Download or read book The Functional and Structural Analysis of DNA Dependent RNA Polymerase II Subunits written by Young Ik Lee and published by . This book was released on 1984 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of Nuclear DNA dependent RNA Polymerase Subunits and Tata binding Protein from Plants written by Robert Morgan Larkin and published by . This book was released on 1996 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past several years, our laboratory has characterized genes and/or cDNAs encoding many of the Arabidopsis RNA polymerase II subunits as well as a few RNA polymerase I and III subunits. Here, an analysis of the 13.6, 14.1, 16.4, 23.7, 24.3 and 135 kDa subunits of nuclear RNA polymerases from Arabidopsis is presented. These subunits are encoded by single copy genes and are expressed in Arabidopsis suspension culture cells. Comparisons of derived amino acid sequences of these Arabidopsis RNA polymerase subunits with other species suggest that nuclear RNA polymerases are highly conserved in subunit composition. A few plant RNA polymerase subunits were found to contain unique properties relative to other eukaryotic RNA polymerase subunits. For example, the 14.1 kDa subunit contains a noncolinear arrangement of conserved sequence motifs, and the 23.7 and 24.3 kDa subunits are related to a subunit that is encoded by a single gene in yeast. Similarities in amino acid sequence between subunits in eukaryotic and E. coli RNA polymerases suggest that the eukaryotic enzymes contain a group of subunits that are related to the $\beta\sp\prime\beta(\alpha)\sb2$ structure of E. coli RNA polymerase. Using these relationships, reconstitution experiments have been initiated with recombinant subunits from all three Arabidopsis nuclear RNA polymerases and yeast RNA polymerase II. Reconstitution of ($\alpha)\sb2$-like assembly intermediates with Arabidopsis and yeast subunits was carried out as an initial step towards renaturation of RNA polymerase II from recombinant subunits. Renaturation of RNA polymerase from a minimal number of recombinant subunits will provide a powerful tool for the analysis of enzyme assembly and subunit function. Purified nuclear RNA polymerases cannot accurately initiate RNA synthesis in vitro and require a number of basal transcription factors to faithfully initiate transcription in vitro. In animal cells, the TATA-binding protein (TBP) is a common subunit in four transcription factors that are required for promoter recognition by RNA polymerase I, II and III. Little is known about basal transcription factors in plants. Studies on plant TBP were initiated for reconstituting in vitro transcription systems and establishing associations between plant TBP and transcription factor complexes. A cDNA encoding soybean TBP was isolated and found to be very similar to other plant TBPs. This cDNA was used to express soybean TBP in E. coli and antibodies were raised to the recombinant factor. These antibodies were used to follow the partial purification of TBP from different species using different strategies. Plant TBP complexes resemble yeast TBP complexes in that they are easily disrupted upon cell lysis and purification, resulting in a large amount of TBP that is not associated with other factors. Strategies were developed that allow for the isolation of multiple TBP complexes in a partially pure form.
Download or read book Structure function Analysis of the Two Largest Subunits of Escherichia Coli RNA Polymerase written by Laura Magdolna Heisler and published by . This book was released on 1994 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structure function Analysis of RNA Polymerase II written by Elisabeth Lehmann and published by . This book was released on 2010 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Polymerase III Transcription written by Robert J. White and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews and summarizes the substantial body of work that has been published on the transcription by polymerase III over the past 5 years. Progress in this field has been very rapid since 1993, and this new edition incorporates all the recent developments and offers the reader a highly detailed analysis of the current state of research on this largest and most complex of the eukaryotic RNA polymerases.
Download or read book Investigations of RNA Polymerase II Subunit Structure and Function written by Peter Alexander Kolodziej and published by . This book was released on 1991 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Macromolecular Protein Complexes II Structure and Function written by J. Robin Harris and published by Springer Nature. This book was released on 2020-01-14 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book A Functional Analysis of the RNA Polymerase II Large Subunit Carboxy terminal Domain written by Rob Chapman and published by . This book was released on 2002 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structure and Function of RNA Polymerase II Bye1 Complexes written by Kerstin Kinkelin and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Subunit Function and Structure of Eukaryotic RNA Polymerase II written by Erik Bateman and published by . This book was released on 1983 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acid Polymerases written by Katsuhiko S. Murakami and published by Springer Science & Business Media. This book was released on 2013-10-22 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerases of all varieties. In other words, this book is for polymerase fans by polymerase fans. Nucleic acid polymerases play a fundamental role in genome replication, maintenance, gene expression and regulation. Throughout evolution these enzymes have been pivotal in transforming life towards RNA self-replicating systems as well as into more stable DNA genomes. These enzymes are generally extremely efficient and accurate in RNA transcription and DNA replication and share common kinetic and structural features. How catalysis can be so amazingly fast without loss of specificity is a question that has intrigued researchers for over 60 years. Certain specialized polymerases that play a critical role in cellular metabolism are used for diverse biotechnological applications and are therefore an essential tool for research.
Download or read book Subunit Structure and Function of E Coli DNA dependent RNA Polymerase written by Eugene Chi-ho Lai and published by . This book was released on 1976 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structure Function Analysis of the RNA Polymerase III Subcomplex C17 25 and Genome Wide Distribution of RNA Polymerase II written by Anna Justyna Jasiak and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNAi written by Gregory J. Hannon and published by CSHL Press. This book was released on 2003 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.
Download or read book Structural and Functional Studies of RNA Polymerase II Passage Through Nucleosomes written by Moritz Ochmann and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription by RNA polymerase II (Pol II) is an essential process of life occurring in the context of the compact eukaryotic nucleus. While transcription elongation has been extensively studied on linear DNA in vitro, our understanding of transcription on physiologically relevant chromatinized templates, consisting of long arrays of nucleosomes, remains severely limited. Recently, several structures provided snapshots of the yeast RNA polymerase II transcribed into a single nucleosome. However, information regarding the mechanism, the required components and the dynamics of nucleosome tra...
Download or read book Structure and Function of RNA Polymerase I Subunits written by Sebastian R. Geiger and published by . This book was released on 2010 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of RNA written by Masayori Inouye and published by . This book was released on 1987 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology of RNA: New Perspectives provides an overview of the developments in RNA research as well as the approaches, strategies, and methodologies used. Most of the contributing authors in the present volume participated in the Fifth Stony Brook Symposium entitled ""New Perspectives on the Molecular Biology of RNA"" in May 1986. The text is organized into six parts. Part I contains papers dealing with RNA as an enzyme. Part II presents studies on RNA splicing. Part III examines RNA viruses while Part IV focuses on the role of RNA in DNA replication. Part V is devoted to the structure ...
