Download or read book The Glycoconjugates V2 written by Martin Horowitz and published by Elsevier. This book was released on 2012-12-02 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Glycoconjugates: Mammalian Glycoproteins, Glycolipids, and Proteoglycans Volume II is a collaboration of different experts in the field of molecular biology on the subject of glycoconjugates. The text of the second volume covers topics such as the structure and biosynthesis of connective tissue proteoglycans, glycoproteins, and glycolipids; and glycoprotein and glycolipid catabolism and degradation. It also discusses the structure, composition, and isolation of surface membranes; and lectins – its immunological aspects and its use in the study of mammalian glycoprotein. The book is recommended for molecular biologists, organic chemists, and biochemists who would like to know more about glycolipids and glycoproteins and their applications.
Download or read book Carbohydrate based Vaccines written by René Roy and published by ACS Symposium. This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first of its kind entirely dedicated to carbohydrate vaccines written by renowned scientists with expertise in carbohydrate chemistry and immunochemistry. It covers the synthesis of carbohydrate antigens related to bacteria and parasites such as: Heamophilus influenza, Streptococcus pnemoniae, Shigella flexneri, Candida albicans, Mycobacterium tuberculosis, and Chlamydia. The first three chapters are of wide interest as they cover fundamental concerns in new vaccine developments. The first one presents the immune system and how carbohydrate antigens are processed before protective antibodies are produced. It also illustrates antigen presentation in the context of major histocompatibility complexes (MHCs). The second chapter describes regulatory issues when carbohydrate vaccines are involved while the third one discuss several techniques used in conjugation chemistry and the implication of certain chemical linkages that may induce unexpected anti-linker antibodies. This section will be particularly appealing for those involved in drug-conjugate design, pro-drug developments, and drug vectorization. The book concludes with one chapter that illustrates the principle through which peptide antigens can functionally mimic carbohydrate epitopes, thus, unraveling the potential for peptide surrogates as replacement for complex carbohydrate structures. This book is unique in that it covers all aspects related to carbohydrate vaccines including the success story with the first semi-synthetic bacterial polysaccharide vaccine against Heamophilus influenza type b responsible for pneumonia and meningitis, liable for more than 600,000 infant deaths worldwide in developing countries. The book also presents regulatory issues and will thus be vital for government agencies approving candidate vaccines. It widely covers synthetic methodologies for the attachment of carbohydrate antigens to peptides and immunogenic protein carriers. Vaccines against bacterial antigens, cancer, and parasites are also discussed by worldwide experts in this field in details. No other book contains such a wide panel of different expertise. It will also be useful to students and researchers involved with the immunology of forreings antigens and how the under appreciated carbohydrate antigens are processed by the immune system.
Download or read book Transforming Glycoscience written by National Research Council and published by National Academies Press. This book was released on 2012-10-23 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new focus on glycoscience, a field that explores the structures and functions of sugars, promises great advances in areas as diverse as medicine, energy generation, and materials science, this report finds. Glycans-also known as carbohydrates, saccharides, or simply as sugars-play central roles in many biological processes and have properties useful in an array of applications. However, glycans have received little attention from the research community due to a lack of tools to probe their often complex structures and properties. Transforming Glycoscience: A Roadmap for the Future presents a roadmap for transforming glycoscience from a field dominated by specialists to a widely studied and integrated discipline, which could lead to a more complete understanding of glycans and help solve key challenges in diverse fields.
Download or read book A Laboratory Guide to Glycoconjugate Analysis written by P. Jackson and published by Birkhäuser. This book was released on 2012-12-06 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: 18. 2 Principle of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. 3 Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 Principle of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. 3 Determination of Association Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. 4 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 General considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. 5 Limitations of the Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. 3 Sample Detection and Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Sample detection by blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. 4 Analysis of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring sample mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical analysis of data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 Interpreting ACE patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 Reverse ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII List of Contributors Nebojsa Avdalovic John T. Gallagher Dionex Corporation Cancer Research Campaign Department of Medical Oncology 445 Lakeside Drive University of Manchester Sunnyvale, CA 94086 Christie CRC Research Centre Klaus Biemann Wilmslow Road Department of Chemistry Manchester M20 4BX Massachusetts Institute of Technology UK Cambridge, MA 02139-4307 USA Geoffrey R.
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Carbohydrate Analysis by Modern Liquid Phase Separation Techniques written by Ziad El Rassi and published by Elsevier. This book was released on 2021-08-15 with total page 956 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1995 with total page 1556 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ultracytochemistry of Intracellular Membrane Glycoconjugates written by Wolfram F. Neiss and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: This investigation is concerned with the ultracytochemistry of glycoconjugates - i. e., the carbohydrate moieties of glycoproteins and glycolipids - attached to intracellular post-Golgi membranes (membranes of lysosomes, peroxisomes, secretory granules of exocrine and endocrine gland cells). In addition mitochon dria have been studied. There are at present very few cytochemical studies, none of them systematic, on intracellular membrane-bound glycoconjugates. So far it has only been re ported that phosphotungstic acid (PTA) at low pH (pH
Download or read book Glycoconjugate Research written by John Gregory and published by Elsevier. This book was released on 2012-12-02 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glycoconjugate Research, Volume II contains the proceedings of the Fourth International Symposium on Glycoconjugates held in Woods Hole, Massachusetts in 1977. The papers explore the biosynthesis and regulation of glycoconjugates, particularly those of cell membranes, and glycolipid storage diseases. This volume is comprised of 95 chapters divided into three sections. After reviewing the regulatory mechanisms underlying glycoprotein synthesis, it turns to enzymic modifications of sialic acids in the course of glycoconjugate biosynthesis. The following chapters focus on the biosynthesis and characterization of lipid-linked sugars in the outer membrane of liver mitochondria; effect of bacitracin on the biosynthesis of dolichol derivatives in calf pancreas microsomes; secretion of proteoglycans by chondrocytes; and heterogeneity of arterial proteoglycans. The biosynthesis of elastin by chondroblasts in monolayer cultures is also considered, along with the phosphorylation of proteoglycans in human articular cartilage. The final chapter describes a double-antibody radioimmunoassay for soluble and cell-surface blood group Ii antigens. This book will be a useful resource for biochemists.
Download or read book Enzymes of Lipid Metabolism II written by Louis Freysz and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the proceedings of the 2nd meeting on "Enzymes of Lipid Metabolism" which took place in Strasbourg in October, 1985. It is a sequel to the first conference bearing this title which took place, also in the vicinity of Strasbourg, in March, 1977. In either case the meetings were coorganized by L. Freysz of Strasbourg, France and S. Gatt of Jerusalem, Israel. The present meeting was set up as a joint NATO Advanced Research Workshop and CNRS-INSERM International Symposium. The conference was guided by two principles, namely, that science has no bounderies, neither has the study of lipid metabolism. Participants came from Europe, the USA, Israel and Japan and represented areas of research in lipid metabolism involving fatty acid s, cholesterylesters, glycero-and sphingolipids. The experimental approaches utilized purified enzymes, artificial and biological membranes, as well as a variety of cells, primary or cultured lines. A session was also devoted to modification of lipid enzymes and metabolism resulting from inherited, inborn defects such as the lipid storage diseases which are caused by genetic modification of degradative enzymes of lipid metabolism. A second type of disease stemming from a defect in a cell organnel (ie, the peroxisome) was also discussed. The eight and one and a half years which elapsed since the previous meeting, highlighted the changing emphasis of research in. lipid metabolism.
Download or read book Glycoconjugates written by Howard J. Allen and published by CRC Press. This book was released on 1992-04-15 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glycoconjugates Composition: Structure, and Function provides an excellent overview of the composition, biosynthesis, function and structure of the carbohydrate chains of glycoconjugates from higher organisms. It is recommended as a core reference text, providing excellent coverage of the glycoconjugate field.
Download or read book Sialobiology Structure Biosynthesis and Function Sialic Acid Glycoconjugates in Health and Disease written by Joe Tiralongo and published by Bentham Science Publishers. This book was released on 2013-02-13 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: This ebook presents a summary of central aspects of sialobiology (i.e., the study of sialic acid and its relevance to biology). The importance of substitution by the sugar sialic acid and the role played by sialylated structures (eg. glycoproteins, glycolipids, glycoconjugates) in immune recognition, neural cell growth, embryogenesis and disease development including microbial pathogenesis and cancer progression, has become well-established. Since 1995, the field of sialobiology has expanded greatly as many of the key enzymes involved in sialic acid biosynthesis, as well as the vast majority of sialic acid binding lectins involved in immune recognition, have only been cloned, characterised and structural eluciated after the publication of earlier works on the subject. This e-book also covers these recent developments. Chapters in this e-book have been contributed by eminent sialobiologists. Therefore, a book of this nature is timely and will prove to be a definitive volume with a high impact in this field for glycobiologists and cell biologists.
Download or read book Cell Surface and Extracellular Glycoconjugates written by and published by Academic Press. This book was released on 2012-12-02 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book serves as a much-needed guide for new investigators in the field of glycobiology as well as researchers in other disciplines attempting to define the role of glycoconjugates in their biological systems. Cell Surface and Extracellular Glycoconjugates: Structure and Function describes the determination of structure and function of glycoconjugates that occur on the cell surface and in molecules involved in cell-matrix interactions. The broad scope of thebook covers basic background and technical information in this rapidly expanding field. The text is specifically designed to inform researchers in diverse disciplines of the approaches and resources that can be employed to address problems in glycoconjugate biology. - Structure of cell surface carbohydrates - Biosynthesis of glycoproteins - Biological signals associated with glycoproteins - Diversity of glycoprotein structure
Download or read book The Molecular Immunology of Complex Carbohydrates 2 written by Albert M. Wu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chang-Gung Univ., Tay-yuan, Taiwan. Proceedings of the 15th International Glycoconjugate Conference held August 28 to September 2, 1999, in Taiwan.
Download or read book Understanding PTMs in Neurodegenerative Diseases written by Victor Corasolla Carregari and published by Springer Nature. This book was released on 2022-08-27 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, a part of the Proteomics, Metabolomics, Interactomics and Systems Biology series, will explain how proteomic studies of post-translational modifications (PTMs) can be applied to neurodegenerative diseases and relevant studies. The goal of the book is to increase awareness among researchers about how PTMs may be helpful in understanding mechanisms in various neurodegenerative diseases through proteomic studies. This book will serve as a tool for those who want to begin work in the proteomics field and explore how to implement PTMs studies into their work. Chapter authors will describe different PTMs enrichment methods developed by experts in the field so that researchers may learn to apply these methods and techniques to new studies. Divided into three sections, chapters will cover sample preparation, data quality, enrichment techniques, guidelines on how to analyze PTMs, and explain the role of PTMs and different brain diseases. Among those topics includes will be brain cancer, SLA disease, Parkinsons disease, muscular dystrophies, and schizophrenia. This volume will be useful for researchers and students studying brain and neurodegenerative diseases who are interested in delving into work with proteomic studies and PTMs.
Download or read book Recent Progress in Pharmaceutical Nanobiotechnology A Medical Perspective written by Habibe Yılmaz and published by Bentham Science Publishers. This book was released on 2023-12-28 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent Progress in Pharmaceutical Nanobiotechnology: A Medical Perspective offers a comprehensive exploration of the dynamic field of pharmaceutical nanobiotechnology, focusing on its medical applications. This edited reference serves as a valuable resource for researchers, students, and professionals in various disciplines (pharmacology, biotechnology, clinical medicine and nanotechnology) , providing insights into the latest advancements and practical implications of nanotechnology in the pharmaceutical sector. The book presents 14 edited and referenced chapters that cover several themes for readers. General Pharmaceutical Nanobiotechnology: Introduction to the interdisciplinary field Exploration of nanoscale materials for medical purposes Nanoparticle Development and Applications: Bioinspired Nanomedicines Lipid-Based Nanocarriers Metallic Nanoparticles and Their Applications Nanoparticle Targeting Strategies Nanomedicine-Based Therapies for Cancer Stem Cells Biotechnological Aspects: Biotechnological Significance of Exosomes Glycoconjugates: Biosynthesis and Functions Innovative Nanotherapies: Novel Nanotechnological Approaches for Glioblastoma Biocompatibility of Nanomedicines and Bio Corona Diagnostic and Sensing Applications: Role of Nanoparticular/Nano Vesicular Systems as Biosensors In Vitro Applications of Drug-Carrying Nanoparticles in Cell Culture Studies In Vivo Imaging Techniques: Bioluminescence and Fluorescence Imaging Precision Medicine: The Role of Nano and Biopharmaceutics in Precision Medicine Audience Postgraduate researchers in pharmaceutical biotechnology; pharmacy professionals and academicians
Download or read book Plant Lectins written by A. Pusztai and published by Cambridge University Press. This book was released on 1991 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume surveys the chemistry, biochemistry, biosynthesis, metabolism and pharmacological properties of lectins. Lectins, which are most commonly found in plants, are widespread natural products with striking biological activities. Their specific ability to recognise and bind to simple or complex saccharides facilitates their role as effective information protein molecules. As agents of cell-to-cell recognition, lectins promote symbiosis between plants and specific nitrogen-fixing soil bacteria. As natural defensive molecules, they can protect plants against predators such as bacteria, fungi and insects. As part of our diet, lectins are powerful exogenous growth factors in the small intestine and influence our health, the digestive function and the bacterial ecology of the alimentary tract. Lectins are also important research tools in preparative biochemistry and cell science.
