen C. Weathers and published by Academic Press. This book was released on 2021-07-23 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Ecosystem Science, Second Edition provides a comprehensive introduction to modern ecosystem science covering land, freshwater and marine ecosystems. Featuring full color images to support learning and written by a group of experts, this updated edition covers major concepts of ecosystem science, biogeochemistry, and energetics. Case studies of important environmental problems offer personal insights into how adopting an ecosystem approach has helped solve important intellectual and practical problems. For those choosing to use the book in a classroom environment, or who want to enrich further their reading experience, teaching and learning assets are available at Elsevier.com. - Covers both aquatic (freshwater and marine) and terrestrial ecosystems with updated information - Includes a new chapter on microbial biogeochemistry - Features vignettes throughout the book with real examples of how an ecosystem approach has led to important change in policy, management, and ecological understanding - Demonstrates the application of an ecosystem approach in synthesis chapters and case studies - Contains new coverage of human-environment interactions

Book Forensic Microbiology

    Book Details:
  • Author : David O. Carter
  • Publisher : John Wiley & Sons
  • Release : 2017-03-27
  • ISBN : 1119062578
  • Pages : 424 pages

Download or read book Forensic Microbiology written by David O. Carter and published by John Wiley & Sons. This book was released on 2017-03-27 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic Microbiology focuses on newly emerging areas of microbiology relevant to medicolegal and criminal investigations: postmortem changes, establishing cause of death, estimating postmortem interval, and trace evidence analysis. Recent developments in sequencing technology allow researchers, and potentially practitioners, to examine microbial communities at unprecedented resolution and in multidisciplinary contexts. This detailed study of microbes facilitates the development of new forensic tools that use the structure and function of microbial communities as physical evidence. Chapters cover: Experiment design Data analysis Sample preservation The influence of microbes on results from autopsy, toxicology, and histology Decomposition ecology Trace evidence This diverse, rapidly evolving field of study has the potential to provide high quality microbial evidence which can be replicated across laboratories, providing spatial and temporal evidence which could be crucial in a broad range of investigative contexts. This book is intended as a resource for students, microbiologists, investigators, pathologists, and other forensic science professionals.

Book Freshwater Ecology

    Book Details:
  • Author : Walter K. Dodds
  • Publisher : Academic Press
  • Release : 2010-11-03
  • ISBN : 0080884776
  • Pages : 840 pages

Download or read book Freshwater Ecology written by Walter K. Dodds and published by Academic Press. This book was released on 2010-11-03 with total page 840 pages. Available in PDF, EPUB and Kindle. Book excerpt: Freshwater Ecology, Second Edition, is a broad, up-to-date treatment of everything from the basic chemical and physical properties of water to advanced unifying concepts of the community ecology and ecosystem relationships as found in continental waters.With 40% new and expanded coverage, this text covers applied and basic aspects of limnology, now with more emphasis on wetlands and reservoirs than in the previous edition. It features 80 new and updated figures, including a section of color plates, and 500 new and updated references. The authors take a synthetic approach to ecological problems, teaching students how to handle the challenges faced by contemporary aquatic scientists.This text is designed for undergraduate students taking courses in Freshwater Ecology and Limnology; and introductory graduate students taking courses in Freshwater Ecology and Limnology. - Expanded revision of Dodds' successful text. - New boxed sections provide more advanced material within the introductory, modular format of the first edition. - Basic scientific concepts and environmental applications featured throughout. - Added coverage of climate change, ecosystem function, hypertrophic habitats and secondary production. - Expanded coverage of physical limnology, groundwater and wetland habitats. - Expanded coverage of the toxic effects of pharmaceuticals and endocrine disrupters as freshwater pollutants - More on aquatic invertebrates, with more images and pictures of a broader range of organisms - Expanded coverage of the functional roles of filterer feeding, scraping, and shredding organisms, and a new section on omnivores. - Expanded appendix on standard statistical techniques. - Supporting website with figures and tables - http://www.elsevierdirect.com/companion.jsp?ISBN=9780123747242

Book Groundwater Surface Water Interactions

Download or read book Groundwater Surface Water Interactions written by Habil. Jörg Lewandowski and published by MDPI. This book was released on 2020-12-10 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent years have seen a paradigm shift in our understanding of groundwater–surface water interactions: surface water and aquifers were long considered discrete, separate entities; they are now understood as integral components of a surface–subsurface continuum. This book provides an overview of current research advances and innovative approaches in groundwater–surface water interactions. The 20 research articles and 1 communication cover a wide range of thematic scopes, scales, and experimental and modelling methods across different disciplines (hydrology, aquatic ecology, biogeochemistry, and environmental pollution). The book identifies current knowledge gaps and reveals the challenges in establishing standardized measurement, observation, and assessment approaches. It includes current hot topcis with environmental and societal relevance such as eutrophication, retention of legacy, and emerging pollutants (e.g., pharmaceuticals and microplastics), urban water interfaces, and climate change impacts. The book demonstrates the relevance of processes at groundwater–surface water interfaces for (1) regional water balances and (2) quality and quantity of drinking water resources. As such, this book represents the long-awaited transfer of the above-mentioned paradigm shift in understanding of groundwater–surface water interactions from science to practice.

Book Cyanobacterial Harmful Algal Blooms  State of the Science and Research Needs

Download or read book Cyanobacterial Harmful Algal Blooms State of the Science and Research Needs written by H. Kenneth Hudnell and published by Springer Science & Business Media. This book was released on 2008-03-13 with total page 955 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the ever-increasing incidence of harmful cyanobacterial algal blooms, this monograph has added urgency and will be essential reading for all sorts of researchers, from neuroscientists to cancer research specialists. The volume contains the proceedings of the 2005 International Symposium on Cyanobacterial Harmful Algal Blooms, and has been edited by H. Kenneth Hudnell, of the US Environmental Protection Agency. It contains much of the most recent research into the subject.

Book Recarbonization of the Biosphere

Download or read book Recarbonization of the Biosphere written by Rattan Lal and published by Springer Science & Business Media. This book was released on 2012-03-28 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human activities are significantly modifying the natural global carbon (C) cycles, and concomitantly influence climate, ecosystems, and state and function of the Earth system. Ever increasing amounts of carbon dioxide (CO2) are added to the atmosphere by fossil fuel combustion but the biosphere is a potential C sink. Thus, a comprehensive understanding of C cycling in the biosphere is crucial for identifying and managing biospheric C sinks. Ecosystems with large C stocks which must be protected and sustainably managed are wetlands, peatlands, tropical rainforests, tropical savannas, grasslands, degraded/desertified lands, agricultural lands, and urban lands. However, land-based sinks require long-term management and a protection strategy because C stocks grow with a progressive improvement in ecosystem health.

Book Microbial Interventions in Agriculture and Environment

Download or read book Microbial Interventions in Agriculture and Environment written by Dhananjaya Pratap Singh and published by Springer Nature. This book was released on 2019-11-27 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial communities and their functions play a crucial role in the management of ecological, environmental and agricultural health on the Earth. Microorganisms are the key identified players for plant growth promotion, plant immunization, disease suppression, induced resistance and tolerance against stresses as the indicative parameters of improved crop productivity and sustainable soil health. Beneficial belowground microbial interactions with the rhizosphere help plants mitigate drought and salinity stresses and alleviate water stresses under the unfavorable environmental conditions in the native soils. Microorganisms that are inhabitants of such environmental conditions have potential solutions for them. There are potential microbial communities that can degrade xenobiotic compounds, pesticides and toxic industrial chemicals and help remediate even heavy metals, and thus they find enormous applications in environmental remediation. Microbes have developed intrinsic metabolic capabilities with specific metabolic networks while inhabiting under specific conditions for many generations and, so play a crucial role. The book Microbial Interventions in Agriculture and Environment is an effort to compile and present a great volume of authentic, high-quality, socially-viable, practical and implementable research and technological work on microbial implications. The whole content of the volume covers protocols, methodologies, applications, interactions, role and impact of research and development aspects on microbial interventions and technological outcomes in prospects of agricultural and environmental domain including crop production, plan-soil health management, food & nutrition, nutrient recycling, land reclamation, clean water systems and agro-waste management, biodegradation & bioremediation, biomass to bioenergy, sanitation and rural livelihood security. The covered topics and sub-topics of the microbial domain have high implications for the targeted and wide readership of researchers, students, faculty and scientists working on these areas along with the agri-activists, policymakers, environmentalists, advisors etc. in the Government, industries and non-government level for reference and knowledge generation.

Book Essential Microbiology

    Book Details:
  • Author : Stuart Hogg
  • Publisher : John Wiley & Sons
  • Release : 2013-06-10
  • ISBN : 1119978912
  • Pages : 534 pages

Download or read book Essential Microbiology written by Stuart Hogg and published by John Wiley & Sons. This book was released on 2013-06-10 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essential Microbiology 2nd Edition is a fully revised comprehensive introductory text aimed at students taking a first course in the subject. It provides an ideal entry into the world of microorganisms, considering all aspects of their biology (structure, metabolism, genetics), and illustrates the remarkable diversity of microbial life by devoting a chapter to each of the main taxonomic groupings. The second part of the book introduces the reader to aspects of applied microbiology, exploring the involvement of microorganisms in areas as diverse as food and drink production, genetic engineering, global recycling systems and infectious disease. Essential Microbiology explains the key points of each topic but avoids overburdening the student with unnecessary detail. Now in full colour it makes extensive use of clear line diagrams to clarify sometimes difficult concepts or mechanisms. A companion web site includes further material including MCQs, enabling the student to assess their understanding of the main concepts that have been covered. This edition has been fully revised and updated to reflect the developments that have occurred in recent years and includes a completely new section devoted to medical microbiology. Students of any life science degree course will find this a concise and valuable introduction to microbiology.

Book Intermittent Rivers and Ephemeral Streams

Download or read book Intermittent Rivers and Ephemeral Streams written by Thibault Datry and published by Academic Press. This book was released on 2017-07-11 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intermittent Rivers and Ephemeral Streams: Ecology and Management takes an internationally broad approach, seeking to compare and contrast findings across multiple continents, climates, flow regimes, and land uses to provide a complete and integrated perspective on the ecology of these ecosystems. Coupled with this, users will find a discussion of management approaches applicable in different regions that are illustrated with relevant case studies. In a readable and technically accurate style, the book utilizes logically framed chapters authored by experts in the field, allowing managers and policymakers to readily grasp ecological concepts and their application to specific situations. - Provides up-to-date reviews of research findings and management strategies using international examples - Explores themes and parallels across diverse sub-disciplines in ecology and water resource management utilizing a multidisciplinary and integrative approach - Reveals the relevance of this scientific understanding to managers and policymakers

Book Bacterial Adhesion

    Book Details:
  • Author : Madilyn Fletcher
  • Publisher : John Wiley & Sons
  • Release : 1996-09-21
  • ISBN : 9780471021858
  • Pages : 378 pages

Download or read book Bacterial Adhesion written by Madilyn Fletcher and published by John Wiley & Sons. This book was released on 1996-09-21 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: BACTERIAL ADHESION Molecular and Ecological Diversity Edited byMadilyn Fletcher Over the last twenty years, research has revealed the enormouscomplexity underlying the phenomenon of bacterial adhesion. Theinitial research goal was to understand the mechanism of attachmentand its effects on the bacteria as well as the host. As researchprogressed, however, it became evident that many differentattachment mechanisms exist. These diverse forms of adhesion arethe results of numerous evolutionary pressures, and each may bepart of a larger behavioral strategy. This comprehensive overview details how diversity in habitat andecological requirements has led to enormous variety in adhesivecell components, underlying genetic determinants, and behavioralstrategies. It presents the latest research on adhesion mechanismsand strategies found in diverse environments and microorganisms,including the new environment of biomaterials. Bacterial Adhesion: Molecular and Ecological Diversity examinesadhesion as a strategy for nutrient access and as a phase in thecomplex behavior of life cycle processes. It covers the latestresearch and innovative approaches in the field, including: * Conceptual advances in research on the adhesion of bacteria tooral surfaces * Enhancing colonization in a fluctuating environment * The cellulosome: a cell-surface organelle for the adhesion to anddegradation of cellulose * Pseudomonas aeruginosa--versatile attachment mechanisms * Sensing, response, and adaptation to surfaces * Myxococcus coadhesion and role in the life cycle Edited by a leading authority on bacterial adhesion and featuringcontributions from the field's leading experts, this book speaks toresearchers in all areas of microbiology, biotechnology,environmental technology, and environmental science, as well asurology, immunology, and infectious diseases. Bacterial Adhesion: Molecular and Ecological Diversity is thelatest addition to the Wiley Series in Ecological and AppliedMicrobiology. Recent books in the series include: * Microbial Transformation and Degradation of Toxic OrganicChemicals edited by Lily Y. Young and Carl E. Cerniglia * Wastewater Microbiology by Gabriel Bitton * Environmental Microbiology edited by Ralph Mitchell * Biotechnology in Plant Disease Control edited by Ilan Chet * Antarctic Microbiology edited by E. Imre Friedmann * Effects of Acid Rain on Forest Processes edited by Douglas L.Godbold and Aloys Huttermann

Book Microbiomes of the Built Environment

    Book Details:
  • Author : National Academies of Sciences, Engineering, and Medicine
  • Publisher : National Academies Press
  • Release : 2017-10-06
  • ISBN : 0309449839
  • Pages : 318 pages

Download or read book Microbiomes of the Built Environment written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2017-10-06 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: People's desire to understand the environments in which they live is a natural one. People spend most of their time in spaces and structures designed, built, and managed by humans, and it is estimated that people in developed countries now spend 90 percent of their lives indoors. As people move from homes to workplaces, traveling in cars and on transit systems, microorganisms are continually with and around them. The human-associated microbes that are shed, along with the human behaviors that affect their transport and removal, make significant contributions to the diversity of the indoor microbiome. The characteristics of "healthy" indoor environments cannot yet be defined, nor do microbial, clinical, and building researchers yet understand how to modify features of indoor environmentsâ€"such as building ventilation systems and the chemistry of building materialsâ€"in ways that would have predictable impacts on microbial communities to promote health and prevent disease. The factors that affect the environments within buildings, the ways in which building characteristics influence the composition and function of indoor microbial communities, and the ways in which these microbial communities relate to human health and well-being are extraordinarily complex and can be explored only as a dynamic, interconnected ecosystem by engaging the fields of microbial biology and ecology, chemistry, building science, and human physiology. This report reviews what is known about the intersection of these disciplines, and how new tools may facilitate advances in understanding the ecosystem of built environments, indoor microbiomes, and effects on human health and well-being. It offers a research agenda to generate the information needed so that stakeholders with an interest in understanding the impacts of built environments will be able to make more informed decisions.

Book Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria

Download or read book Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria written by Rainer Kurmayer and published by John Wiley & Sons. This book was released on 2017-09-05 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

Book Encyclopedia of Environmental Science and Engineering  Sixth Edition  Print Version

Download or read book Encyclopedia of Environmental Science and Engineering Sixth Edition Print Version written by Edward N. Ziegler and published by CRC Press. This book was released on 2012-06-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The authors ... continue the pursuit of new knowledge, calculated to bring new fruits of health, safety, and comfort to man and his environs. The charms, as well as the subtle hazards, of the terms ‘conservation, preservation, and ecology’ need to be crystallized so that the public and their decision-makers practice this complex art with clearer conception and perception than is apparent in recent bitter confrontations." —From the Foreword to the Fourth Edition by Abel Wolman What’s New in This Edition: New entries on environmental and occupational toxicology, geoengineering, and lead abatement Twenty-five significantly updated entries, including expanded discussion of water supplies and waste water treatment, biomass and renewable energy, and international public health issues An expanded list of acronyms and abbreviations Encyclopedia of Environmental Science and Engineering, Sixth Edition is still the most comprehensive, authoritative reference available in the field. This monumental two-volume encyclopedia now includes entries on topics ranging from acid rain, air pollution, and community health to environmental law, instrumentation, modeling, alternative energy, radioactive waste, and water treatment. The broad coverage includes highly specialized topics as well as those that transcend traditional disciplinary boundaries, reflecting the interdisciplinary skills and knowledge required by environmental researchers and engineers. Featuring expert contributors representing industry, academia, and government agencies, the encyclopedia presents fundamental concepts and applications in environmental science and engineering. The entries are supported by extensive figures, photographs, tables, and equations. This sixth edition includes new material on water supplies and wastewater treatment, biomass and renewable energy, and international public health issues. New entries cover environmental and occupational toxicology, geoengineering, and lead abatement. The Encyclopedia of Environmental Science and Engineering provides a view of the field that helps readers understand, manage, and respond to threats to the human environment. Contact us to inquire about subscription options and print/online combination packages. US: (Tel) 1.888.318.2367 / (email) [email protected] International: (Tel) +44 (0) 20 7017 6062 / (email) [email protected]

Book Wildlife Biodiversity Conservation

Download or read book Wildlife Biodiversity Conservation written by Susan C. Underkoffler and published by Springer Nature. This book was released on 2021-06-26 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the multidisciplinary challenges in biodiversity conservation with a focus on wildlife crime and how forensic tools can be applied to protect species and preserve ecosystems. Illustrated by numerous case studies covering different geographical regions and species the book introduces to the fundamentals of biodiversity conflicts, outlines the unique challenges of wildlife crime scenes and reviews latest techniques in environmental forensics, such as DNA metagenomics. In addition, the volume explores the socio-economic perspective of biodiversity protection and provides an overview of national and international conservation laws. The field of conservation medicine stresses the importance of recognizing that human health, animal health, and ecosystem health are inextricably interdependent and the book serves as important contribution towards achieving the UN Sustainable Developmental Goals, in particular SDG 15, Life on Land. The book addresses graduate students, scientists and veterinary professionals working in wildlife research and conservation biology.

Book Drinking Water Distribution Systems

Download or read book Drinking Water Distribution Systems written by National Research Council and published by National Academies Press. This book was released on 2006-12-22 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protecting and maintaining water distributions systems is crucial to ensuring high quality drinking water. Distribution systems-consisting of pipes, pumps, valves, storage tanks, reservoirs, meters, fittings, and other hydraulic appurtenances-carry drinking water from a centralized treatment plant or well supplies to consumers' taps. Spanning almost 1 million miles in the United States, distribution systems represent the vast majority of physical infrastructure for water supplies, and thus constitute the primary management challenge from both an operational and public health standpoint. Recent data on waterborne disease outbreaks suggest that distribution systems remain a source of contamination that has yet to be fully addressed. This report evaluates approaches for risk characterization and recent data, and it identifies a variety of strategies that could be considered to reduce the risks posed by water-quality deteriorating events in distribution systems. Particular attention is given to backflow events via cross connections, the potential for contamination of the distribution system during construction and repair activities, maintenance of storage facilities, and the role of premise plumbing in public health risk. The report also identifies advances in detection, monitoring and modeling, analytical methods, and research and development opportunities that will enable the water supply industry to further reduce risks associated with drinking water distribution systems.