Download or read book The Feasibility of Single Molecule Detection by Laser Induced Fluorescence Microscopy written by Loucinda C. Carey and published by . This book was released on 2001 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of a Single Molecule in a Capillary by Laser induced Fluorescence written by Yuan-Hsiang Lee and published by . This book was released on 1994 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Approaching Single Molecule Detection by Laser induced Fluorescence of Flowing Dye Solutions in a Capillary written by Steven John Lehotay and published by . This book was released on 1992 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Approach to Single molecule Detection by Laser induced Fluorescence written by and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A sheath flow cuvette was evaluated in laser-induced fluorescence determination of aqueous rhodamine 6G. A detection limit of 18 attograms was obtained within a one-second signal integration time. The concentration detection limit was 8.9 x 10−14 mole per liter. An average of one-half rhodamine 6G molecule was present within the 11 pL excitation volume. However, during the signal integration time a total of 22,000 analyte molecules passed through the excitation in a 0.42 microliter volume.
Download or read book Prospects for Single molecule Detection in Liquids by Laser induced Fluorescence written by and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A laser-induced fluoresence determination of aqueous solutions of rhodamine 6G resulted in a detection limit of 18 attograms, or 22,000 molecules, of rhodamine 6G. These results allow the projection to single-molecule detection with reasonable improvements in the experimental apparatus.
Download or read book Applications of Capillary Electrophoresis and Laser induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Capillary Electrophoresis and Laser induced Fluorescence Detection to the Analysis of Trace Species written by and published by . This book was released on 1995 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Ph. D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.
Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.
Download or read book Microsystem Technology written by Hans-Peter Saluz and published by Birkhäuser. This book was released on 2012-12-06 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomolecular studies are the trial of Man to understand how Nature manages information at the molecular level. The understanding of molecular informa tion handling in nature is essential for the molecular optimization in chem istry, molecular biology, molecular pharmacology and therefore - as an ex ample - for the development of specifically acting drugs. The famous recent method of technical information management is digital electronics. Over the past few years, evidence has arisen that computerized and molecular information managements have many similar and overlapping aspects. For example, both technology and nature use digitized information and both use small structures for the efficient handling of information. Furthermore, they optimize their processes in order to gain a maximum of information with a minimum of invested energy. During the last two decades, novel experimental techniques in biomolec ular sciences have paved the way for artificial biomolecular optimization. In the same time interval, the progress of micro system technology has been extended from the field of digital electronics and sensing to micro liquid hand ling, and the field of chip-supported substance handling began. It appears that the "marriage" of physical micro technology and molecular processing will be consummated soon. The contact of both fields has been realized in for ex ample DNA chips. Such connections will also become relevant in additional fields in the near future. Biomolecular investigations are the first to profit from these fast growing scientific and technical connections between micro systems and molecular sciences.
Download or read book Rapid Screening and Isolation of Single Molecules by Laser induced Fluorescence Spectroscopy in Microdroplets written by Katie Hua Heredia and published by . This book was released on 2008 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.
Download or read book Investigation of the Feasibility of Using Laser Induced Fluorescence for Concentration Measurements by Diatomic Sulfur written by William D. Harris (Jr.) and published by . This book was released on 1982 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The feasibility of using laser induced fluorescence to measure total S2 number densities in a laboratory environment is studied. A rate equation analysis modeling transitions within the B to the 3rd power negative summation u-to the 3rd power negative summation g system of S2 is developed and applied to model the frequency prime = 9, frequency double prime = 6 transition at 3214.6 angstroms. Excitation spectra were obtained over the range of 2890 to 2810 angstroms clearly showing the frequency double prime = 0, frequency prime = 8 and frequency double prime = 0, frequency prime = 9 absorption bands. Fluorescence scans of the frequency prime = 8, frequency double prime = 1 through 24 and frequency prime = 9, frequency double prime = 1 through 25 were taken. After these broad spectra were identified, further investigations of the frequency prime = 9, frequency double prime = 6 transition included fluorescence intensity versus temperature, laser power, and pressure. Additional measurements of quenching, radiative lifetime and self absorption are made.
Download or read book U S Mining Laws and Regulations Thereunder General Land Office April 1 1879 written by and published by . This book was released on with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Progress Towards the Application of Single molecule Fluorescence Microscopy to the Study of Organometallic Reactions written by Jonathan York Bass and published by . This book was released on 2011 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chapter 1. An introduction to single molecule fluorescence microscopy is provided. Chapter 2. Initial studies demonstrated the ability to synthesize a BODIPY- tagged zirconocene complex in which the fluorescence of the tag was not quenched by the metal center and thus was suitable for single-molecule studies. Though treatment of microscope coverslips and subsequent single-molecule analysis revealed individual molecules, XPS data indicated that the complex was not binding intact to the glass surface. The binding mode of a tagged palladium complex that had been observed at the single-molecule was established by PM-FTIRRAS, suggesting the formation of a network incorporating the complex. Chapter 3. A diethylenetriamine ligand capable of being used to tether a platinum center to a glass surface for single-molecule studies of a ligand exchange reaction was synthesized. Subsequent work using amine and thiol groups tethered to a glass surface demonstrated the viability of utilizing sulfur-based ligands tethered to a glass surface for the single-molecule observation of a ligand exchange reaction at a platinum center. A thiourea ligand capable of being tethered to the surface of a glass microscope coverslip was synthesized or purified from commercial sources for use in the single-molecule ligand exchange studies. A form of BODIPY containing a triethoxysilyl group for tethering the fluorophore to the surface of a coverslip was synthesized and an optimum concentration range was found for dispersing the molecule on a coverslip surface for the determination of a delay between a molecule binding to the surface and emitting fluorescence to lead to its detection. Chapter 4. A pair of test molecules containing isolated donor and acceptor BODIPY molecules, as well as a molecule containing both donor and acceptor molecules, was synthesized for the evaluation of their feasibility as FRET partners for the single-molecule analysis of the Trost-Tsuji reaction. Single-molecule and ensemble-level studies of these molecule revealed reduced fluorescence of the FRET pair, and demonstrated the feasibility of differentiating the three types of molecules at the single-molecule level based on their fluorescence intensities by use of appropriate filter sets.
Download or read book Finding a Single Molecule in a Haystack Laser Spectroscopy of Solids from Sqrt N to N written by and published by . This book was released on 1991 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent progress in the optical detection and spectroscopy of single ions in vacuum confined in electromagnetic traps has led to novel measurements that further our understanding of quantum physics. For example, various workers have achieved direct measurement of quantum jumps, Doppler sidebands, and other fundamental phenomena such as ion crystallization of ions in vacuum trapped by electromagnetic fields. By using laser induced fluorescence and a novel hydrodynamically-focused flow to confine the molecules and reduce the scattering volume, single molecules of the protein B-phycoerythrin with the equivalent of 25 rhodamine 6G chromophores have also been detected. In contrast to the far- field optical approach, recent advances with various near-field spectroscopies such as scanning tunnelling microscopy (STM) have provided images of single molecules of benzene and CO on Rh surfaces and images of liquid crystal molecules on graphite to name a few examples.
Download or read book Laser induced Fluorescence Detection in High throughput Screening of Heterogeneous Catalysts and Single Cells Analysis written by Hui Su and published by . This book was released on 2001 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to develop laser-induced fluorescence detection in high-throughput screening of heterogeneous catalysts and single cells analysis. First, a high-throughput in situ screening approach for solid heterogeneous catalysts by laser-induced fluorescence imaging is introduced. We demonstrated LIFI has good detection performance and the spatial and temporal resolution needed for high-throughput screening of heterogeneous catalysts. We further applied the LIFI screening system for combinatorial discovery of heterogeneous catalysts and reaction condition optimization for naphthalene oxidation, as well as combinatorial study of catalytic performance of zeolites in acylation of aromatics. In the second part of this dissertation, we used laser-induced native fluorescence coupled with capillary electrophoresis (LINF-CE) and microscope imaging to study the single cell degranulation. On the basis of good temporal correlation with events observed through an optical microscope, we have identified individual peaks in the fluorescence electropherograms as serotonin released from the granular core on contact with the surrounding fluid.
