Download or read book The ELISA Guidebook written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Download or read book The Elisa Guidebook written by John R. Crowther and published by . This book was released on 2007-12-01 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA.
Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Download or read book What Are You Doing written by Elisa Amado and published by Groundwood Books Ltd. This book was released on 2017-03-01 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt: A picture book that captures a child’s discovery of the power of reading. Before he leaves for his first day of school, Chepito runs outside to play. He comes across all kinds of people in his neighborhood who are reading. “Why, why, why?” he sings, and they each have a different answer for him, whether it’s a man reading a newspaper, a young girl enjoying a comic, a couple of tourists consulting a guidebook or an archeologist studying hieroglyphics. Later that day, Chepito discovers the fun of reading for himself, and he even brings home a book to share with his little sister. Elisa Amado has written a perfect introduction to the pleasures and uses of the written word. Set in a delightfully retro world by illustrator Manuel Monroy, this book is a true celebration of reading. Correlates to the Common Core State Standards in English Language Arts: CCSS.ELA-LITERACY.RL.K.4 Ask and answer questions about unknown words in a text. CCSS.ELA-LITERACY.RL.1.2 Retell stories, including key details, and demonstrate understanding of their central message or lesson. CCSS.ELA-LITERACY.RL.2.4 Describe how words and phrases (e.g., regular beats, alliteration, rhymes, repeated lines) supply rhythm and meaning in a story, poem, or song.
Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Download or read book Fanfares and Finesse written by Elisa Koehler and published by Indiana University Press. This book was released on 2014-02-27 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: “A concise guide linking the history of trumpet to performance . . . includes information on band music, bugle calls, orchestral repertoire, and jazz.” —American Reference Books Annual Unlike the violin, which has flourished largely unchanged for close to four centuries, the trumpet has endured numerous changes in design and social status from the battlefield to the bandstand and ultimately to the concert hall. This colorful past is reflected in the arsenal of instruments a classical trumpeter employs during a performance, sometimes using no fewer than five in different keys and configurations to accurately reproduce music from the past. With the rise in historically inspired performances comes the necessity for trumpeters to know more about their instrument’s heritage, its repertoire, and different performance practices for old music on new and period-specific instruments. More than just a history of the trumpet, this essential reference book is a comprehensive guide for musicians who bring that musical history to life. “A compendium of trumpet history with short, fact-filled chapters. It will serve both amateur and professional musicians alike, and few could read this text without learning something. Fanfares and Finesse is a thorough sampling of trumpet topics, including something of interest for every trumpet player, brass enthusiast, or curious reader.” —Pan Pipes “Trumpet players in a wide variety of situations and at many levels will find a great deal of useful information, presented in a clear, engaging, reader-friendly way yet backed by solid research. While some topics are covered in more depth than others, Koehler’s breadth of vision and thoroughness are commendable . . . For all trumpeters and anyone who teaches them.” —Choice
Download or read book A Woman s Guide to Claiming Space written by Eliza VanCort and published by Berrett-Koehler Publishers. This book was released on 2023-02-21 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: For too long, women have been told to confine themselves-physically, socially, and emotionally. Eliza VanCort says now is the time for women to stand tall, raise their voices, and claim their space. Women fight the pressure to make themselves small in private, professional, and public spaces. VanCort, a teacher, consultant, and speaker, provides the necessary tools for women to rewrite the rules and create the stories of their choosing safely and without apology. VanCort identifies the five key behaviors of all Space-Claiming Queens: use your voice and posture to project confidence and power, end self-sabotage, forge connections, neutralize unsafe spaces, and unite across differences. Through personal narrative, research, and actionable strategies, VanCort provides how-tos on combating challenges, such as antimentors and microaggressions, and gives advice for building up your old girls club, asking for what you're worth, and owning your space without apology. Bold, fun, and enlightening, this book is birthed from VanCort's incredible story. Having a mother with schizophrenia forced VanCort to learn to be small and invisible at an early age, and suffering a traumatic brain injury as an adult required her to rethink communication from the ground up. Drawing on these experiences, and those of real women everywhere, VanCort empowers women to claim space for themselves and for their sisters with courage, empathy, and conviction because when we rise together, we rise so much higher.
Download or read book Elisa s Diary written by Elisa Velasquez and published by Createspace Independent Publishing Platform. This book was released on 2017-02-11 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Excerpts from Elisa's personal diary of her conversations with God. I'm always writing, God is constantly talking and I'm always writing. This book is uniquely wonderful and penned with my heart. I love sharing what I write and the words that God whispers in my ear. It's important to build people up and inspire and even correct. This diary does just that. Elisa spraks to your spirit heart and delivers God's Messages purely and honest. Listen to God's word. Elisa's Diary are excerpts from her daily personal God diary. Keeping a diary is most rewarding and memorable. Your thoughts are probably the best legacies to leave behind on earth.
Download or read book Microbiology Laboratory Guidebook written by United States. Food Safety and Inspection Service. Microbiology Division and published by . This book was released on 1998 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme linked Immunosorbent Assay ELISA written by Samira Hosseini and published by Springer. This book was released on 2017-12-30 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
Download or read book The Enzyme Linked Immunosorbent Assay Elisa written by A. Voller and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Download or read book The Time Travel Handbook written by James Wyllie and published by Profile Books. This book was released on 2015-10-29 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Not many of us can claim to have dipped our handkerchiefs in Charles I's blood after his execution, or to have watched Vesuvius erupt, but that's about to change... Wyllie, Acton & Goldblatt's Time Travel Handbook offers eighteen exceptional trips to the past, transporting you back to the greatest spectacles in history. We offer the chance to join Henry VIII at the Field of the Cloth of Gold, and to march on Versailles with the revolutionary women of Paris. You can sail with Captain Cook to Tahiti and Australia, and spend time at Xanadu with Marco Polo and Kubla Khan. Or, closer to the present, you might accompany Charlie Parker at the birth of bebop or The Beatles in Hamburg, and take part in the VE Day celebrations in London or the Fall of the Berlin Wall. The notable authors and time travel agents, Wyllie, Acton & Goldblatt are your guide to these and other unmissable events, charting the action as it will unfold, and advising on local customs, and what to wear, eat and drink, for the most authentic of experiences. Forget museums, forget history books - the only way to do history is to live it.
Download or read book New Mexico written by Elisa Parhad and published by Eye Muse. This book was released on 2012-02-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Visually focused, packed with cultural insight, and sized for portability, Guides for the Eyes celebrate the local traditions and visual vernacular that surrounds us. Operating at the intersection of art and anthropology, each edition in the series explores place through 100 of the most notable features of a region. Vivid photography and informative text provide insight into the significance of each topic, covering regional architecture, design, flora, fauna, food, crafts, folklore, landscape, and other facets of local identity and style. Ranging from the obvious to the obscure, these distinguishing elements define a locale as somewhere as opposed to anywhere. As one of the most unique and colorful regions of North America, New Mexico is the first subject of the series. Throughout thousands of years, the state s striking landscape has inspired the art, design and traditions of its three dominant cultures: Native American, Hispanic and Anglo/Western. These distinct groups continue to blend and evolve, infusing the rustic land with a compelling vibrancy, unrivaled in its beauty.
Download or read book Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis written by Jussi Meriluoto and published by John Wiley & Sons. This book was released on 2017-01-30 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: A valuable handbook containing reviews, practical methods and standard operating procedures. A valuable and practical working handbook containing introductory and specialist content that tackles a major and growing field of environmental, microbiological and ecotoxicological monitoring and analysis Includes introductory reviews, practical analytical chapters and a comprehensive listing of almost thirty Standard Operating Procedures (SOPs) For use in the laboratory, in academic and government institutions and industrial settings Those readers will appreciate the research that validates and updates cyanotoxin monitoring and analysis plus adding to approaches for setting standard methods that can be applied worldwide. Wayne Carmichael, Analytical and Bioanalytical Chemistry (2018)
