Download or read book The Effects of Macrocyclic Constraints on Electron Transfer in Peptides written by John R. Horsley and published by . This book was released on 2015 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research undertaken in this thesis focuses on electron transfer in peptides constrained into either a 310-helical or a [beta]-strand conformation in order to progress the field of molecular electronics. Chapter One: Natural proteins have evolved to promote electron transfer in many biological processes. However, their complex conformational nature inhibits a thorough investigation, so in order to study electron transfer in proteins, simple peptide models containing redox active moieties present as ideal candidates. Chapter One introduces the importance of secondary structure characteristic to proteins/peptides, and its relevance to electron transfer. The proposed mechanisms responsible for such electron transfer are discussed, along with the various approaches used to further constrain the peptides into their geometric conformations. The methods used to characterize the conformation of all peptides synthesized throughout this thesis are outlined, as are details of the electrochemical techniques used to investigate their electronic properties. A literature review describing several factors that have been shown to influence electron transfer in peptides, and a brief summary of molecular electronics follows. Chapter Two: Two 310-helical peptides were synthesized, one constrained via a covalent side-chain staple using Huisgencycloaddition, and the other a linear analogue. Both peptides contain a redox active terminal ferrocene moiety, and were separately attached to a single walled carbon nanotube (SWCNT)/gold electrode array for electrochemical analysis. The effect of backbone rigidity imparted by the side-bridge constraint was revealed, which was shown to restrict the necessary torsional motions that lead to facile intramolecular electron transfer along the peptide backbone. High level calculations were used to support the electrochemical observations. Chapter Three: A series of peptides constrained into either a 310-helix or [beta]-strand conformation were synthesized, each containing a varied number of electron rich alkene side chains. The ability of the alkene(s) to facilitate electron transfer through the peptides by exploiting a hopping mechanism, and thus act as a "stepping stone" was investigated. Ring closing metathesis was used to further rigidify the backbones of a helical and a [beta]-strand peptide via side chain tethers. The ensuing saturated and unsaturated compounds were electrochemically interrogated in order to explore any possible interplay between the effects of the alkene side-chains and backbone rigidity. High level calculations were conducted to verify the observed electrochemical data. Chapter Four: Two [beta]-strand peptides were synthesized, one constrained via a covalent side-chain staple using Huisgen cycloaddition, and the other a linear analogue. Both peptides contain a redox active terminal ferrocene moiety, and were separately attached to a SWCNT/gold electrode array for electrochemical analysis. The charge transfer pathway was determined to be intramolecular by measuring the electron transfer rate at various concentrations of the constrained peptide bound to the electrode. This pathway is analogous to charge transfer through a molecular junction involving a single peptide. Theoretical conductance simulations were then undertaken using two peptide analogues in order to establish a link between the electrochemical observations and conductance measurements through a molecular junction. Chapter Five: Two macrocyclic peptides were synthesized, one constrained into a 310-helical conformation by linking its i to i+3 residues to form a lactam bridge, and the other constrained into a [beta]- strand geometry via a lactam-bridge tether, linking its i to i+2 residues. These peptides were chosen in order to define the role of the amide bond in a lactam bridge constraint. Direct linear analogues of each were used to establish the effect on electron transfer from a terminal amide bond located in an untethered side-chain. High level calculations were also conducted in order to elucidate the mechanism(s) responsible for electron transfer in each of the linear and macrocyclic helical peptides.
Download or read book Peptides and Peptide based Biomaterials and their Biomedical Applications written by Anwar Sunna and published by Springer. This book was released on 2017-10-26 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid-binding peptides have been used increasingly as molecular building blocks in nanobiotechnology as they can direct the assembly and functionalisation of a diverse range of materials and have the ability to regulate the synthesis of nanoparticles and complex nanostructures. Nanostructured materials such as β-sheet fibril-forming peptides and α-helical coiled coil systems have displayed many useful properties including stimulus-responsiveness, modularity and multi-functionality, providing potential technological applications in tissue engineering, antimicrobials, drug delivery and nanoscale electronics. The current situation with respect to self-assembling peptides and bioactive matrices for regenerative medicine are reviewed, as well as peptide-target modeling and an examination of future prospects for peptides in these areas.
Download or read book Stability of and Electron Transfer Through Self assembled Monolayers of Conformationally Constrained Peptides written by and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Transfer and Folding Studies in Designed Three helix Bundle Peptides written by Yongjian Zheng and published by . This book was released on 2003 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2005 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Peptide Macrocycles written by Matthew B. Coppock and published by Humana. This book was released on 2021-11-02 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques and strategies used to study the field of peptide macrocycles. The chapters in this book ae organized into four parts: macrocycles synthesis, combinational library synthesis and screening, macrocycle characterization, and unique applications. Part One looks at a variety of peptide cyclization methodologies, and Part Two describes methods for the creation of peptide macrocycles libraries and their subsequent screening against biological targets of interest. Part Three discusses the study and characterization of peptide macrocycle-target interactions, and Part Four introduces unique applications for peptide macrocycles, from higher-order structure formation to post-synthetic functional modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Peptide Macrocycles: Methods and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this developing field.
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2734 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2036 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Successful Strategies for the Discovery of Antiviral Drugs written by Manoj C. Desai and published by Royal Society of Chemistry. This book was released on 2013 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume will be aimed at medicinal chemistry and emerging drug discovery scientists.
Download or read book Directory of Graduate Research written by American Chemical Society. Committee on Professional Training and published by . This book was released on 2005 with total page 1932 pages. Available in PDF, EPUB and Kindle. Book excerpt: Faculties, publications and doctoral theses in departments or divisions of chemistry, chemical engineering, biochemistry and pharmaceutical and/or medicinal chemistry at universities in the United States and Canada.
Download or read book Molecular Modeling of Inorganic Compounds written by Peter Comba and published by John Wiley & Sons. This book was released on 2009-07-10 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: After the second edition introduced first density functional theory aspects, this third edition expands on this topic and offers unique practice in molecular mechanics calculations and DFT. In addition, the tutorial with its interactive exercises has been completely revised and uses the very latest software, a full version of which is enclosed on CD, allowing readers to carry out their own initial experiments with forcefield calculations in organometal and complex chemistry.
Download or read book Research Awards Index written by and published by . This book was released on 1987 with total page 852 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Peptide Therapeutics written by Ved Srivastava and published by Royal Society of Chemistry. This book was released on 2019-08-28 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide therapy has become a key strategy in innovative drug development, however, one of the potential barriers for the development of novel peptide drugs in the clinic is their deficiencies in clearly defined chemistry, manufacturing and controls (CMC) strategy from clinical development to commercialization. CMC can often become a rate-limiting step due to lack of knowledge and lack of a formal policy or guidelines on CMC for peptide-based drugs. Regulators use a risk-based approach, reviewing applications on a case-by-case basis. Peptide Therapeutics: Strategy and Tactics for Chemistry, Manufacturing, and Controls covers efficient manufacturing of peptide drug substances, a review of the process for submitting applications to the regulatory authority for drug approval, a holistic approach for quality attributes and quality control from a regulatory perspective, emerging analytical tools for the characterisation of impurities, and the assessment of stability. This book is an essential reference work for students and researchers, in both academia and industry, with an interest in learning about CMC, and facilitating development and manufacture of peptide-based drugs.
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1991 with total page 1104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Summaries of Projects Completed in Fiscal Year written by National Science Foundation (U.S.) and published by . This book was released on 1979 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1840 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Book of Abstracts written by and published by . This book was released on 2000 with total page 778 pages. Available in PDF, EPUB and Kindle. Book excerpt:
