Download or read book The Effect of Pure Titanium and Titanium Alloy on Osteoblast and Chondrocyte Maturation in Vitro written by Jack Herbert Lincks and published by . This book was released on 1999 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Effect of Pure Titanium and Titanium Alloy on Osteoblast and Chondrocyte Maturatin In Vitro written by Jack Lincks and published by . This book was released on 1999 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: Implant success is dependent on the interaction of the implant with surrounding tissues. By understanding this, better devices can be developed. Substrate specific information regarding implant surface characteristics and its influence on mesenchymal cell proliferation and differentiation may be helpful in the design of implants to optimize bone growth at the interface. Based on in vivo and in vitro studies, biomaterial composition, surface energy, surface roughness, and surface topography appear to play a major role in implant success. However, in-depth analysis of cellular response to various implant surface characteristics and the ensuing local and systemic factor production remain to be performed. The first aim of this study was to evaluate the ability of two implant characteristics, chemical composition and surface roughness, to modulate various parameters of osteoblast metabolism in vitro. Commercially pure titanium (Ti) and titanium-6 wt.% aluminum-4 wt.% vanadium alloy (Ti-A) disks were machined to achieve a specific roughness through either fine-polishing (S, smooth, Ra 0.2 micronmeter) or wet sand-grinding (R, rough, Ra 3.0 micronmeter). Profilometry and scanning electron microscopy (SEM) were used to determine surface roughness, Auger spectroscopy and FT-IR were also used to characterize the disks. MG63 osteoblast-like human osteosarcoma cells were seeded at 9300 cells/cm2 and cultured to confluence on standard tissue culture polystyrene or the Ti surfaces. Cell morphology was assessed by SEM. Osteoblast cultures were examined for differences in proliferation as measured by cell number, and 3H-thymidine incorporation; differentiation by cell and cell layer alkaline phosphatase specific activity (ALpase) and osteocalcin production; matrix synthesis by proteoglycan sulfation and % collagen production; and cytokine production by transforming growth factor-Beta and prostaglandin E2 production.
Download or read book The Effect of Plasma Treatment of the Surface of Commercially Pure Titanium on the Degree of Cellular Spreading of Osteoblasts written by John Peter Joseph Fearon and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biological Activity of Osteoblasts on Nickel titanium and Titanium Alloy Surfaces in Vitro written by Douglas J. Wood and published by . This book was released on 1998 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Induction of Bone Resorbing Agents by Titanium Particulates written by TT. Glant and published by . This book was released on 1996 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although titanium and titanium alloys demonstrate excellent biocompatability in bulk form, titanium in particulate form can provoke a variety of cellular responses. In this series of experiments, we have shown that commercially pure titanium particulates of phagocytosable size stimulate the secretion of mediators of bone resorption (prostaglandin E2 and interleukin-1) from macrophages and cause bone resorption in organ culture. In addition, we have shown that phagocytosable titanium particles stimulate fibroblasts to up regulate the expression of matrix metalloproteinases (stromelysin and collagenase) without a substantial effect on the tissue inhibitor of these enzymes (TIMP). Titanium particulates also have a suppressive effect on procollagen synthesis by an osteoblast-like cell line. Thus, titanium particulates have the capacity to stimulate bone resorption and inhibit bone matrix formation.
Download or read book The Role of Nanostructural and Electrical Surface Properties on the Osteogenic Potential of Titanium Implants written by Rolando Arturo Gittens Ibacache and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Dental and orthopaedic implants are currently the solutions of choice for teeth and joint replacements with success rates continually improving, but they still have undesirable failure rates in patients who are compromised by disease or age, and who in many cases are the ones most in need. The success of titanium (Ti) implants depends on their ability to osseointegrate with the surrounding bone and this, in turn, is greatly dependent on the surface characteristics of the device. Advancements in surface analysis and surface modification techniques have improved the biological performance of metallic implants by mimicking the hierarchical structure of bone associated with regular bone remodeling. In this process, damaged bone is resorbed by osteoclasts, which produce resorption lacunae containing high microroughness generated after mineral dissolution under the ruffled border, as well as superimposed nanoscale features created by the collagen fibers left at the surface. Indeed, increasing Ti surface roughness at the micro and sub-microscale level has been shown to increase osteoblast differentiation in vitro, increase bone-to-implant contact in vivo, and accelerate healing times clinically. Recently, the clinical application of surface nanomodification of implants has been evaluated. Still, most clinically-available devices remain smooth at the nanoscale and fundamental questions remain to be elucidated about the effect of nanoroughness on the initial response of osteoblast lineage cells. \r : Another property that could be used to control osteoblast development and the process of osseointegration is the electrical surface charge of implants. The presence of endogenous electrical signals in bone has been implicated in the processes of bone remodeling and repair. The existence of these native signals has prompted the use of external electrical stimulation to enhance bone growth in cases of fractures with delayed union or nonunion, with several in vitro and in vivo reports confirming its beneficial effects on bone formation. However, the use of electrical stimulation on Ti implants to enhance osseointegration is less understood, in part because of the lack of in vitro models that truly represent the in vivo environment. In addition, an aspect that has not been thoroughly examined is the electrical implication of implant corrosion and its effect on the surrounding tissue. Implants are exposed to extreme conditions in the body such as high pH during inflammation, and cyclic loads. These circumstances may lead to corrosion events that generate large electrochemical currents and potentials, and may cause abnormal cell and tissue responses that could be partly responsible for complications such as aseptic loosening of implants. \r : Consequently, Ti implants with tailored surface characteristics such as nanotopography and electrical polarization, could promote bone healing and osseointegration to ensure successful outcomes for patients by mimicking the biological environment of bone without the use of systemic drugs. The objective of this thesis is to understand how surface nanostructural and electrical characteristics of Ti and Ti alloy surfaces may affect osteoblast lineage cell response in vitro for normal tissue regeneration and repair. Our central hypothesis is that combined micro/nanostructured surfaces, as well as direct stimulation of Ti surfaces with fixed direct current (DC) potentials, can enhance osteoblast differentiation.
Download or read book In Vitro Osteoblast Responses to CpTi and Ti 6Al 4V Alloy written by RA. Draughn and published by . This book was released on 1996 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt: Commercially pure Ti and Ti-6Al-4V have been widely used as dental implant materials. Several reports have demonstrated that there are subtle differences in the nature of the oxide between these two materials which may have important biological consequences. An earlier report by our group demonstrated differences in oxide chemistry between cpTi and Ti alloy does not affect short term in vitro responses of osteoblast cultures. In this work, long term phenotypic expression of typical bone cell markers such as osteocalcin and alkaline phosphatase were not different between metal or control surfaces. There was a slight difference in total Ca+2 per culture for alloy compared to cpTi, however, these differences were not statistically different. Based on this work, both cpTi and Ti alloy surfaces support in vitro biological mineralization and are in general agreement with in vivo findings.
Download or read book Osteoblasts Response to Anodized Commercially Pure Titanium in Vitro written by Jun Chen and published by . This book was released on 2005 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Role of Surface Chemistry and Wettability of Microtextured Titanium Surfaces in Osteoblast Differentiation written by Jung Hwa Park and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomaterial surface energy, chemical composition, charge, wettability and roughness all play an important role in determining the degree of the direct bone-to-implant interface, termed osseointegration. Surface chemistry, which is influenced by surface energy, wettability, and composition, is another factor that determines osteoblast phenotype and regulates osteoblast maturation. Increased surface energy is desirable for bone implants due to enhanced interaction between the implant surface and the biological environment. The extent of bone formation in vivo is also increased with increasing water wettability of implants. The physiological role of implant surface chemistry is important in determining the success of implant osseointegration because of molecular rearrangements, surface reactions, contamination, and release of toxic or biologically active ions that are determined by the starting chemistry. However, the role of surface chemistry on osteoblast response is not fully studied. Therefore, the overall goal of this dissertation is to understand how the surface chemistry, including wettability, chemical composition, and charge density, of titanium biomaterials impacts osteoblast maturation (in vitro). This study focuses on the general hypothesis that modifications of surface chemistry of titanium surfaces with sterilization or polyelectrolyte coating on titanium surfaces regulate osteoblast response.
Download or read book Phytic Acid Calcium Hydroxide Composite Coating on Titanium Implants Promote Early Osseointegration written by Hao Zhang and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract:Titanium surfaces have been constantly modified to improve bioactivity and osseointegration. In this study, we modified titanium surfaces through hydrothermal treatment with a mixed solution of phytic acid and calcium hydroxide, then evaluated their bioactive and osteogenic activities. In vitro tests, calcium-decorated titanium surface enhanced the cell adhesion, proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs). In vivo tests, Micro- CT and histological evaluations indicated that the modified implants promoted new bone formation and osseointegration between the implants and the host bone. These findings provided evidence that this simple modification would be promising solution to improve bioactivity and osseointegration, which would be beneficial not only for titanium implants but also other implanted biomaterials.Background: Titanium (Ti) or titanium alloys coated with various bioactive calcium phosphates (CaPs), including hydroxyapatite (HA) and u03b2-tricalcium phosphate (u03b2-TCP), are considered as satisfactory implant materials. CaP coatings provide nanostructural or microstructuctural characteristics on titanium or titanium alloys, thereby increasing the adhesion of osteoblasts, which enhance the osseointegration and shorten the healing time.Aim: To prepare and evaluate the effect of calcium-containing chemical conversion coating on promoting new bone formation and early osseointegration in vivo.Material and methods: We designed and prepared a phytic acid/calcium hydroxide composite coating on the pure titanium implant (Ti-SLA-2/10) through hydrothermal treatment with a mixed solution of phytic acid (PA) and saturated calcium hydroxide solution [Ca (OH)2]. The pure titanium implant with a sandblasted/acid-etched (SLA) surface was used as the control group (Ti-SLA). The surface morphology, elemental composition, surface roughness, wettability, and calcium ion release were investigated separately. Twenty-four SLA and calcium-containing SLA implants were immediately inserted into the mandibles of twelve New Zealand white rabbits. The biological effects were evaluated by Micro-computed tomography (Micro-CT) analysis and histological evaluation after 1, 2, and 4 weeks of healing. Results: Phytic acid/calcium hydroxide composite coating with superior hydrophilicity were successfully prepared on titanium implants. The surface characteristics showed Ti-SLA-2/10 implants with multiple pits and craters can release calcium ions continuously. In vivo, the volume of new bone around the Ti-SLA-2/10 implants was significantly higher than that of the Ti-SLA implants, but more fibrous tissue was observed surround the Ti-SLA implants after 1 week of healing. The Ti-SLA-2/10 implants showed higher bone volume fraction (bone volume/total volume, BV/TV, %) than that of the Ti-SLA implants at each time points (p
Download or read book Synergistic Effect of Titanium Alloy and Collagen Type I on Cell Adhesion Proliferation and Differentiation of Osteoblast Like Cells written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioscience and Bioengineering of Titanium Materials written by Yoshiki Oshida and published by Elsevier. This book was released on 2010-07-07 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book about bioscience and the bioengineering of titanium materials is based on more than 1,000 published articles. It bridges the gap between the medical/dental fields and the engineering/technology areas, due to the author’s unique experience in both during the last 30 years. The book covers Materials Classifications, Chemical and Electrochemical Reactions, Oxidation, Biological Reactions, Implant-related Biological Reactions, Applications, Fabri-cation Technologies, Surface Modifications, and Future Perspectives. * Provides quick access to the primary literature in this field* Reviews studies of titanium materials in medical and dental applications, as reported in nearly 1,500 articles published over last several years* Draws information from several types of studies and reports* Helps readers answer questions about the most appropriate materials and when to use them
Download or read book The Effect of Pure Titanium on the Growth of Plaque Organisms in Vitro written by Shackleton Joy Linda and published by . This book was released on 1988 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1856 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface Coating and Modification of Metallic Biomaterials written by Cuie Wen and published by Woodhead Publishing. This book was released on 2015-03-31 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite advances in alternative materials, metals are still the biomaterial of choice for a number of clinical applications such as dental, orthopedic and cardiac implants. However, there are a number of intrinsic problems associated with implanting metal in the biological environment, such as wear, corrosion, biocompatibility and toxicity, which must be addressed. Modern technology has enabled scientists to modify metal surfaces or apply special coatings to metals to improve their performance safety. Surface Coating and Modification of Metallic Biomaterials will discuss the most important modification techniques and coatings for metals, first covering the fundamentals of metals as a biomaterial and then exploring surface modification techniques and coatings. An expansive overview of surface modification techniques for biomedical use In-depth exploration of issues arising from metal biomaterial use Includes examples of applications in a clinical setting
Download or read book Bone Tissue Engineering written by Jeffrey O. Hollinger and published by CRC Press. This book was released on 2004-10-14 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t
Download or read book Smart Biomaterials written by Mitsuhiro Ebara and published by Springer. This book was released on 2014-05-28 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides comprehensive coverage of smart biomaterials and their potential applications, a field that is developing at a very rapid pace. Because smart biomaterials are an emerging class of biomaterials that respond to small changes in external stimuli with large discontinuous changes in their physical properties, they have been designed to act as an “on–off” switch for, among others, bio separation, immunoanalysis, drug delivery technologies, gene therapy, diagnostics, bio sensors and artificial muscles. After an introduction to the topic and the history of smart biomaterials, the author gives the reader an in-depth look at the properties, mechanics, and characterization of smart biomaterials including hydrogels, particles, assemblies, surfaces, fibers and conjugates. Information on the wide range of applications for these materials follows, including drug delivery, tissue engineering, diagnostics, biosensors, bio separation and actuators. In addition, recent advances in shape memory biomaterials as active components of medical devices are also presented.
