Download or read book The Mechanism of Initiation of Protein Synthesis in E Coli written by Samuel D. Bernal and published by . This book was released on 1974 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Nicolaas Naaktgeboren and published by . This book was released on 1976 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Nicolaas Naaktgeboren and published by . This book was released on 1976 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Robert Benne and published by . This book was released on 1973 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Robert Benne and published by . This book was released on 1973 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanisms of Initiation of Protein Synthesis in Escherichia Coli written by and published by . This book was released on 1973 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Robert Benne (Genetica) and published by . This book was released on 1973 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The mechanism of initiation of protein synthesis in Escherichia coli A model for the recycling of initiation factors written by and published by . This book was released on 1973 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Initiation of Protein Synthesis in E Coli Extracts written by Daniel Kolakofsky and published by . This book was released on 1967 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Study on the Initiation of Protein Synthesis in Escherichia Coli written by Benjamin Mautner Blumberg and published by . This book was released on 1974 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Mechanism of Protein Synthesis and Its Regulation written by L. Bosch and published by . This book was released on 1972 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mechanisms of Protein Synthesis written by Engin Bermek and published by Springer. This book was released on 1985 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Model System for the Initiation of Protein Synthesis in E Coli Initiation of Polyphenylalanine Synthesis with N aceryl phe tRNA written by Edward Benson Klem and published by . This book was released on 1969 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Production of Complex Heterologous Proteins and Protein Assemblies Using E Coli based Cell free Protein Synthesis written by John Patrick Welsh and published by Stanford University. This book was released on 2011 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Swartz lab has put much effort into understanding the underlying principles of E. coli-based cell-free protein synthesis (CFPS), and the technology has developed into a scalable, affordable platform for producing a wide range of protein targets. Key breakthroughs have included activating central metabolism, stabilization of critical amino acids, controlling the redox environment to produce proteins containing disulfide bonds, and using scale-up technologies to produce proteins at milligram quantities. My work has sought to expand this CFPS technology for producing valuable and complex eukaryotic protein targets by manipulating and optimizing the folding of these proteins in the heterologous CFPS environment. Furthermore, I have sought to apply these advances to specific applications of interest. By modifying a key molecular chaperone native to the eukaryotic endoplasmic reticulum (ER), the Hsp70-family chaperone, BiP, soluble production was increased in CFPS reactions for specific proteins normally secreted through this organelle, namely those from the immunoglobulin superfamily which includes antibodies, T-cell receptors, and many membrane receptors. First, the functional properties of BiP were compared to that of the E. coli Hsp70, DnaK. A fusion protein was then constructed between BiP and the ribosome-binding portion of the E. coli protein, trigger factor, to localize BiP to translating ribosomes. This replicated the native function of BiP, which provides co-translational folding assistance to nascent polypeptides. After verifying its bioactivity, this fusion protein was utilized in CFPS reactions to indicate that the chaperone functions of BiP are specific to proteins normally secreted through the eukaryotic ER, whereas DnaK demonstrates a more general chaperone mechanism. Since the discovery that somatic cells could be reprogrammed back to a pluripotent state through the viral expression of a specific set of transcription factors, there has been great interest in reprogramming using a safer and more clinically relevant protein-based approach. Production of these transcription factor proteins was greatly increased by fusing them to the C-terminus of the solubility partner, IF2 domain 1 (IF2D1). While the fusions provided marginal benefit in molar yields using a CFPS approach, in vivo E. coli expression produced the transcription factors in soluble form. The fusion proteins could be purified in milligram quantities from liter shake-flask cultures, whereas essentially no soluble protein accumulated without the fusion partner. The transcription factor fusions bound specifically to their consensus DNA sequences and partially activated some of their downstream gene targets. Another application utilizing CFPS technology is an enhanced luciferase mutant from the marine copepod, Gaussia princeps (GLuc). GLuc is both the smallest and brightest known luciferase, and previous work from our lab demonstrated that this protein could be produced at higher volumetric yields and specific activities in CFPS compared to conventional protein expression systems. By mutating key residues in the Gaussia luciferase sequence, the luminescence half-life was shown to increase over ten-fold while maintaining the initial specific activity of the wild-type. This improved mutant provides a valuable imaging agent to use in fusions and bioconjugates with other proteins such as those that recognize cell surface markers on cancer cells. In a final application, influenza vaccines were produced using CFPS by isolating specific fragments of the protein hemagglutinin (HA), a viral surface protein. Specific mutations as well as a C-terminal trimerization domain were critical for producing this protein fragment in both its monomeric and native trimeric forms. By using the CFPS platform to incorporate non-natural amino acids (nnAAs) with alkyne functional groups, the HA proteins were covalently 'clicked' to virus-like particles (VLPs) that had surface exposed nnAAs with azide functionality. The VLPs provide an immunogenic delivery platform that efficiently traffics to lymph nodes and allows for co-attachment of other adjuvants in addition to the primary HA antigen. This vaccine platform was characterized and tested in mouse models and compared to both a standard influenza vaccine as well as free HA protein fragments. In summary, CFPS is a valuable and robust method of protein production for a variety of targets. My thesis has sought to use this platform as a means to better understand folding pathways of complex, eukaryotic proteins and improve production of these proteins. To this end, CFPS has been shown to be a valuable method for elucidating and manipulating chaperone function, producing difficult proteins with enhanced function, and as a platform to produce novel vaccines.

Download or read book Protein Synthesis and Ribosome Structure written by Knud H. Nierhaus and published by John Wiley & Sons. This book was released on 2004-10-15 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knud Nierhaus, who has studied the ribosome for more than 30 years, has assembled here the combined efforts of several scientific disciplines into a uniform picture of the largest enzyme complex found in living cells, finally resolving many decades-old questions in molecular biology. In so doing he considers virtually all aspects of ribosome structure and function -- from the molecular mechanism of different ribosomal ribozyme activities to their selective inhibition by antibiotics, from assembly of the core particle to the regulation of ribosome component synthesis. The result is a premier resource for anyone with an interest in ribosomal protein synthesis, whether in the context of molecular biology, biotechnology, pharmacology or molecular medicine.

Download or read book Temperature Dependent Initiation of Protein Synthesis in Escherichia Coli written by Hannah Friedman and published by . This book was released on 1970 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: