Download or read book The Effect of Protein Concentration and Ionic Strength on the Diffusion Coefficient of Bovine Serum Albumin written by Francis Rauh and published by . This book was released on 1976 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization by Optical Methods of the Heat Denaturation of Bovine Serum Albumin BSA as Affected by Protein Concentration PH Ionic Strength and Sugar Concentration written by Teepakorn Kongraksawech and published by . This book was released on 2007 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: The thermal denaturation of proteins has been extensively studied using several methods including differential scanning calorimetry (DSC). A custom-built optical system was used to study thermal effects on protein as an alternative method to DSC measurements. It was used to investigate the thermal stability of bovine serum albumin (BSA) with a focus on comparisons with published DSC data. In the first study, the effect of protein concentration on the thermal denaturation (Td) of BSA was determined and validated using published DSC data for bovine serum albumin (BSA). The optical rotation (OR) and transmitted light (TL) signals indicating protein conformational changes and gel formation, respectively, were collected during the heating of BSA solutions at [approximately] 6°C/min from room temperature to [approximately] 85°C. The experiments were performed on 1, 2.5 and 5% (w/v) BSA in 0.01 M phosphate buffer at pH 7 and ionic strength (IS) 0.08. BSA's Td values obtained from this investigation were consistent with published values and had low experimental variability (CV
Download or read book Masters Theses in the Pure and Applied Sciences written by Wade H. Shafer and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Masters Theses in the Pure and Applied Sciences was first conceived, published, and dis seminated by the Center for lnformation and Numerica/ Data Analysis and Synthesis (C/NDAS) * at Purdue University in 1957, starting its coverage of theses with the academic year 1955. Beginning with Volume 13, the printing and dissemination phases of the ac tivity were transferred to University Microfilms/Xerox of Ann Arbor, Michigan, with the thought that such an arrangement would be more beneficia! to the academic and general scientific and technical community. After fi ve years of this joint undertaking we had concluded that it was in the interest of ali concerned if the printing and distribution of the volume were handled by an international publishing house to assure improved service and broader dissemination. Hence, starting with Volume 18, Masters Theses in the Pure and App/ied Sciences has been disseminated on a worldwide basis by Plenum Publishing Corporation of New York, and in the same year the coverage was broadened to include Canadian universities. Ali back issues can also be ordered from Plenum. We have reported in Volume 21 (thesis year 1976) a total of 10,586 theses titles from 25 Canadian and 219 United States universities. We are sure that this broader base for theses titles reported will greatly enhance the value of this important annual reference work.
Download or read book The Ultrafiltration of Bovine Serum Albumin written by William Dexter Munch and published by . This book was released on 1977 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1977 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Colloid and Interface Science V5 written by Milton Kerker and published by Elsevier. This book was released on 2012-12-02 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: Colloids and Interface Science, Volume V: Biocolloids, Polymers, Monolayers, Membranes, and General Papers is a collection of manuscripts presented at the International Conference on Colloids and Surfaces, held in San Juan, Puerto Rico on June 21-25, 1976. The conference is sponsored jointly by the Division of Colloid and Surface Chemistry of the American Chemical Society and the International Union of Pure and Applied Chemistry in celebration of the 50th Anniversary of the Division and the 50th Colloid and Surface Science Symposium. This volume is organized into 51 chapters that cover the subjects of biocolloids, polymers, monolayers, membranes, and general papers. Other topics discussed include the thermodynamic aspects of biocolloids; adsorption of polymers; ion-exchange behavior; optical properties of polymers; microvoid films; micellar systems; and liquid crystals. The remaining chapters explore the diffusion and sorption of simple ions, surface properties of copolymers, and adhesion of thermoplastic elastomers.
Download or read book The Biochemical Journal written by and published by . This book was released on 1956 with total page 854 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Masters Theses in the Pure and Applied Sciences written by W. H. Shafer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: Masters Theses in the Pure and Applied Sciences was first conceived, published, and dis seminated by the Center for Information and Numerical Data Analysis and Synthesis (CINDAS) * at Purdue University in 1957, starting its coverage of theses with the academic year 1955. Beginning with Volume 13, the printing and dissemination phases of the ac tivity were transferred to University Microfilms/Xerox of Ann Arbor, Michigan, with the thought that such an arrangement would be more beneficial to the academic and general scientific and technical community. After five years of this joint undertaking we had concluded that it was in the interest of all concerned if the printing and distribution of the volume were handled by an international publishing house to assure improved service and broader dissemination. Hence, starting with Volume 18, Masters Theses in the Pure and Applied Sciences has been disseminated on a worldwide basis by Plenum Publishing Corporation of New York, and in the same year the coverage was broadened to include Canadian universities. All back issues can also be ordered from Plenum. We have reported in Volume 22 (thesis year 1977) a total of 10,658 theses titles from 28 Canadian and 227 United States universities. We are sure that this broader base for theses titles reported will greatly enhance the value of this important annual reference work. While Volume 22 reports theses submitted in 1977, on occasion, certain universities do report theses submitted in previous years but not reported at the time.
Download or read book Dynamic Light Scattering written by R. Pecora and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the twenty years since their inception, modern dynamic light-scattering techniques have become increasingly sophisticated, and their applications have grown exceedingly diverse. Applications of the techniques to problems in physics, chemistry, biology, medicine, and fluid mechanics have prolifer ated. It is probably no longer possible for one or two authors to write a monograph to cover in depth the advances in scattering techniques and the main areas in which they have made a major impact. This volume, which we expect to be the first of aseries, presents reviews of selected specialized areas by renowned experts. It makes no attempt to be comprehensive; it emphasizes a body of related applications to polymeric, biological, and colloidal systems, and to critical phenomena. The well-known monographs on dynamic light scattering by Berne and Pecora and by Chu were published almost ten years ago. They provided comprehensive treatments of the general principles of dynamic light scat tering and gave introductions to a wide variety of applications, but natu rally they could not treat the new applications and advances in older ones that have arisen in the last decade. The new applications include studies of interacting particles in solution (Chapter 4); scaling approaches to the dynamics of polymers, including polymers in semidilute solution (Chapter 5); the use of both Fabry-Perot interferometry and photon correlation spectroscopy to study bulk polymers (Chapter 6); studies of micelIes and microemulsions (Chapter 8); studies of polymer gels (Chapter 9).
Download or read book A Study of the Diffusion of Bovine Serum Albumin at the Oil water Interface Using Total Internal Reflection Fluorescence and Fluorescence Photobleaching Recovery written by Parijat Jauhari and published by . This book was released on 1998 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Membrane Fouling During Hollow Fiber Ultrafiltration of Protein Solutions written by Amin Reza Rajabzadeh and published by . This book was released on 2011 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hollow fiber ultrafiltration is a viable low cost alternative technology for the concentration or separation of protein solutions. However, membrane fouling and solute build up in the vicinity of the membrane surface decrease the performance of the process by lowering the permeate flux. Major efforts have been devoted to study membrane fouling and design more efficient ultrafiltration membrane systems. The complexity of membrane fouling, however, has limited the progress to better understand and predict the occurrence of fouling. This work was motivated by the desire to develop a microscopic Computational Fluid Dynamics (CFD) model to capture the complexity of the membrane fouling during hollow fiber ultrafiltration of protein solutions. A CFD model was developed to investigate the transient permeate flux and protein concentration and the spatial fouling behavior during the concentration of electroacidified (pH 6) and non- electroacidified (pH 9) soy protein extracts by membrane ultrafiltration. Electroacidification of the soy protein to pH 6 was found to decrease the permeate flux during UF which resulted in longer filtration time. Lower electrostatic repulsion forces between the proteins at pH 6 (near the protein isoelectric point) resulted in a tighter protein accumulation on the membrane surface suggested to be responsible for the lower permeate flux observed in the UF of the electroacidified soy protein extract. A new transient two-component fouling resistance model based on the local pressure difference, permeate velocity and protein concentration was implemented in the resistance-in-series flux model to describe the dynamics of the reversible and irreversible fouling during the filtration and the effect of pH on the membrane fouling. Good agreement between the experimental data and the model predictions was observed. Mathematical modeling was performed to estimate the osmotic pressure and diffusion coefficient of the proteins bovine serum albumin (BSA) and soy glycinin, one of the major storage proteins in soy, as a function of protein concentration, pH, and ionic strength. Osmotic pressure and diffusion coefficient of proteins play vital roles in membrane filtration processes because they control the distribution of particles in the vicinity of the membrane surface, often influencing the permeation rate. Therefore, understanding the behavior of these properties is of great importance in addressing questions about membrane fouling. An artificial neural network was developed to analyze the estimated data in order to find a simple relation for osmotic pressure as a function of protein concentration, pH, and ionic strength. For both proteins, the osmotic pressure increased as pH diverged from the protein isoelectric point. Increasing the ionic strength, however, reversed the effect by shielding charges and thereby decreasing the osmotic pressure. Osmotic pressure of glycinin was found lower than that of BSA. Depending on how much pH was far from the isoelectric point of the protein, osmotic pressure of BSA could be up to three times more than the glycinin's. Two different trends for diffusion coefficient at specified pH and ionic strength were observed for both proteins; diffusion coefficient values that decreased with protein concentration and diffusion coefficient values that passed through a maximum. A rigorous CFD model based on a description of protein interactions was developed to predict membrane fouling during ultrafiltration of BSA. BSA UF was performed in a total recycle operation mode in order to maintain a constant feed concentration. To establish a more comprehensive model and thereby alleviate the shortcomings of previous filtration models in literature, this model considered three major phenomena causing the permeate flux decline during BSA ultrafiltration: osmotic pressure, concentration polarization, and protein adsorption on the membrane surface. A novel mathematical approach was introduced to predict the concentration polarization resistance on the membrane. The resistance was estimated based on the concentration and thickness profile of the polarization layer on the membrane obtained from the solution of the equation of motion and continuity equation at a previous time step. Permeate flux was updated at each time step according to the osmotic pressure, concentration polarization resistance, and protein adsorption resistance. This model had the ability to show how microscopic phenomena such as protein interactions can affect the macroscopic behaviors such as permeate flux and provided detailed information about the local characteristics on the membrane. The model estimation was finally validated against experimental permeate flux data and good agreement was observed.
Download or read book Cambridge Scientific Biochemistry Abstracts written by and published by . This book was released on 1985-07 with total page 862 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advances in Colloid Science written by Mohammed Rahman and published by BoD – Books on Demand. This book was released on 2016-11-23 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book Advances in Colloid Science covers a number of up-to-date research advancement and progresses on colloids. It is a promising novel research field that has acknowledged a lot of interest recently. Here, the exciting scientific reports on cutting edge of science and technology associated to facile and economical synthesis, self-assembly, wettability, liquid crystallinity, physical properties, adoptions, morphology, control, drug design, structural properties, and prospective biological and optical implementation of newly designed colloids are concluded. This book presents an overview of recent and current colloidal study of fundamental and significant applications and implementation research worldwide. The colloidal science offers significant new and exciting challenges in biomedical, chemical, physical, and technological field. It is an important booklet for research organizations, governmental research centers, academic libraries, and R
Download or read book A Hydrodynamic Study of the Configuration of Bovine Serum Albumin written by George Irwin Loeb and published by . This book was released on 1956 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effects of PH Ionic Strength Protein Concentration and Disulfide Bond Cleavage on the Formation and Coalesence Stability of Emulsions Stabilized by Different Milk Proteins written by Joseph Leonard Klemaszewski and published by . This book was released on 1990 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Journal of Chemical Physics written by and published by . This book was released on 1982-04 with total page 992 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrophoretic Fractionation of Proteins written by Alice Sheng Chiang Chu and published by . This book was released on 1985 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt:
