Download or read book The Isolation and Partial Characterization of E s c h e r i c h i a C o l i Mutants Deficient in Uracil DNA Glycosylase written by Patricia Anne Rockstroh and published by . This book was released on 1978 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Isolation and Partial Characterization of Some Mutants of Escherichia Coli K 12 with Temperature sensitive Synthesis of DNA written by Philip Louis Carl and published by . This book was released on 1968 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Isolation and Partial Characterization of Mutants of E Coli with Cold Sensitive Synthesis of DNA written by Lucy Ann Waskell and published by . This book was released on 1973 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Isolation and Characterization of a Mutant of Escherichia Coli Deficient in DNA Polymerase II written by Judith Lynn Campbell and published by . This book was released on 1974 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Partial Characterization of a Temperature sensitive Escherichia Coli Mutant with Altered Leucyl transfer Ribonucleic Acid Synthetase written by Bahram Moezzi Ghajar and published by . This book was released on 1972 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Escherichia Coli Double strand Uracil DNA Glycosylase and Analysis of Uracil initiated Base Excision DNA Repair written by Jung-Suk Sung and published by . This book was released on 2002 with total page 790 pages. Available in PDF, EPUB and Kindle. Book excerpt: Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized with respect to activity, substrate specificity, product DNA binding, and mechanism of action. Purified Dug excised both uracil and ethenocytosine specifically from double-stranded DNA substrates. One distinctive characteristic of Dug was that the purified enzyme removed a near stoichiometric amount of uracil from DNA containing U/G mispairs. The observed lack of turnover was attributed to tight binding of Dug to the apyrimidinic-site (AP) contained in the DNA reaction product. Catalytic activity was stimulated in the presence of E. coli endonuclease IV that caused AP-site incision and dissociation of Dug. By using enzyme complementation experiments, Dug was shown to initiate uracil-initiated base excision repair (BER) in E. coli (ung) cell-free extracts. The relative rate of repair of uracil- and ethenocytosine-containing DNA in isogenic E. coli cells that were proficient or deficient in Ung and/or Dug was measured using a novel competition assay. Complete ethenocytosine-initiated BER displayed an absolute requirement for Dug and occurred at the same rate as uracil-initiated BER in the presence of both Ung and Dug. However, the rate of Dug-mediated ethenocytosine-DNA repair was 8-fold faster than that of uracil-DNA mediated by Dug. The distribution of BER patch sizes associated with both uracil- and ethenocytosine-containing DNA showed similar results. In both cases, DNA repair synthesis utilized predominantly a long patch BER mechanism involving the incorporation of 2-20 nucleotides. A previously unidentified "very long patch" mechanism of BER involving the incorporation of more than 200 nucleotides was identified and shown to be mediated by DNA polymerase I. The rate-limiting step associated with uracil-initiated BER was found to involve DNA ligase and the distribution of BER patch size was modulated by the ratio of DNA polymerase I and DNA ligase. The fidelity of DNA repair synthesis associated with complete uracil-DNA BER was measured using E. coli cell-free extracts that were proficient or deficient in Ung activity and determined to be 5.5 x 10−4 and 19.7 x 10−4, respectively.

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Recombinational Repair of DNA Damage written by Andrei Kuzminov and published by Landes Bioscience. This book was released on 1996 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Journal of the National Cancer Institute written by and published by . This book was released on 1991 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.

Download or read book Progress in Nucleic Acid Research and Molecular Biology written by and published by Academic Press. This book was released on 1979-03-15 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in Nucleic Acid Research and Molecular Biology

Download or read book Abstracts of microbiology and hygiene written by and published by . This book was released on 1978 with total page 714 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics Abstracts written by and published by . This book was released on 1998 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Oxidative Damage to Nucleic Acids written by Mark D. Evans and published by Springer Science & Business Media. This book was released on 2009-06-11 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides up-to-date coverage of selected topics in nucleic acid oxidation. The topics have been selected to cover everything from basic chemical mechanisms, repair of damage and the biological and pathological meaning of DNA oxidation. The chapters are authored by leading, research active, international experts in the respective topics.

Download or read book Zentralblatt f r Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene written by and published by . This book was released on 1979 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Energy Research Abstracts written by and published by . This book was released on 1988 with total page 1684 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Processing of RNA 1983 written by David Apirion and published by CRC Press. This book was released on 2017-11-22 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a fast-moving field it is unlikely that articles written more than a year ago would be completely up to date. The purpose of this book is to bring to the nonspecialist an overall view as well as an update on the state of the art as it existed in the beginning of 1982, and to the specialist the opportunity to have a single source of information for how the other organisms do it, and also to enable him to find out the status of the various aspects of RNA processing with which he might not be to familiar. even if only some of these goals are achieved, all those who labored so diligently to bring about the publication of this book would be more than gratified.