Download or read book The Host pathogen Interaction and Breeding and Genetics of Resistance for Common Bacterial Blight of Common Bean Phaseolus Vulgaris L Caused by Xanthomonas Campestris Pv Phaseoli and X C Pv Phaseoli Var Fuscans written by Robert Wayne Duncan and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Common bacterial blight (CBB) caused by Xanthomonas campestris pv. phaseoli Smith (Dye) (Xcp, synonym: X. axonopodis pv. phaseoli [Smith] Vauterin et al.) and X. c. pv. phaseoli var. fuscans (Burkholder) Starr & Burkholder (Xcpf, synonym: X. fuscans subsp. fuscans sp. nov.) is the most important bacterial disease of common bean (Phaseolus vulgaris L.). Host resistance is the best method of management for this seed-borne disease. The first objective of this research was a host-pathogen diversity study, where new Xcp and Xcpf genotypes associated with CBB in Wisconsin were identified. These new genotypes, along with five previously reported genotypes, were inoculated onto 27 common bean genotypes having different combinations of CBB resistance QTL and markers. Eight host genotypes were identified that were resistant to all pathogen genotypes. The second objective was to compare direct disease selection (DDS) with marker-assisted selection (MAS) for the development of CBB resistant breeding lines, and the cost-effectiveness of the two selection methods. More CBB-resistant breeding lines were generated with DDS under conditions of high disease pressure in the greenhouse. However, under moderate disease pressure in the field, there were no significant differences between the two selection techniques. Under the parameters of this study, DDS was more cost-effective than MAS. Finally, the relationship between flower and seed color, CBB resistance, a CBB resistance SCAR marker (BC420) and the SOD12490 marker associated with the V pigmentation locus was investigated. In an F2 population and in the F3 progeny test, no recombination was found between colored flowers (purple [P] and lilac [L]), a dark undesirable DRK seed coat color and presence of the BC420 marker. All plants in the F2 and F3 that lacked the BC420 marker had white/lilac-striped (wl) flower color and the desirable DRK seed color. The SOD12490 marker was not linked with CBB resistance. Some plants with wl flowers were resistant but lacked the BC420 marker, indicating unidentified QTL in the CBB-resistant donor germplasm (VAX 3, Wilkinson 2, or I9365-25). Together, this information will facilitate the improvement of CBB resistance in all market classes of common bean.
Download or read book Transcriptome Analysis of Common Bean Phaselous Vulgaris in Response to Infection by Bacterial Bight Pathogen Xanthomonas Axonopodis Pv Phaseoli written by Laura Cox and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the most devastating diseases affecting common bean (Phaseolus vulgaris L.) production is common bacterial blight (CBB) caused by the bacterium Xanthomonas axonopodis pv. phaseoli (Xap). Breeding for resistant cultivars represents the best strategy for CBB immunity, but the molecular mechanisms underlying the interaction between P. vulgaris and CBB are little understood. A transcriptome analysis was undertaken to elucidate this plant-pathogen interaction in a CBB-resistant recombinant inbred line (RIL) and a CBB-susceptible RIL. A total of 800 differentially expressed genes were identified following Xap inoculation, with the majority upregulated in the CBB-resistant RIL 48 hours after inoculation. Upregulated genes included isoflavonoid biosynthesis genes. A metabolite analysis revealed that the isoflavonoids daidzein and genistein, and the phytoalexins coumestrol and phaseollinisoflavan accumulated in the CBB-resistant RIL. These findings are important for targeted breeding approaches aiming to enrich these phytoalexins in P. vulgaris as a strategy to enhance resistance to CBB.
Download or read book Host pathogen Interaction of the Tepary Bean Phaseolus Acutifolius with the Common Bean Blight Pathogen Xanthomonas Campestris PV phaseoli written by Mildred Zapata and published by . This book was released on 1989 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Common Bacterial Blight Resistance Genes in the Resistant Common Bean Phaseolus Vulgaris Variety OAC Rex written by Denise Cooper and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Inheritance of Resistance to Common Blight Caused by Xanthomonas Campestris Pv Phaseoli Smith Dye in Phaseolus Vulgaris L Bean Line XR 235 1 1 written by Sheilachu L. Paje-Gomez and published by . This book was released on 1988 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effect of Host Genotype on Multiplication Distribution and Survival of Bean Common Blight Bacteria Xanthomonas Phaseoli written by Claudio R. Cafati and published by . This book was released on 1979 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Pathogenic Variation in Common Xanthomonas Phaseoli and Fuscous Xanthomonas Phaseoli Var Fuscans Bacterial Blights of Bean Phaseolus Vulgaris L written by Ephraim J. A. Ekpo and published by . This book was released on 1975 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book EVALUATION OF COMMON BACTERIAL BLIGHT RESISTANCE IN A RESISTANT INTER CROSS POPULATION OF COMMON BEAN written by Kelli M. Durham and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacterial Blights of Common Bean written by Michael Lennon O'Leary and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Common bean is a genetically diverse crop that is a staple food in many regions of the world. However, common bean is affected by numerous diseases that can negatively impact production. There are three major bacterial diseases of common bean: halo blight, caused by Pseudomonas syringae pv. phaseolicola (Psp), bacterial brown spot, caused by P. syringae pv. syringae (Pss), and common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Xap) and X. fuscans subsp. fuscans (Xff). Disease symptoms caused by Psp and Pss can be difficult to distinguish in certain common bean genotypes and under certain environmental conditions. Precise pathogen identification is important in terms of potential yield loss and the need for disease management. Symptoms with characteristics of both halo blight and bacterial brown spot were observed on a newly developed cultivar of dark red kidney beans in Wisconsin, and pseudomonads were recovered from all samples. To determine the etiology of this disease, a method of identification was developed that involved 1) PCR with primers specific for toxin genes utilized by Psp and Pss, 2) the ability to induce a hypersensitivity response in tobacco leaves, and 3) symptoms induced on common bean. When applied to the unusual disease phenotype on this dark red kidney variety most of these were atypical symptoms of halo blight. This method was then successfully applied to identify bacterial brown spot in disease samples from Oregon, and halo blight and bacterial brown spot in disease samples from Turkey. Xap and Xff strains induce identical symptoms on common bean but are genetically distinct species. Strains of Xap and Xff can be differentiated in culture by the production of a brown diffusible pigment by Xff strains. However, some Xap strains, particularly those that originate from East Africa, are more genetically related to Xff strains than to the globally predominant Xap strains, and show pathogenic variability on certain cultivars, e.g., Andean versus Middle American. To better understand the genetic diversity among Xap and Xff strains, the complete genome sequences of three globally predominant Xap strains, two East African Xap strains, three Xff strains, and one Xanthomonas spp. strain that is associated with common bean but not pathogenic were determined using long-read sequencing technology. Whole-genome alignment revealed differences in chromosome organization between Xap strains, East African Xap strains, and Xff strains; additionally, mobile element content is variable among these strains. Conserved genetic factors, e.g. conserved plasmids and type III secreted effector proteins, some of which may have been horizontally acquired by Xap and Xff strains, were identified. The brown diffusible pigment produced by Xff strains is the result of an oxidation product of homogentisic acid, which is referred to as pyomelanin. In the tyrosine catabolism pathway, homogentisic acid is produced by 4-hydroxyphenylpyruvate dioxygenase (HPPD) and converted to 4-maleylacetoacetate by homogentisate dioxygenase (HmgA). The complete genome sequences of Xap and Xff strains revealed that Xff strains have a conserved mutation in the hmgA gene, whereas Xap strains have an intact hmgA gene. HPPD deletion mutants in an Xff strain resulted in loss of pyomelanin (brown pigment) production, whereas deletion of hmgA in two Xap strains resulted in brown pigment production, making Xap colonies resemble those of wild-type Xff strains. Additionally, complementing a wild-type Xff strain with a functional hmgA gene eliminated visible pyomelanin accumulation. Finally, virulence of a Xff HPPD and a Xap hmgA deletion mutant was assessed on twelve bean cultivars, including both Andean and Middle American gene pools, with different levels of disease resistance to common bacterial blight. There was no significant differences in virulence between brown pigment mutants and wild-type strains.
Download or read book Breeding Dry Beans Phaseolus Vulgaris L for Common Bacterial Blight Resistance Derived from Phaseolus Acutifolius A Gray written by Jeffrey Bryant McElroy and published by . This book was released on 1985 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Use of Marker assisted Selection to Breed for Resistance to Common Bacterial Blight in Common Bean written by Patrick Daniel O'Boyle and published by . This book was released on 2004 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Development of a Niemann Pick Gene based Marker in Common Bean Phaseolus Vulgaris L for the Selection of Common Bacterial Blight Resistance written by Emily Morneau and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Common bacterial blight (CBB) is a major disease in common bean (Phaseolus vulgaris) caused by Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans. By characterizing alleles in three candidate genes within white bean line OAC Rex, a codominant molecular marker for resistance was created. The Niemann-Pick polymorphism (NPP) marker was developed to screen common beans for CBB resistance, and is anchored in a candidate resistance gene (Niemann-Pick like gene). Individuals in two segregating populations were screened for allelic state and disease susceptibility. It was found that the NPP marker is significantly more efficient (8% decrease in error rate) in comparison to other markers. Within the OAC Rex pedigree, the resistant allele for the NPP marker was found solely within progeny from the introgression of Phaseolus acutifolius into P. vulgaris. Lastly, the two additional candidate resistance genes investigated (R-genes 231733-8-004 and 231733-8-005), were found to have no exploitable polymorphisms.
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1996 with total page 2368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Xanthomonas written by Jean Swings and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Xanthomonas is a bacterial plant pathogen which infects a wide range of crops worldwide. This book presents an overview of the host plants and the diseases caused by the pathogen on different crops.
Download or read book Pathogen and Microbial Contamination Management in Micropropagation written by Alan C. Cassells and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo.
Download or read book The Common Bean Genome written by Marcelino Pérez de la Vega and published by Springer. This book was released on 2017-12-20 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides insights into the genetics and the latest advances in genomics research on the common bean, offering a timely overview of topics that are pertinent for future developments in legume genomics. The common bean (Phaseolus vulgaris L.) is the most important grain legume crop for food consumption worldwide, as well as a model for legume research, and the availability of the genome sequence has completely changed the paradigm of the ongoing research on the species. Key topics covered include the numerous genetic and genomic resources, available tools, the identified genes and quantitative trait locus (QTL) identified, and there is a particular emphasis on domestication. It is a valuable resource for students and researchers interested in the genetics and genomics of the common bean and legumes in general.
Download or read book Microbial Plant Pathogens Detection and Disease Diagnosis written by P. Narayanasamy and published by Springer Science & Business Media. This book was released on 2010-10-20 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial plant pathogens causing qualitative and quantitative losses in all corps are present not only in the infected plants, but also in the environmental comprising of soil, water and air. The vectors present in the environment spread the microbial pathogens to short and/or long distances. Detection of microbial pathogens rapidly and reliably by employing suitable sensitive applicable for different ecosystems. The pathogens have to be identified precisely and differentiated and quantified to plan appropriate short- and long-term strategies to contain the incidence and spread of diseases induced by them. This book aims to present all relevant and latest information on the detection techniques based on the biological, biochemical, immunological and nucleic acid characteristics of microbial pathogens presents in the host plants, as well as in the natural substrates that support the survival and perpetuation of the pathogens.
