Download or read book The Functional Role of Herpes Simplex Virus Type 1 HSV 1 Glycoproteins in Induction of Cell Fusion written by Ali Saharkhiz-Langroodi and published by . This book was released on 1996 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Structural and Functional Analysis of Herpes Simplex Virus Type 1 Glycoprotein L an Essential Component of the Viral Fusogenic Complex written by Michael J. Novotny and published by . This book was released on 1996 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Herpes Simplex Virus Type I HSV 1 induced Cell Fusion written by Tracy L. Terry-Allison and published by . This book was released on 1999 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book The Role of Herpes Simplex Virus Type 1 Glycoprotein L in Virus Entry and Cell Fusion written by Yuri M. Klyachkin and published by . This book was released on 2008 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Role of Cell Surface Heparan Sulfate in the Binding and Entry of Herpes Simplex Virus and in Virus induced Cell Fusion written by Mei-Tsu Shieh and published by . This book was released on 1992 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Exploring the Functional Role of Herpes Simplex Virus Type 1 Glycoprotein H in Virus Entry written by Amy Yuan Zhou and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Biology of Herpes Viruses written by Klaus Osterrieder and published by Springer. This book was released on 2017-05-20 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes viruses are widely distributed in nature, causing disease in organisms as diverse as bivalves and primates, including humans. Each virus appears to have established a long-standing relationship with its host, and the viruses have the ability to manipulate and control the metabolism of host cells, as well as innate and adaptive antiviral immune responses. Herpes viruses maintain themselves within hosts in a latent state resulting in virus persistence for years – usually for the life span of the hosts. Herpes viruses comprise a large number of pathogens with diverse cellular targets and biological consequences of infection. What they have in common is their structure and the fact that they establish a dormant (latent) infection in their hosts that usually persists for life. The reviews here will highlight the general principles of herpes virus infection, with equal attention to overall principle and important difference. Also, the cell type- and life-style dependent differences in the establishment and maintenance of virus persistence will be covered.
Download or read book Herpes Simplex Virus New Insights for the Healthcare Professional 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Herpes Simplex Virus. The editors have built Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Herpes Simplex Virus in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Viral Fusion Mechanisms written by Joseph Bentz and published by CRC Press. This book was released on 1992-11-30 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viral Fusion Mechanisms presents the first comprehensive review on this exciting topic. The book focuses on molecular mechanisms rather than phenomonology and examines a wide range of viruses, including influenza, HIV, Sendai, SFV, Vaccinia, VSV, and RSV. Recent theoretical work on dissecting protein-mediated membrane fusion is discussed, and the most promising new technologies for elucidating mechanisms are highlighted. Viral Fusion Mechanisms is an essential reference for biophysicists, cell biologists, colloid chemists, immunologists, microbiologists, molecular biologists, and virologists.
Download or read book Human Herpesviruses written by Yasushi Kawaguchi and published by Springer. This book was released on 2018-06-12 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces and reviews several topics for each human herpesvirus. One of the most important features of the book is that it covers aspects of both basic research and clinical medicine. Herpesviridae, a family of double-strand DNA viruses, has unique biological features by which these viruses establish latency after primary infection and reactivate in later life. Nine human herpesviruses are known so far, and each of them causes a variety of diseases in both primary infection and reactivation. Since the discovery of each human herpesvirus, an abundance of findings related to them has accumulated in basic research and clinical medicine. However, the vast majority of biological features is still masked in mystery. Furthermore, a strategy of treatment and prevention has not yet been established for most human herpesviruses. A wide range of readers will be interested in this volume with its treatment of problematic points and latest findings in the field.
Download or read book Glycoprotein M and ESCRT in Herpes Simplex Virus Type 1 Assembly written by Yudan Ren and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus type 1 (HSV-1) has a large linear double-stranded DNA genome in an icosahedral capsid shell, a cell-derived lipid envelope and a proteinaceous tegument layer. There are over fifty viral proteins and many host proteins identified in HSV-1 virions. The final formation of mature virus particles requires the membrane wrapping of tegumented capsids in the cytoplasm, a process termed secondary envelopment. This process involves the coordination of numerous viral and cellular proteins and results in double-membrane structures with enveloped virions contained within cellular vesicles. Mature viruses are then released through the fusion of these virion-containing vesicles and plasma membranes. This thesis describes investigation into the functions of viral glycoprotein M (gM) and the cellular Endosomal Sorting Complexes Required for Transport (ESCRT) in secondary envelopment. Firstly, it has been reported that gH/L can be efficiently internalised and targeted to the TGN by the co-expression of gM in transfection assays. In order to examine the role of gM in guiding the localisation of viral proteins in infected cells, a HSV-1 gM deletion virus (∆gM), and its revertant virus were constructed. The major phenotype demonstrated was that the absence of gM caused the internalisation of cell surface gH/L to be inhibited and higher levels of gH/L to be observed on the cell surface. Further, lower levels of gH/L were detected in purified ∆gM virions, which was in agreement with the delayed entry kinetics, smaller plaque sizes and greater replication deficits at low multiplicity of infection observed in ∆gM infected cells. Over all the results presented in this thesis demonstrate that in infected cells the efficient incorporation of gH/L into virions relies on the function of gM in HSV-1. Secondly, during HSV-1 secondary envelopment the budding and scission of the viral envelope from the host membrane share topological similarities with the formation of intraluminal vesicle in multivesicular bodies, retrovirus budding, and abscission at the end of cytokinesis, processes that require the cellular ESCRT machinery. There are four multiprotein ESCRT complexes and many associated proteins involved in their regulation. It has been previously shown that the ESCRT-III complex and a functional ATPase VPS4 are required for HSV-1 secondary envelopment, but different from the strategy utilised by HIV-1, the recruitment of ESCRT during HSV-1 infection is independent of TSG101 and/or ALIX. Data presented in this thesis demonstrate that CHMP4A/B/C proteins of the ESCRT-III complex are specifically crucial for HSV-1 secondary envelopment. Simultaneous depletion of CHMP4A/B/C proteins significantly inhibited HSV-1 replication. Ultrastructure analysis revealed that there were virtually no extracellular virions in CHMP4A/B/C depleted samples while more free capsids were observed in the cytoplasm, although the nuclear capsids and primary envelopment events appeared to be normal. In order to identify interactions between HSV-1 and ESCRT proteins, 22 HSV-1 tegument proteins were cloned and tested against a panel of ESCRT and ESCRT-associated proteins in yeast two-hydrid assays. Analysis of positive hits from yeast two-hybrid interaction screens using GST pull-down, co-immunoprecipitation and protein co-localisation assays have validated interactions of pUL47 with CC2D1A/1B, CIN85, CHMP6 and ALIX, pUL46 and pUL49 with CC2D1A/1B and CIN85, and pUL16 with CC2D1A/1B. Furthermore, the newly identified ESCRT associated proteins CC2D1A and CC2D1B have been detected in purified virions. The role of the identified ESCRT proteins in HSV-1 replication has been investigated using siRNA depletion. Unfortunately siRNA depletions of the various ESCRT candidates individually or in combinations did not show any significant effect on HSV-1 replication. Overall these data suggest that unlike HIV and other retroviruses, HSV-1 has evolved multiple parallel pathways to hijack the ESCRT machinery to facilitate its replication, particularly, through the interactions that lead directly to the recruitment of CHMP4A/B/C proteins. Disruption of some of these pathways did not prevent HSV-1 replication in tissue culture, suggesting any one potential pathway is sufficient for ESCRT recruitment to sites of HSV-1 assembly.
Download or read book Newly Identified Roles of Glycoprotein C in Herpes Simplex Virus Infectivity written by Tri Komala Sari and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus (HSV) infections are mainly asymptomatic, but are ubiquitous in the adult population, and can be fatal in individuals with suppressed immune systems. Prophylactic and treatment measures have not been sufficient to stop disease dissemination. HSV expresses at least 12 different glycoproteins and utilizes multiple entry pathways to infect cells. This combined with its ability to establish latent infection and evade immune responses have contributed to the challenges in developing effective control measures. HSV uses the concerted action of essential glycoproteins B, D, and H/L to execute entry. HSV carries more than a dozen surface glycoproteins, and this may pose evolutionary advantages. It is likely that HSV uses specific surface glycoprotein(s) to effectively enter one pathway, but not the other. Here we report novel roles of glycoprotein C in HSV infectivity. gB from HSV-1 fully deleted for the gC gene undergoes low-pH induced antigenic changes at a pH that is 0.4-0.6 units lower than wild type. Moreover, gC-null mutant HSV exits cellular endosomes at time points 10-20 min later than wild type, suggesting that gB present in gC-null HSV fuses with a more acidic (later) endosome. We also report for the first time, low-pH antigenic changes in domain II of HSV gB. Although gC plays a role in gB antigenic changes, its absence does not affect gB oligomeric changes nor reversibility of conformational changes under conditions tested. The threshold for reversibility of gB conformational changes in wild type and gC-null HSV is shown to be around pH 5.6. This suggests that at this pH gB exists at an equilibrium of pre- and post-fusion forms. We also tested the ability of gB antibodies to block HSV infectivity in the absence of gC. We found that gC-null HSV is significantly more sensitive to neutralization by gB antibodies. The level and composition of HSV glycoproteins in wild type and gC-null mutant are similar. Interestingly, reactivity of gB antibodies with gC-null virus is greater than wild type. Control gE-null HSV is not more sensitive to neutralization by gB antibodies. Together our results demonstrate newly identified functional interactions between HSV gC and gB.
Download or read book Identification of Regions in Herpes Simplex Virus Type 1 Glycoprotein D Critical for Receptor Binding and Membrane Fusion written by Cheryl Rosemarie Jogger and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability of HSV to induce membrane fusion is critical to its infection process. First, in order to gain entry into a cell, HSV must fuse its virion envelope with the cell plasma membrane. Second, as an effective means of promoting viral spread, HSV can induce fusion of infected cells with neighboring uninfected cells. Both fusion events are dependent on the binding of gD to an entry receptor, such as HVEM or nectin-1, and on the combined activities of viral gB, gD, gH and gL. The overall goal of this study was to define regions of HSV-1 gD critical for physical and functional interactions with each of the known HSV entry receptors, including nectin-2 and 3-O-S heparan sulfate. Extensive mutational analysis was performed to identify mutations in gD that alter its receptor usage.
Download or read book Genetics and Functions of Herpes Simplex Virus Type 1 Membrane Proteins in Virus induced Cell Fusion Virion Morphogenesis and Egress written by Jeffrey M. Melancon and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Low PH triggered Conformational Changes in the Herpes Simplex Virus Glycoprotein B written by Stephen Dollery and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpesviruses can enter host cells by pH-dependent endocytic pathways in a cell-specific manner. The role of pH in herpesvirus endocytosis is unclear. Herpes simplex virus (HSV) is a paradigm for virus membrane fusion via a complex of glycoproteins. HSV glycoproteins B, D and the heterodimer H-L are necessary and sufficient for membrane fusion. This work analyzes the structure and function of HSV glycoproteins B, D, and H-L at neutral pH, and at the physiological low-pH encountered during endocytic entry. It is demonstrated that mildly acidic low pH triggers specific conformational changes in HSV gB at a pH of 5.7 to 6.0. The antigenic structure of gB functional region I that is critical for fusion is specifically altered by mildly acidic pH both in vitro and during entry into host cells. Point mutations within gB functional region 1 that block membrane fusion still allow conformational changes in region 1. This suggests that specific hydrophobic residues are essential for fusion domain insertion into the host cell membrane but not conformational change. The detected conformational changes were reversible, similar to other class III fusion glycoproteins. Exposure to mildly acidic pH directly triggered the fusion function of HSV glycoproteins and caused gB, but not other glycoproteins, to become more hydrophobic. The oligomeric conformation of gB is altered at a similar pH range. In addition, several approaches were used to monitor gB throughout glycoprotein synthesis and maturation. It is shown that gB may cotranslationally fold and oligomerize as it is synthesized on the ribosome. As gB matures it then alters conformation and/or binding partner to form antigenically distinct populations of gB within the cell and virion. I conclude that intracellular low pH induces changes in gB conformation that, together with additional triggers such as receptor-binding, are essential for virion-cell fusion during herpesviral entry by endocytosis.
Download or read book Structure and Function of the C terminal Region of a Fusion Receptor for Herpes Simplex Virus written by Santiago López and published by . This book was released on 2007 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
